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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
26/01/2024 |
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Data da última atualização: |
26/01/2024 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
CASTRO, C. de; OLIVEIRA, F. A. de; FIRMANO, R. F.; OLIVEIRA JUNIOR, A. de. |
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Afiliação: |
CESAR DE CASTRO, CNPSO; FABIO ALVARES DE OLIVEIRA, CNPSO; RUAN FRANCISCO FIRMANO, Conselho Nacional de Desenvolvimento Científico e Tecnológico.; ADILSON DE OLIVEIRA JUNIOR, CNPSO. |
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Título: |
AFERE: soil fertility assessment and fertilizer recommendation webtool to agricultural systems. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 11., 2023, Vienna. Soybean Research for Sustainable Development. abstracts. Vienna: University of Natural Resources and Life Sciences, 2023. |
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Páginas: |
p. 408 |
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Idioma: |
Português |
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Notas: |
WSRC |
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Thesagro: |
Fertilidade do Solo; Fertilizante; Soja. |
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Thesaurus Nal: |
Fertilizers; Soil fertility; Soybeans. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 00801nam a2200229 a 4500
001 2161388
005 2024-01-26
008 2023 bl uuuu u00u1 u #d
100 1 $aCASTRO, C. de
245 $aAFERE$bsoil fertility assessment and fertilizer recommendation webtool to agricultural systems.$h[electronic resource]
260 $aIn: WORLD SOYBEAN RESEARCH CONFERENCE, 11., 2023, Vienna. Soybean Research for Sustainable Development. abstracts. Vienna: University of Natural Resources and Life Sciences$c2023
300 $ap. 408
500 $aWSRC
650 $aFertilizers
650 $aSoil fertility
650 $aSoybeans
650 $aFertilidade do Solo
650 $aFertilizante
650 $aSoja
700 1 $aOLIVEIRA, F. A. de
700 1 $aFIRMANO, R. F.
700 1 $aOLIVEIRA JUNIOR, A. de
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Registro original: |
Embrapa Soja (CNPSO) |
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Biblioteca |
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Cutter |
Registro |
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URL |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
14/02/2011 |
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Data da última atualização: |
19/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
CHIARATTI, M. R.; FERREIRA, C. R.; PERECIN, F.; NICIURA, S. C. M.; SANGALLI, J. R.; MESQUITA, L. G.; BALIEIRO, J. C. de C.; SMITH, L. C.; GARCIA, J. M.; MEIRELLES, F. V. |
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Afiliação: |
MARCOS ROBERTO CHIARATTI, USP/PIRASSUNGA; CHRISTINA RAMIRES FERREIRA, USP/PIRASSUNGA; FELIPE PERECIN, USP/PIRASSUNUNGA; SIMONE CRISTINA MEO NICIURA, CPPSE; JULIANO RODRIGUES SANGALLI, USP/PIRASSUNUGA; LÍGIA GARCIA MESQUITA, USP/PIRASSUNGA; JÚLIO CÉSAR DE CARVALHO BALIEIRO, USP/PIRASSUNUNGA; LAWRENCE CHARLES SMITH, UNIVERSITE DE MONTREAL/SAINT-HYACINTHE; JOAQUIM MANSANO GARCIA, UNESP/JABOTICABAL; FLÁVIO VIEIRA MEIRELLES, USP/PIRASSUNUNGA. |
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Título: |
Ooplast-mediated developmental rescue of bovine oocytes exposed to ethidium bromide. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Reproductive BioMedicine Online v. 22, n. 22, p. 172-183, 2011. |
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DOI: |
https://doi.org/10.1016/j.rbmo.2010.10.011 |
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Idioma: |
Inglês |
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Conteúdo: |
Ooplasm transfer has been used successfully to treat infertility in women with ooplasmic insufficiency and has culminated in the birth of healthy babies. To investigate whether mitochondrial dysfunction is a factor in ooplasmic insufficiency, bovine oocytes were exposed to ethidium bromide, an inhibitor of mitochondrial DNA replication and transcription, during in-vitro maturation (IVM). Exposure of immature oocytes to ethidium bromide for 24?h during IVM hampered meiotic resumption and the migration of cortical granules. However, a briefer treatment with ethidium bromide during the last 4?h of IVM led to partial arrest of preimplantation development without affecting oocyte maturation. Ooplasm transfer was then performed to rescue the oocytes with impaired development. In spite of this developmental hindrance, transfer of normal ooplasm into ethidium bromide-treated oocytes resulted in a complete rescue of embryonic development and the birth of heteroplasmic calves. Although this study unable to determine whether developmental rescue occurred exclusively through introduction of unaffected mitochondria into ethidium bromide-damaged oocytes, e.g. ethidium bromide may also affect other ooplasm components, these results clearly demonstrate that ooplasm transfer can completely rescue developmentally compromised oocytes, supporting the potential use of ooplasm transfer in therapeutic applications. Although the transfer of egg cytoplasm between women has been used successfully to rescue development in infertile patients culminating in the birth of healthy babies, the aetiology of egg-related infertility remains poorly understood. To verify the role played by a dysfunction in the mitochondrial activity, bovine eggs were exposed to ethidium bromide, a drug commonly used to impair mitochondrial function. We show that eggs exposed to ethidium bromide arrest embryonic development after IVF. Moreover, a complete rescue of development was observed after transfer of cytoplasm from untreated eggs into ethidium bromide-treated eggs leading to the birth of heteroplasmic calves which contained mitochondria from donor and recipient eggs (heteroplasmy). Although we were unable to confirm that mitochondria were the only ooplasm component affected by ethidium bromide, we provide evidence that cytoplasm transfer can completely rescue developmentally compromised oocytes. MenosOoplasm transfer has been used successfully to treat infertility in women with ooplasmic insufficiency and has culminated in the birth of healthy babies. To investigate whether mitochondrial dysfunction is a factor in ooplasmic insufficiency, bovine oocytes were exposed to ethidium bromide, an inhibitor of mitochondrial DNA replication and transcription, during in-vitro maturation (IVM). Exposure of immature oocytes to ethidium bromide for 24?h during IVM hampered meiotic resumption and the migration of cortical granules. However, a briefer treatment with ethidium bromide during the last 4?h of IVM led to partial arrest of preimplantation development without affecting oocyte maturation. Ooplasm transfer was then performed to rescue the oocytes with impaired development. In spite of this developmental hindrance, transfer of normal ooplasm into ethidium bromide-treated oocytes resulted in a complete rescue of embryonic development and the birth of heteroplasmic calves. Although this study unable to determine whether developmental rescue occurred exclusively through introduction of unaffected mitochondria into ethidium bromide-damaged oocytes, e.g. ethidium bromide may also affect other ooplasm components, these results clearly demonstrate that ooplasm transfer can completely rescue developmentally compromised oocytes, supporting the potential use of ooplasm transfer in therapeutic applications. Although the transfer of egg cytoplasm between women has been used successfully to ... Mostrar Tudo |
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Palavras-Chave: |
Embryo; Ethidium bromide; Oocyte; Ooplasm; Transfer. |
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Thesaurus NAL: |
mitochondrial DNA. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/876985/1/OoplastMediatedDevelopmental.pdf
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Marc: |
LEADER 03301naa a2200313 a 4500
001 1876985
005 2022-07-19
008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.rbmo.2010.10.011$2DOI
100 1 $aCHIARATTI, M. R.
245 $aOoplast-mediated developmental rescue of bovine oocytes exposed to ethidium bromide.$h[electronic resource]
260 $c2011
520 $aOoplasm transfer has been used successfully to treat infertility in women with ooplasmic insufficiency and has culminated in the birth of healthy babies. To investigate whether mitochondrial dysfunction is a factor in ooplasmic insufficiency, bovine oocytes were exposed to ethidium bromide, an inhibitor of mitochondrial DNA replication and transcription, during in-vitro maturation (IVM). Exposure of immature oocytes to ethidium bromide for 24?h during IVM hampered meiotic resumption and the migration of cortical granules. However, a briefer treatment with ethidium bromide during the last 4?h of IVM led to partial arrest of preimplantation development without affecting oocyte maturation. Ooplasm transfer was then performed to rescue the oocytes with impaired development. In spite of this developmental hindrance, transfer of normal ooplasm into ethidium bromide-treated oocytes resulted in a complete rescue of embryonic development and the birth of heteroplasmic calves. Although this study unable to determine whether developmental rescue occurred exclusively through introduction of unaffected mitochondria into ethidium bromide-damaged oocytes, e.g. ethidium bromide may also affect other ooplasm components, these results clearly demonstrate that ooplasm transfer can completely rescue developmentally compromised oocytes, supporting the potential use of ooplasm transfer in therapeutic applications. Although the transfer of egg cytoplasm between women has been used successfully to rescue development in infertile patients culminating in the birth of healthy babies, the aetiology of egg-related infertility remains poorly understood. To verify the role played by a dysfunction in the mitochondrial activity, bovine eggs were exposed to ethidium bromide, a drug commonly used to impair mitochondrial function. We show that eggs exposed to ethidium bromide arrest embryonic development after IVF. Moreover, a complete rescue of development was observed after transfer of cytoplasm from untreated eggs into ethidium bromide-treated eggs leading to the birth of heteroplasmic calves which contained mitochondria from donor and recipient eggs (heteroplasmy). Although we were unable to confirm that mitochondria were the only ooplasm component affected by ethidium bromide, we provide evidence that cytoplasm transfer can completely rescue developmentally compromised oocytes.
650 $amitochondrial DNA
653 $aEmbryo
653 $aEthidium bromide
653 $aOocyte
653 $aOoplasm
653 $aTransfer
700 1 $aFERREIRA, C. R.
700 1 $aPERECIN, F.
700 1 $aNICIURA, S. C. M.
700 1 $aSANGALLI, J. R.
700 1 $aMESQUITA, L. G.
700 1 $aBALIEIRO, J. C. de C.
700 1 $aSMITH, L. C.
700 1 $aGARCIA, J. M.
700 1 $aMEIRELLES, F. V.
773 $tReproductive BioMedicine Online$gv. 22, n. 22, p. 172-183, 2011.
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