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| Registros recuperados : 168 | |
| 121. |  | PASSOS, S. R.; CORREIA, M. E. F.; SOARES, L. H. de B.; ARAUJO, J. L. S. de; RUMJANEK, N. G.; XAVIER, G. R. Exploração do potencial biotecnológico da microbiota associada ao trato intestinal de Trigoniulus corallinus (Diplopoda). In: SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 10., 18 a 24 de outubro de 2010, Seropédica. Ciência para o Desenvolvimento Sustentável: anais... Seropédica: Embrapa Agrobiologia, 2010.| Biblioteca(s): Embrapa Agrobiologia. |
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| 122. | | SILVA, H. A. P da; GALISA, P. de S.; OLIVEIRA, R. S. da S.; VIDAL, M. S.; ARAUJO, J. L. S. de. Expressão gênica induzida por estresses abióticos em nódulos de feijão-caupi. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 47, n. 6, p. 797-807, jun. 2012. Titulo em inglês: Gene expression induced by abiotic stress in cowpea nodules.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 123. | | SILVA, H. A. P. DA.; GALISA, P. DE S.; OLIVEIRA, R. S. DA; VIDAL, M. S.; ARAUJO, J. L. S. de. Expressão gênica induzida por estresses abióticos em nódulos de feijão-caupi. Pesquisa Agropecuária Brasileira, v. 47, n. 6, p. 797-807, abr./jun., 2012.| Biblioteca(s): Embrapa Algodão. |
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| 124. | | RADL, V.; ARAUJO, J. L. S. de; LEITE, J.; PASSOS, S. R.; MARTINS, L. M. V.; XAVIER, G. R.; RUMJANEK, N. G.; BALDANI, J. I.; ZILLI, J. E. Microvirga vignae sp. nov., a root nodule symbiotic bacterium isolated from cowpea grown in semi-arid of Brazil. International Journal os Systematic and Evolutionary Microbiology, 31 oct. 2013.| Biblioteca(s): Embrapa Agrobiologia. |
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| 125. | | CARVALHO, P. de A.; MENESES, C. H. S. G.; ROUWS, L. F. M.; ARAUJO, J. L. S. de; VIDAL, M. S.; BALDANI, J. I. Mutante de uma provável endoglicanase de Gluconacetobacter diazotrophicus PAL5 é defectivo na adesão em raízes de arroz. In:SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 10., 18 a 24 de outubro de 2010, Seropédica. Ciência para o Desenvolvimento Sustentável: anais... Seropédica: Embrapa Agrobiologia, 2010.| Biblioteca(s): Embrapa Agrobiologia. |
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| 126. | | VIDEIRA, S. S.; GALVAO, P. G.; MORAIS, R. F. de; BALDANI, J. I.; ARAÚJO, J. L. S. de; URQUIAGA, S.; BALDANI, V. L. D. Ocorrência de bactérias diazotróficas associadas a dois cultivares de capim-elefante. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 32.; 2 a 7 da agosto de 2009, Fortaleza. O solo e a produção de bionergia: perspectivas e desafios: trabalhos científicos.... Fortaleza: Sociedade Brasileira de Ciência do Solo, 2009.| Biblioteca(s): Embrapa Agrobiologia. |
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| 127. | | OSEI, O.; ARAUJO, J. L. S. de; ZILLI, J. E.; BODDEY, R. M.; AHIABOR, B. D. K.; ABAIDOO, R. C.; ROUWS, L. F. M. PCR assay for direct specific detection of Bradyrhizobium elite strain BR 3262 in root nodule extracts of soil-grown cowpea Plant and Soil, v. 417, n. 1–2, p. 535–548, 2017| Biblioteca(s): Embrapa Agrobiologia. |
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| 132. | | SILA, H. A. P. da; GALISA, P. de S.; SILVA, J. R.; GONÇALO, T. P.; VIDAL, M. S.; ARAUJO, J. L. S. de. Prospecção de genes diferencialmente expressos em nódulos de feijão-caupi submetido a estresse térmico e déficit hídrico. In: SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 10., 18 a 24 de outubro de 2010, Seropédica. Ciência para o Desenvolvimento Sustentável: anais... Seropédica: Embrapa Agrobiologia, 2010.| Biblioteca(s): Embrapa Agrobiologia. |
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| 134. | | SOARES, C. de P.; RODRIGUES, E. P.; FERREIRA, J. P.; ARAUJO, J. L. S. de; ROUWS, L. F. M.; BALDANI, J. I.; VIDAL, M. S. Tn5 insertion in the tonB gene promoter affects iron-related phenotypes and increases extracellular siderophore levels in Gluconacetobacter diazotrophicus Archives of Microbiology Online, 17 oct. 2014. 11 p. A versão impressa foi publicada no v. 197, p. 223-233, 2015.| Biblioteca(s): Embrapa Agrobiologia. |
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| 135. | | POLESE, V.; SILVA, P. R. A.; PESSOA, D. D. V.; TADRA-SFEIR, M. Z.; SOUZA, E. M.; ARAUJO, J. L. S. de; BALDANI, J. I.; VIDAL, M. S. Transcriptional profile of Herbaspirillum rubrisubalbicans in response of sugarcane apoplastic fluid. In: WORLD CONGRESS ON GENETICS APPLIED TO LIVESTOCK PRODUCTION, 11., 2018, Auckland, New Zealand. Proceedings... Massey University, 2018.| Biblioteca(s): Embrapa Agrobiologia. |
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| 139. | | SILVA, P. R. A. da; VIDAL, M. S.; TADRA-SFEIR, M.Z.; ARAUJO, J. L. S. de; SOUZA, E. M. de; BALDANI, J. I. In vitro study suggests that stem apoplastic liquid of sugarcance modulates the chemotaxis and defense mechanism of the diazotrophic Burkholderia tropica strain PPe8 In: SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION WITH NON-LEGUMES, 15.; SYMPOSIUM OF THE EUROPEAN NITROGEN FIXATION CONFERENCE, 12., 2016, Hungary. Book of abstracts. Hungary: Biological Research Center, 2016. p. 47| Biblioteca(s): Embrapa Agrobiologia. |
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| 140. | | LEITE, J.; PASSOS, S. R.; ARAUJO, J. L. S. de; RUMJANEK, N. G.; XAVIER, G. R.; ZILLI, J. E.; RADL, V. What we know about the novel legume-nodulating Microvirga vignae? In: REUNIÃO LATINOAMERICANA DE RIZOBIOLOGIA, 27., 2016, Londrina. Fortalecendo as parcerias Sul-Sul: anais. Curitiba: SBCS-NEPAR, 2016. 308 p. Editado por Mariangle Hungria, Douglas Fabiano Gomes, Arnaldo Colozzi Filho. RELAR 2016. p. 169| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 168 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
24/11/2016 |
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Data da última atualização: |
23/10/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SILVA, P. R. A. da; VIDAL, M. S.; SOARES, C. de P.; POLESE, V.; ARAUJO, J. L. S. de; BALDANI, J. I. |
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Afiliação: |
PAULA RENATA ALVES DA SILVA, UFRRJ; MARCIA SOARES VIDAL, CNPAB; CLEITON DE PAULA SOARES, BOLSISTA DA EMBRAPA AGROBIOLOGIA; VALERIA POLESE, UFRRJ; JEAN LUIZ SIMOES DE ARAUJO, CNPAB; JOSE IVO BALDANI, CNPAB. |
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Título: |
Selection and evaluation of reference genes for RT-qPCR expression studies on Burkholderia tropica strain Ppe8, a sugarcane-associated diazotrophic bacterium grown with different carbon sources or sugarcane juice |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Antonie van Leeuwenhoek, v. 109, n. 111, p. 1493-1502, 2016. |
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DOI: |
10.1007/s10482-016-0751-0 |
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Idioma: |
Inglês |
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Notas: |
Epub, 17 Aug., 2016. |
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Conteúdo: |
Among the members of the genus Burkholderia, Burkholderia tropica has the ability to fix nitrogen and promote sugarcane plant growth as well as act as a biological control agent. There is little information about how this bacterium metabolizes
carbohydrates as well as those carbon sources found in the sugarcane juice that accumulates in stems during plant growth. Reverse transcription quantitative PCR (RT-qPCR) can be used to evaluate changes in gene expression during bacterial growth on different carbon sources. Here we tested the expression of six reference genes, lpxC, gyrB, recA, rpoA, rpoB, and rpoD, when cells were grown with glucose, fructose, sucrose, mannitol, aconitic acid, and sugarcane juice as carbon sources. The lpxC, gyrB, and recA were selected as the most stable reference genes based on geNorm and NormFinder software analyses. Validation of these three reference genes during strain Ppe8 growth on the same carbon sources showed that genes involved in
glycogen biosynthesis (glgA, glgB, glgC) and trehalose biosynthesis (treY and treZ) were highly expressed when Ppe8 was grown in aconitic acid relative to other carbon sources, while otsA expression (trehalose biosynthesis) was reduced with all carbon
sources. In addition, the expression level of the ORF_6066 (gluconolactonase) gene was reduced on sugarcane juice. The results confirmed the stability of the three selected reference genes (lpxC, gyrB, and recA) during the RT-qPCR and also their robustness
by evaluating the relative expression of genes involved in glycogen and trehalose biosynthesis when strain Ppe8 was grown on different carbon sources and sugarcane juice. MenosAmong the members of the genus Burkholderia, Burkholderia tropica has the ability to fix nitrogen and promote sugarcane plant growth as well as act as a biological control agent. There is little information about how this bacterium metabolizes
carbohydrates as well as those carbon sources found in the sugarcane juice that accumulates in stems during plant growth. Reverse transcription quantitative PCR (RT-qPCR) can be used to evaluate changes in gene expression during bacterial growth on different carbon sources. Here we tested the expression of six reference genes, lpxC, gyrB, recA, rpoA, rpoB, and rpoD, when cells were grown with glucose, fructose, sucrose, mannitol, aconitic acid, and sugarcane juice as carbon sources. The lpxC, gyrB, and recA were selected as the most stable reference genes based on geNorm and NormFinder software analyses. Validation of these three reference genes during strain Ppe8 growth on the same carbon sources showed that genes involved in
glycogen biosynthesis (glgA, glgB, glgC) and trehalose biosynthesis (treY and treZ) were highly expressed when Ppe8 was grown in aconitic acid relative to other carbon sources, while otsA expression (trehalose biosynthesis) was reduced with all carbon
sources. In addition, the expression level of the ORF_6066 (gluconolactonase) gene was reduced on sugarcane juice. The results confirmed the stability of the three selected reference genes (lpxC, gyrB, and recA) during the RT-qPCR and also their robustness
by evalua... Mostrar Tudo |
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Palavras-Chave: |
Carbon metabolism; Diazotrophic bacteria; Normalizing genes; Real time PCR; Relative expression. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
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001 2057134
005 2018-10-23
008 2016 bl --- 0-- u #d
024 7 $a10.1007/s10482-016-0751-0$2DOI
100 1 $aSILVA, P. R. A. da
245 $aSelection and evaluation of reference genes for RT-qPCR expression studies on Burkholderia tropica strain Ppe8, a sugarcane-associated diazotrophic bacterium grown with different carbon sources or sugarcane juice
260 $c2016
500 $aEpub, 17 Aug., 2016.
520 $aAmong the members of the genus Burkholderia, Burkholderia tropica has the ability to fix nitrogen and promote sugarcane plant growth as well as act as a biological control agent. There is little information about how this bacterium metabolizes carbohydrates as well as those carbon sources found in the sugarcane juice that accumulates in stems during plant growth. Reverse transcription quantitative PCR (RT-qPCR) can be used to evaluate changes in gene expression during bacterial growth on different carbon sources. Here we tested the expression of six reference genes, lpxC, gyrB, recA, rpoA, rpoB, and rpoD, when cells were grown with glucose, fructose, sucrose, mannitol, aconitic acid, and sugarcane juice as carbon sources. The lpxC, gyrB, and recA were selected as the most stable reference genes based on geNorm and NormFinder software analyses. Validation of these three reference genes during strain Ppe8 growth on the same carbon sources showed that genes involved in glycogen biosynthesis (glgA, glgB, glgC) and trehalose biosynthesis (treY and treZ) were highly expressed when Ppe8 was grown in aconitic acid relative to other carbon sources, while otsA expression (trehalose biosynthesis) was reduced with all carbon sources. In addition, the expression level of the ORF_6066 (gluconolactonase) gene was reduced on sugarcane juice. The results confirmed the stability of the three selected reference genes (lpxC, gyrB, and recA) during the RT-qPCR and also their robustness by evaluating the relative expression of genes involved in glycogen and trehalose biosynthesis when strain Ppe8 was grown on different carbon sources and sugarcane juice.
653 $aCarbon metabolism
653 $aDiazotrophic bacteria
653 $aNormalizing genes
653 $aReal time PCR
653 $aRelative expression
700 1 $aVIDAL, M. S.
700 1 $aSOARES, C. de P.
700 1 $aPOLESE, V.
700 1 $aARAUJO, J. L. S. de
700 1 $aBALDANI, J. I.
773 $tAntonie van Leeuwenhoek$gv. 109, n. 111, p. 1493-1502, 2016.
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