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 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
16/08/2016 |
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Data da última atualização: |
11/07/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CADEMARTORI, P. H. G. de; CARVALHO, A. R. de; MARANGONI, P. R. D.; BERTON, M. A. C.; BLANCHET, P.; BOLZON DE MUNIZ, G. I.; MAGALHAES, W. L. E. |
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Afiliação: |
PEDRO HENRIQUE GONZALEZ DE CADEMARTORI, Wood and Forestry Science Center (PPGEF), UFPR; AGNE ROANI DE CARVALHO, SENAI Institute of Innovation in Electrochemistry; PAULO ROBERTO DANTAS MARANGONI, SENAI Institute of Innovation in Electrochemistry; MARCOS ANTONIO COELHO BERTON, SENAI Institute of Innovation in Electrochemistry; PIERRE BLANCHET, Université Laval; GRACIELA INÊS BOLZON DE MUNIZ, UFPR; WASHINGTON LUIZ ESTEVES MAGALHAES, CNPF. |
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Título: |
Adhesion performance and film formation of acrylic emulsion coating on mediumdensity fiberboard treated with Ar plasma. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
International Journal of Adhesion and Adhesives, v. 70, p. 322-328, Oct. 2016. |
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DOI: |
10.1016/j.ijadhadh.2016.08.002 |
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Idioma: |
Inglês |
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Conteúdo: |
This work investigates the adhesion properties of medium density fiberboard (MDF) panel surfaces treated by an argon plasma at two power levels (50 and 150 W) and four glow discharge times (10, 40, 60 and 120 s) and then coated with an acrylic emulsion. Wettability parameters and film formation kinetics were investigated by water apparent contact angle and diffusing wave spectroscopy, respectively. Pull-off strength was used to determine coating/wood adhesion. The aging effect of 300-days in storage at ambient conditions was examined by apparent contact angle measurements. The wettability of the MDF surface was enhanced significantly - especially at the 150 W power level ? that improves coating/wood adhesion. A film formation process occurred in shorter time for plasma treated samples due to the faster motion of particles. MDF panels partially recovered their natural hydrophobicity in the first ten days of storage. Nevertheless, higher levels of power and time of glow discharge were able to slow down the aging effects. |
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Palavras-Chave: |
Diffusing wave spectroscopy; Espectroscopia; MDF; Molhabilidade; Surface treatment; Tratamento da superfície. |
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Thesagro: |
Madeira. |
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Thesaurus Nal: |
Wettability. |
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Categoria do assunto: |
W Química e Física |
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Marc: |
LEADER 02002naa a2200301 a 4500
001 2051000
005 2017-07-11
008 2016 bl uuuu u00u1 u #d
024 7 $a10.1016/j.ijadhadh.2016.08.002$2DOI
100 1 $aCADEMARTORI, P. H. G. de
245 $aAdhesion performance and film formation of acrylic emulsion coating on mediumdensity fiberboard treated with Ar plasma.$h[electronic resource]
260 $c2016
520 $aThis work investigates the adhesion properties of medium density fiberboard (MDF) panel surfaces treated by an argon plasma at two power levels (50 and 150 W) and four glow discharge times (10, 40, 60 and 120 s) and then coated with an acrylic emulsion. Wettability parameters and film formation kinetics were investigated by water apparent contact angle and diffusing wave spectroscopy, respectively. Pull-off strength was used to determine coating/wood adhesion. The aging effect of 300-days in storage at ambient conditions was examined by apparent contact angle measurements. The wettability of the MDF surface was enhanced significantly - especially at the 150 W power level ? that improves coating/wood adhesion. A film formation process occurred in shorter time for plasma treated samples due to the faster motion of particles. MDF panels partially recovered their natural hydrophobicity in the first ten days of storage. Nevertheless, higher levels of power and time of glow discharge were able to slow down the aging effects.
650 $aWettability
650 $aMadeira
653 $aDiffusing wave spectroscopy
653 $aEspectroscopia
653 $aMDF
653 $aMolhabilidade
653 $aSurface treatment
653 $aTratamento da superfície
700 1 $aCARVALHO, A. R. de
700 1 $aMARANGONI, P. R. D.
700 1 $aBERTON, M. A. C.
700 1 $aBLANCHET, P.
700 1 $aBOLZON DE MUNIZ, G. I.
700 1 $aMAGALHAES, W. L. E.
773 $tInternational Journal of Adhesion and Adhesives$gv. 70, p. 322-328, Oct. 2016.
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Registro original: |
Embrapa Florestas (CNPF) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
19/02/2021 |
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Data da última atualização: |
07/02/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
ARRAES, F. B. M.; MARTINS-DE-SA, D; VASQUEZ, D. D. N.; MELO, B. P.; FAHEEM, M.; MACEDO, L. L. P. de; MORGANTE, C. V.; BARBOSA, J. A. R. G.; TOGAWA, R. C.; MOREIRA, V. J. V.; DANCHIN, E. G. J.; SA, M. F. G. de. |
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Afiliação: |
FABRICIO BARBOSA MONTEIRO ARRAES; DIOGO MARTINS-DE-SA; DANIEL D. NORIEGA VASQUEZ; BRUNO PAES MELO; MUHAMMAD FAHEEM; LEONARDO LIMA PEPINO DE MACEDO; CAROLINA VIANNA MORGANTE, CPATSA; JOÃO ALEXANDRE R. G. BARBOSA; ROBERTO COITI TOGAWA; VALDEIR JUNIO VAZ MOREIRA; ETIENNE G. J. DANCHIN; MARIA FATIMA GROSSI DE SA. |
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Título: |
Dissecting protein domain variability in the core rna interference machinery of five insect orders. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
RNA Biology, v. 18, n. 11, p. 1653-1681, 2021. |
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DOI: |
https://doi.org/10.1080/15476286.2020.1861816 |
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Idioma: |
Inglês |
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Conteúdo: |
RNA interference (RNAi)-mediated gene silencing can be used to control specific insect pest populations. Unfortunately, the variable efficiency in the knockdown levels of target genes has narrowed the applicability of this technology to a few species. Here, we examine the current state of knowledge regarding the miRNA (micro RNA) and siRNA (small interfering RNA) pathways in insects and investigate the structural variability at key protein domains of the RNAi machinery. Our goal was to correlate domain variability with mechanisms affecting the gene silencing efficiency. To this end, the protein domains of 168 insect species, encompassing the orders Coleoptera, Diptera, Hemiptera, Hymenoptera, and Lepidoptera, were analysed using our pipeline, which takes advantage of meticulous structure-based sequence alignments. We used phylogenetic inference and the evolutionary rate coefficient (K) to outline the variability across domain regions and surfaces. Our results show that four domains, namely dsrm, Helicase, PAZ and Ribonuclease III, are the main contributors of protein variability in the RNAi machinery across different insect orders. We discuss the potential roles of these domains in regulating RNAi-mediated gene silencing and the role of loop regions in fine-tuning RNAi efficiency. Additionally, we identified several order-specific singularities which indicate that lepidopterans have evolved differently from other insect orders, possibly due to constant coevolution with plants and viruses. In conclusion, our results highlight several variability hotspots that deserve further investigation in order to improve the application of RNAi technology in the control of insect pests. MenosRNA interference (RNAi)-mediated gene silencing can be used to control specific insect pest populations. Unfortunately, the variable efficiency in the knockdown levels of target genes has narrowed the applicability of this technology to a few species. Here, we examine the current state of knowledge regarding the miRNA (micro RNA) and siRNA (small interfering RNA) pathways in insects and investigate the structural variability at key protein domains of the RNAi machinery. Our goal was to correlate domain variability with mechanisms affecting the gene silencing efficiency. To this end, the protein domains of 168 insect species, encompassing the orders Coleoptera, Diptera, Hemiptera, Hymenoptera, and Lepidoptera, were analysed using our pipeline, which takes advantage of meticulous structure-based sequence alignments. We used phylogenetic inference and the evolutionary rate coefficient (K) to outline the variability across domain regions and surfaces. Our results show that four domains, namely dsrm, Helicase, PAZ and Ribonuclease III, are the main contributors of protein variability in the RNAi machinery across different insect orders. We discuss the potential roles of these domains in regulating RNAi-mediated gene silencing and the role of loop regions in fine-tuning RNAi efficiency. Additionally, we identified several order-specific singularities which indicate that lepidopterans have evolved differently from other insect orders, possibly due to constant coevolution with plant... Mostrar Tudo |
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Palavras-Chave: |
Argonaute; DCR1; Dicer; Drosha; DsRBDs; Evolução da proteína; In silico analysis; Loquacious; Pasha; Protein evolution; R2D2; RIIID II; Sequência de proteínas inteira; Structure-function relationship. |
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Thesagro: |
Controle Genético; Genoma; Inseto; Praga; Proteína. |
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Thesaurus NAL: |
Insect control; Pest control; Protein structure; Proteins. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/230917/1/Dissecting-protein-domain-variability-2021.pdf
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Marc: |
LEADER 03184naa a2200541 a 4500
001 2130159
005 2022-02-07
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1080/15476286.2020.1861816$2DOI
100 1 $aARRAES, F. B. M.
245 $aDissecting protein domain variability in the core rna interference machinery of five insect orders.$h[electronic resource]
260 $c2021
520 $aRNA interference (RNAi)-mediated gene silencing can be used to control specific insect pest populations. Unfortunately, the variable efficiency in the knockdown levels of target genes has narrowed the applicability of this technology to a few species. Here, we examine the current state of knowledge regarding the miRNA (micro RNA) and siRNA (small interfering RNA) pathways in insects and investigate the structural variability at key protein domains of the RNAi machinery. Our goal was to correlate domain variability with mechanisms affecting the gene silencing efficiency. To this end, the protein domains of 168 insect species, encompassing the orders Coleoptera, Diptera, Hemiptera, Hymenoptera, and Lepidoptera, were analysed using our pipeline, which takes advantage of meticulous structure-based sequence alignments. We used phylogenetic inference and the evolutionary rate coefficient (K) to outline the variability across domain regions and surfaces. Our results show that four domains, namely dsrm, Helicase, PAZ and Ribonuclease III, are the main contributors of protein variability in the RNAi machinery across different insect orders. We discuss the potential roles of these domains in regulating RNAi-mediated gene silencing and the role of loop regions in fine-tuning RNAi efficiency. Additionally, we identified several order-specific singularities which indicate that lepidopterans have evolved differently from other insect orders, possibly due to constant coevolution with plants and viruses. In conclusion, our results highlight several variability hotspots that deserve further investigation in order to improve the application of RNAi technology in the control of insect pests.
650 $aInsect control
650 $aPest control
650 $aProtein structure
650 $aProteins
650 $aControle Genético
650 $aGenoma
650 $aInseto
650 $aPraga
650 $aProteína
653 $aArgonaute
653 $aDCR1
653 $aDicer
653 $aDrosha
653 $aDsRBDs
653 $aEvolução da proteína
653 $aIn silico analysis
653 $aLoquacious
653 $aPasha
653 $aProtein evolution
653 $aR2D2
653 $aRIIID II
653 $aSequência de proteínas inteira
653 $aStructure-function relationship
700 1 $aMARTINS-DE-SA, D
700 1 $aVASQUEZ, D. D. N.
700 1 $aMELO, B. P.
700 1 $aFAHEEM, M.
700 1 $aMACEDO, L. L. P. de
700 1 $aMORGANTE, C. V.
700 1 $aBARBOSA, J. A. R. G.
700 1 $aTOGAWA, R. C.
700 1 $aMOREIRA, V. J. V.
700 1 $aDANCHIN, E. G. J.
700 1 $aSA, M. F. G. de
773 $tRNA Biology$gv. 18, n. 11, p. 1653-1681, 2021.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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