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| Registros recuperados : 55 | |
| 4. |  | MARTINS, C. F.; BÁO, S. N.; DODE, M. N.; RUMPF, R. Avaliação ultra-estrutural e da fragmentação do dna de espermatozóides bovinos conservados por diferentes tratamentos de liofilização. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 10., 2005, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2005. p. 91.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | MARTINS, C. F.; BÁO, S. N.; DODE, M. N.; CORREA, G. A.; RUMF, R. Effects of freeze-drying on cytology, ultrastructure, DNA fragmentation, and fertilizing ability of bovine sperm. Theriogenology, v. 67, p. 1307-1315, 2007.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | MARTINS, C. F.; DODE, M. N.; BÁO, S. N.; RUMPF, R. Utilização do teste com acridina laranja e túnel para avaliar a integridade dos espermatozóides liofilizados de bovinos. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 11., 2006, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2006. p. 110.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | ARDISSON-ARAÚJO, D. M. P.; MELO, F. L.; SIHLER, W.; RIBEIRO, B. M.; BÁO, S. N.; SOUZA, M. L. de. Genome organization of Erinnyis ello granulovirus (EeGV), an efficient biopesticide used in Brazilian cassava crops. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 13., 2013, Bonito, MS. Faça bonito: use controle biológico: anais. Brasília, DF: Embrapa, 2013.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 15. | | CUNHA, E. R. da; MARTINS, C. F.; SILVA, G. C.; CUMPA, H. C. B.; BAO, S. N. Effects of prolonged in vitro culture and cryopreservation on viability, DNA Fragmentation, chromosome stability and ultrastructure of bovine cells from amniotic fluid and umbilical cord. Reproduction in Domestic Animals, v. 49, n. 5, p. 806-812, Oct. 2014.| Biblioteca(s): Embrapa Cerrados. |
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| 16. | | SILVA, C. G.; CUNHA, E. R.; BLUME, G. R.; MALAQUIAS, J. V.; BÁO, S. N.; MARTINS, C. F. Cryopreservation of boar sperm comparing different cryoprotectants associated in media based on powdered coconut water, lactose and trehalose. Cryobiology, v. 70, p. 90-94, 2015. p. 90-94| Biblioteca(s): Embrapa Cerrados. |
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| 18. | | BAPTISTELLA, J. C.; SILVA, C. G. da; BÁO, S. N.; PANEGOSSI, L. C.; CARDOSO, T. C.; CARVALHO, R. G. de; MARTINS, C. F. Immunomodulatory-associated gene transcripts to multipotency of bovine amniotic fluid mesenchymal stem cells. Animal Reproduction, v. 21, n. 1, e20230155, 2024.| Biblioteca(s): Embrapa Cerrados. |
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| 19. | | JOVITO, B. L.; PATERNO, L. G.; SALES, M. J. A.; GROSS, M. A.; SILVA, L. P. da; SOUZA, P. de; BÁO, S. N. Graphene oxide/zinc oxide nanocomposite displaying selective toxicity to glioblastoma cell lines. ACS Applied Bio Materials, v. 4, p. 829-843, 2021. Na publicação: Luciano P. Silva.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 20. | | PARENTE, A. F.; BALDANI, J. I.; PEREIRA, I. S.; TIBÚRCIO, V. H. da S.; BÁO, S. N.; DE SOUZA, M. T. GFP expression in wild-type B. thuringiensis strain active to Lepidoptera. In: MEETING OF THE SOCIETY FOR INVERTEBRATE PATHOLOGY, 40th., INTERNATIONAL FORUM ON ENTOMOPATHOGENIC NEMATODES AND SYMBIOTIC BACTERIA, 1st., 2007, Quebec City. Anais... Quebec: Society for Invertebrate Pathology, 2007. p. 59. Parceria: UnB.| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 55 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
11/12/2020 |
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Data da última atualização: |
11/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
ARANTES, L. G.; TONELLI, G. S. S. S.; MARTINS, C. F.; BÁO, S. N. |
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Afiliação: |
CARLOS FREDERICO MARTINS, CPAC. |
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Título: |
Cellular Characterization and Effectsof Cryoprotectant Solutions on the Viabilityof Fibroblasts from Three Brazilian Wild Cats. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Biopreservation and Biobanking, 2020. |
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Idioma: |
Português |
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Conteúdo: |
Preserving genetic material in cryogenic conditions presents a viable alternative for the protection of species' gene variability. However, there is an enormous need to establish and test cryopreservation protocols that are suitable for each diverse cell type to guarantee technical success in the long run. Considering this, fibroblasts from jaguar (Panthera onca), oncilla (Leopardus tigrinus), and pampas cat (Leopardus colocolo) were subjected to cell characterization and then cryopreservation in different cryoprotectant solutions (2.5%, 10% dimethyl sulfoxide [DMSO] or CryoSOfree?). Further testing was conducted to determine each solution's performance in preserving cell viability. In culture, a growth curve to assess cellular growth potential showed that exponential proliferation lasts for about the first 50 hours of in vitro culturing, declining in pace afterward. L. colocolo and L. tigrinus presented no difference in cell viability while using 2.5% DMSO protocols. P. onca cells did not present difference on viability for both concentrations of DMSO. Protocols using CryoSOfree resulted in a decreased viability of P. onca fibroblasts. Morphological differences between fibroblasts among the species were noted under bright field microscopy and scanning electron microscopy. L. colocolo and P. onca cells are fusiform, and L. tigrinus are spherical. All cells presented cytoplasmic projections. Transmission electron microscopy revealed vacuoles and secretion granules, indicating intense cell activity after thawing. Differences found in the efficiency of cryopreservation protocols according to the type of cryoprotectant indicate that species react differently to freezing and thawing processes. This research evaluates key aspects of in vitro protocols for cryopreservation of wild animals, which need to be optimized to guarantee successful cell culturing. More suitable protocols lead to increased efficiency in establishing fibroblast cryobanks and also facilitating the use of wild cats' cells in cloning techniques, contributing directly to preserving wild fauna. MenosPreserving genetic material in cryogenic conditions presents a viable alternative for the protection of species' gene variability. However, there is an enormous need to establish and test cryopreservation protocols that are suitable for each diverse cell type to guarantee technical success in the long run. Considering this, fibroblasts from jaguar (Panthera onca), oncilla (Leopardus tigrinus), and pampas cat (Leopardus colocolo) were subjected to cell characterization and then cryopreservation in different cryoprotectant solutions (2.5%, 10% dimethyl sulfoxide [DMSO] or CryoSOfree?). Further testing was conducted to determine each solution's performance in preserving cell viability. In culture, a growth curve to assess cellular growth potential showed that exponential proliferation lasts for about the first 50 hours of in vitro culturing, declining in pace afterward. L. colocolo and L. tigrinus presented no difference in cell viability while using 2.5% DMSO protocols. P. onca cells did not present difference on viability for both concentrations of DMSO. Protocols using CryoSOfree resulted in a decreased viability of P. onca fibroblasts. Morphological differences between fibroblasts among the species were noted under bright field microscopy and scanning electron microscopy. L. colocolo and P. onca cells are fusiform, and L. tigrinus are spherical. All cells presented cytoplasmic projections. Transmission electron microscopy revealed vacuoles and secretion granules, indicating... Mostrar Tudo |
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Palavras-Chave: |
Material genético; Protocolo. |
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Thesagro: |
Criopreservação. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02694naa a2200193 a 4500
001 2128006
005 2020-12-11
008 2020 bl uuuu u00u1 u #d
100 1 $aARANTES, L. G.
245 $aCellular Characterization and Effectsof Cryoprotectant Solutions on the Viabilityof Fibroblasts from Three Brazilian Wild Cats.$h[electronic resource]
260 $c2020
520 $aPreserving genetic material in cryogenic conditions presents a viable alternative for the protection of species' gene variability. However, there is an enormous need to establish and test cryopreservation protocols that are suitable for each diverse cell type to guarantee technical success in the long run. Considering this, fibroblasts from jaguar (Panthera onca), oncilla (Leopardus tigrinus), and pampas cat (Leopardus colocolo) were subjected to cell characterization and then cryopreservation in different cryoprotectant solutions (2.5%, 10% dimethyl sulfoxide [DMSO] or CryoSOfree?). Further testing was conducted to determine each solution's performance in preserving cell viability. In culture, a growth curve to assess cellular growth potential showed that exponential proliferation lasts for about the first 50 hours of in vitro culturing, declining in pace afterward. L. colocolo and L. tigrinus presented no difference in cell viability while using 2.5% DMSO protocols. P. onca cells did not present difference on viability for both concentrations of DMSO. Protocols using CryoSOfree resulted in a decreased viability of P. onca fibroblasts. Morphological differences between fibroblasts among the species were noted under bright field microscopy and scanning electron microscopy. L. colocolo and P. onca cells are fusiform, and L. tigrinus are spherical. All cells presented cytoplasmic projections. Transmission electron microscopy revealed vacuoles and secretion granules, indicating intense cell activity after thawing. Differences found in the efficiency of cryopreservation protocols according to the type of cryoprotectant indicate that species react differently to freezing and thawing processes. This research evaluates key aspects of in vitro protocols for cryopreservation of wild animals, which need to be optimized to guarantee successful cell culturing. More suitable protocols lead to increased efficiency in establishing fibroblast cryobanks and also facilitating the use of wild cats' cells in cloning techniques, contributing directly to preserving wild fauna.
650 $aCriopreservação
653 $aMaterial genético
653 $aProtocolo
700 1 $aTONELLI, G. S. S. S.
700 1 $aMARTINS, C. F.
700 1 $aBÁO, S. N.
773 $tBiopreservation and Biobanking, 2020.
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