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| Registros recuperados : 231 | |
| Registros recuperados : 231 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
19/06/2019 |
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Data da última atualização: |
13/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
BOTTA, D.; ARRUDA, R. P. de; WATANABE, Y. F.; BALIEIRO, J. C. de C.; ROMANELLO, N.; BARRETO, A. do N.; PANTOJA, M. H. de A.; GIRO, A.; CARVALHO, C. P. T. de; OLIVEIRA, A. de S.; GARCIA, A. R. |
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Afiliação: |
Daniela Botta, UFPA; Rubens Paes de Arruda, USP; Yeda Fumie Watanabe, Vitrogen - YVF Biotech; Júlio Cesar de Carvalho Balieiro, USP; Narian Romanello, UFPA; Andréa do Nascimento Barreto, UFPA; Messy Hannear de Andrade Pantoja, UFPA; Alessandro Giro, UFPA; Carla Patrícia Teodoro de Carvalho, USP; Aline de Sousa Oliveira, Vitrogen - YVF Biotech; ALEXANDRE ROSSETTO GARCIA, CPPSE. |
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Título: |
Influence of post-thawing thermal environment on bovine sperm characteristics and in vitro fertility. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Andrologia, v. 51, n. 6, e13266, jul. 2019. |
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DOI: |
10.1111/and.13266 |
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Idioma: |
Inglês |
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Conteúdo: |
Our aim was to evaluate the effects of three thermal environments over time on kinetics, functionality and in vitro fertility of cryopreserved bovine spermatozoa. Four ejaculates from five bulls (n = 20) were cryopreserved. After thawing, semen was evaluated (0 hr), incubated for 4 hr in T36.0 (36.0°C), T38.0 (38.0°C) and T39.5 (39.5°C), and analysed every hour (1 hr, 2 hr, 3 hr, 4 hr). In vitro production of embryos was performed at 0 hr and 4 hr. Sperm motility and cell kinetics (Computer Assisted Sperm Analysis) were impaired after 2 hr at T38.0 and T39.5 (p < 0.05). Flow cytometry revealed an increase in the cells with injured plasma membrane to 39.5°C and a general reduction in the mitochondrial potential over time (p < 0.05). In vitro fertility was impaired in all temperatures after 4 hr, but there was no difference between 36.0°C and 38.0°C. Our results suggest that the ex situ resilience of semen at 36.0°C after thawing with no major damage to the quality is limited to 3 hr. In normothermia or in thermal stress, sperm cells present a gradual reduction of movement and functionality, which were more significant after 1 hr of incubation. The in vitro production of embryos is impaired when the semen is kept in a thermal environment ≥36.0°C for 4 hr. |
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Palavras-Chave: |
Semen quality; Thermotolerance. |
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Thesagro: |
Inseminação Artificial; Touro. |
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Thesaurus NAL: |
Bulls; Heat stress; In vitro fertilization. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/198716/1/BOTTA-et-al-2019-Influence-of-Post-thawing-Thermal-Environment-on-Bovine-Sperm-Characteristics.pdf
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Marc: |
LEADER 02263naa a2200337 a 4500
001 2109972
005 2023-03-13
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1111/and.13266$2DOI
100 1 $aBOTTA, D.
245 $aInfluence of post-thawing thermal environment on bovine sperm characteristics and in vitro fertility.$h[electronic resource]
260 $c2019
520 $aOur aim was to evaluate the effects of three thermal environments over time on kinetics, functionality and in vitro fertility of cryopreserved bovine spermatozoa. Four ejaculates from five bulls (n = 20) were cryopreserved. After thawing, semen was evaluated (0 hr), incubated for 4 hr in T36.0 (36.0°C), T38.0 (38.0°C) and T39.5 (39.5°C), and analysed every hour (1 hr, 2 hr, 3 hr, 4 hr). In vitro production of embryos was performed at 0 hr and 4 hr. Sperm motility and cell kinetics (Computer Assisted Sperm Analysis) were impaired after 2 hr at T38.0 and T39.5 (p < 0.05). Flow cytometry revealed an increase in the cells with injured plasma membrane to 39.5°C and a general reduction in the mitochondrial potential over time (p < 0.05). In vitro fertility was impaired in all temperatures after 4 hr, but there was no difference between 36.0°C and 38.0°C. Our results suggest that the ex situ resilience of semen at 36.0°C after thawing with no major damage to the quality is limited to 3 hr. In normothermia or in thermal stress, sperm cells present a gradual reduction of movement and functionality, which were more significant after 1 hr of incubation. The in vitro production of embryos is impaired when the semen is kept in a thermal environment ≥36.0°C for 4 hr.
650 $aBulls
650 $aHeat stress
650 $aIn vitro fertilization
650 $aInseminação Artificial
650 $aTouro
653 $aSemen quality
653 $aThermotolerance
700 1 $aARRUDA, R. P. de
700 1 $aWATANABE, Y. F.
700 1 $aBALIEIRO, J. C. de C.
700 1 $aROMANELLO, N.
700 1 $aBARRETO, A. do N.
700 1 $aPANTOJA, M. H. de A.
700 1 $aGIRO, A.
700 1 $aCARVALHO, C. P. T. de
700 1 $aOLIVEIRA, A. de S.
700 1 $aGARCIA, A. R.
773 $tAndrologia$gv. 51, n. 6, e13266, jul. 2019.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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