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| Registros recuperados : 34 | |
| 21. |  | MOTA, A. P. Z.; FERNANDEZ, D.; ARRAES, F. B. M.; PETITOT, A.-S.; MELO, B. P. de; SA, M. E. L. de; GRYNBERG, P.; SARAIVA, M. A. P.; GUIMARAES, P. M.; BRASILEIRO, A. C. M.; ALBUQUERQUE, E. V. S.; DANCHIN, E. G. J.; GROSSI-DE-SA, M. F. Evolutionarily conserved plant genes responsive to root-knot nematodes identified by comparative genomics. Molecular Genetics and Genomics, v. 295, p. 1063-1078, 2020.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 22. | | NORIEGA, D. D.; ARIAS, P. L.; BARBOSA, H. R.; ARRAES, F. B. M.; OSSA, G. A.; VILLEGAS, B.; COELHO, R. R.; ALBUQUERQUE, E. V. S.; TOGAWA, R. C.; GRYNBERG, P.; WANG, H.; VÉLEZ, A. M.; ARBOLEDA, J. W.; GROSSI-DE-SA, M. F.; SILVA, M. C. M.; VALENCIA-JIMÉNEZ, A. Transcriptome and gene expression analysis of three developmental stages of the coffee berry borer, Hypothenemus hampei. Scientific Reports, v.9, n. 1, article 12804, 2019.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 23. | | RIBEIRO, T. P.; ARRAES, F. B. M.; LOURENCO-TESSUTTI, I. T.; SILVA, M. S.; LISEI-DE-SÁ, M. E.; LUCENA, W. A.; MACEDO, L. L. P. de; LIMA, J. N.; AMORIM, R. M. S.; ARTICO, S.; ALVES-FERREIRA, M.; SILVA, M. C. M.; GROSSI-DE-SA, M. F. Transgenic cotton expressing Cry10Aa toxin confers high resistance to the cotton boll weevil. Plant Biotechnology Journal, v. 15, p. 997-1009, 2017. (Open Access).| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 24. | | OLIVEIRA, R. S. de; OLIVEIRA NETO, O. B.; MOURA, H. F. N.; MACEDO, L. L. P. de; ARRAES, F. B. M.; LUCENA, W. A.; LOURENCO TESSUTTI, I. T.; BARBOSA, A. A. de D.; SILVA, M. C. M. da; GROSSI DE SA, M. F. Transgenic cotton plants expressing Cry1Ia12 toxin confer resistance to fall armyworm (Spodoptera frugiperda) and cotton boll weevil (Anthonomus grandis). Frontiers in Plant Science, v. 7, article 165 , 2016.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 25. | | OLIVEIRA, R. S. de; OLIVEIRA NETO, O. B.; MOURA, H. F. N.; MACEDO, L. L. P. de; ARRAES, F. B M.; LUCENA, W. A.; LOURENCO, I. T.; BARBOSA, A. de D.; SILVA, M. C. M. da; SA, M. F. G. de. Transgenic cotton plants expressing Cry1la12 toxin confer resistance to fall armyworm (Spodoptera frugiperda) and cotton boll weevil (Anthonomus grandis). Frontiers in Plant Science, v. 7, Article 165, 2016.| Biblioteca(s): Embrapa Algodão. |
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| 26. | | ARRAES, F. B. M.; VASQUEZ, D. D. N.; TAHIR, M.; PINHEIRO, D. H.; FAHEEM, M.; FREITAS-ALVES, N. S.; MOREIRA-PINTO, C. E.; MOREIRA, V. J. V.; PAES-DE-MELO, B.; LISEI-DE-SA, M. E.; MORGANTE, C. V.; MOTA, A. P. Z.; LOURENCO, I. T.; TOGAWA, R. C.; GRYNBERG, P.; FRAGOSO, R. da R.; ALMEIDA-ENGLER, J. de; LARSEN, M. R.; GROSSI-DE-SA, M. F. Integrated omic approaches reveal molecular mechanisms of tolerance during soybean and meloidogyne incognita interactions. Plants, v. 11, 2744, 2022.| Biblioteca(s): Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| 27. | | BASSO, M. F.; COSTA, J. A.; RIBEIRO, T. P.; ARRAES, F. B. M.; LOURENCO, I. T.; MACEDO, A. F.; NEVES, M. R. das; NARDELI, S. M.; ARGE, L. W.; PEREZ, C. E. A.; SILVA, P. L. R.; MACEDO, L. L. P. de; LISEI-DE-SA, M. E.; AMORIM, R. M. S.; PINTO, E. R. de C.; SILVA, M. C. M. da; MORGANTE, C. V.; FLOH, E. I. S.; ALVES-FERREIRA, M.; SA, M. F. G. de. Overexpression of the CaHB12 transcription factor in cotton (Gossypium hirsutum) improves drought tolerance. Plant Physiology and Biochemistry, v. 165, p. 80-93, 2021. Na publicação: Isabela Tristan Lourenço-Tessutti; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| 28. | | BASSO, M. F.; LOURENCO, I. T.; MOREIRA‐PINTO, C. E.; MENDES, R. A. G.; PEREIRA, D. G.; GRANDIS, A.; MACEDO, L. L. P. de; MACEDO, A. F.; GOMES, A. C. M. M.; ARRAES, F. B. M.; TOGAWA, R. C.; COSTA, M. M. do C.; MARCELINO-GUIMARÃES, F. C.; SILVA, M. C. M. da; FLOH, E. I. S.; BUCKERIDGE, M. S.; ENGLER, J. de A.; SA, M. F. G. de. Overexpression of the GmEXPA1 gene reduces plant susceptibility to Meloidogyne incognita. Plant Cell Reports, v. 42, p. 137-152, 2023.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
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| 29. | | BASSO, M. F.; LOURENCO, I. T.; MOREIRA-PINTO, C. E.; MENDES, R. A. G.; PAES-DE-MELO, B.; NEVES, M. R. das; MACEDO, A. F.; FIGUEIREDO, V.; GRANDIS, A.; MACEDO, L. L. P. de; ARRAES, F. B. M.; COSTA, M. M. do C.; TOGAWA, R. C.; ENRICH-PRAST, A.; MARCELINO-GUIMARÃES, F. C.; GOMES, A. C. M. M.; SILVA, M. C. M. da; FLOH, E. I. S.; BUCKERIDGE, M. S.; ENGLER, J. de A.; SA, M. F. G. de. Overexpression of a soybean Globin (GmGlb1-1) gene reduces plant susceptibility to Meloidogyne incognita. Planta, v. 256, 83, 2022. 16 p. Na publicação: Isabela Tristan Lourenço-Tessutti; Leonardo Lima Pepino Macedo; Francismar Corrêa Marcelino-Guimaraes; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
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| 30. | | TÁVORA, F. T. P. K.; DINIZ, F. de A. dos S.; RÊGO-MACHADO, C. de M.; FREITAS, N. C.; ARRAES, F. B. M.; ANDRADE, E. C. de; FURTADO, L. L.; OSIRO, K. O.; SOUSA, N. L. de; CARDOSO, T. B.; MERTZ-HENNING, L. M.; OLIVEIRA, P. A. de; FEINGOLD, S. E.; HUNTER, W. B.; SA, M. F. G. de; KOBAYASHI, A. K.; NEPOMUCENO, A. L.; SANTIAGO, T. R.; MOLINARI, H. B. C. CRISPR/Cas- and Topical RNAi-Based Technologies for crop management and improvement: reviewing the risk assessment and challenges towards a more sustainable agriculture. Frontiers in Bioengineering and Biotechnology, v. 10, 2022. Article 913728.| Biblioteca(s): Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
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| 31. | | LISEI-DE-SÁ, M. e; RODRIGUES‑SILVA, P. L.; MORGANTE, C. V.; MELO, B. P. de; LOURENCO, I. T.; ARRAES, F. B. M.; SOUSA, J. P. A.; GALBIERI, R.; AMORIM, R. M. S.; LINS, C. B. J. de; MACEDO, L. L. P. de; MOREIRA, V. J.; FERREIRA, G. F.; RIBEIRO, T. P.; FRAGOSO, R. da R.; SILVA, M. C. M. da; ALMEIDA-ENGLER, J. de; SA, M. F. G. de. Pyramiding dsRNAs increases phytonematode tolerance in cotton plants. Planta, v. 254, 2021. Na publicação: Isabela T. Lourenço-Tessutti; Leonardo L. P. Macedo; Rodrigo R. Fragoso; Maria C. M. Silva; Maria F. Grossi-de-Sa.| Biblioteca(s): Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| 32. | | FREITAS‑ALVES, N. S.; MOREIRA‑PINTO, C. E.; ARRAES, F. B. M.; COSTA, L. S. de L.; ABREU, R. A. de; MOREIRA, V. J. V.; LOURENCO, I. T.; PINHEIRO, D. H.; LISEI‑DE‑SA, M. E.; PAES‑DE‑MELO, B.; PEREIRA, B. M.; GUIMARAES, P. M.; BRASILEIRO, A. C. M.; ALMEIDA‑ENGLER, J. de; SOCCOL, C. R.; MORGANTE, C. V.; BASSO, M. F.; SA, M. F. G. de. An ex vitro hairy root system from petioles of detached soybean leaves for in planta screening of target genes and CRISPR strategies associated with nematode bioassays. Planta, v. 259, 23, 2024. Na publicação: Isabela T. Lourenço‑Tessutti; Maria F. Grossi‑de‑Sa.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 33. | | FRAGOSO, R. da R.; ARRAES, F. B. M.; LOURENCO, I. T.; MIRANDA, V. J.; BASSO, M. F.; FERREIRA, A. V. J.; VIANA, A. A. B.; LINS, C. B. J.; LINS, P. C.; MOURA, S. M.; BATISTA, J. A. N.; SILVA, M. C. M. da; ENGLER, G.; MORGANTE, C. V.; LISEI-DE-SA, M. E.; VASQUES, R. M.; ALMEIDA-ENGLER, J. de; SA, M. F. G. de. Functional characterization of the pUceS8.3 promoter and its potential use for ectopic gene overexpression. Planta, v. 256, n. 4, 2022. Na publicação: Rodrigo Rocha Fragoso; Isabela Tristan Lourenço-Tessutti; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa.| Biblioteca(s): Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| 34. | | FELIPE, M. S. S.; ANDRADE, R. V.; ARRAES, F. B. M.; NICOLA, A. M.; MARANHÃO, A. Q.; TORRES, F. A. G.; SILVA-PEREIRA, I.; POÇAS-FONSECA, M. J.; CAMPOS, E. G.; MORAES, L. M. P.; ANDRADE, P. A.; TAVARES, A. H. F. P.; SILVA, S. S.; KYAW, C. M.; SOUZA, D. P.; PB GENOME NETWORK; PEREIRA, M.; JESUÍNO, R. S. A.; ANDRADE, E. V.; PARENTE, J. A.; OLIVEIRA, G. S.; BARBOSA, M. S.; MARTINS, N. F.; FACHIN, A. L.; CARDOSO, R. S.; PASSOS, G. A. S.; ALMEIDA, N. F.; WALTER, M. E. M. T.; SOARES, C. M. A.; CARVALHO, M. J. A.; BRÍGIDO, M. M. Transcriptional profiles of the human pathogenic fungus Paracoccidioides brasilienses in mycelium and yeast cells. JBC Papers, April 27, 2005. 19 p. JBC Papers in press. Published on 27 April, 2005 as manuscript M500625200.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 34 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
20/09/2022 |
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Data da última atualização: |
13/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
FRAGOSO, R. da R.; ARRAES, F. B. M.; LOURENCO, I. T.; MIRANDA, V. J.; BASSO, M. F.; FERREIRA, A. V. J.; VIANA, A. A. B.; LINS, C. B. J.; LINS, P. C.; MOURA, S. M.; BATISTA, J. A. N.; SILVA, M. C. M. da; ENGLER, G.; MORGANTE, C. V.; LISEI-DE-SA, M. E.; VASQUES, R. M.; ALMEIDA-ENGLER, J. de; SA, M. F. G. de. |
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Afiliação: |
RODRIGO DA ROCHA FRAGOSO, CNPAE; FABRICIO BARBOSA MONTEIRO ARRAES; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; VÍVIAN JESUS MIRANDA; MARCOS FERNANDO BASSO; ANDRÉ VINICIUS JÚLIO FERREIRA; ANTÔNIO AMÉRICO BARBOSA VIANA; CAMILA BARROZO JESUS LINS; PHILIPPE CASTRO LINS; STÉFANIE MENEZES MOURA; JOÃO AGUIAR NOGUEIRA BATISTA, Federal University of Minas Gerais; MARIA CRISTINA MATTAR DA SILVA, Cenargen; GILBERT ENGLER, Université Côte d'Azur, France; CAROLINA VIANNA MORGANTE, CPATSA; MARIA EUGÊNIA LISEI-DE-SA, Minas Gerais Agricultural Research Company; RAQUEL MEDEIROS VASQUES; JANICE DE ALMEIDA-ENGLER, Université Côte d'Azur, France; MARIA FATIMA GROSSI DE SA, Cenargen. |
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Título: |
Functional characterization of the pUceS8.3 promoter and its potential use for ectopic gene overexpression. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Planta, v. 256, n. 4, 2022. |
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DOI: |
https://doi.org/10.1007/s00425-022-03980-6 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Rodrigo Rocha Fragoso; Isabela Tristan Lourenço-Tessutti; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa. |
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Conteúdo: |
Soybean (Glycine max) is one of the most important agricultural commodities worldwide and a major protein and oil source. Herein, we identified the soybean ubiquitin-conjugating (E2) enzyme gene (GmUBC4; Glyma.18G216000), which is significantly upregulated in response to Anticarsia gemmatalis attack and Meloidogyne incognita-induced galls during plant parasitism by plant nematode. The GmUBC4 promoter sequence and its different modules were functionally characterized in silico and in planta using transgenic Arabidopsis thaliana and G. max lines. Its full-length transcriptional regulatory region (promoter and 5´-UTR sequences, named pUceS8.3 promoter) was able to drive higher levels of uidA (?-glucuronidase) gene expression in different tissues of transgenic A. thaliana lines compared to its three shortened modules and the p35SdAMV promoter. Notably, higher ?-glucuronidase (GUS) enzymatic activity was shown in M. incognita-induced giant cells when the full pUceS8.3 promoter drove the expression of this reporter gene. Furthermore, nematode-specific dsRNA molecules were successfully overexpressed under the control of the pUceS8.3 promoter in transgenic soybean lines. The RNAi gene construct used here was designed to post-transcriptionally downregulate the previously characterized pre-mRNA splicing factor genes from Heterodera glycines and M. incognita. A total of six transgenic soybean lines containing RNAi gene construct were selected for molecular characterization after infection with M. incognita pre-parasitic second-stage (ppJ2) nematodes. A strong reduction in the egg number produced by M. incognita after parasitism was observed in those transgenic soybean lines, ranging from 71 to 92% compared to wild-type control plants. The present data demonstrated that pUceS8.3 is a gene promoter capable of effectively driving dsRNA overexpression in nematode-induced giant cells of transgenic soybean lines and can be successfully applied as an important biotechnological asset to generate transgenic crops with improved resistance to root-knot nematodes as well as other pests. MenosSoybean (Glycine max) is one of the most important agricultural commodities worldwide and a major protein and oil source. Herein, we identified the soybean ubiquitin-conjugating (E2) enzyme gene (GmUBC4; Glyma.18G216000), which is significantly upregulated in response to Anticarsia gemmatalis attack and Meloidogyne incognita-induced galls during plant parasitism by plant nematode. The GmUBC4 promoter sequence and its different modules were functionally characterized in silico and in planta using transgenic Arabidopsis thaliana and G. max lines. Its full-length transcriptional regulatory region (promoter and 5´-UTR sequences, named pUceS8.3 promoter) was able to drive higher levels of uidA (?-glucuronidase) gene expression in different tissues of transgenic A. thaliana lines compared to its three shortened modules and the p35SdAMV promoter. Notably, higher ?-glucuronidase (GUS) enzymatic activity was shown in M. incognita-induced giant cells when the full pUceS8.3 promoter drove the expression of this reporter gene. Furthermore, nematode-specific dsRNA molecules were successfully overexpressed under the control of the pUceS8.3 promoter in transgenic soybean lines. The RNAi gene construct used here was designed to post-transcriptionally downregulate the previously characterized pre-mRNA splicing factor genes from Heterodera glycines and M. incognita. A total of six transgenic soybean lines containing RNAi gene construct were selected for molecular characterization after infect... Mostrar Tudo |
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Palavras-Chave: |
GmUBC4 promoter/gene; Ubiquitin-conjugating (E2) enzyme gene promoter. |
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Thesagro: |
Anticarsia Gemmatalis; Glycine Max; Meloidogyne Incognita; Soja. |
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Thesaurus NAL: |
RNA interference; Soybeans. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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Marc: |
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001 2148961
005 2023-01-13
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100 1 $aFRAGOSO, R. da R.
245 $aFunctional characterization of the pUceS8.3 promoter and its potential use for ectopic gene overexpression.$h[electronic resource]
260 $c2022
500 $aNa publicação: Rodrigo Rocha Fragoso; Isabela Tristan Lourenço-Tessutti; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa.
520 $aSoybean (Glycine max) is one of the most important agricultural commodities worldwide and a major protein and oil source. Herein, we identified the soybean ubiquitin-conjugating (E2) enzyme gene (GmUBC4; Glyma.18G216000), which is significantly upregulated in response to Anticarsia gemmatalis attack and Meloidogyne incognita-induced galls during plant parasitism by plant nematode. The GmUBC4 promoter sequence and its different modules were functionally characterized in silico and in planta using transgenic Arabidopsis thaliana and G. max lines. Its full-length transcriptional regulatory region (promoter and 5´-UTR sequences, named pUceS8.3 promoter) was able to drive higher levels of uidA (?-glucuronidase) gene expression in different tissues of transgenic A. thaliana lines compared to its three shortened modules and the p35SdAMV promoter. Notably, higher ?-glucuronidase (GUS) enzymatic activity was shown in M. incognita-induced giant cells when the full pUceS8.3 promoter drove the expression of this reporter gene. Furthermore, nematode-specific dsRNA molecules were successfully overexpressed under the control of the pUceS8.3 promoter in transgenic soybean lines. The RNAi gene construct used here was designed to post-transcriptionally downregulate the previously characterized pre-mRNA splicing factor genes from Heterodera glycines and M. incognita. A total of six transgenic soybean lines containing RNAi gene construct were selected for molecular characterization after infection with M. incognita pre-parasitic second-stage (ppJ2) nematodes. A strong reduction in the egg number produced by M. incognita after parasitism was observed in those transgenic soybean lines, ranging from 71 to 92% compared to wild-type control plants. The present data demonstrated that pUceS8.3 is a gene promoter capable of effectively driving dsRNA overexpression in nematode-induced giant cells of transgenic soybean lines and can be successfully applied as an important biotechnological asset to generate transgenic crops with improved resistance to root-knot nematodes as well as other pests.
650 $aRNA interference
650 $aSoybeans
650 $aAnticarsia Gemmatalis
650 $aGlycine Max
650 $aMeloidogyne Incognita
650 $aSoja
653 $aGmUBC4 promoter/gene
653 $aUbiquitin-conjugating (E2) enzyme gene promoter
700 1 $aARRAES, F. B. M.
700 1 $aLOURENCO, I. T.
700 1 $aMIRANDA, V. J.
700 1 $aBASSO, M. F.
700 1 $aFERREIRA, A. V. J.
700 1 $aVIANA, A. A. B.
700 1 $aLINS, C. B. J.
700 1 $aLINS, P. C.
700 1 $aMOURA, S. M.
700 1 $aBATISTA, J. A. N.
700 1 $aSILVA, M. C. M. da
700 1 $aENGLER, G.
700 1 $aMORGANTE, C. V.
700 1 $aLISEI-DE-SA, M. E.
700 1 $aVASQUES, R. M.
700 1 $aALMEIDA-ENGLER, J. de
700 1 $aSA, M. F. G. de
773 $tPlanta$gv. 256, n. 4, 2022.
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