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Registro Completo |
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
15/02/2019 |
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Data da última atualização: |
15/02/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BANDEIRA, A. M. P.; MATOS, J. E.; MARIA, A. N.; CARNEIRO, P. C. F.; PURDY, P. H.; AZEVEDO, H. C. |
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Afiliação: |
ANDERSON MARQUES PINTO BANDEIRA, UFS; JOSE EDUARDO MATOS, UFS; ALEXANDRE NIZIO MARIA, CPATC; PAULO CESAR FALANGHE CARNEIRO, CPATC; PHILLIP H. PURDY, ARS; HYMERSON COSTA AZEVEDO, CPATC. |
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Título: |
The effects of gelatin supplementation prior to cooling on ram semen quality and fertility. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Animal Reproduction, v. 15, n. 1, p. 23-28, Jan./Mar. 2018. |
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DOI: |
10.21451/1984-3143-AR2017-0021 |
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Idioma: |
Inglês |
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Conteúdo: |
The physical and chemical characteristics of gelatin have been used to justify its inclusion in extenders to preserve the sperm quality and improve results of cervical artificial insemination with cooled semen. The objective of this study was to evaluate the effects of gelatin supplementation in cooling extender on the quality and fertility of ram semen stored at 5°C. Semen samples (n = 24) of Santa Inês rams (n = 6) were diluted in Glycine-Yolk-Milk extender without (control) or with 1.5% of gelatin. The samples were loaded into 0.25 mL straws, cooled to 5°C and stored vertically for 48 and 72 hours. Sample quality was evaluated using straw homogeneity tests based on pH, osmolality and the proportion of spermatozoa (PS) in both upper and lower segments of straws (US and LS), analyses of sperm motility, plasma and acrosomal membrane integrity, and by fertility after artificial insemination. Differences between the US and LS of straws were found for pH and PS (%). They were significant only in the control group at both times: pH ? 5.96 vs. 5.71 at 48 h and 6.13 vs. 5.89 at 72 h; PS ? 21.66 vs. 78.34 at 48 h and 20.87 vs. 79.13 at 72 h. Storage in gelatin had very little, to no effect on the sperm kinetics or on the sperm membrane integrity evaluations. The addition of gelatin to the extender did not affect the pregnancy rate which ranged from 4.4 to 26.1%. We conclude that gelatin is effective in maintaining the physical and chemical homogeneity of the semen samples. Further research is needed in order to optimize the use of gelatin supplementation and elucidate any potential benefits. MenosThe physical and chemical characteristics of gelatin have been used to justify its inclusion in extenders to preserve the sperm quality and improve results of cervical artificial insemination with cooled semen. The objective of this study was to evaluate the effects of gelatin supplementation in cooling extender on the quality and fertility of ram semen stored at 5°C. Semen samples (n = 24) of Santa Inês rams (n = 6) were diluted in Glycine-Yolk-Milk extender without (control) or with 1.5% of gelatin. The samples were loaded into 0.25 mL straws, cooled to 5°C and stored vertically for 48 and 72 hours. Sample quality was evaluated using straw homogeneity tests based on pH, osmolality and the proportion of spermatozoa (PS) in both upper and lower segments of straws (US and LS), analyses of sperm motility, plasma and acrosomal membrane integrity, and by fertility after artificial insemination. Differences between the US and LS of straws were found for pH and PS (%). They were significant only in the control group at both times: pH ? 5.96 vs. 5.71 at 48 h and 6.13 vs. 5.89 at 72 h; PS ? 21.66 vs. 78.34 at 48 h and 20.87 vs. 79.13 at 72 h. Storage in gelatin had very little, to no effect on the sperm kinetics or on the sperm membrane integrity evaluations. The addition of gelatin to the extender did not affect the pregnancy rate which ranged from 4.4 to 26.1%. We conclude that gelatin is effective in maintaining the physical and chemical homogeneity of the semen samples. Further ... Mostrar Tudo |
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Thesagro: |
Fertilidade Animal; Sêmen. |
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Thesaurus Nal: |
Animal fertility. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/192876/1/The-effects-of-gelatin-supplementation-p23-28-AR-2017-0021.pdf
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Marc: |
LEADER 02312naa a2200229 a 4500
001 2106085
005 2019-02-15
008 2018 bl uuuu u00u1 u #d
024 7 $a10.21451/1984-3143-AR2017-0021$2DOI
100 1 $aBANDEIRA, A. M. P.
245 $aThe effects of gelatin supplementation prior to cooling on ram semen quality and fertility.$h[electronic resource]
260 $c2018
520 $aThe physical and chemical characteristics of gelatin have been used to justify its inclusion in extenders to preserve the sperm quality and improve results of cervical artificial insemination with cooled semen. The objective of this study was to evaluate the effects of gelatin supplementation in cooling extender on the quality and fertility of ram semen stored at 5°C. Semen samples (n = 24) of Santa Inês rams (n = 6) were diluted in Glycine-Yolk-Milk extender without (control) or with 1.5% of gelatin. The samples were loaded into 0.25 mL straws, cooled to 5°C and stored vertically for 48 and 72 hours. Sample quality was evaluated using straw homogeneity tests based on pH, osmolality and the proportion of spermatozoa (PS) in both upper and lower segments of straws (US and LS), analyses of sperm motility, plasma and acrosomal membrane integrity, and by fertility after artificial insemination. Differences between the US and LS of straws were found for pH and PS (%). They were significant only in the control group at both times: pH ? 5.96 vs. 5.71 at 48 h and 6.13 vs. 5.89 at 72 h; PS ? 21.66 vs. 78.34 at 48 h and 20.87 vs. 79.13 at 72 h. Storage in gelatin had very little, to no effect on the sperm kinetics or on the sperm membrane integrity evaluations. The addition of gelatin to the extender did not affect the pregnancy rate which ranged from 4.4 to 26.1%. We conclude that gelatin is effective in maintaining the physical and chemical homogeneity of the semen samples. Further research is needed in order to optimize the use of gelatin supplementation and elucidate any potential benefits.
650 $aAnimal fertility
650 $aFertilidade Animal
650 $aSêmen
700 1 $aMATOS, J. E.
700 1 $aMARIA, A. N.
700 1 $aCARNEIRO, P. C. F.
700 1 $aPURDY, P. H.
700 1 $aAZEVEDO, H. C.
773 $tAnimal Reproduction$gv. 15, n. 1, p. 23-28, Jan./Mar. 2018.
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Registro original: |
Embrapa Tabuleiros Costeiros (CPATC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura; Embrapa Semiárido. |
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Data corrente: |
30/11/2021 |
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Data da última atualização: |
30/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
BASTOS, D. C.; SOMBRA, K. E. S.; LIMA, M. A. C. de; PASSOS, O. S.; CALGARO, M.; ATAIDE, E. M. |
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Afiliação: |
DEBORA COSTA BASTOS, CPATSA; KASSIO EWERTON SANTOS SOMBRA; MARIA AUXILIADORA COELHO DE LIMA, CPATSA; ORLANDO SAMPAIO PASSOS, CNPMF; MARCELO CALGARO, CPATSA; ELMA MACHADO ATAIDE. |
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Título: |
Physicochemical characterization of Pera orange fruits selections grafted on two rootstocks in the São Francisco Valley, Brazil. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Comunicata Scientiae, v. 12, e3573, 2021. |
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ISSN: |
2177-5133 |
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DOI: |
https://doi.org/10.14295/CS.v12.3573 |
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Idioma: |
Inglês |
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Conteúdo: |
?Pera? orange is a scion variety widely grown in commercial orchards of various citrus-producing regions in Brazil due to the good acceptance of its fruits by the consumer market, both for fresh consumption and industrial processing. The development of clones or selections occurs by genetic mutations within genetic improvement studies with the different varieties, consisting of the selection of plants with superior quality and production characteristics to the variety of origin. In this perspective, this study aimed to evaluate the physicochemical characteristics of four ?Pera? orange selections (?D9?, ?D12?, ?D25?, and ?C21?) grafted on two rootstocks (Rangpur lime (Citrus limonia Osbeck) and ?Volkamer? lemon (Citrus volkameriana Tan. and Pasq.), under the semi-arid conditions of the São Francisco Valley, Brazil. The experimental design was completely randomized, in a 4 x 2 factorial arrangement, with the first factor corresponding to the orange selections (?Pera D9?, ?Pera D12?, ?Pera D25?, and ?Pera C21?) and the second factor corresponding to the Rangpur lime and ?Volkamer? lemon rootstocks, using five replications of 10 fruits. The physical characteristics evaluated were: fruit mass (g), diameter, and length (mm); fruit firmness (Lb), peel thickness (mm), number of seeds, and juice yield (%). The chemical parameters were determined through the contents of vitamin C, soluble solids (oBrix), and titratable acidity (g of citric acid 100 mL-1), also calculating the maturation index (SS/TA ratio). The interaction between rootstock varieties and orange scions (selections) induced differences in the physical and chemical attributes of fruits, with the Rangpur lime rootstock inducing better fruits for the orange selection ?Pera D-9?, which showed the highest values of fruit mass and diameter, associated with thicker peels, lower soluble solids content, lower acidity, and higher vitamin C content, while inducing higher values of fruit length and soluble solids content in the fruits of the orange selection ?Pera C-21?, and lower number of seeds and higher juice yield for the orange selection ?Pera D-12?. Based on these results, under these conditions, the physical and chemical characteristics observed in the fruits of the ?Pera? orange selections meet the standards required by the market of fresh fruits. Menos?Pera? orange is a scion variety widely grown in commercial orchards of various citrus-producing regions in Brazil due to the good acceptance of its fruits by the consumer market, both for fresh consumption and industrial processing. The development of clones or selections occurs by genetic mutations within genetic improvement studies with the different varieties, consisting of the selection of plants with superior quality and production characteristics to the variety of origin. In this perspective, this study aimed to evaluate the physicochemical characteristics of four ?Pera? orange selections (?D9?, ?D12?, ?D25?, and ?C21?) grafted on two rootstocks (Rangpur lime (Citrus limonia Osbeck) and ?Volkamer? lemon (Citrus volkameriana Tan. and Pasq.), under the semi-arid conditions of the São Francisco Valley, Brazil. The experimental design was completely randomized, in a 4 x 2 factorial arrangement, with the first factor corresponding to the orange selections (?Pera D9?, ?Pera D12?, ?Pera D25?, and ?Pera C21?) and the second factor corresponding to the Rangpur lime and ?Volkamer? lemon rootstocks, using five replications of 10 fruits. The physical characteristics evaluated were: fruit mass (g), diameter, and length (mm); fruit firmness (Lb), peel thickness (mm), number of seeds, and juice yield (%). The chemical parameters were determined through the contents of vitamin C, soluble solids (oBrix), and titratable acidity (g of citric acid 100 mL-1), also calculating the maturati... Mostrar Tudo |
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Palavras-Chave: |
Citros; Consumo in natura; Semiárido. |
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Thesagro: |
Citricultura; Laranja; Laranja Doce; Laranja Pêra; Melhoramento Genético Vegetal; Porta Enxerto; Pós-Colheita. |
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Thesaurus NAL: |
Citrus; Oranges; Postharvest physiology; Postharvest technology. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/228260/1/Physicochemical-characterization-2021.pdf
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Marc: |
LEADER 03425naa a2200373 a 4500
001 2136800
005 2021-11-30
008 2021 bl uuuu u00u1 u #d
022 $a2177-5133
024 7 $ahttps://doi.org/10.14295/CS.v12.3573$2DOI
100 1 $aBASTOS, D. C.
245 $aPhysicochemical characterization of Pera orange fruits selections grafted on two rootstocks in the São Francisco Valley, Brazil.$h[electronic resource]
260 $c2021
520 $a?Pera? orange is a scion variety widely grown in commercial orchards of various citrus-producing regions in Brazil due to the good acceptance of its fruits by the consumer market, both for fresh consumption and industrial processing. The development of clones or selections occurs by genetic mutations within genetic improvement studies with the different varieties, consisting of the selection of plants with superior quality and production characteristics to the variety of origin. In this perspective, this study aimed to evaluate the physicochemical characteristics of four ?Pera? orange selections (?D9?, ?D12?, ?D25?, and ?C21?) grafted on two rootstocks (Rangpur lime (Citrus limonia Osbeck) and ?Volkamer? lemon (Citrus volkameriana Tan. and Pasq.), under the semi-arid conditions of the São Francisco Valley, Brazil. The experimental design was completely randomized, in a 4 x 2 factorial arrangement, with the first factor corresponding to the orange selections (?Pera D9?, ?Pera D12?, ?Pera D25?, and ?Pera C21?) and the second factor corresponding to the Rangpur lime and ?Volkamer? lemon rootstocks, using five replications of 10 fruits. The physical characteristics evaluated were: fruit mass (g), diameter, and length (mm); fruit firmness (Lb), peel thickness (mm), number of seeds, and juice yield (%). The chemical parameters were determined through the contents of vitamin C, soluble solids (oBrix), and titratable acidity (g of citric acid 100 mL-1), also calculating the maturation index (SS/TA ratio). The interaction between rootstock varieties and orange scions (selections) induced differences in the physical and chemical attributes of fruits, with the Rangpur lime rootstock inducing better fruits for the orange selection ?Pera D-9?, which showed the highest values of fruit mass and diameter, associated with thicker peels, lower soluble solids content, lower acidity, and higher vitamin C content, while inducing higher values of fruit length and soluble solids content in the fruits of the orange selection ?Pera C-21?, and lower number of seeds and higher juice yield for the orange selection ?Pera D-12?. Based on these results, under these conditions, the physical and chemical characteristics observed in the fruits of the ?Pera? orange selections meet the standards required by the market of fresh fruits.
650 $aCitrus
650 $aOranges
650 $aPostharvest physiology
650 $aPostharvest technology
650 $aCitricultura
650 $aLaranja
650 $aLaranja Doce
650 $aLaranja Pêra
650 $aMelhoramento Genético Vegetal
650 $aPorta Enxerto
650 $aPós-Colheita
653 $aCitros
653 $aConsumo in natura
653 $aSemiárido
700 1 $aSOMBRA, K. E. S.
700 1 $aLIMA, M. A. C. de
700 1 $aPASSOS, O. S.
700 1 $aCALGARO, M.
700 1 $aATAIDE, E. M.
773 $tComunicata Scientiae$gv. 12, e3573, 2021.
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