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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
04/06/1998 |
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Data da última atualização: |
15/12/2020 |
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Autoria: |
FAULL, J. L.; MELO, I. S. de; GRAEME-COOK, K. A. |
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Afiliação: |
EMBRAPA-CNPMA; ITAMAR SOARES DE MELO, CNPMA. |
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Título: |
Tracking extra-celullar enzyme production by Trichoderma harzianum in the rhizosphere. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 6., 1998, Rio de Janeiro. Anais: conferências e mesas-redondas. Rio de Janeiro: FIOCRUZ/EMBRAPA, 1998. p.361. |
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Idioma: |
Inglês |
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Conteúdo: |
The exact role of extracellular enzyme production in the activities of Trichoderma harzianum as a biological control agent are much debated. Production of such enzymes as cellulase, proteinase, endoglucanase, (glucosidase and chitinase has been linked to biological control in vitro experiments (Elad et al, 1982, Ahmad & Baker, 1988 a,b) bur it has been difficult to prove that these enzymes are important in vivo. Baker (1991) demonstrated that mutant strains of Trichoderma with improved rhizosphere competence had higher extracellular cellulase production than wild types strains, and he speculated that enhanced cellulase production might lead to better utilisation of rhizosphere substrates and thus improved colonisation of the resource. However, it has been recently shown that rhizosphere competence of Trichoderma strains is not always associated with improved in vitro production of extracellular enzymes (de Melo, Faull, J, L, and Graeme-Cook, 1997). In order to extend this work to the soil environment we have developed a sensitive, quantifiable assay for extracellular enzymes that uses a fluorescent substrate to detect enzyme activity in soil. Using a number of different strains of Trichoderma with known in vitro extra-cellular enzyme production patterns (both high and low) we will demonstrate differences in the production of enzymes in the rhizosphere with varying soil depth. We will attempt to relate these differences to the root colonisation ability of the Trichoderma strain. MenosThe exact role of extracellular enzyme production in the activities of Trichoderma harzianum as a biological control agent are much debated. Production of such enzymes as cellulase, proteinase, endoglucanase, (glucosidase and chitinase has been linked to biological control in vitro experiments (Elad et al, 1982, Ahmad & Baker, 1988 a,b) bur it has been difficult to prove that these enzymes are important in vivo. Baker (1991) demonstrated that mutant strains of Trichoderma with improved rhizosphere competence had higher extracellular cellulase production than wild types strains, and he speculated that enhanced cellulase production might lead to better utilisation of rhizosphere substrates and thus improved colonisation of the resource. However, it has been recently shown that rhizosphere competence of Trichoderma strains is not always associated with improved in vitro production of extracellular enzymes (de Melo, Faull, J, L, and Graeme-Cook, 1997). In order to extend this work to the soil environment we have developed a sensitive, quantifiable assay for extracellular enzymes that uses a fluorescent substrate to detect enzyme activity in soil. Using a number of different strains of Trichoderma with known in vitro extra-cellular enzyme production patterns (both high and low) we will demonstrate differences in the production of enzymes in the rhizosphere with varying soil depth. We will attempt to relate these differences to the root colonisati... Mostrar Tudo |
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Thesagro: |
Controle Biológico; Enzima; Trichoderma. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/205585/1/Faull-tracking.pdf
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Marc: |
LEADER 02135naa a2200181 a 4500
001 1012921
005 2020-12-15
008 1998 bl uuuu u00u1 u #d
100 1 $aFAULL, J. L.
245 $aTracking extra-celullar enzyme production by Trichoderma harzianum in the rhizosphere.$h[electronic resource]
260 $c1998
520 $aThe exact role of extracellular enzyme production in the activities of Trichoderma harzianum as a biological control agent are much debated. Production of such enzymes as cellulase, proteinase, endoglucanase, (glucosidase and chitinase has been linked to biological control in vitro experiments (Elad et al, 1982, Ahmad & Baker, 1988 a,b) bur it has been difficult to prove that these enzymes are important in vivo. Baker (1991) demonstrated that mutant strains of Trichoderma with improved rhizosphere competence had higher extracellular cellulase production than wild types strains, and he speculated that enhanced cellulase production might lead to better utilisation of rhizosphere substrates and thus improved colonisation of the resource. However, it has been recently shown that rhizosphere competence of Trichoderma strains is not always associated with improved in vitro production of extracellular enzymes (de Melo, Faull, J, L, and Graeme-Cook, 1997). In order to extend this work to the soil environment we have developed a sensitive, quantifiable assay for extracellular enzymes that uses a fluorescent substrate to detect enzyme activity in soil. Using a number of different strains of Trichoderma with known in vitro extra-cellular enzyme production patterns (both high and low) we will demonstrate differences in the production of enzymes in the rhizosphere with varying soil depth. We will attempt to relate these differences to the root colonisation ability of the Trichoderma strain.
650 $aControle Biológico
650 $aEnzima
650 $aTrichoderma
700 1 $aMELO, I. S. de
700 1 $aGRAEME-COOK, K. A.
773 $tIn: SIMPÓSIO DE CONTROLE BIOLÓGICO, 6., 1998, Rio de Janeiro. Anais: conferências e mesas-redondas. Rio de Janeiro: FIOCRUZ/EMBRAPA, 1998. p.361.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio-Norte. Para informações adicionais entre em contato com cpamn.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
07/08/2009 |
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Data da última atualização: |
07/08/2009 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
MELO, F. de B.; BALSADI, O. V. |
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Afiliação: |
Francisco de Brito Melo, Embrapa Meio-Norte; Otavio Valentim Balsadi, Secretaria de Gestão e Estratégia da Embrapa. |
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Título: |
Inclusão social na produção familiar de biodiesel: energia, renda e cidadania no Semi-Árido piauiense. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In: SOUSA, I. S. F. de; CABRAL, J. R. F. (Ed.). Ciência como instrumento de inclusão social. Brasília, DF: Embrapa Informação Tecnológica, 2009. |
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Páginas: |
p. 177-208 |
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Idioma: |
Português |
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Thesagro: |
Agricultura Familiar; Sistema de Produção. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00597naa a2200157 a 4500
001 1256516
005 2009-08-07
008 2009 bl uuuu u00u1 u #d
100 1 $aMELO, F. de B.
245 $aInclusão social na produção familiar de biodiesel$benergia, renda e cidadania no Semi-Árido piauiense.
260 $c2009
300 $ap. 177-208
650 $aAgricultura Familiar
650 $aSistema de Produção
700 1 $aBALSADI, O. V.
773 $tIn: SOUSA, I. S. F. de; CABRAL, J. R. F. (Ed.). Ciência como instrumento de inclusão social. Brasília, DF: Embrapa Informação Tecnológica, 2009.
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