Data/hora: 05/06/2024 13:10:45

Biblioteca(s): | Embrapa Meio Ambiente.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | B - 1
Data corrente: | 17/11/2022
Data da última atualização: | 14/07/2023
Autoria: | ZWAR, I. P.; TROTTA, C. do V.; ZIOTTI, A. B. S.; LIMA NETO, M.; ARAÚJO, W. L. de; MELO, I. S. de; OTTONI, C. A.; SOUZA, A. O. de.
Afiliação: | INGRID PADOVESE ZWAR, INSTITUTO BUTANTAN; CATERINA DO VALLE TROTTA, UNIVERSIDADE ESTADUAL PAULISTA; ANA BEATRIZ SICCHIERI ZIOTTI, UNIVERSIDADE ESTADUAL PAULISTA; MILTON LIMA NETO, UNIVERSIDADE ESTADUAL PAULISTA; WELINGTON LUIZ DE ARAÚJO, UNIVERSIDADE DE SÃO PAULO; ITAMAR SOARES DE MELO, CNPMA; CRISTIANE ANGÉLICA OTTONI, UNIVERSIDADE ESTADUAL PAULISTA; ANA OLÍVIA DE SOUZA, INSTITUTO BUTANTAN.
Título: | Biosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogens.
Ano de publicação: | 2022
Fonte/Imprenta: | Journal of Basic Microbiology, v. 63, n. 1, p. 64-74, 2022.
ISSN: | 0233-111X
DOI: | https://doi.org/10.1002/jobm.202200439
Idioma: | Inglês
Conteúdo: | Abstract: To find effective silver nanoparticles (AgNPs) for control of phytopathogens, in this study, two strains of actinomycetes isolated from the soil of the Brazilian biome Caatinga (Caat5-35) and from mangrove sediment (Canv1-58) were utilized. The strains were identified by using the 16S rRNA gene sequencing as Streptomyces sp., related to Streptomyces mimosus species. The obtained AgNPs were coded as AgNPs 35 and AgNPs58 and characterized by size and morphology using dynamic light scattering, zeta potential, transmission electron microscopy, and Fourier transformed infrared (FTIR). The antifungal activity of the AgNPs35 and AgNPs58 was evaluated in vitro by the minimal inhibitory concentration (MIC) assay on the phytopathogens, Alternaria solani, Alternaria alternata, and Colletotrichum gloeosporioides. The phytotoxic effect was evaluated by the germination rate and seedling growth of rice (Oryza sativa). AgNPs35 and AgNPs58 showed surface plasmon resonance and average sizes of 30 and 60 nm, respectively. Both AgNPs presented spherical shape and the FTIR analysis confirmed the presence of functional groups such as free amines and hydroxyls of biomolecules bounded to the external layer of the nanoparticles. Both AgNPs inhibited the growth of the three phytopathogens tested, and A. alternate was the most sensible (MIC < = 4 µM). Moreover, the AgNPs35 and AgNPs58 did not induce phytotoxic effects on the germination and development of rice seedlings. In conclusion, these AgNPs are promising candidates to biocontrol of these phytopathogens without endangering rice plants.
Thesagro: | Actinomiceto; Controle Biológico; Doença de Planta.
Thesaurus Nal: | Actinomyces; Biological control agents; Fungal diseases of plants; Nanoparticles; Nanosilver.
Categoria do assunto: | H Saúde e Patologia
Registro original: | Embrapa Meio Ambiente (CNPMA)
Exemplares: | CNPMA - (17250-1) - UPC - AP - DD -  -  -  - DIS