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| Registros recuperados : 106 | |
| 101. |  | BRITO, L. G.; VIEIRA JUNIOR, J. R.; BARBIERI, F. da S.; ROCHA, R. B.; FIGUEIRO, M. R.; SILVA, W. C. da; CARVALHO, G. L. O.; SILVA, J. de A.; SOUZA, G. N. de. Evaluation of Milk Compositional Quality and Mammary Gland Health of Dairy Herds in the Southwestern Brazilian Amazon. Open Journal of Veterinary Medicine, n. 6, p. 139-148, 2016.| Biblioteca(s): Embrapa Rondônia. |
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| 102. | | TANAKA, E. V.; MORENO, R. V.; GONÇALVES, T. C.; FANATTO, R. R.; NÉO, R. A.; BILHASSI, T. B.; GIGLIOTI, R; RABELO, M. D.; BRITO, L. G.; BARBIERI, F. da S.; OLIVEIRA, M. C. de S. Avaliação da resistência a pesticidas piretróides em populações de Rhipicephalus microplus. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 6., 2014, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação: Embrapa Pecuária Sudeste, 2014. p. 109. (Embrapa Instrumentação. Documentos, 57) Editores técnicos: João de Mendonça Naime, Caue Ribeiro, Maria Alice Martins, Elaine Cristina Paris, Paulino Ribeiro Villas Boas, Ladislau Marcelino Rabello.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 103. | | BRITO, L. G.; ROCHA, R. B.; BARBIERI, F. da S.; RIBEIRO, E. S.; VENDRAMI, F. B.; SOUZA, G. C. R.; GIGLIOTI, R.; REGITANO, L. C. de A.; FALCOSKI, T. O. R. S.; TIZIOTO, P. C.; OLIVEIRA, M. C. de S. Babesia bovis infection in cattle in the southwestern Brazilian Amazon. Ticks and Tick-borne Diseases, v. 4, n.1/2, p. 78-82, 2013.| Biblioteca(s): Embrapa Pecuária Sudeste; Embrapa Rondônia. |
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| 104. | | ALVES, R. de C.; VIEIRA JUNIOR, J. R.; FREIRE, T. C.; FONSECA, A. S. da; SANGI, S. C.; BARBIERI, F. da S.; ROCHA, R. B.; BRITO, L. G.; PEREIRA, S. dos S.; LUIZ, M. B.; FREIRE, F. das C. O.; FERNANDES, C. F. C.; SOARES, A. M.; FERNANDES, C. de F. Snake venoms and purified toxins as biotechnological tools to control Ralstonia solanacearum. Pesquisa Agropecuária Brasileira, Brasília, v.55, e01756, 2020. Título em português: Venenos e toxinas ofídicas purificadas como ferramenta biotecnológica para o controle de Ralstonia solanacearum.| Biblioteca(s): Embrapa Agroindústria Tropical; Embrapa Amazônia Oriental; Embrapa Rondônia; Embrapa Unidades Centrais. |
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| 105. | | BRITO, L. G.; BARBIERI, F. da S.; NERY, L. de O.; FUNNES-HUACCA, M. E.; MATTOS, P. S. R. de; SOARES FILHO, P. M. S.; SILVA, R. R. da; PELLEGRIN, A. O.; JULIANO, R. S.; ZIMMERMANN, N. P.; FIGUEIRO, M. R. Avaliação da taxa de infecção por Brucella spp. na interface bubalinos e animais silvestres na Amazônia. Porto Velho, RO: Embrapa Rondônia, 2017 8 p. (Embrapa Rondônia. Comunicado Técnico, 404). Pesquisadores da Embrapa Rondônia: Luciana Gatto Brito e Fábio da Silva Barbieri.| Biblioteca(s): Embrapa Rondônia. |
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| 106. | | BRITO, L. G.; BARBIERI, F. da S.; NERY, L. de O.; FUNNES-HUACCA, M. E.; MATTOS, P. S. R. de; SOARES FILHO, P. M. S.; SILVA, R. R. da; PELLEGRIN, A. O.; JULIANO, R. S.; ZIMMERMANN, N. P.; FIGUEIRO, M. R. Avaliação da taxa de infecção por Brucella spp. na interface bubalinos e animais silvestres na Amazônia. Porto Velho, RO: Embrapa Rondônia, 2017. 8 p. (Embrapa Rondônia. Comunicado Técnico, 404).| Biblioteca(s): Embrapa Pantanal; Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 106 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Pecuária Sudeste. |
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Data corrente: |
04/09/2017 |
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Data da última atualização: |
16/01/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
BRITO, L. G.; NERY, L. de O.; BARBIERI, F. da S.; HUACCA, M. E. F.; PEREIRA, S. dos S.; SILVA, R. R. da; FERNANDES, C. F. C.; OLIVEIRA, M. C. de S. |
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Afiliação: |
LUCIANA GATTO BRITO, CPATU; Louí de Oliveira Néry, Bolsista CPATU; FABIO DA SILVA BARBIERI, CPATU; Maribel Elizabeth Funes Huacca, UNIR; Soraya dos Santos Pereira, Fiocruz Rondônia; RENATA REIS DA SILVA, CPATU; Carla Freire Celedonio Fernandes, Fiocruz Rondônia; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE. |
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Título: |
Molecular quantitative assay for esterase-mediated organophosphate resistance in Rhipicephalus microplus. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Ticks and Tick-borne Diseases, v. 8, n. 5, p. 725-732, 2017. |
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DOI: |
https://doi.org/10.1016/j.ttbdis.2017.05.006 |
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Idioma: |
Inglês |
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Conteúdo: |
The use of pesticides is the main tool to control infestations of the cattle tick Rhipicephalus microplus, and organophosphate (OP) is one of the most used compounds for this purpose. Carboxylesterases (ChEs) are targets for OP pesticides in arthropods, and acetylcholinesterase 2 (AChE2) and esterase 1 (EST1) are metabolic enzymes involved in the xenobiotic detoxification process. The increase in the synthesis of these enzymes can be detected by the quantitative polymerase chain reaction (qPCR) assay, which was used to identify cattle tick populations resistant to OP pesticides. For that, two field populations of R. microplus were used, one previously identified by the larval packet test (LPT) as OP −sensitive (LC50 = 0.13 μg/cm2) and the other OP-resistant (LC50 = 8.14 μg/cm2). To promote the OP enzyme detoxification, groups of 10 females of the resistant strain were immersed in solutions of diazinon in technical grade at concentrations of 1.0 mg/ml, 2.5 mg/ml, and 5.0 mg/ml. The ticks that survived diazinon exposure were submitted to qPCR assay, which enabled observing an increase in AChE2 and EST1 synthesis in the OP-resistant strain when compared to the susceptible strain. The initial results of expression analysis suggest that the qPCR assay can discriminate OP-resistant and susceptible populations. The development and improvement of molecular diagnostic tests to identify pesticide resistant R. microplus populations are priorities and in the near future it will be important to expand the molecular targets involved in OP resistance, which could be used for better selection of effective strategies to control cattle tick populations. MenosThe use of pesticides is the main tool to control infestations of the cattle tick Rhipicephalus microplus, and organophosphate (OP) is one of the most used compounds for this purpose. Carboxylesterases (ChEs) are targets for OP pesticides in arthropods, and acetylcholinesterase 2 (AChE2) and esterase 1 (EST1) are metabolic enzymes involved in the xenobiotic detoxification process. The increase in the synthesis of these enzymes can be detected by the quantitative polymerase chain reaction (qPCR) assay, which was used to identify cattle tick populations resistant to OP pesticides. For that, two field populations of R. microplus were used, one previously identified by the larval packet test (LPT) as OP −sensitive (LC50 = 0.13 μg/cm2) and the other OP-resistant (LC50 = 8.14 μg/cm2). To promote the OP enzyme detoxification, groups of 10 females of the resistant strain were immersed in solutions of diazinon in technical grade at concentrations of 1.0 mg/ml, 2.5 mg/ml, and 5.0 mg/ml. The ticks that survived diazinon exposure were submitted to qPCR assay, which enabled observing an increase in AChE2 and EST1 synthesis in the OP-resistant strain when compared to the susceptible strain. The initial results of expression analysis suggest that the qPCR assay can discriminate OP-resistant and susceptible populations. The development and improvement of molecular diagnostic tests to identify pesticide resistant R. microplus populations are priorities and in the near future ... Mostrar Tudo |
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Palavras-Chave: |
Cattle tick; Organophosphate resistance. |
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Thesaurus NAL: |
esterases. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02493naa a2200253 a 4500
001 2076221
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008 2017 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.ttbdis.2017.05.006$2DOI
100 1 $aBRITO, L. G.
245 $aMolecular quantitative assay for esterase-mediated organophosphate resistance in Rhipicephalus microplus.$h[electronic resource]
260 $c2017
520 $aThe use of pesticides is the main tool to control infestations of the cattle tick Rhipicephalus microplus, and organophosphate (OP) is one of the most used compounds for this purpose. Carboxylesterases (ChEs) are targets for OP pesticides in arthropods, and acetylcholinesterase 2 (AChE2) and esterase 1 (EST1) are metabolic enzymes involved in the xenobiotic detoxification process. The increase in the synthesis of these enzymes can be detected by the quantitative polymerase chain reaction (qPCR) assay, which was used to identify cattle tick populations resistant to OP pesticides. For that, two field populations of R. microplus were used, one previously identified by the larval packet test (LPT) as OP −sensitive (LC50 = 0.13 μg/cm2) and the other OP-resistant (LC50 = 8.14 μg/cm2). To promote the OP enzyme detoxification, groups of 10 females of the resistant strain were immersed in solutions of diazinon in technical grade at concentrations of 1.0 mg/ml, 2.5 mg/ml, and 5.0 mg/ml. The ticks that survived diazinon exposure were submitted to qPCR assay, which enabled observing an increase in AChE2 and EST1 synthesis in the OP-resistant strain when compared to the susceptible strain. The initial results of expression analysis suggest that the qPCR assay can discriminate OP-resistant and susceptible populations. The development and improvement of molecular diagnostic tests to identify pesticide resistant R. microplus populations are priorities and in the near future it will be important to expand the molecular targets involved in OP resistance, which could be used for better selection of effective strategies to control cattle tick populations.
650 $aesterases
653 $aCattle tick
653 $aOrganophosphate resistance
700 1 $aNERY, L. de O.
700 1 $aBARBIERI, F. da S.
700 1 $aHUACCA, M. E. F.
700 1 $aPEREIRA, S. dos S.
700 1 $aSILVA, R. R. da
700 1 $aFERNANDES, C. F. C.
700 1 $aOLIVEIRA, M. C. de S.
773 $tTicks and Tick-borne Diseases$gv. 8, n. 5, p. 725-732, 2017.
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Embrapa Amazônia Oriental (CPATU) |
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