|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
24/04/2021 |
Data da última atualização: |
12/07/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
TÁVORA, F. T. P. K.; BEVITORI, R.; MELLO, R. N. de; CINTRA, M. M. D. F.; OLIVEIRA NETO, O. B.; FONTES, W.; CASTRO, M. S.; SOUSA, M. V.; FRANCO, O. L.; REIS, A. M. dos. |
Afiliação: |
FABIANO T. P. K. TÁVORA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA, MG; ROSANGELA BEVITORI, CNPAF; RAQUEL NEVES DE MELLO, CNPAF; MARIA MONICA DOMINGUES F CINTRA, CNPAF; OSMUNDO B. OLIVEIRA NETO, CENTRO UNIVERSITARIO UNIEURO, Brasília-DF; WAGNER FONTES, UNIVERSIDADE DE BRASÍLIA; MARIANA S. CASTRO, UNIVERSIDADE DE BRASÍLIA; MARCELO V. SOUSA, UNIVERSIDADE DE BRASÍLIA; OCTAVIO L. FRANCO, UNIVERSIDADE CATÓLICA DE BRASÍLIA; ANGELA MEHTA DOS REIS, Cenargen. |
Título: |
Shotgun proteomics coupled to transient-inducible gene silencing reveal rice susceptibility genes as new sources for blast disease resistance. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Journal of Proteomics, v. 241, 104223, June 2021. |
ISSN: |
1874-3919 |
DOI: |
https://doi.org/10.1016/j.jprot.2021.104223 |
Idioma: |
Inglês Português |
Notas: |
Na publicação: Angela Mehta. |
Conteúdo: |
A comparative proteomic analysis between two near-isogenic rice lines, displaying a resistant and susceptible phenotype upon infection with Magnaporthe oryzae was performed. We identified and validated factors associ-ated with rice disease susceptibility, representing a flourishing source toward a more resolute rice-blast resis-tance. Proteome profiles were remarkably different during early infection (12 h post-inoculation), revealing several proteins with increased abundance in the compatible interaction. Potential players of rice susceptibility were selected and gene expression was evaluated by RT-qPCR. Gene Ontology analysis disclosed susceptibility gene-encoded proteins claimed to be involved in fungus sustenance and suppression of plant immunity, such as sucrose synthase 4-like, serpin-ZXA-like, nudix hydrolase15, and DjA2 chaperone protein. Two other candidate genes, picked from a previous transcriptome study, were added into our downstream analysis including pyr-abactin resistant-like 5 (OsPYL5), and rice ethylene-responsive factor 104 (OsERF104). Further, we validated their role in susceptibility by Transient-Induced Gene Silencing (TIGS) using short antisense oligodeoxyr-ibonucleotides that resulted in a remarkable reduction of foliar disease symptoms in the compatible interaction. Therefore, we successfully employed shotgun proteomics and antisense-based gene silencing to prospect and functionally validate rice potential susceptibility factors, which could be further explored to build rice-blast resistance. Significance: R gene-mediated disease resistance is race-specific and often not durable in the field. More recently, advancements in new breeding techniques (NBTs) have made plant disease susceptibility genes (S-genes) a new target to build a broad spectrum and more durable resistance, hence an alternative source to R-genes in breeding programs. We successfully coupled shotgun proteomics and gene silencing tools to prospect and validate new rice-bast susceptibility genes that can be further exploited toward a more resolute blast disease resistance. MenosA comparative proteomic analysis between two near-isogenic rice lines, displaying a resistant and susceptible phenotype upon infection with Magnaporthe oryzae was performed. We identified and validated factors associ-ated with rice disease susceptibility, representing a flourishing source toward a more resolute rice-blast resis-tance. Proteome profiles were remarkably different during early infection (12 h post-inoculation), revealing several proteins with increased abundance in the compatible interaction. Potential players of rice susceptibility were selected and gene expression was evaluated by RT-qPCR. Gene Ontology analysis disclosed susceptibility gene-encoded proteins claimed to be involved in fungus sustenance and suppression of plant immunity, such as sucrose synthase 4-like, serpin-ZXA-like, nudix hydrolase15, and DjA2 chaperone protein. Two other candidate genes, picked from a previous transcriptome study, were added into our downstream analysis including pyr-abactin resistant-like 5 (OsPYL5), and rice ethylene-responsive factor 104 (OsERF104). Further, we validated their role in susceptibility by Transient-Induced Gene Silencing (TIGS) using short antisense oligodeoxyr-ibonucleotides that resulted in a remarkable reduction of foliar disease symptoms in the compatible interaction. Therefore, we successfully employed shotgun proteomics and antisense-based gene silencing to prospect and functionally validate rice potential susceptibility factors, which could be furth... Mostrar Tudo |
Palavras-Chave: |
Gene ontology; Plant immunity; PTO-based TIGS; RT-qPCR; S-gene; Transient-Induced gene silencing. |
Thesagro: |
Arroz; Brusone; Doença de Planta; Oryza Sativa. |
Thesaurus Nal: |
blast disease; disease resistance; foliar diseases; Magnaporthe oryzae; plant diseases and disorders; proteomics; rice. |
Categoria do assunto: |
-- S Ciências Biológicas |
Marc: |
LEADER 03484naa a2200469 a 4500 001 2131497 005 2021-07-12 008 2021 bl uuuu u00u1 u #d 022 $a1874-3919 024 7 $ahttps://doi.org/10.1016/j.jprot.2021.104223$2DOI 100 1 $aTÁVORA, F. T. P. K. 245 $aShotgun proteomics coupled to transient-inducible gene silencing reveal rice susceptibility genes as new sources for blast disease resistance.$h[electronic resource] 260 $c2021 500 $aNa publicação: Angela Mehta. 520 $aA comparative proteomic analysis between two near-isogenic rice lines, displaying a resistant and susceptible phenotype upon infection with Magnaporthe oryzae was performed. We identified and validated factors associ-ated with rice disease susceptibility, representing a flourishing source toward a more resolute rice-blast resis-tance. Proteome profiles were remarkably different during early infection (12 h post-inoculation), revealing several proteins with increased abundance in the compatible interaction. Potential players of rice susceptibility were selected and gene expression was evaluated by RT-qPCR. Gene Ontology analysis disclosed susceptibility gene-encoded proteins claimed to be involved in fungus sustenance and suppression of plant immunity, such as sucrose synthase 4-like, serpin-ZXA-like, nudix hydrolase15, and DjA2 chaperone protein. Two other candidate genes, picked from a previous transcriptome study, were added into our downstream analysis including pyr-abactin resistant-like 5 (OsPYL5), and rice ethylene-responsive factor 104 (OsERF104). Further, we validated their role in susceptibility by Transient-Induced Gene Silencing (TIGS) using short antisense oligodeoxyr-ibonucleotides that resulted in a remarkable reduction of foliar disease symptoms in the compatible interaction. Therefore, we successfully employed shotgun proteomics and antisense-based gene silencing to prospect and functionally validate rice potential susceptibility factors, which could be further explored to build rice-blast resistance. Significance: R gene-mediated disease resistance is race-specific and often not durable in the field. More recently, advancements in new breeding techniques (NBTs) have made plant disease susceptibility genes (S-genes) a new target to build a broad spectrum and more durable resistance, hence an alternative source to R-genes in breeding programs. We successfully coupled shotgun proteomics and gene silencing tools to prospect and validate new rice-bast susceptibility genes that can be further exploited toward a more resolute blast disease resistance. 650 $ablast disease 650 $adisease resistance 650 $afoliar diseases 650 $aMagnaporthe oryzae 650 $aplant diseases and disorders 650 $aproteomics 650 $arice 650 $aArroz 650 $aBrusone 650 $aDoença de Planta 650 $aOryza Sativa 653 $aGene ontology 653 $aPlant immunity 653 $aPTO-based TIGS 653 $aRT-qPCR 653 $aS-gene 653 $aTransient-Induced gene silencing 700 1 $aBEVITORI, R. 700 1 $aMELLO, R. N. de 700 1 $aCINTRA, M. M. D. F. 700 1 $aOLIVEIRA NETO, O. B. 700 1 $aFONTES, W. 700 1 $aCASTRO, M. S. 700 1 $aSOUSA, M. V. 700 1 $aFRANCO, O. L. 700 1 $aREIS, A. M. dos 773 $tJournal of Proteomics$gv. 241, 104223, June 2021.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registro Completo
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
28/11/2022 |
Data da última atualização: |
28/11/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
NASCIMENTO, R. Q.; RIBEIRO, C. V. D. M.; COLAUTO, N. B.; SILVA, L. da; LEMOS, P. V. F.; FERREIRA, E. de S.; LINDE, G. A.; MACHADO, B. A. S.; TAVARES, P. P. L. G.; BIASOTO, A. C. T.; GUEZ, M. A. U.; CARVALHO, N.; ASSIS, D. de J.; SILVA, J. B. A. da; SOUZA, C. O. de. |
Afiliação: |
RENATA QUARTIERI NASCIMENTO, UNIVERSIDADE FEDERAL DA BAHIA; CLÁUDIO VAZ DI MAMBRO RIBEIRO, UNIVERSIDADE FEDERAL DA BAHIA; NELSON BARROS COLAUTO, UNIVERSIDADE FEDERAL DA BAHIA; LARISSA DA SILVA, UNIVERSIDADE FEDERAL DA BAHIA; PAULO VITOR FRANÇA LEMOS, UNIVERSIDADE FEDERAL DA BAHIA; EDERLAN DE SOUZA FERREIRA, UNIVERSIDADE FEDERAL DA BAHIA; GIANI ANDREA LINDE, UNIVERSIDADE FEDERAL DA BAHIA; BRUNA APARECIDA SOUZA MACHADO, SENAI; PEDRO PAULO LORDELO GUIMARÃES TAVARES, UNIVERSIDADE FEDERAL DA BAHIA; ALINE TELLES BIASOTO MARQUES, CNPMA; MARCELO ANDRES UMSZA GUEZ, UNIVERSIDADE FEDERAL DA BAHIA; NATÁLIA CARVALHO, UNIVERSIDADE FEDERAL DA BAHIA; DENÍLSON DE JESUS ASSIS, UNIVERSIDADE FEDERAL DA BAHIA; JANIA BETÂNIA ALVES DA SILVA, UNIVERSIDADE FEDERAL DO RECÔNCAVO DA BAHIA; CAROLINA OLIVEIRA DE SOUZA, UNIVERSIDADE FEDERAL DA BAHIA. |
Título: |
Utilization of agro-industrial residues in the rearing and nutritional enrichment of Zophobas atratus larvae: new food raw materials. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Molecules, v. 27, n. 20, article 6963,2022. |
ISSN: |
1420-3049 |
DOI: |
https://doi.org/10.3390/molecules27206963 |
Idioma: |
Inglês |
Conteúdo: |
Abstrcat: Edible insects are a potential alternative food source of high feed conversion efficiency and protein content. Zophobas atratus is an edible insect that adapts to different diets, enabling sustainable rearing by adding value to by-products and agro-industrial residues. This study aimed to evaluate the performance and nutritional characterization of Zophobas atratus larvae fed with different proportions of grape residue. Physicochemical analysis of the diets and larvae (AOAC procedures), fatty acid profile (chromatographic techniques), metals and non-metals (inductively coupled plasma optical emission spectrometry), larval mass gain, feed conversion efficiency, and mortality rate were assessed. The replacement of 25% of the conventional diet with grape residue increased lipid, ash, and fiber contents and reduced protein, carbohydrates, and energy. It promoted greater mass gain, lower mortality rate, and reduced larval growth time by 51%. Among the replacements, 25% resulted in the second-highest content of calcium, sodium, magnesium, and zinc, and the lowest content of potassium and phosphorus in the larvae. The 100% replacement resulted in the highest amounts of C18:2n6 (27.8%), C18:3n3 (2.2%), and PUFA (30.0%). Replacing 25% of the conventional diet with grape residue is equivalent to the conventional diet in many aspects and improves several larvae performance indices and nutritional values. |
Thesagro: |
Alimentação; Inseto; Nutrição Animal; Suplemento Alimentar; Uva. |
Thesaurus NAL: |
Crop residues; Economic sustainability; Edible insects; Food conversion; Grapes; Zophobas atratus. |
Categoria do assunto: |
O Insetos e Entomologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148850/1/Marques-Utilization-agro-industrial-2022.pdf
|
Marc: |
LEADER 02732naa a2200445 a 4500 001 2148850 005 2022-11-28 008 2022 bl uuuu u00u1 u #d 022 $a1420-3049 024 7 $ahttps://doi.org/10.3390/molecules27206963$2DOI 100 1 $aNASCIMENTO, R. Q. 245 $aUtilization of agro-industrial residues in the rearing and nutritional enrichment of Zophobas atratus larvae$bnew food raw materials.$h[electronic resource] 260 $c2022 520 $aAbstrcat: Edible insects are a potential alternative food source of high feed conversion efficiency and protein content. Zophobas atratus is an edible insect that adapts to different diets, enabling sustainable rearing by adding value to by-products and agro-industrial residues. This study aimed to evaluate the performance and nutritional characterization of Zophobas atratus larvae fed with different proportions of grape residue. Physicochemical analysis of the diets and larvae (AOAC procedures), fatty acid profile (chromatographic techniques), metals and non-metals (inductively coupled plasma optical emission spectrometry), larval mass gain, feed conversion efficiency, and mortality rate were assessed. The replacement of 25% of the conventional diet with grape residue increased lipid, ash, and fiber contents and reduced protein, carbohydrates, and energy. It promoted greater mass gain, lower mortality rate, and reduced larval growth time by 51%. Among the replacements, 25% resulted in the second-highest content of calcium, sodium, magnesium, and zinc, and the lowest content of potassium and phosphorus in the larvae. The 100% replacement resulted in the highest amounts of C18:2n6 (27.8%), C18:3n3 (2.2%), and PUFA (30.0%). Replacing 25% of the conventional diet with grape residue is equivalent to the conventional diet in many aspects and improves several larvae performance indices and nutritional values. 650 $aCrop residues 650 $aEconomic sustainability 650 $aEdible insects 650 $aFood conversion 650 $aGrapes 650 $aZophobas atratus 650 $aAlimentação 650 $aInseto 650 $aNutrição Animal 650 $aSuplemento Alimentar 650 $aUva 700 1 $aRIBEIRO, C. V. D. M. 700 1 $aCOLAUTO, N. B. 700 1 $aSILVA, L. da 700 1 $aLEMOS, P. V. F. 700 1 $aFERREIRA, E. de S. 700 1 $aLINDE, G. A. 700 1 $aMACHADO, B. A. S. 700 1 $aTAVARES, P. P. L. G. 700 1 $aBIASOTO, A. C. T. 700 1 $aGUEZ, M. A. U. 700 1 $aCARVALHO, N. 700 1 $aASSIS, D. de J. 700 1 $aSILVA, J. B. A. da 700 1 $aSOUZA, C. O. de 773 $tMolecules$gv. 27, n. 20, article 6963,2022.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Meio Ambiente (CNPMA) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|