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Registro Completo |
Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
03/01/1994 |
Data da última atualização: |
03/01/1994 |
Autoria: |
MARCELINO, G. |
Título: |
Descentralizacao em ciencia e tecnologia. |
Ano de publicação: |
1985 |
Fonte/Imprenta: |
Sao Paulo: Cortez/CNPq, 1985. |
Páginas: |
226p. |
Descrição Física: |
il. |
Idioma: |
Português |
Conteúdo: |
Ciencia e tecnologia no Brasil. Sistemas e a hierarquizacao de decisoes. O contexto. Metodologia e levantamento inicial. A pesquisa de acompanhamento. O modelo proposto. Conclusoes e recomendacoes. |
Palavras-Chave: |
Ciencia; Descentralizacao; Descentralization; Science. |
Thesagro: |
Tecnologia. |
Thesaurus Nal: |
technology. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00665nam a2200193 a 4500 001 1385350 005 1994-01-03 008 1985 bl uuuu 00u1 u #d 100 1 $aMARCELINO, G. 245 $aDescentralizacao em ciencia e tecnologia. 260 $aSao Paulo: Cortez/CNPq$c1985 300 $a226p.$cil. 520 $aCiencia e tecnologia no Brasil. Sistemas e a hierarquizacao de decisoes. O contexto. Metodologia e levantamento inicial. A pesquisa de acompanhamento. O modelo proposto. Conclusoes e recomendacoes. 650 $atechnology 650 $aTecnologia 653 $aCiencia 653 $aDescentralizacao 653 $aDescentralization 653 $aScience
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Trigo. |
Data corrente: |
03/01/2018 |
Data da última atualização: |
09/08/2022 |
Tipo da produção científica: |
Nota Técnica/Nota Científica |
Circulação/Nível: |
A - 1 |
Autoria: |
LIMA, J. O.; PEREIRA, J. F.; ARAÚJO, R. F. de; QUEIROZ, M. V. de. |
Afiliação: |
JULIANA OLIVEIRA LIMA, UNIVERSIDADE FEDERAL DE VIÇOSA; JORGE FERNANDO PEREIRA, CNPGL; ELZA FERNANDES DE ARAÚJO, UNIVERSIDADE FEDERAL DE VIÇOSA; MARISA VIEIRA DE QUEIROZ, UNIVERSIDADE FEDERAL DE VIÇOSA. |
Título: |
Pectin lyase overproduction by Penicillium griseoroseum mutants resistant to catabolite repression. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Brazilian Journal of Microbiology, São Paulo, v. 48, n. 3, p. 602-606, 2017. |
DOI: |
https://doi.org/10.1016/j.bjm.2016.12.009 |
Idioma: |
Inglês |
Conteúdo: |
Expression of pectinolytic genes is regulated by catabolic repression limiting the productionof pectin lyase (PL) if the natural inducer, pectin, is missing from the growth medium. Here,we report the isolation of Penicillium griseoroseum mutants resistant to 2-deoxy-d-glucose(DG) that show resistance to catabolite repression and overproduce PL. Three spontaneousand nine UV-induced mutants were obtained. Some mutants produced sectors (segmentsmorphologically different) that were also studied. The mutants were analyzed for pectinasesproduction on pectinase-agar plates and five mutants and two sectors showing larger clear-ing zones than the wild type were selected for quantitative assay. Although PL productionhigher than the wild type has been found, phenotype instability was observed for most ofthe mutants and, after transfers to nonselective medium, the DG resistance was no longerpresent. Only mutants M03 and M04 were stable maintaining the DG-resistance phenotype.When growing for 120 h in liquid medium containing glucose with or without pectin, bothmutants showed higher PL production. In the presence of glucose as sole carbon source, themutant M03 produced 7.8-fold more PL than the wild type. Due its phenotypic stability andPL overproduction, the mutant M03 presents potential for industrial applications. |
Palavras-Chave: |
Pectinolytic complex; Spontaneous mutant; Ultraviolet mutagenesis. |
Thesagro: |
Microbiologia; Mutação. |
Thesaurus NAL: |
Microbiology; Mutation. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1084275/1/Pectin-lyase-overproduction-by-Penicillium-griseoroseum-mutants.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/170232/1/ID44256-2017v48n4p602BrazJMicrobiol.pdf
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Marc: |
LEADER 02130naa a2200253 a 4500 001 2084275 005 2022-08-09 008 2017 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.bjm.2016.12.009$2DOI 100 1 $aLIMA, J. O. 245 $aPectin lyase overproduction by Penicillium griseoroseum mutants resistant to catabolite repression.$h[electronic resource] 260 $c2017 520 $aExpression of pectinolytic genes is regulated by catabolic repression limiting the productionof pectin lyase (PL) if the natural inducer, pectin, is missing from the growth medium. Here,we report the isolation of Penicillium griseoroseum mutants resistant to 2-deoxy-d-glucose(DG) that show resistance to catabolite repression and overproduce PL. Three spontaneousand nine UV-induced mutants were obtained. Some mutants produced sectors (segmentsmorphologically different) that were also studied. The mutants were analyzed for pectinasesproduction on pectinase-agar plates and five mutants and two sectors showing larger clear-ing zones than the wild type were selected for quantitative assay. Although PL productionhigher than the wild type has been found, phenotype instability was observed for most ofthe mutants and, after transfers to nonselective medium, the DG resistance was no longerpresent. Only mutants M03 and M04 were stable maintaining the DG-resistance phenotype.When growing for 120 h in liquid medium containing glucose with or without pectin, bothmutants showed higher PL production. In the presence of glucose as sole carbon source, themutant M03 produced 7.8-fold more PL than the wild type. Due its phenotypic stability andPL overproduction, the mutant M03 presents potential for industrial applications. 650 $aMicrobiology 650 $aMutation 650 $aMicrobiologia 650 $aMutação 653 $aPectinolytic complex 653 $aSpontaneous mutant 653 $aUltraviolet mutagenesis 700 1 $aPEREIRA, J. F. 700 1 $aARAÚJO, R. F. de 700 1 $aQUEIROZ, M. V. de 773 $tBrazilian Journal of Microbiology, São Paulo$gv. 48, n. 3, p. 602-606, 2017.
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Embrapa Gado de Leite (CNPGL) |
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