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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
27/05/2016 |
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Data da última atualização: |
29/01/2018 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
TULLIO, L. D.; PAULITSCH, F.; REICHERT, P. R. S.; KLEPA, M. S.; KLEPA, M. S.; PILEGGI, M.; GOMES, D. F.; HUNGRIA, M.; BATISTA, J. S. S. |
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Afiliação: |
UEPG; UEPG; UEPG; UEPG; UEPG; MARIANGELA HUNGRIA DA CUNHA, CNPSO; UEPG. |
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Título: |
Proteomic analysis of Rhizobium freirei PRF 81 responses to low pH. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28.; SIMPÓSIO DE FERMENTAÇÃO ALCOÓLICA, 3.; SIMPÓSIO DE MICRORGANISMOS FOTOSSINTETIZANTES, 3.; SIMPÓSIO DE ESCHERICHIA COLI LUIZ RACHID TRABULSI, 4., 2015, Florianópolis. Anais... [São Paulo]: Sociedade Brasileira de Microbiologia, 2015. |
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Idioma: |
Português |
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Notas: |
Res. 1407-2. |
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Conteúdo: |
Rhizobium freirei PRF 81 is employed in common bean commercial inoculants in Brazil, due to its outstanding efficiency in fixing nitrogen, competitiveness and tolerance to abiotic stresses. Among the environmental conditions faced by rhizobia in soils, acidity is perhaps the encountered most, especially in Brazil. So, we used proteomics based approaches to study the responses of PRF 81 to a low pH condition. R. freirei PRF 81 was grown in TY medium until exponential phase in two treatments: pH 6,8 and pH 4,8. Whole-cell proteins were extracted and separated by two-dimensional gel electrophoresis, using IPG-strips with pH range 4-7 and 12% polyacrilamide gels. The experiment was performed in triplicate. Protein spots were detected in the high-resolution digitized gel images and analyzed by Image Master 2D Platinum v 5.0 software. Relative volumes (%vol) of compared between the two conditions tested and were statistically evaluated (p ≤ 0.05). Even knowing that R. freirei PRF 81 can still grow in more acid conditions, pH 4.8 was chosen because didn´t affect significantly the bacterial growth kinetics, a factor that could compromise the analysis. Using a narrow pH range, the gel profiles displayed a better resolution and reprodutibility than using broader pH range. Spots were mostly concentrated between pH 5-7 and molecular masses between 17-95 kDa. From the six hundred well-defined spots analyzed, one hundred and sixty-three spots presented a significant change in % vol, indicating that the pH led to expressive changes in the proteome of R. freirei PRF 81. Of these, sixty-one were up-regulated and one hundred two was downregulated in pH 4.8 condition. Also, fourteen spots were only identified in the acid condition, while seven spots was exclusively detected in pH 6.8. Ninety-five differentially expressed spots and two exclusively detected in pH 4,8 were selected for Maldi-Tof identification. Together with the genome sequencing and the proteome analysis of heat stress, we will search for molecular determinants of PRF 81 related to capacity to adapt to stressful tropical conditions. MenosRhizobium freirei PRF 81 is employed in common bean commercial inoculants in Brazil, due to its outstanding efficiency in fixing nitrogen, competitiveness and tolerance to abiotic stresses. Among the environmental conditions faced by rhizobia in soils, acidity is perhaps the encountered most, especially in Brazil. So, we used proteomics based approaches to study the responses of PRF 81 to a low pH condition. R. freirei PRF 81 was grown in TY medium until exponential phase in two treatments: pH 6,8 and pH 4,8. Whole-cell proteins were extracted and separated by two-dimensional gel electrophoresis, using IPG-strips with pH range 4-7 and 12% polyacrilamide gels. The experiment was performed in triplicate. Protein spots were detected in the high-resolution digitized gel images and analyzed by Image Master 2D Platinum v 5.0 software. Relative volumes (%vol) of compared between the two conditions tested and were statistically evaluated (p ≤ 0.05). Even knowing that R. freirei PRF 81 can still grow in more acid conditions, pH 4.8 was chosen because didn´t affect significantly the bacterial growth kinetics, a factor that could compromise the analysis. Using a narrow pH range, the gel profiles displayed a better resolution and reprodutibility than using broader pH range. Spots were mostly concentrated between pH 5-7 and molecular masses between 17-95 kDa. From the six hundred well-defined spots analyzed, one hundred and sixty-three spots presented a significant chan... Mostrar Tudo |
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Thesagro: |
Fixação de nitrogênio. |
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Thesaurus Nal: |
Nitrogen fixation. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/143444/1/R1407-2.PDF
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Marc: |
LEADER 03060nam a2200241 a 4500
001 2045758
005 2018-01-29
008 2015 bl uuuu u00u1 u #d
100 1 $aTULLIO, L. D.
245 $aProteomic analysis of Rhizobium freirei PRF 81 responses to low pH.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28.; SIMPÓSIO DE FERMENTAÇÃO ALCOÓLICA, 3.; SIMPÓSIO DE MICRORGANISMOS FOTOSSINTETIZANTES, 3.; SIMPÓSIO DE ESCHERICHIA COLI LUIZ RACHID TRABULSI, 4., 2015, Florianópolis. Anais... [São Paulo]: Sociedade Brasileira de Microbiologia$c2015
500 $aRes. 1407-2.
520 $aRhizobium freirei PRF 81 is employed in common bean commercial inoculants in Brazil, due to its outstanding efficiency in fixing nitrogen, competitiveness and tolerance to abiotic stresses. Among the environmental conditions faced by rhizobia in soils, acidity is perhaps the encountered most, especially in Brazil. So, we used proteomics based approaches to study the responses of PRF 81 to a low pH condition. R. freirei PRF 81 was grown in TY medium until exponential phase in two treatments: pH 6,8 and pH 4,8. Whole-cell proteins were extracted and separated by two-dimensional gel electrophoresis, using IPG-strips with pH range 4-7 and 12% polyacrilamide gels. The experiment was performed in triplicate. Protein spots were detected in the high-resolution digitized gel images and analyzed by Image Master 2D Platinum v 5.0 software. Relative volumes (%vol) of compared between the two conditions tested and were statistically evaluated (p ≤ 0.05). Even knowing that R. freirei PRF 81 can still grow in more acid conditions, pH 4.8 was chosen because didn´t affect significantly the bacterial growth kinetics, a factor that could compromise the analysis. Using a narrow pH range, the gel profiles displayed a better resolution and reprodutibility than using broader pH range. Spots were mostly concentrated between pH 5-7 and molecular masses between 17-95 kDa. From the six hundred well-defined spots analyzed, one hundred and sixty-three spots presented a significant change in % vol, indicating that the pH led to expressive changes in the proteome of R. freirei PRF 81. Of these, sixty-one were up-regulated and one hundred two was downregulated in pH 4.8 condition. Also, fourteen spots were only identified in the acid condition, while seven spots was exclusively detected in pH 6.8. Ninety-five differentially expressed spots and two exclusively detected in pH 4,8 were selected for Maldi-Tof identification. Together with the genome sequencing and the proteome analysis of heat stress, we will search for molecular determinants of PRF 81 related to capacity to adapt to stressful tropical conditions.
650 $aNitrogen fixation
650 $aFixação de nitrogênio
700 1 $aPAULITSCH, F.
700 1 $aREICHERT, P. R. S.
700 1 $aKLEPA, M. S.
700 1 $aKLEPA, M. S.
700 1 $aPILEGGI, M.
700 1 $aGOMES, D. F.
700 1 $aHUNGRIA, M.
700 1 $aBATISTA, J. S. S.
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Registro original: |
Embrapa Soja (CNPSO) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
08/09/2022 |
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Data da última atualização: |
12/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
ARAÚJO, C. de A.; MAGALHÃES, A. L. R.; ARAUJO, G. G. L. de; CAMPOS, F. S.; GOIS, G. C.; MATOS, M. H. T. de; QUEIROZ, M. A. A.; MENEZES, V. G.; COSTA, C. de J. P.; SANTOS, K. C. dos; LEITE, A. C. S. P. |
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Afiliação: |
CLEYTON DE ALMEIDA ARAÚJO, Universidade Federal do Agreste de Pernambuco - UFAPE; ANDRÉ LUIZ RODRIGUES MAGALHÃES, Universidade Federal do Agreste de Pernambuco - UFAPE; GHERMAN GARCIA LEAL DE ARAUJO, CPATSA; FLEMING SENA CAMPOS, Universidade Federal do Agreste de Pernambuco - UFAPE; GLAYCIANE COSTA GOIS, UNIVASF; MARIA HELENA TAVARES DE MATOS, UNIVASF; MÁRIO ADRIANO ÁVILA QUEIROZ, UNIVASF; VANÚZIA GONÇALVES MENEZES, Universidade Federal Rural de Pernambuco – UFRPE; CLAUDENILDE DE JESUS PINHEIRO COSTA, Universidade Federal do Agreste de Pernambuco - UFAPE; KELLY CRISTINA DOS SANTOS, Universidade Federal Rural de Pernambuco – UFRPE; ANA CLARA SILVA PINHEIRO LEITE, Universidade Federal do Agreste de Pernambuco - UFAPE. |
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Título: |
Effect of reduced of water supply on carcass characteristics, non-carcass components and the volume of digestive compartments of Santa Inês ewes. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Livestock Science, v. 245, 104402, 2021. |
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DOI: |
https://doi.org/10.1016/j.livsci.2021.104402 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to evaluate the effect of reducing water supply for watering of Santa In?es ewes on carcass characteristics, non-carcass components and the volume of digestive compartments. Thirty-two crossbred Santa In?es ewes, with an average body weight of 32.2 ± 7.4 kg, were distributed in a randomized block design with four treatments (different supply levels - 100% (ad libitum), 80%, 60% and 40% ad libitum water intake treatment) and eight repetitions. The animals were confined for 63 days until slaughter. The decrease in water availability promoted a linear decreasing effect for the internal carcass length (P = 0.032). A quadratic effect was found for the volume of the omasum (P = 0.018) and abomasum (P = 0.039), with maximum volumes of 0.305 and 1.636 liters, respectively, for 40% supply. The reticulum yield showed a quadratic effect (P = 0.044) with a maximum point in the supply of 100%, with 0.527%. The reduction in water supply promoted a quadratic effect for the weight (P = 0.016) and yield (P = 0.030) of the bladder, with maximum yields found in the supply of 60%, with 0.067 kg and 0.233%, respectively. There was a quadratic effect for weight (P = 0.042) and yield (P = 0.029) of the diaphragm with higher values found in the supply of 60%m with 0.272 kg and 0.927%, respectively. There was a quadratic effect for kidney yield (P = 0.022) with higher yield for 100% supply. Therefore, the reduction in water supply levels by up to 40% in relation to voluntary intake can be a strategy to be used in cases of water scarcity, as it does not cause negative effects on the Santa In?es ewe carcass. MenosThe aim of this study was to evaluate the effect of reducing water supply for watering of Santa In?es ewes on carcass characteristics, non-carcass components and the volume of digestive compartments. Thirty-two crossbred Santa In?es ewes, with an average body weight of 32.2 ± 7.4 kg, were distributed in a randomized block design with four treatments (different supply levels - 100% (ad libitum), 80%, 60% and 40% ad libitum water intake treatment) and eight repetitions. The animals were confined for 63 days until slaughter. The decrease in water availability promoted a linear decreasing effect for the internal carcass length (P = 0.032). A quadratic effect was found for the volume of the omasum (P = 0.018) and abomasum (P = 0.039), with maximum volumes of 0.305 and 1.636 liters, respectively, for 40% supply. The reticulum yield showed a quadratic effect (P = 0.044) with a maximum point in the supply of 100%, with 0.527%. The reduction in water supply promoted a quadratic effect for the weight (P = 0.016) and yield (P = 0.030) of the bladder, with maximum yields found in the supply of 60%, with 0.067 kg and 0.233%, respectively. There was a quadratic effect for weight (P = 0.042) and yield (P = 0.029) of the diaphragm with higher values found in the supply of 60%m with 0.272 kg and 0.927%, respectively. There was a quadratic effect for kidney yield (P = 0.022) with higher yield for 100% supply. Therefore, the reduction in water supply levels by up to 40% in relation to voluntar... Mostrar Tudo |
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Palavras-Chave: |
Escassez de água; Escassez hídrica; Ovelhas Santa Inês; Pequenos ruminantes; Região Semiárida; Rendimento de carcaça. |
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Thesagro: |
Carcaça; Nutrição Animal; Ovelha. |
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Thesaurus NAL: |
Carcass yield; Ewes; Small ruminants. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02852naa a2200397 a 4500
001 2146236
005 2023-01-12
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.livsci.2021.104402$2DOI
100 1 $aARAÚJO, C. de A.
245 $aEffect of reduced of water supply on carcass characteristics, non-carcass components and the volume of digestive compartments of Santa Inês ewes.$h[electronic resource]
260 $c2021
520 $aThe aim of this study was to evaluate the effect of reducing water supply for watering of Santa In?es ewes on carcass characteristics, non-carcass components and the volume of digestive compartments. Thirty-two crossbred Santa In?es ewes, with an average body weight of 32.2 ± 7.4 kg, were distributed in a randomized block design with four treatments (different supply levels - 100% (ad libitum), 80%, 60% and 40% ad libitum water intake treatment) and eight repetitions. The animals were confined for 63 days until slaughter. The decrease in water availability promoted a linear decreasing effect for the internal carcass length (P = 0.032). A quadratic effect was found for the volume of the omasum (P = 0.018) and abomasum (P = 0.039), with maximum volumes of 0.305 and 1.636 liters, respectively, for 40% supply. The reticulum yield showed a quadratic effect (P = 0.044) with a maximum point in the supply of 100%, with 0.527%. The reduction in water supply promoted a quadratic effect for the weight (P = 0.016) and yield (P = 0.030) of the bladder, with maximum yields found in the supply of 60%, with 0.067 kg and 0.233%, respectively. There was a quadratic effect for weight (P = 0.042) and yield (P = 0.029) of the diaphragm with higher values found in the supply of 60%m with 0.272 kg and 0.927%, respectively. There was a quadratic effect for kidney yield (P = 0.022) with higher yield for 100% supply. Therefore, the reduction in water supply levels by up to 40% in relation to voluntary intake can be a strategy to be used in cases of water scarcity, as it does not cause negative effects on the Santa In?es ewe carcass.
650 $aCarcass yield
650 $aEwes
650 $aSmall ruminants
650 $aCarcaça
650 $aNutrição Animal
650 $aOvelha
653 $aEscassez de água
653 $aEscassez hídrica
653 $aOvelhas Santa Inês
653 $aPequenos ruminantes
653 $aRegião Semiárida
653 $aRendimento de carcaça
700 1 $aMAGALHÃES, A. L. R.
700 1 $aARAUJO, G. G. L. de
700 1 $aCAMPOS, F. S.
700 1 $aGOIS, G. C.
700 1 $aMATOS, M. H. T. de
700 1 $aQUEIROZ, M. A. A.
700 1 $aMENEZES, V. G.
700 1 $aCOSTA, C. de J. P.
700 1 $aSANTOS, K. C. dos
700 1 $aLEITE, A. C. S. P.
773 $tLivestock Science$gv. 245, 104402, 2021.
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