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| Registros recuperados : 43 | |
| 21. |  | AZEVEDO, D. A. A. de; ALVES, S. M.; DIAS, R. P.; FEITOSA, A. L. V. L.; PINHEIRO, R. R.; ANDRIOLI, A. Uso de co-cultivo de leucócitos no isolamento do vírus da artrite encefalite caprina em rebanhos de Fortaleza/CE. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA UNIVERSIDADE ESTADUAL VALE DO ACARAÚ, 7., 2010, Sobral. Iniciação para o semiárido: [Resumos]. Sobral: Universidade Estadual Vale do Acaraú, 2010. 1 CD ROM.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 22. | | LIMA, C. C. V. de; AYRES, M. C. C; PINHEIRO, R. R.; COSTA, J. N.; ANDRIOLI, A.; SOUZA, T. S. de; AZEVEDO, D. A. A. de; PEIXOTO, R. M.; DAMASCENO, E. M.; SILVA, J. B. R. da. Lentivírus caprino no sêmen de ovinos. Biológico, São Paulo, v. 77, p. 119, 2015. Suplemento 2, ref. 238. Edição dos Resumos XI Congresso Brasileiro de Buiatria, XVII Congresso Latinoamericano de Buiatria, jul. 2015.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 23. | | AZEVEDO, D. A. A. de; FEITOSA, A. L. V. L.; ALVES, S. M.; SOUZA, K. S.; SOUSA, T. B. C.; BEZERRA JÚNIOR, R. Q.; ANDRIOLI, A.; PINHEIRO, R. R.; TEIXEIRA, M. F. S. Isolation of caprine arthritis encephalitis virus from infected macrophage submitted to cultures. Virus Reviews and Research, v. 16, supl. 1, p. 102, oct., 2011. Abstract. BV63.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 24. | | SOUZA, T. S. de; PINHEIRO, R. R.; COSTA, J. N.; LIMA, C. C. V. de; ANDRIOLI, A.; AZEVEDO, D. A. A. de; SANTOS, V. W. S. dos; ARAÚJO, J. F.; SOUZA, A. L. M. de; COSTA NETO, A. O. Interspecies transmission of lentiviruses from goats to sheep. Biológico, São Paulo, v. 77, p. 119, 2015. Suplemento 2, ref. 237. Edição dos Resumos XI Congresso Brasileiro de Buiatria, XVII Congresso Latinoamericano de Buiatria, jul. São Paulo, 2015.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 25. | | PEIXOTO, R. M.; ANDRIOLI, A.; PINHEIRO, R. R.; ALVES, F. S. F.; SANTOS, V. W. S. dos; SOUSA, M. M. de; AZEVEDO, D. A. A. de; DAMASCENO, E. M.; TEIXEIRA, M. F. da S. Mycoplasma agalactiae em rebanhos leiteiros no estado do Ceará em associação com o vírus da artrite encefalite caprina. Acta Scientiae Veterinariae, v. 46, Pub. 1533, 2018.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 26. | | AZEVEDO, D. A. A. de; MONTEIRO, J. P.; PINHEIRO, R. R.; MUDADU, M. de A.; ANDRIOLI, A.; ARAÚJO, J. F.; SOUSA, A. L. M. de; SIDER, L. H.; PEIXOTO, R. M.; TEIXEIRA, M. F. da S. Molecular characterization of circulating strains of small ruminant lentiviruses in Brazil based on complete gag and pol genes. Small Ruminant Research, v. 177, p. 160-166, 2019.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Caprinos e Ovinos. |
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| 27. | | AZEVEDO, D. A. A. de; PINHEIRO, R. R.; SANTOS, V. W. S. dos; DAMASCENO, E. M.; SOUSA, A. L. M. de; ARAÚJO, J. F.; ANDRIOLI, A.; SIDER, L. H.; PEIXOTO, R. M.; TEIXEIRA, M. F. da S. Comparação de testes sorológicos e molecular para diagnóstico da Artrite Encefalite Caprina e avaliação clínica da glândula mamária de caprinos leiteiros infectados. Acta Scientiae Veterinariae, v. 47, Pub. 1668, 2019.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 28. | | AZEVEDO, D. A. A. de; ARAÚJO, J. F.; LIMA, C. C. V. de; SOUZA, T. S. de; SOUSA, A. L. M. de; ANDRIOLI, A.; CRUZ, J. C. M. da; PINHEIRO, R. R. Análise protéica e imunogênica de antígenos de lentivírus de pequenos ruminantes. In: CONGRESSO NORDESTINO DE PRODUÇÃO ANIMAL, 7.; SIMPÓSIO NORDESTINO DE ALIMENTAÇÃO DE RUMINANTES, 13., 2012, Maceió. Anais... Maceió: Sociedade Nordestina de Produção Animal, 2012. 3 f. 1 CD-ROM.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 29. | | ARAÚJO, J. F.; ANDRIOLI, A.; PINHEIRO, R. R.; PEIXOTO, R. M.; SOUSA, A. L. M. de; AZEVEDO, D. A. A. de; LIMA, A. M. C.; NOBRE, J. A.; AMARAL, G. P.; BRANDÃO, I. S.; TEIXEIRA, M. F. da S. Detection and isolation of small ruminant lentivirus in the amniotic fluid of goats. Comparative Immunology, Microbiology and Infectious Diseases, v. 78, 101693, Oct. 2021.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 30. | | LIMA, C. C. V. de; AYRES, M. C. C.; PINHEIRO, R. R.; COSTA, J. N.; COSTA NETO, A. O.; ANDRIOLI, A. A.; SOUZA, T. S. de; AZEVEDO, D. A. A. de; SANTOS, V. W. S. dos; OLIVEIRA, L. G. Influência da lentivirose no metabolismo de ovinos. Biológico, São Paulo, v. 77, p. 120, 2015. Suplemento 2, ref. 240 Edição dos Resumos XI Congresso Brasileiro de Buiatria, XVII Congresso Latinoamericano de Buiatria, jul. São Paulo, 2015.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 31. | | PINHEIRO, D. N. S.; COSTA, J. N.; SOUZA, T. S. de; LIMA, C. C. V. de; MACEDO, D. R.; CORREIA, G. S.; SANTOS, V. W. S. dos; AZEVEDO, D. A. A. de; CERQUEIRA, R. B.; PINHEIRO, R. R. Inquérito soro epidemiológico e fatores de risco para lentivírus em rebanhos caprinos leiteiros e de corte da região Sisaleira, Bahia. Biológico, São Paulo, v. 77, p. 158, 2015. Suplemento 2, ref. 315. Edição dos Resumos XI Congresso Brasileiro de Buiatria, XVII Congresso Latinoamericano de Buiatria, jul. São Paulo, 2015.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 32. | | SOUZA, T. S. de; PINHEIRO, R. R.; LIMA, C. C. V. de; ANDRIOLI, A.; AZEVEDO, D. A. A. de; SANTOS, W. W. S. dos; SOUZA, A. L. M. de; ARAÚJO, J. F.; PINHEIRO, D. N. dos S.; COSTA, J. N. Infecção de cordeiros por lentivírus caprino. In: CONGRESSO BRASILEIRO DE BUIATRIA, 10.; SEMANA DO MÉDICO VETERINÁRIO DO PARÁ, 37.; SIMPÓSIO PARAENSE DE MEDICINA VETERINÁRIA, 5., 2013, Belém, PA. Anais... Belém, PA: Associação Brasileira de Buiatria, 2013. 1 CD-ROM.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 33. | | PEIXOTO, R. M.; TEIXEIRA, M. F. da S.; PINHEIRO, A. A.; PINHEIRO, R. R.; SILVA, A. A. de S.; AGUIAR, T. D. de F.; AZEVEDO, D. A. A. de; SOUSA, A. L. M. de. Perspectivas do uso de fitoterápicos no tratamento do sêmen caprino infectado pelo vírus da Artrite Encefalite Caprina. Medicina Veterinária (UFRPE), v. 12, n. 3, p. 193-201, jul./set. 2018.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 34. | | SOUZA, T. S. de; PINHEIRO, R. R.; LIMA, C. C. V. de; BRITO, R. L. L. de; AZEVEDO, D. A. A. de; DIAS, R. P.; SANTOS, V. W. S. dos; ANDRIOLI, A.; COSTA, J. N. Sheep infection by caprine lentivirus. Revista Brasileira de Saúde e Produção Animal, v. 19, n. 3, p. 268-276, jul./set. 2018.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 35. | | LIMA, C. C. V. de; AYRES, M. C. C; PINHEIRO, R. R.; COSTA, J. N.; ANDRIOLI, A.; SOUZA, T. S. de; AZEVEDO, D. A. A. de; SANTOS, V. W. S. dos; ARAÚJO, J. F.; SOUZA, A. L. M. de. Transmissão vertical do lentivirus caprino em ovinos. Biológico, São Paulo, v. 77, p. 120, 2015. Suplemento 2, ref. 239. Edição dos Resumos XI Congresso Brasileiro de Buiatria, XVII Congresso Latinoamericano de Buiatria, jul. 2015.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 36. | | SOUSA, A. L. M. de; PINHEIRO, R. R.; ARAÚJO, J. F.; AZEVEDO, D. A. A. de; PEIXOTO, R. M.; ANDRIOLI, A.; BEZERRA, S. T. da C. S.; TEIXEIRA, M. F. da S. Sodium dodecyl sulfate as a viral inactivator and future perspectives in the control of small ruminant lentiviruses. Arquivos do Instituto Biológico, v. 86, p. 1-9, e0752018, 2019.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 37. | | ARAÚJO, J. F.; ANDRIOLI, A.; PINHEIRO, R. R.; SIDER, L. H.; SOUSA, A. L. M. de; AZEVEDO, D. A. A. de; PEIXOTO, R. M.; LIMA, A. M. C.; DAMASCENO, E. M.; SOUZA, S. C. R.; TEIXEIRA, M. F. da S. Vertical transmissibility of small ruminant lentivirus. PLoSONE, v. 15, n. 11, e0239916, Nov. 2020.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 38. | | PINHEIRO, R. R.; AZEVEDO, D. A. A. de; SOUZA, A. L. de; ARAÚJO, J. F.; ANDRIOLI, A.; SIDER, L. H.; SOUZA, T. S. de; CRUZ, J. C. M. da. Uso de cultura celular de membrana nictitante caprina para produção de antígeno de lentivírus de pequenos ruminantes. Sobral: Embrapa Caprinos e Ovinos, 2012. (Embrapa Caprinos e Ovinos. Comunicado Técnico, 132).| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 39. | | SOUZA, T. S. de; PINHEIRO, R. R.; COSTA, J. N.; LIMA, C. C. V. de; ANDRIOLI, A.; AZEVEDO, D. A. A. de; ARAÚJO, J. F.; SOUSA, A. L. M. de; PINHEIRO, D. N. S.; FERNANDES, F. M. C.; COSTA NETO, A. O. Interspecific transmission of small ruminant lentiviruses from goats to sheep. Brazilian Journal of Microbiology, São Paulo, v. 46, n. 3, p. 867-874, July/Sept. 2015.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 40. | | SOUZA, T. S. de; COSTA, J. N.; PINHEIRO, R. R.; MELO, F. C. C. de; LIMA, C. C. V. de; ANDRIOLI, A.; AZEVEDO, D. A. A. de; SANTOS, V. W. S. dos; OLIVEIRA, E. L. de; COSTA NETO, A. de O. Duração da imunidade passiva para lentivírus de pequenos ruminantes em cordeiros. Semina: Ciências Agrárias, Londrina, v. 35, n. 2, p. 845-856, mar./abr. 2014.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 43 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
29/11/2020 |
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Data da última atualização: |
29/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
ARAÚJO, J. F.; ANDRIOLI, A.; PINHEIRO, R. R.; SIDER, L. H.; SOUSA, A. L. M. de; AZEVEDO, D. A. A. de; PEIXOTO, R. M.; LIMA, A. M. C.; DAMASCENO, E. M.; SOUZA, S. C. R.; TEIXEIRA, M. F. da S. |
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Afiliação: |
JUSCILÂNIA FURTADO ARAÚJO; ALICE ANDRIOLI, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; LUCIA HELENA SIDER, CNPC; ANA LÍDIA MADEIRA DE SOUSA; DALVA ALANA ARAGÃO DE AZEVEDO; RENATO MESQUITA PEIXOTO; ANA MILENA CESAR LIMA; EDGAR MARQUES DAMASCENO; SAMARA CRISTINA ROCHA SOUZA; MARIA FÁTIMA DA SILVA TEIXEIRA. |
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Título: |
Vertical transmissibility of small ruminant lentivirus. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
PLoSONE, v. 15, n. 11, e0239916, Nov. 2020. |
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DOI: |
https://doi.org/10.1371/journal.pone.0239916 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This study aimed to evaluate by means of Nested Polymerase Chain Reaction (nPCR), co-cultivation and sequencing, with genetic comparison between strains (mother/newborn), the occurrence of vertical transmission of Small Ruminant Lentiviruses (SRLV) from naturally occurring nannies infected for their offspring. For the detection of SRLV seropositive progenitors, blood was collected from 42 nannies in the final third of gestation in tubes with and without anticoagulant. The diagnostic tests used were Western Blot (WB) and nPCR. During the period of birth, the same blood collection procedure was performed on 73 newborns at zero hours of birth, with the same diagnostic tests. Seventeen blood samples from seven-day-old kids, proven positive for SRLV by nPCR, chosen at random, were subjected to coculture in goat synovial membrane (GSM) cells for 105 days. The pro-viral DNA extracted from the cell supernatant from the coculture was subjected to nPCR. For DNA sequencing from the nPCR products, nine positive samples were chosen at random, four nannies with their respective offspring, also positive. Each sample was performed in triplicate, thus generating 27 nPCR products of which only 19 were suitable for analysis. Among the 42 pregnant goats, in 50% (21/42) pro-viral DNA was detected by nPCR, while in the WB, only 7.14% (3/42) presented antibodies against SRLV. Regarding neonates, of the 73 kids, 34 (46.57%) were positive for the virus, using the nPCR technique, while in the serological test (WB), three positive animals (4.10%) were observed. The coculture of the 17 samples with a positive result in the nPCR was confirmed in viral isolation by amplification of the SRLV pro-viral DNA. When aligned, the pro-viral DNA sequences (nannies and their respective offspring) presented homology in relation to the standard strain CAEV Co. It was concluded that the transmission of SRLV through intrauterine route was potentially the source of infection in the newborn goats. MenosAbstract: This study aimed to evaluate by means of Nested Polymerase Chain Reaction (nPCR), co-cultivation and sequencing, with genetic comparison between strains (mother/newborn), the occurrence of vertical transmission of Small Ruminant Lentiviruses (SRLV) from naturally occurring nannies infected for their offspring. For the detection of SRLV seropositive progenitors, blood was collected from 42 nannies in the final third of gestation in tubes with and without anticoagulant. The diagnostic tests used were Western Blot (WB) and nPCR. During the period of birth, the same blood collection procedure was performed on 73 newborns at zero hours of birth, with the same diagnostic tests. Seventeen blood samples from seven-day-old kids, proven positive for SRLV by nPCR, chosen at random, were subjected to coculture in goat synovial membrane (GSM) cells for 105 days. The pro-viral DNA extracted from the cell supernatant from the coculture was subjected to nPCR. For DNA sequencing from the nPCR products, nine positive samples were chosen at random, four nannies with their respective offspring, also positive. Each sample was performed in triplicate, thus generating 27 nPCR products of which only 19 were suitable for analysis. Among the 42 pregnant goats, in 50% (21/42) pro-viral DNA was detected by nPCR, while in the WB, only 7.14% (3/42) presented antibodies against SRLV. Regarding neonates, of the 73 kids, 34 (46.57%) were positive for the virus, using the nPCR technique, while in t... Mostrar Tudo |
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Palavras-Chave: |
SRLV. |
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Thesaurus NAL: |
Genetic techniques and protocols; Goat diseases; Goats; Lentivirus; Sheep diseases. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/218347/1/CNPC-2020-Art-40.pdf
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Marc: |
LEADER 02915naa a2200325 a 4500
001 2127175
005 2020-11-29
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1371/journal.pone.0239916$2DOI
100 1 $aARAÚJO, J. F.
245 $aVertical transmissibility of small ruminant lentivirus.$h[electronic resource]
260 $c2020
520 $aAbstract: This study aimed to evaluate by means of Nested Polymerase Chain Reaction (nPCR), co-cultivation and sequencing, with genetic comparison between strains (mother/newborn), the occurrence of vertical transmission of Small Ruminant Lentiviruses (SRLV) from naturally occurring nannies infected for their offspring. For the detection of SRLV seropositive progenitors, blood was collected from 42 nannies in the final third of gestation in tubes with and without anticoagulant. The diagnostic tests used were Western Blot (WB) and nPCR. During the period of birth, the same blood collection procedure was performed on 73 newborns at zero hours of birth, with the same diagnostic tests. Seventeen blood samples from seven-day-old kids, proven positive for SRLV by nPCR, chosen at random, were subjected to coculture in goat synovial membrane (GSM) cells for 105 days. The pro-viral DNA extracted from the cell supernatant from the coculture was subjected to nPCR. For DNA sequencing from the nPCR products, nine positive samples were chosen at random, four nannies with their respective offspring, also positive. Each sample was performed in triplicate, thus generating 27 nPCR products of which only 19 were suitable for analysis. Among the 42 pregnant goats, in 50% (21/42) pro-viral DNA was detected by nPCR, while in the WB, only 7.14% (3/42) presented antibodies against SRLV. Regarding neonates, of the 73 kids, 34 (46.57%) were positive for the virus, using the nPCR technique, while in the serological test (WB), three positive animals (4.10%) were observed. The coculture of the 17 samples with a positive result in the nPCR was confirmed in viral isolation by amplification of the SRLV pro-viral DNA. When aligned, the pro-viral DNA sequences (nannies and their respective offspring) presented homology in relation to the standard strain CAEV Co. It was concluded that the transmission of SRLV through intrauterine route was potentially the source of infection in the newborn goats.
650 $aGenetic techniques and protocols
650 $aGoat diseases
650 $aGoats
650 $aLentivirus
650 $aSheep diseases
653 $aSRLV
700 1 $aANDRIOLI, A.
700 1 $aPINHEIRO, R. R.
700 1 $aSIDER, L. H.
700 1 $aSOUSA, A. L. M. de
700 1 $aAZEVEDO, D. A. A. de
700 1 $aPEIXOTO, R. M.
700 1 $aLIMA, A. M. C.
700 1 $aDAMASCENO, E. M.
700 1 $aSOUZA, S. C. R.
700 1 $aTEIXEIRA, M. F. da S.
773 $tPLoSONE$gv. 15, n. 11, e0239916, Nov. 2020.
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Embrapa Caprinos e Ovinos (CNPC) |
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