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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
06/12/2010 |
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Data da última atualização: |
09/02/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ABREU, E. F. M.; JESUS, C. de J.; ANDRADE, E. C. de; ARAGAO, F. J. L.; VIANNA, G. R. |
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Afiliação: |
EMANUEL FELIPE MEDEIROS ABREU, CNPMF; CAMILA C. de JESUS, UFRB; EDUARDO CHUMBINHO DE ANDRADE, CNPMF; FRANCISCO JOSE LIMA ARAGAO, CENARGEN; GIOVANNI RODRIGUES VIANNA, CENARGEN. |
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Título: |
Genetic transformation of Carica papaya to obtaining tolerance to abiotc stress. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Anais... Fortaleza: Sociedade Brasileira de Biotecnologia. |
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Páginas: |
338 p. |
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Idioma: |
Inglês |
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Conteúdo: |
Due to a new climatic reality that we have together , which causes scarcity of water resources, increased salinity and constant temperature change, increasingly we to obtain plants able to adapt to abiotic stresses, which have a great impact on their growth and productivity. To minimize the adverse effects caused by theses factors, the plant starts production of molecules involved in response and is essential for their survival, as HSPs (heat shock proteins). These molecules are responsible for protein folding, assembly, translocation and degradation in a broad array of normal cellular process; they also function in the stabilization of proteins and membranes, and can assist in protein refolding under stress conditions (Wang et al., 2004). The paper aims is generate transgenic C. papaya expressing sHsps obtained from Musa acuminata leaves subjected to heat and e cold, and evaluate the correlation betwen the response to abiotic stresses with the differential expression of these proteins. For genetic transformation, green fruit open pollinated "Sunrise" papaya, harvested 90 to 120 days after fruit set, was obtained, from germplasm bank from Embrapa Cassava and Tropical Fruit. Immature zygotic embryos were surface-sterilized with 70% ethanol followed by immersion in 1% sodium hypochlorite for 20 min and then rinsed three times in sterile water. The embryos were placed on induction medium that was the same as that reported by Fitch et al. (1990). For each bombardment test higly embryogenic cultures were selected and about 13-15 somatic embryo clumps were spread onto a filter paper. Thus three vectores (pUCBAr 194, pUCBar163 and pUCBar129) were constructed. Each vector constructed one different sHsps genes, the Bar gene selection gene and sGFP gene, all under control of the CaMV 35s promoter. After each bombarded the embryos were then transferred onto intuction medium (Ficth et al. 1990) incubated for 2-3 weeks in the dark at 28. C. To present an initial analysis performed 7 days after bombardment; a small tissue sample from each plate was tested for transient sGFP expression. Observatiion under a dissecting microscope revealed numerous spots in simples from DNA-bombarded plates. MenosDue to a new climatic reality that we have together , which causes scarcity of water resources, increased salinity and constant temperature change, increasingly we to obtain plants able to adapt to abiotic stresses, which have a great impact on their growth and productivity. To minimize the adverse effects caused by theses factors, the plant starts production of molecules involved in response and is essential for their survival, as HSPs (heat shock proteins). These molecules are responsible for protein folding, assembly, translocation and degradation in a broad array of normal cellular process; they also function in the stabilization of proteins and membranes, and can assist in protein refolding under stress conditions (Wang et al., 2004). The paper aims is generate transgenic C. papaya expressing sHsps obtained from Musa acuminata leaves subjected to heat and e cold, and evaluate the correlation betwen the response to abiotic stresses with the differential expression of these proteins. For genetic transformation, green fruit open pollinated "Sunrise" papaya, harvested 90 to 120 days after fruit set, was obtained, from germplasm bank from Embrapa Cassava and Tropical Fruit. Immature zygotic embryos were surface-sterilized with 70% ethanol followed by immersion in 1% sodium hypochlorite for 20 min and then rinsed three times in sterile water. The embryos were placed on induction medium that was the same as that reported by Fitch et al. (1990). For each bombardment test h... Mostrar Tudo |
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Palavras-Chave: |
Papaya; Transformação genética. |
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Thesagro: |
Mamão. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02889naa a2200217 a 4500
001 1868781
005 2011-02-09
008 2010 bl uuuu u00u1 u #d
100 1 $aABREU, E. F. M.
245 $aGenetic transformation of Carica papaya to obtaining tolerance to abiotc stress.
260 $c2010
300 $a338 p.
520 $aDue to a new climatic reality that we have together , which causes scarcity of water resources, increased salinity and constant temperature change, increasingly we to obtain plants able to adapt to abiotic stresses, which have a great impact on their growth and productivity. To minimize the adverse effects caused by theses factors, the plant starts production of molecules involved in response and is essential for their survival, as HSPs (heat shock proteins). These molecules are responsible for protein folding, assembly, translocation and degradation in a broad array of normal cellular process; they also function in the stabilization of proteins and membranes, and can assist in protein refolding under stress conditions (Wang et al., 2004). The paper aims is generate transgenic C. papaya expressing sHsps obtained from Musa acuminata leaves subjected to heat and e cold, and evaluate the correlation betwen the response to abiotic stresses with the differential expression of these proteins. For genetic transformation, green fruit open pollinated "Sunrise" papaya, harvested 90 to 120 days after fruit set, was obtained, from germplasm bank from Embrapa Cassava and Tropical Fruit. Immature zygotic embryos were surface-sterilized with 70% ethanol followed by immersion in 1% sodium hypochlorite for 20 min and then rinsed three times in sterile water. The embryos were placed on induction medium that was the same as that reported by Fitch et al. (1990). For each bombardment test higly embryogenic cultures were selected and about 13-15 somatic embryo clumps were spread onto a filter paper. Thus three vectores (pUCBAr 194, pUCBar163 and pUCBar129) were constructed. Each vector constructed one different sHsps genes, the Bar gene selection gene and sGFP gene, all under control of the CaMV 35s promoter. After each bombarded the embryos were then transferred onto intuction medium (Ficth et al. 1990) incubated for 2-3 weeks in the dark at 28. C. To present an initial analysis performed 7 days after bombardment; a small tissue sample from each plate was tested for transient sGFP expression. Observatiion under a dissecting microscope revealed numerous spots in simples from DNA-bombarded plates.
650 $aMamão
653 $aPapaya
653 $aTransformação genética
700 1 $aJESUS, C. de J.
700 1 $aANDRADE, E. C. de
700 1 $aARAGAO, F. J. L.
700 1 $aVIANNA, G. R.
773 $tIn: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Anais... Fortaleza: Sociedade Brasileira de Biotecnologia.
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