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Registros recuperados : 34 | |
21. | | OLIVEIRA, R. S. de; OLIVEIRA NETO, O. B.; MOURA, H. F. N.; MACEDO, L. L. P. de; ARRAES, F. B. M.; LUCENA, W. A.; LOURENCO TESSUTTI, I. T.; BARBOSA, A. A. de D.; SILVA, M. C. M. da; GROSSI DE SA, M. F. Transgenic cotton plants expressing Cry1Ia12 toxin confer resistance to fall armyworm (Spodoptera frugiperda) and cotton boll weevil (Anthonomus grandis). Frontiers in Plant Science, v. 7, article 165 , 2016. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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22. | | OLIVEIRA, R. S. de; OLIVEIRA NETO, O. B.; MOURA, H. F. N.; MACEDO, L. L. P. de; ARRAES, F. B M.; LUCENA, W. A.; LOURENCO, I. T.; BARBOSA, A. de D.; SILVA, M. C. M. da; SA, M. F. G. de. Transgenic cotton plants expressing Cry1la12 toxin confer resistance to fall armyworm (Spodoptera frugiperda) and cotton boll weevil (Anthonomus grandis). Frontiers in Plant Science, v. 7, Article 165, 2016. Biblioteca(s): Embrapa Algodão. |
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23. | | COSTA, L. S. de L.; ALVES, N. S. de F.; PINTO, C. E. M.; FLORENTINO, L. H.; MELO, B. P. de; MOREIRA, V. J. V.; SÁ, M. E. L. de; ARRAES, F. B. M.; RECH FILHO, E. L.; MORGANTE, C. V.; SA, M. F. G. de. Depleção do quadro de leitura upstream como nova estratégia para manipular a tradução de gmpr10 usando crispr/cas9 para aumentar a tolerância da soja a fitonematoides. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 8, 2023, Florianópolis, SC. Anais... Florianópolis: SBG, 2023. p. 131. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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24. | | COSTA, L. S. de L.; ALVES, N. S. de F.; PINTO, C. E. M.; FLORENTINO, L. H.; MELO, B. P. de; MOREIRA, V. J. V.; SÁ, M. E. L. de; ARRAES, F. B. M.; RECH FILHO, E. L.; MORGANTE, C. V.; SA, M. F. G. de. Depleção do quadro de leitura upstream como nova estratégia para manipular a tradução de gmpr10 usando crispr/cas9 para aumentar a tolerância da soja a fitonematoides. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 8, 2023, Florianópolis, SC. Anais... Florianópolis: SBG, 2023. p. 131. Biblioteca(s): Embrapa Semiárido. |
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25. | | FIRMINO, A. A. P.; PINHEIRO, D. H.; MOREIRA-PINTO, C. E.; ANTONINO, J. D.; MACEDO, L. L. P.; MARTINS-DE-SA, D.; ARRAES, F. B. M.; COELHO, R. R.; FONSECA, F. C. de A.; SILVA, M. C. M.; ENGLER, J. de A.; SILVA, M. S.; LOURENÇO-TESSUTTI, I. T.; TERRA, W. R.; GROSSI-DE-SA, M. F. RNAi-mediated suppression of Laccase2 impairs cuticle tanning and molting in the cotton boll weevil (Anthonomus grandis). Frontiers in Physiology, v. 11, article 591569, 2020. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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26. | | FRAGOSO, R. da R.; ARRAES, F. B. M.; LOURENCO, I. T.; MIRANDA, V. J.; BASSO, M. F.; FERREIRA, A. V. J.; VIANA, A. A. B.; LINS, C. B. J.; LINS, P. C.; MOURA, S. M.; BATISTA, J. A. N.; SILVA, M. C. M. da; ENGLER, G.; MORGANTE, C. V.; LISEI-DE-SA, M. E.; VASQUES, R. M.; ALMEIDA-ENGLER, J. de; SA, M. F. G. de. Functional characterization of the pUceS8.3 promoter and its potential use for ectopic gene overexpression. Planta, v. 256, n. 4, 2022. Na publicação: Rodrigo Rocha Fragoso; Isabela Tristan Lourenço-Tessutti; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa. Biblioteca(s): Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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27. | | FREITAS‑ALVES, N. S.; MOREIRA‑PINTO, C. E.; ARRAES, F. B. M.; COSTA, L. S. de L.; ABREU, R. A. de; MOREIRA, V. J. V.; LOURENCO, I. T.; PINHEIRO, D. H.; LISEI‑DE‑SA, M. E.; PAES‑DE‑MELO, B.; PEREIRA, B. M.; GUIMARAES, P. M.; BRASILEIRO, A. C. M.; ALMEIDA‑ENGLER, J. de; SOCCOL, C. R.; MORGANTE, C. V.; BASSO, M. F.; SA, M. F. G. de. An ex vitro hairy root system from petioles of detached soybean leaves for in planta screening of target genes and CRISPR strategies associated with nematode bioassays. Planta, v. 259, 23, 2024. Na publicação: Isabela T. Lourenço‑Tessutti; Maria F. Grossi‑de‑Sa. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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28. | | BASSO, M. F.; COSTA, J. A.; RIBEIRO, T. P.; ARRAES, F. B. M.; LOURENCO, I. T.; MACEDO, A. F.; NEVES, M. R. das; NARDELI, S. M.; ARGE, L. W.; PEREZ, C. E. A.; SILVA, P. L. R.; MACEDO, L. L. P. de; LISEI-DE-SA, M. E.; AMORIM, R. M. S.; PINTO, E. R. de C.; SILVA, M. C. M. da; MORGANTE, C. V.; FLOH, E. I. S.; ALVES-FERREIRA, M.; SA, M. F. G. de. Overexpression of the CaHB12 transcription factor in cotton (Gossypium hirsutum) improves drought tolerance. Plant Physiology and Biochemistry, v. 165, p. 80-93, 2021. Na publicação: Isabela Tristan Lourenço-Tessutti; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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29. | | BASSO, M. F.; LOURENCO, I. T.; MOREIRA‐PINTO, C. E.; MENDES, R. A. G.; PEREIRA, D. G.; GRANDIS, A.; MACEDO, L. L. P. de; MACEDO, A. F.; GOMES, A. C. M. M.; ARRAES, F. B. M.; TOGAWA, R. C.; COSTA, M. M. do C.; MARCELINO-GUIMARÃES, F. C.; SILVA, M. C. M. da; FLOH, E. I. S.; BUCKERIDGE, M. S.; ENGLER, J. de A.; SA, M. F. G. de. Overexpression of the GmEXPA1 gene reduces plant susceptibility to Meloidogyne incognita. Plant Cell Reports, v. 42, p. 137-152, 2023. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
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30. | | BASSO, M. F.; LOURENCO, I. T.; MOREIRA-PINTO, C. E.; MENDES, R. A. G.; PAES-DE-MELO, B.; NEVES, M. R. das; MACEDO, A. F.; FIGUEIREDO, V.; GRANDIS, A.; MACEDO, L. L. P. de; ARRAES, F. B. M.; COSTA, M. M. do C.; TOGAWA, R. C.; ENRICH-PRAST, A.; MARCELINO-GUIMARÃES, F. C.; GOMES, A. C. M. M.; SILVA, M. C. M. da; FLOH, E. I. S.; BUCKERIDGE, M. S.; ENGLER, J. de A.; SA, M. F. G. de. Overexpression of a soybean Globin (GmGlb1-1) gene reduces plant susceptibility to Meloidogyne incognita. Planta, v. 256, 83, 2022. 16 p. Na publicação: Isabela Tristan Lourenço-Tessutti; Leonardo Lima Pepino Macedo; Francismar Corrêa Marcelino-Guimaraes; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
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31. | | LISEI-DE-SÁ, M. e; RODRIGUES‑SILVA, P. L.; MORGANTE, C. V.; MELO, B. P. de; LOURENCO, I. T.; ARRAES, F. B. M.; SOUSA, J. P. A.; GALBIERI, R.; AMORIM, R. M. S.; LINS, C. B. J. de; MACEDO, L. L. P. de; MOREIRA, V. J.; FERREIRA, G. F.; RIBEIRO, T. P.; FRAGOSO, R. da R.; SILVA, M. C. M. da; ALMEIDA-ENGLER, J. de; SA, M. F. G. de. Pyramiding dsRNAs increases phytonematode tolerance in cotton plants. Planta, v. 254, 2021. Na publicação: Isabela T. Lourenço-Tessutti; Leonardo L. P. Macedo; Rodrigo R. Fragoso; Maria C. M. Silva; Maria F. Grossi-de-Sa. Biblioteca(s): Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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32. | | ARRAES, F. B. M.; VASQUEZ, D. D. N.; TAHIR, M.; PINHEIRO, D. H.; FAHEEM, M.; FREITAS-ALVES, N. S.; MOREIRA-PINTO, C. E.; MOREIRA, V. J. V.; PAES-DE-MELO, B.; LISEI-DE-SA, M. E.; MORGANTE, C. V.; MOTA, A. P. Z.; LOURENCO, I. T.; TOGAWA, R. C.; GRYNBERG, P.; FRAGOSO, R. da R.; ALMEIDA-ENGLER, J. de; LARSEN, M. R.; GROSSI-DE-SA, M. F. Integrated omic approaches reveal molecular mechanisms of tolerance during soybean and meloidogyne incognita interactions. Plants, v. 11, 2744, 2022. Biblioteca(s): Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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33. | | TÁVORA, F. T. P. K.; DINIZ, F. de A. dos S.; RÊGO-MACHADO, C. de M.; FREITAS, N. C.; ARRAES, F. B. M.; ANDRADE, E. C. de; FURTADO, L. L.; OSIRO, K. O.; SOUSA, N. L. de; CARDOSO, T. B.; MERTZ-HENNING, L. M.; OLIVEIRA, P. A. de; FEINGOLD, S. E.; HUNTER, W. B.; SA, M. F. G. de; KOBAYASHI, A. K.; NEPOMUCENO, A. L.; SANTIAGO, T. R.; MOLINARI, H. B. C. CRISPR/Cas- and Topical RNAi-Based Technologies for crop management and improvement: reviewing the risk assessment and challenges towards a more sustainable agriculture. Frontiers in Bioengineering and Biotechnology, v. 10, 2022. Article 913728. Biblioteca(s): Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
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34. | | FELIPE, M. S. S.; ANDRADE, R. V.; ARRAES, F. B. M.; NICOLA, A. M.; MARANHÃO, A. Q.; TORRES, F. A. G.; SILVA-PEREIRA, I.; POÇAS-FONSECA, M. J.; CAMPOS, E. G.; MORAES, L. M. P.; ANDRADE, P. A.; TAVARES, A. H. F. P.; SILVA, S. S.; KYAW, C. M.; SOUZA, D. P.; PB GENOME NETWORK; PEREIRA, M.; JESUÍNO, R. S. A.; ANDRADE, E. V.; PARENTE, J. A.; OLIVEIRA, G. S.; BARBOSA, M. S.; MARTINS, N. F.; FACHIN, A. L.; CARDOSO, R. S.; PASSOS, G. A. S.; ALMEIDA, N. F.; WALTER, M. E. M. T.; SOARES, C. M. A.; CARVALHO, M. J. A.; BRÍGIDO, M. M. Transcriptional profiles of the human pathogenic fungus Paracoccidioides brasilienses in mycelium and yeast cells. JBC Papers, April 27, 2005. 19 p. JBC Papers in press. Published on 27 April, 2005 as manuscript M500625200. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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Registros recuperados : 34 | |
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Registro Completo
Biblioteca(s): |
Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
Data corrente: |
23/11/2022 |
Data da última atualização: |
08/12/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 4 |
Autoria: |
ARRAES, F. B. M.; VASQUEZ, D. D. N.; TAHIR, M.; PINHEIRO, D. H.; FAHEEM, M.; FREITAS-ALVES, N. S.; MOREIRA-PINTO, C. E.; MOREIRA, V. J. V.; PAES-DE-MELO, B.; LISEI-DE-SA, M. E.; MORGANTE, C. V.; MOTA, A. P. Z.; LOURENCO, I. T.; TOGAWA, R. C.; GRYNBERG, P.; FRAGOSO, R. da R.; ALMEIDA-ENGLER, J. de; LARSEN, M. R.; GROSSI-DE-SA, M. F. |
Afiliação: |
FABRICIO B. M. ARRAES, FEDERAL UNIVERSITY OF RIO GRANDE DO SUL; DANIEL D. N. VASQUEZ, FEDERAL UNIVERSITY OF RIO GRANDE DO SUL; MUHAMMED TAHIR, UNIVERSITY OF SOUTHERN DENMARK; DANIELE H. PINHEIRO, NATIONAL INSTITUTE OF SCIENCE AND TECHNOLOGY; MUHAMMED FAHEEM, NATIONAL UNIVERSITY OF MEDICAL SCIENCES, PAKISTAN; NAYARA S. FREITAS-ALVES, FEDERAL UNIVERSITY OF PARANÁ; CLÍDIA E. MOREIRA-PINTO, CNPAE; VALDEIR J. V. MOREIRA, UNIVERSITY OF BRASÍLIA; BRUNO PAES-DE-MELO, CNPAE; MARIA E. LISEI-DE-SA, MINAS GERAIS AGRICULTURAL RESEARCH COMPANY; CAROLINA VIANNA MORGANTE, CPATSA; ANA P. Z. MOTA, INRAE; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; ROBERTO COITI TOGAWA, Cenargen; PRISCILA GRYNBERG, Cenargen; RODRIGO DA ROCHA FRAGOSO, CNPAE; JANICE DE ALMEIDA-ENGLER, INRAE; MARTIN R. LARSEN, UNIVERSITY OF SOUTHERN DENMARK; MARIA FATIMA GROSSI-DE-SA, Cenargen. |
Título: |
Integrated omic approaches reveal molecular mechanisms of tolerance during soybean and meloidogyne incognita interactions. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Plants, v. 11, 2744, 2022. |
ISSN: |
2223-7747 |
DOI: |
https:// doi.org/10.3390/plants11202744 |
Idioma: |
Inglês |
Conteúdo: |
The root-knot nematode (RKN), Meloidogyne incognita, is a devastating soybean pathogen worldwide. The use of resistant cultivars is the most effective method to prevent economic losses caused by RKNs. To elucidate the mechanisms involved in resistance to RKN, we determined the proteome and transcriptome profiles from roots of susceptible (BRS133) and highly tolerant (PI595099) Glycine max genotypes 4, 12, and 30 days after RKN infestation. After in silico analysis, we described major defense molecules and mechanisms considered constitutive responses to nematodeinfestation, such as mTOR, PI3K-Akt, relaxin, and thermogenesis. The integrated data allowed us to identify protein families and metabolic pathways exclusively regulated in tolerant soybean genotypes. Among them, we highlighted the phenylpropanoid pathway as an early, robust, and systemic defense process capable of controlling M. incognita reproduction. Associated with this metabolic pathway, 29 differentially expressed genes encoding 11 different enzymes were identified, mainly from the flavonoid and derivative pathways. Based on differential expression in transcriptomic and proteomic data, as well as in the expression profile by RT?qPCR, and previous studies, we selected and overexpressed the GmPR10 gene in transgenic tobacco to assess its protective effect against M. incognita. Transgenic plants of the T2 generation showed up to 58% reduction in the M. incognita reproduction factor. Finally, data suggest that GmPR10 overexpression can be effective against the plant parasitic nematodeM. incognita, but its mechanism of action remains unclear. These findings will help develop new engineered soybean genotypes with higher performance in response to RKN infections. MenosThe root-knot nematode (RKN), Meloidogyne incognita, is a devastating soybean pathogen worldwide. The use of resistant cultivars is the most effective method to prevent economic losses caused by RKNs. To elucidate the mechanisms involved in resistance to RKN, we determined the proteome and transcriptome profiles from roots of susceptible (BRS133) and highly tolerant (PI595099) Glycine max genotypes 4, 12, and 30 days after RKN infestation. After in silico analysis, we described major defense molecules and mechanisms considered constitutive responses to nematodeinfestation, such as mTOR, PI3K-Akt, relaxin, and thermogenesis. The integrated data allowed us to identify protein families and metabolic pathways exclusively regulated in tolerant soybean genotypes. Among them, we highlighted the phenylpropanoid pathway as an early, robust, and systemic defense process capable of controlling M. incognita reproduction. Associated with this metabolic pathway, 29 differentially expressed genes encoding 11 different enzymes were identified, mainly from the flavonoid and derivative pathways. Based on differential expression in transcriptomic and proteomic data, as well as in the expression profile by RT?qPCR, and previous studies, we selected and overexpressed the GmPR10 gene in transgenic tobacco to assess its protective effect against M. incognita. Transgenic plants of the T2 generation showed up to 58% reduction in the M. incognita reproduction factor. Finally, data suggest that GmPR10... Mostrar Tudo |
Palavras-Chave: |
Differential expression; Root-knot nematode. |
Thesagro: |
Glycine Max; Meloidogyne Incognita; Soja. |
Thesaurus NAL: |
Phenylpropanoids; Proteome; Transcriptome. |
Categoria do assunto: |
-- G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148619/1/Integrated-omic-approaches.pdf
|
Marc: |
LEADER 03069naa a2200457 a 4500 001 2148619 005 2023-12-08 008 2022 bl uuuu u00u1 u #d 022 $a2223-7747 024 7 $ahttps:// doi.org/10.3390/plants11202744$2DOI 100 1 $aARRAES, F. B. M. 245 $aIntegrated omic approaches reveal molecular mechanisms of tolerance during soybean and meloidogyne incognita interactions.$h[electronic resource] 260 $c2022 520 $aThe root-knot nematode (RKN), Meloidogyne incognita, is a devastating soybean pathogen worldwide. The use of resistant cultivars is the most effective method to prevent economic losses caused by RKNs. To elucidate the mechanisms involved in resistance to RKN, we determined the proteome and transcriptome profiles from roots of susceptible (BRS133) and highly tolerant (PI595099) Glycine max genotypes 4, 12, and 30 days after RKN infestation. After in silico analysis, we described major defense molecules and mechanisms considered constitutive responses to nematodeinfestation, such as mTOR, PI3K-Akt, relaxin, and thermogenesis. The integrated data allowed us to identify protein families and metabolic pathways exclusively regulated in tolerant soybean genotypes. Among them, we highlighted the phenylpropanoid pathway as an early, robust, and systemic defense process capable of controlling M. incognita reproduction. Associated with this metabolic pathway, 29 differentially expressed genes encoding 11 different enzymes were identified, mainly from the flavonoid and derivative pathways. Based on differential expression in transcriptomic and proteomic data, as well as in the expression profile by RT?qPCR, and previous studies, we selected and overexpressed the GmPR10 gene in transgenic tobacco to assess its protective effect against M. incognita. Transgenic plants of the T2 generation showed up to 58% reduction in the M. incognita reproduction factor. Finally, data suggest that GmPR10 overexpression can be effective against the plant parasitic nematodeM. incognita, but its mechanism of action remains unclear. These findings will help develop new engineered soybean genotypes with higher performance in response to RKN infections. 650 $aPhenylpropanoids 650 $aProteome 650 $aTranscriptome 650 $aGlycine Max 650 $aMeloidogyne Incognita 650 $aSoja 653 $aDifferential expression 653 $aRoot-knot nematode 700 1 $aVASQUEZ, D. D. N. 700 1 $aTAHIR, M. 700 1 $aPINHEIRO, D. H. 700 1 $aFAHEEM, M. 700 1 $aFREITAS-ALVES, N. S. 700 1 $aMOREIRA-PINTO, C. E. 700 1 $aMOREIRA, V. J. V. 700 1 $aPAES-DE-MELO, B. 700 1 $aLISEI-DE-SA, M. E. 700 1 $aMORGANTE, C. V. 700 1 $aMOTA, A. P. Z. 700 1 $aLOURENCO, I. T. 700 1 $aTOGAWA, R. C. 700 1 $aGRYNBERG, P. 700 1 $aFRAGOSO, R. da R. 700 1 $aALMEIDA-ENGLER, J. de 700 1 $aLARSEN, M. R. 700 1 $aGROSSI-DE-SA, M. F. 773 $tPlants$gv. 11, 2744, 2022.
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Embrapa Agroenergia (CNPAE) |
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