Data/hora: 05/06/2024 18:07:32

Biblioteca(s): | Embrapa Semiárido.
Data corrente: | 14/09/2022
Data da última atualização: | 14/09/2022
Autoria: | ORNELLAS, F. L. S.; SOUSA, A. O. de; PIROVANI, C. P.; ARAÚJO, M. do N; COSTA, D. S. da; DANTAS, B. F.; BARBOSA, R. M.
Afiliação: | FÁBIO LUIZ SANTOS ORNELLAS, Universidade Estadual de Santa Cruz; AURIZANGELA OLIVEIRA DE SOUSA, Universidade Federal do Oeste da Bahia; CARLOS PRIMINHO PIROVANI, Universidade Estadual de Santa Cruz; MARCELO DO NASCIMENTO ARAÚJO; DENIS SANTIAGO DA COSTA, Instituto Federal de Mato Grosso do Sul; BARBARA FRANCA DANTAS, CPATSA; RAFAEL MARANI BARBOSA, Universidade Estadual de Santa Cruz.
Título: | Gene expression, biochemical and physiological activities in evaluating melon seed vigor through ethanol release.
Ano de publicação: | 2020
Fonte/Imprenta: | Scientia Horticulturae, v. 261, 108884, 2020.
DOI: | https://doi.org/10.1016/j.scienta.2019.108884
Idioma: | Inglês
Conteúdo: | The aim of the present study was to establish parameters to evaluate melon seeds vigor by ethanol release test using a modified ethylometer and correlate with physiological seed quality, gene expression and enzyme activities alterations. To perform this trial, six seeds lots of yellow melon were submitted to determinations such as moisture content, germination assay and vigor tests. 25 and 50 seeds soaked with 0.5 mL of distilled water and tested after 1, 2, 3, 4, 5, 6 and 24 h at 40 °C. After 0, 6 and 24 h of imbibition, the high and low vigor lots were evaluated for the following enzyme activity: superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPX), ascorbate peroxidase (APX) and alcoholic dehydrogenase (ADH). Accumulation of ADH protein was verified using Western Blotting hybridization technique with the primary Anti-ADH antibody. The release of ethanol in 25 seeds, soaked in 0.5 mL of distilled water for 6 h, proved efficient for vigor evaluation and can be related to other traditionally used tests. Enzyme activity varied according to the vigor of the seed lot. For the protein profile, there was variation in the accumulation of proteins of specific molecular weights throughout the soaking periods. There was also variation in expression according to accumulation of ADH protein. The biochemical and molecular tests corroborate with the establishment of the ethanol test as an additional tool to evaluate seed vigor.
Palavras-Chave: | Etilômetro; Vigor de sementes de melão.
Thesagro: | Cucumis Melo; Enzima; Germinação; Melão; Semente.
Thesaurus Nal: | Enzymes; Seed germination.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1146419/1/Gene-expression-biochemical-and-physiological-2020.pdf
Registro original: | Embrapa Semiárido (CPATSA)
Exemplares: | CPATSA - (60025-1) - UPC - AP - DD -  -  -  - DIS