| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Café. |
|
Data corrente: |
13/04/2011 |
|
Data da última atualização: |
13/04/2011 |
|
Tipo da produção científica: |
Artigo em Anais de Congresso |
|
Autoria: |
FRANCO, O. L.; ANDRADE, A. E.; BARROS, E. V. S. A.; SA, M. F. G. de; CARNEIRO, R. M. D. G.; CARNEIRO, R.; EIRA, M. T. S. da; ROCHA, T. L.; REIS, A. M. dos. |
|
Afiliação: |
UNIVERSIDADE CATÓLICA DE BRASÍLIA; MARIA FATIMA GROSSI DE SA, CENARGEN; REGINA MARIA DECHECHI G CARNEIRO, CENARGEN; IAPAR; MIRIAN THEREZINHA SOUZA DA EIRA, SAPC; THALES LIMA ROCHA, CENARGEN; ANGELA MEHTA DOS REIS, CENARGEN. |
|
Título: |
Comparison of different staining methods for coffee proteomic analysis. |
|
Ano de publicação: |
2007 |
|
Fonte/Imprenta: |
In:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2007. 1 CD-ROM. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Proteomic methods, such as bidimensional electrophoresis (2-DE) and mass spectrometry, have been extensively used for the study of protein differential expression in several plants including Arabidopsis thaliana, rice and wheat. Specifically in the 2-DE method, deep attention must be given to the protein staining technique, which often involves silver nitrate or Coomassie Brilliant Blue (CBB). Silver staining is usually preferred over CBB due to the higher sensitivity obtained. Nevertheless, silver-staining resolution could significantly vary according to the studied organism and more specifically to the researched tissue. In Coffea spp., 2-DE analysis has been rarely employed. Some studies of protein expression have been reported in this culture mainly involving the biosynthesis of caffeine and metabolism during seed germination. The study of the global protein expression in coffee plants in response to biotic stress conditions had not been reported until now. Phytonematode infection can be considered one of the most important biotic stresses that affect coffee production and Meloidogyne paranaensis is one of the major nematode species that infects coffee plants. In this report, the protein expression of infected- and non-infected roots of coffee (Coffea canephora) were analyzed and the protein pattern determined by 2-DE. Gels were stained with silver nitrate or CBB, in order to obtain an optimized method for proteomic analysis of plant-nematode interaction. The 2-DE analysis revealed an enhanced number of protein spots, as well as differentially expressed proteins, when CBB was used. A total of approximately 70 and 100 spots were observed in silver and CBB stained gels, respectively. Moreover, 18 differentially expressed proteins were observed in CBB gels, and only 8 in the silver stained gels. This report showed that the staining method was crucial for an optimized protein analysis of coffee. Similar results were obtained for cotton roots and therefore these results may be extended to other plant species in order to better understand the host-pathogen interaction. MenosProteomic methods, such as bidimensional electrophoresis (2-DE) and mass spectrometry, have been extensively used for the study of protein differential expression in several plants including Arabidopsis thaliana, rice and wheat. Specifically in the 2-DE method, deep attention must be given to the protein staining technique, which often involves silver nitrate or Coomassie Brilliant Blue (CBB). Silver staining is usually preferred over CBB due to the higher sensitivity obtained. Nevertheless, silver-staining resolution could significantly vary according to the studied organism and more specifically to the researched tissue. In Coffea spp., 2-DE analysis has been rarely employed. Some studies of protein expression have been reported in this culture mainly involving the biosynthesis of caffeine and metabolism during seed germination. The study of the global protein expression in coffee plants in response to biotic stress conditions had not been reported until now. Phytonematode infection can be considered one of the most important biotic stresses that affect coffee production and Meloidogyne paranaensis is one of the major nematode species that infects coffee plants. In this report, the protein expression of infected- and non-infected roots of coffee (Coffea canephora) were analyzed and the protein pattern determined by 2-DE. Gels were stained with silver nitrate or CBB, in order to obtain an optimized method for proteomic analysis of plant-nematode interaction. The 2-DE analys... Mostrar Tudo |
|
Palavras-Chave: |
Bidimensional electrophoresis; Proteomic method. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32592/1/Comparison-of-Different.pdf
|
|
Marc: |
LEADER 02933nam a2200229 a 4500
001 1885752
005 2011-04-13
008 2007 bl uuuu u00u1 u #d
100 1 $aFRANCO, O. L.
245 $aComparison of different staining methods for coffee proteomic analysis.$h[electronic resource]
260 $aIn:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2007. 1 CD-ROM.$c2007
520 $aProteomic methods, such as bidimensional electrophoresis (2-DE) and mass spectrometry, have been extensively used for the study of protein differential expression in several plants including Arabidopsis thaliana, rice and wheat. Specifically in the 2-DE method, deep attention must be given to the protein staining technique, which often involves silver nitrate or Coomassie Brilliant Blue (CBB). Silver staining is usually preferred over CBB due to the higher sensitivity obtained. Nevertheless, silver-staining resolution could significantly vary according to the studied organism and more specifically to the researched tissue. In Coffea spp., 2-DE analysis has been rarely employed. Some studies of protein expression have been reported in this culture mainly involving the biosynthesis of caffeine and metabolism during seed germination. The study of the global protein expression in coffee plants in response to biotic stress conditions had not been reported until now. Phytonematode infection can be considered one of the most important biotic stresses that affect coffee production and Meloidogyne paranaensis is one of the major nematode species that infects coffee plants. In this report, the protein expression of infected- and non-infected roots of coffee (Coffea canephora) were analyzed and the protein pattern determined by 2-DE. Gels were stained with silver nitrate or CBB, in order to obtain an optimized method for proteomic analysis of plant-nematode interaction. The 2-DE analysis revealed an enhanced number of protein spots, as well as differentially expressed proteins, when CBB was used. A total of approximately 70 and 100 spots were observed in silver and CBB stained gels, respectively. Moreover, 18 differentially expressed proteins were observed in CBB gels, and only 8 in the silver stained gels. This report showed that the staining method was crucial for an optimized protein analysis of coffee. Similar results were obtained for cotton roots and therefore these results may be extended to other plant species in order to better understand the host-pathogen interaction.
653 $aBidimensional electrophoresis
653 $aProteomic method
700 1 $aANDRADE, A. E.
700 1 $aBARROS, E. V. S. A.
700 1 $aSA, M. F. G. de
700 1 $aCARNEIRO, R. M. D. G.
700 1 $aCARNEIRO, R.
700 1 $aEIRA, M. T. S. da
700 1 $aROCHA, T. L.
700 1 $aREIS, A. M. dos
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Café (CNPCa) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Hortaliças. |
|
Data corrente: |
07/12/2001 |
|
Data da última atualização: |
07/12/2001 |
|
Autoria: |
VIEIRA, J. V.; CHARCHAR, J. M.; BOITEUX, L. S.; ARAGÃO, F. A. S. |
|
Afiliação: |
EMBRAPA-CNPH, Brasília, DF. |
|
Título: |
Herdabilidade e ganho de seleção para resistência múltipla de campo aos nematóides Meloidogyne incógnita raça 1 e M. javanica em cenoura. |
|
Ano de publicação: |
2001 |
|
Fonte/Imprenta: |
Horticultura Brasileira, Brasília, DF, v. 19, n. 2, jul. 2001. |
|
Páginas: |
não paginado. |
|
Descrição Física: |
1 CD-ROM. |
|
Idioma: |
Português |
|
Notas: |
Trabalho apresentado no 41° Congresso Brasileiro de Olericultura, 2001. |
|
Palavras-Chave: |
Brasil; Brasília; Distrito Federal; Herdabilidade; Resistência múltipla. |
|
Thesagro: |
Cenoura; Cerrado; Daucus Carota; Doença; Meloidogyne Incognita; Meloidogyne Javanica; Nematóide. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 00998naa a2200313 a 4500
001 1770942
005 2001-12-07
008 2001 bl uuuu u00u1 u #d
100 1 $aVIEIRA, J. V.
245 $aHerdabilidade e ganho de seleção para resistência múltipla de campo aos nematóides Meloidogyne incógnita raça 1 e M. javanica em cenoura.
260 $c2001
300 $anão paginado. $c1 CD-ROM.
500 $aTrabalho apresentado no 41° Congresso Brasileiro de Olericultura, 2001.
650 $aCenoura
650 $aCerrado
650 $aDaucus Carota
650 $aDoença
650 $aMeloidogyne Incognita
650 $aMeloidogyne Javanica
650 $aNematóide
653 $aBrasil
653 $aBrasília
653 $aDistrito Federal
653 $aHerdabilidade
653 $aResistência múltipla
700 1 $aCHARCHAR, J. M.
700 1 $aBOITEUX, L. S.
700 1 $aARAGÃO, F. A. S.
773 $tHorticultura Brasileira, Brasília, DF$gv. 19, n. 2, jul. 2001.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Hortaliças (CNPH) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
