| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
|
Data corrente: |
03/07/2021 |
|
Data da última atualização: |
31/08/2021 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
VIANA, J. H. M.; PEREIRA, N. E. S.; FARIA, O. A. C.; DIAS, L. R. O.; OLIVEIRA, E. R.; FERNANDES, C. A. C.; SIQUEIRA, L. G. B. |
|
Afiliação: |
JOAO HENRIQUE MOREIRA VIANA, Cenargen; NATHALIA ELLEN SOUSA PEREIRA, UNB; OTAVIO AUGUSTO COSTA FARIA, UNB; LUZIA RENATA OLIVEIRA DIAS, UNB; EDUARDO RAMOS OLIVEIRA, UNIFENAS; CARLOS ANTONIO CARVALHO FERNANDES, UNIFENAS; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL. |
|
Título: |
Active immunization against GnRH as an alternative therapeutic approach for the management of Bos indicus oocyte donors diagnosed with chronic cystic ovarian disease. |
|
Ano de publicação: |
2021 |
|
Fonte/Imprenta: |
Theriogenology, v. 172, p. 133-141, 2021. |
|
DOI: |
https://doi.org/10.1016/j.theriogenology.2021.06.014 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The aim of this study was to evaluate the effect of active immunization against GnRH in Nelore (Bos indicus) cows repeatedly used as oocyte donors that developed chronic cystic ovarian disease (COD). In the first experiment, ovarian and uterine features were first compared between COD cows (n ¼ 15) and healthy cows (n ¼ 22, cycling control group) from the same breed and herd. Cows with COD had a greater number of large (P < 0.0001) and medium follicles (P < 0.01) but lesser small follicles (P < 0.05) than cycling controls. Mucometra was diagnosed in 73.3% of COD cows, but in none of the controls. No difference in average thickness of the endometrium was detected between groups; however, endometrial thickness and mucometra score were negatively correlated (R ¼ 0.73, P ¼ 0.0029) in COD cows. In the second experiment, COD cows were randomly allocated into two experimental groups, which received two 1.0 mL SC injections of either an anti-GnRH vaccine (COD immunized group, n ¼ 8) or saline (COD control group, n ¼ 7), given 28 days apart. Cows were examined weekly by transrectal ultrasonography during nine consecutive weeks after the first injection to evaluate the number and distribution of follicles among size classes, endometrial thickness, and presence of clinical mucometra. Vaccination against GnRH resulted in a progressive suppression of follicle growth and a reduction in the average size of the largest follicle, as well as in the number of large follicles (P < 0.01) in COD immunized cows compared with COD controls from week 7 onwards. Conversely, the number of small follicles in the COD immunized group increased after week 5 and was greater (P ¼ 0.0023) than controls on week 9. Endometrial thickness and mucometra score were not affected (P > 0.05) by immunization against GnRH. In the third experiment, the COD immunized cows with effective suppression of follicle growth four weeks after the 2nd injection (n ¼ 6) were submitted to three consecutive ovum pick-up (OPU) sessions (weeks 10, 11, and 12) for in vitro embryo production (IVEP). Cumulus-oocyte complexes (COC) collected from slaughterhouse ovaries were used as controls for IVEP. COD cows with produced 25.0 ± 3.8 COC per OPU session with no apparent detrimental effect of anti-GnRH vaccine on oocyte developmental potential in vitro, i.e., we observed similar cleavage rate (P ¼ 0.5914) and greater blastocyst rate (P ¼ 0.0177) in immunized cows compared with COC from slaughterhouse controls. Finally, in the fourth experiment wave emergence and follicular dynamics after follicle ablation were compared between COD immunized cows with effective suppression of follicle growth and a subset (n ¼ 6) of the cycling, control group. No follicles grew beyond 4 mm diameter after follicle ablation in the COD immunized group, whereas a normal follicular wave emergence occurred in cycling controls. Antral follicle count was similar between cycling controls and COD immunized groups at 24 h and 96 h post-follicle ablation (P > 0.05), but greater in cycling controls at 48 h and 72 h post-follicle ablation (P < 0.05). In summary, our results suggest that active immunization against GnRH is effective to induce the regression of follicular cysts as well as increase the number of small follicles growing on the ovaries, in oocyte donors diagnosed with chronic COD, with no apparent negative effect on oocyte developmental potential in vitro. MenosThe aim of this study was to evaluate the effect of active immunization against GnRH in Nelore (Bos indicus) cows repeatedly used as oocyte donors that developed chronic cystic ovarian disease (COD). In the first experiment, ovarian and uterine features were first compared between COD cows (n ¼ 15) and healthy cows (n ¼ 22, cycling control group) from the same breed and herd. Cows with COD had a greater number of large (P < 0.0001) and medium follicles (P < 0.01) but lesser small follicles (P < 0.05) than cycling controls. Mucometra was diagnosed in 73.3% of COD cows, but in none of the controls. No difference in average thickness of the endometrium was detected between groups; however, endometrial thickness and mucometra score were negatively correlated (R ¼ 0.73, P ¼ 0.0029) in COD cows. In the second experiment, COD cows were randomly allocated into two experimental groups, which received two 1.0 mL SC injections of either an anti-GnRH vaccine (COD immunized group, n ¼ 8) or saline (COD control group, n ¼ 7), given 28 days apart. Cows were examined weekly by transrectal ultrasonography during nine consecutive weeks after the first injection to evaluate the number and distribution of follicles among size classes, endometrial thickness, and presence of clinical mucometra. Vaccination against GnRH resulted in a progressive suppression of follicle growth and a reduction in the average size of the largest follicle, as well as in the number of large follicles (P < 0.01) in COD ... Mostrar Tudo |
|
Palavras-Chave: |
Follicle growth suppression; GnRH vaccine; In vitro embryo production; Nelore breed. |
|
Thesagro: |
Bovino; Doença Animal; Gado Nelore. |
|
Categoria do assunto: |
--
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 04414naa a2200289 a 4500
001 2133972
005 2021-08-31
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2021.06.014$2DOI
100 1 $aVIANA, J. H. M.
245 $aActive immunization against GnRH as an alternative therapeutic approach for the management of Bos indicus oocyte donors diagnosed with chronic cystic ovarian disease.$h[electronic resource]
260 $c2021
520 $aThe aim of this study was to evaluate the effect of active immunization against GnRH in Nelore (Bos indicus) cows repeatedly used as oocyte donors that developed chronic cystic ovarian disease (COD). In the first experiment, ovarian and uterine features were first compared between COD cows (n ¼ 15) and healthy cows (n ¼ 22, cycling control group) from the same breed and herd. Cows with COD had a greater number of large (P < 0.0001) and medium follicles (P < 0.01) but lesser small follicles (P < 0.05) than cycling controls. Mucometra was diagnosed in 73.3% of COD cows, but in none of the controls. No difference in average thickness of the endometrium was detected between groups; however, endometrial thickness and mucometra score were negatively correlated (R ¼ 0.73, P ¼ 0.0029) in COD cows. In the second experiment, COD cows were randomly allocated into two experimental groups, which received two 1.0 mL SC injections of either an anti-GnRH vaccine (COD immunized group, n ¼ 8) or saline (COD control group, n ¼ 7), given 28 days apart. Cows were examined weekly by transrectal ultrasonography during nine consecutive weeks after the first injection to evaluate the number and distribution of follicles among size classes, endometrial thickness, and presence of clinical mucometra. Vaccination against GnRH resulted in a progressive suppression of follicle growth and a reduction in the average size of the largest follicle, as well as in the number of large follicles (P < 0.01) in COD immunized cows compared with COD controls from week 7 onwards. Conversely, the number of small follicles in the COD immunized group increased after week 5 and was greater (P ¼ 0.0023) than controls on week 9. Endometrial thickness and mucometra score were not affected (P > 0.05) by immunization against GnRH. In the third experiment, the COD immunized cows with effective suppression of follicle growth four weeks after the 2nd injection (n ¼ 6) were submitted to three consecutive ovum pick-up (OPU) sessions (weeks 10, 11, and 12) for in vitro embryo production (IVEP). Cumulus-oocyte complexes (COC) collected from slaughterhouse ovaries were used as controls for IVEP. COD cows with produced 25.0 ± 3.8 COC per OPU session with no apparent detrimental effect of anti-GnRH vaccine on oocyte developmental potential in vitro, i.e., we observed similar cleavage rate (P ¼ 0.5914) and greater blastocyst rate (P ¼ 0.0177) in immunized cows compared with COC from slaughterhouse controls. Finally, in the fourth experiment wave emergence and follicular dynamics after follicle ablation were compared between COD immunized cows with effective suppression of follicle growth and a subset (n ¼ 6) of the cycling, control group. No follicles grew beyond 4 mm diameter after follicle ablation in the COD immunized group, whereas a normal follicular wave emergence occurred in cycling controls. Antral follicle count was similar between cycling controls and COD immunized groups at 24 h and 96 h post-follicle ablation (P > 0.05), but greater in cycling controls at 48 h and 72 h post-follicle ablation (P < 0.05). In summary, our results suggest that active immunization against GnRH is effective to induce the regression of follicular cysts as well as increase the number of small follicles growing on the ovaries, in oocyte donors diagnosed with chronic COD, with no apparent negative effect on oocyte developmental potential in vitro.
650 $aBovino
650 $aDoença Animal
650 $aGado Nelore
653 $aFollicle growth suppression
653 $aGnRH vaccine
653 $aIn vitro embryo production
653 $aNelore breed
700 1 $aPEREIRA, N. E. S.
700 1 $aFARIA, O. A. C.
700 1 $aDIAS, L. R. O.
700 1 $aOLIVEIRA, E. R.
700 1 $aFERNANDES, C. A. C.
700 1 $aSIQUEIRA, L. G. B.
773 $tTheriogenology$gv. 172, p. 133-141, 2021.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
|
Data corrente: |
01/06/2020 |
|
Data da última atualização: |
17/11/2020 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 1 |
|
Autoria: |
BASSO, M. F.; ARRAES, F. B. M.; GROSSI-DE-SA, M.; MOREIRA, V. J. V.; ALVES-FERREIRA, M.; GROSSI-DE-SA, M. F. |
|
Afiliação: |
MARCOS FERNANDO BASSO; FABRÍCIO BARBOSA MONTEIRO ARRAES, UFRGS; MAÍRA GROSSI-DE-SA; VALDEIR JUNIO VAZ MOREIRA, UFRGS; MARCIO ALVES-FERREIRA, UFRJ; MARIA FATIMA GROSSI DE SA, Cenargen. |
|
Título: |
Insights into genetic and molecular elements for transgenic crop development. |
|
Ano de publicação: |
2020 |
|
Fonte/Imprenta: |
Frontiers in Plant Science, v. 11, article 509, 2020. |
|
DOI: |
10.3389/fpls.2020.00509 |
|
Idioma: |
Inglês |
|
Palavras-Chave: |
Minimal expression cassette; New biotechnological tools; Plant genetic transformation; T-DNA delivery. |
|
Thesaurus NAL: |
Tissue culture. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/213492/1/fpls-11-00509.pdf
|
|
Marc: |
LEADER 00769naa a2200241 a 4500
001 2122805
005 2020-11-17
008 2020 bl uuuu u00u1 u #d
024 7 $a10.3389/fpls.2020.00509$2DOI
100 1 $aBASSO, M. F.
245 $aInsights into genetic and molecular elements for transgenic crop development.$h[electronic resource]
260 $c2020
650 $aTissue culture
653 $aMinimal expression cassette
653 $aNew biotechnological tools
653 $aPlant genetic transformation
653 $aT-DNA delivery
700 1 $aARRAES, F. B. M.
700 1 $aGROSSI-DE-SA, M.
700 1 $aMOREIRA, V. J. V.
700 1 $aALVES-FERREIRA, M.
700 1 $aGROSSI-DE-SA, M. F.
773 $tFrontiers in Plant Science$gv. 11, article 509, 2020.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
