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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
06/11/2019 |
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Data da última atualização: |
06/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BACILA, D. M.; CUNHA JUNIOR, A.; GRESSLER, V.; SCHEUERMANN, G. N.; COLDEBELLA, A.; CARON, L.; MAFRA, L. I.; FEDDERN, V. |
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Afiliação: |
DANNIELE MIRANDA BACILA, UFPR; ANILDO CUNHA JUNIOR, CNPSA; VANESSA GRESSLER, CNPSA; GERSON NEUDI SCHEUERMANN, CNPSA; ARLEI COLDEBELLA, CNPSA; LUIZINHO CARON, CNPSA; LUCIANA IGARASHI MAFRA, UFPR; VIVIAN FEDDERN, CNPSA. |
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Título: |
Detection of p-nitroaniline released from degradation of 4,4 - dinitrocarbanilide in chicken breast during thermal processing. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Journal of Agricultural and Food Chemistry, v. 67, p. 9002-9008, 2019. |
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DOI: |
10.1021/acs.jafc.9b02259 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The diphenylurea 4,4?-dinitrocarbanilide (DNC) is the residue of concern left in edible tissues of broilers fed diets containing the anticoccidial nicarbazin. When chicken meat is submitted to thermal processing, p-nitroaniline (p-NA) is expected from DNC degradation. This work aimed at evaluating whether thermal processing of DNC-containing chicken meat induces p-NA appearance. First, a hydrolysis assay was performed in aqueous solutions at 100 °C in different pH, confirming that DNC cleavage yields p-NA. Then a novel LC-MS/MS method was used to detect traces of this aromatic amine in DNCcontaining chicken breast fillets subjected to cooking methods. Our evidence showed p-NA occurrence in such chicken meat samples, which corroborated results from hydrolysis assay. The p-NA appearance in fillets was rather discrete during boiling treatment, but its concentration became pronounced over time for grilling, frying, and roasting, achieving respectively 326.3, 640.0, and 456.9 ?g/kg. As far as we are concerned, no other research identified degradation products from DNC residue in heat-processed chicken fillets. Therefore, this study leads to additional approaches to assess impacts on food safety. |
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Palavras-Chave: |
Cozimento de alimento; DNC; Filé de frango; LC-MS / MS; Método de cozimento; Nicarbazina; P-NA. |
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Thesagro: |
Frango de Corte; Segurança Alimentar; Temperatura. |
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Thesaurus Nal: |
Broiler chickens; Chicken meat; Cooking; Food safety; Nicarbazin. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02361naa a2200397 a 4500
001 2113949
005 2019-11-06
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1021/acs.jafc.9b02259$2DOI
100 1 $aBACILA, D. M.
245 $aDetection of p-nitroaniline released from degradation of 4,4 - dinitrocarbanilide in chicken breast during thermal processing.$h[electronic resource]
260 $c2019
520 $aAbstract: The diphenylurea 4,4?-dinitrocarbanilide (DNC) is the residue of concern left in edible tissues of broilers fed diets containing the anticoccidial nicarbazin. When chicken meat is submitted to thermal processing, p-nitroaniline (p-NA) is expected from DNC degradation. This work aimed at evaluating whether thermal processing of DNC-containing chicken meat induces p-NA appearance. First, a hydrolysis assay was performed in aqueous solutions at 100 °C in different pH, confirming that DNC cleavage yields p-NA. Then a novel LC-MS/MS method was used to detect traces of this aromatic amine in DNCcontaining chicken breast fillets subjected to cooking methods. Our evidence showed p-NA occurrence in such chicken meat samples, which corroborated results from hydrolysis assay. The p-NA appearance in fillets was rather discrete during boiling treatment, but its concentration became pronounced over time for grilling, frying, and roasting, achieving respectively 326.3, 640.0, and 456.9 ?g/kg. As far as we are concerned, no other research identified degradation products from DNC residue in heat-processed chicken fillets. Therefore, this study leads to additional approaches to assess impacts on food safety.
650 $aBroiler chickens
650 $aChicken meat
650 $aCooking
650 $aFood safety
650 $aNicarbazin
650 $aFrango de Corte
650 $aSegurança Alimentar
650 $aTemperatura
653 $aCozimento de alimento
653 $aDNC
653 $aFilé de frango
653 $aLC-MS / MS
653 $aMétodo de cozimento
653 $aNicarbazina
653 $aP-NA
700 1 $aCUNHA JUNIOR, A.
700 1 $aGRESSLER, V.
700 1 $aSCHEUERMANN, G. N.
700 1 $aCOLDEBELLA, A.
700 1 $aCARON, L.
700 1 $aMAFRA, L. I.
700 1 $aFEDDERN, V.
773 $tJournal of Agricultural and Food Chemistry$gv. 67, p. 9002-9008, 2019.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Pecuária Sudeste. |
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Data corrente: |
06/04/2020 |
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Data da última atualização: |
20/04/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
OLIVEIRA, F. A. de; VIGNA, B. B. Z.; SILVA, C. C. da; FAVERO, A. P.; MATTA, F. de P.; AZEVEDO, A. L. S.; SOUZA, A. P. de. |
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Afiliação: |
Fernanda A. de Oliveira, UNICAMP; BIANCA BACCILI ZANOTTO VIGNA, CPPSE; Carla C. da Silva, UNICAMP; ALESSANDRA PEREIRA FAVERO, CPPSE; FREDERICO DE PINA MATTA, CPPSE; ANA LUISA SOUSA AZEVEDO, CNPGL; Anete P. de Souza, UNICAMP. |
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Título: |
Coexpression and transcriptome analyses identify active apomixis-related genes in Paspalum notatum leaves. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
BMC Genomics, v. 21, n. 78, 2020. |
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Páginas: |
15 p. |
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DOI: |
https://doi.org/10.1186/s12864-020-6518-z |
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Idioma: |
Inglês |
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Conteúdo: |
Background - Paspalum notatum exhibits both sexual and apomictic cytotypes and, thus, is considered a good model for studies of apomixis because it facilitates comparative approaches. In this work, transcriptome sequencing was used to compare contrasting P. notatum cytotypes to identify differential expression patterns and candidate genes involved in the regulation of expression of this trait. Results - We built a comprehensive transcriptome using leaf and inflorescence from apomictic tetraploids and sexual diploids/tetraploids and a coexpression network based on pairwise correlations between transcript expression profiles. We identified genes exclusively expressed in each cytotype and genes differentially expressed between pairs of cytotypes. Gene Ontology enrichment analyses were performed to better interpret the data. We de novo assembled 114,306 reference transcripts. In total, 536 candidate genes possibly associated with apomixis were detected through statistical analyses of the differential expression data, and several interacting genes potentially linked to the apomixis-controlling region, genes that have already been reported in the literature, and their neighbors were transcriptionally related in the coexpression network. Conclusions - Apomixis is a highly desirable trait in modern agriculture due to the maintenance of the characteristics of the mother plant in the progeny. The reference transcriptome, candidate genes and their coexpression network identified in this work represent rich resources for future grass breeding programs. MenosBackground - Paspalum notatum exhibits both sexual and apomictic cytotypes and, thus, is considered a good model for studies of apomixis because it facilitates comparative approaches. In this work, transcriptome sequencing was used to compare contrasting P. notatum cytotypes to identify differential expression patterns and candidate genes involved in the regulation of expression of this trait. Results - We built a comprehensive transcriptome using leaf and inflorescence from apomictic tetraploids and sexual diploids/tetraploids and a coexpression network based on pairwise correlations between transcript expression profiles. We identified genes exclusively expressed in each cytotype and genes differentially expressed between pairs of cytotypes. Gene Ontology enrichment analyses were performed to better interpret the data. We de novo assembled 114,306 reference transcripts. In total, 536 candidate genes possibly associated with apomixis were detected through statistical analyses of the differential expression data, and several interacting genes potentially linked to the apomixis-controlling region, genes that have already been reported in the literature, and their neighbors were transcriptionally related in the coexpression network. Conclusions - Apomixis is a highly desirable trait in modern agriculture due to the maintenance of the characteristics of the mother plant in the progeny. The reference transcriptome, candidate genes and their coexpression network identified in thi... Mostrar Tudo |
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Palavras-Chave: |
Differential expression; Gene coexpression network; RNA sequencing. |
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Thesaurus NAL: |
Apomixis; Paspalum. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/212171/1/CoexpressionTranscriptomeAnalyses.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1129868/1/Coexpression-and-transcriptome-analyses-identify-active-apomixis-related-genes-in-Paspalum.pdf
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Marc: |
LEADER 02397naa a2200277 a 4500
001 2121557
005 2020-04-20
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1186/s12864-020-6518-z$2DOI
100 1 $aOLIVEIRA, F. A. de
245 $aCoexpression and transcriptome analyses identify active apomixis-related genes in Paspalum notatum leaves.$h[electronic resource]
260 $c2020
300 $a15 p.
520 $aBackground - Paspalum notatum exhibits both sexual and apomictic cytotypes and, thus, is considered a good model for studies of apomixis because it facilitates comparative approaches. In this work, transcriptome sequencing was used to compare contrasting P. notatum cytotypes to identify differential expression patterns and candidate genes involved in the regulation of expression of this trait. Results - We built a comprehensive transcriptome using leaf and inflorescence from apomictic tetraploids and sexual diploids/tetraploids and a coexpression network based on pairwise correlations between transcript expression profiles. We identified genes exclusively expressed in each cytotype and genes differentially expressed between pairs of cytotypes. Gene Ontology enrichment analyses were performed to better interpret the data. We de novo assembled 114,306 reference transcripts. In total, 536 candidate genes possibly associated with apomixis were detected through statistical analyses of the differential expression data, and several interacting genes potentially linked to the apomixis-controlling region, genes that have already been reported in the literature, and their neighbors were transcriptionally related in the coexpression network. Conclusions - Apomixis is a highly desirable trait in modern agriculture due to the maintenance of the characteristics of the mother plant in the progeny. The reference transcriptome, candidate genes and their coexpression network identified in this work represent rich resources for future grass breeding programs.
650 $aApomixis
650 $aPaspalum
653 $aDifferential expression
653 $aGene coexpression network
653 $aRNA sequencing
700 1 $aVIGNA, B. B. Z.
700 1 $aSILVA, C. C. da
700 1 $aFAVERO, A. P.
700 1 $aMATTA, F. de P.
700 1 $aAZEVEDO, A. L. S.
700 1 $aSOUZA, A. P. de
773 $tBMC Genomics$gv. 21, n. 78, 2020.
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Embrapa Pecuária Sudeste (CPPSE) |
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