Data/hora: 07/06/2024 08:02:00

Biblioteca(s): | Embrapa Caprinos e Ovinos; Embrapa Pecuária Sul.
Data corrente: | 10/02/2020
Data da última atualização: | 01/12/2020
Autoria: | ALVES, J. R. A.; FARIAS, A. E. M. de; ANJOS, D. M. dos; LIMA, A. M. C.; FACCIOLI-MARTINS, P. Y.; SOUZA, C. J. H. de; PINHEIRO, R. R.; ALVES, F. S. F.; AZEVEDO, S. S. de; ALVES, C. J.
Afiliação: | JOSÉ ROMERO ALEXANDRE ALVES; AREANO ETHÉRIO MOREIRA DE FARIAS; DENIZE MONTEIRO DOS ANJOS; ANA MILENA CÉZAR LIMA; PATRICIA YOSHIDA FACCIOLI-MARTINS, CNPC; CARLOS JOSÉ HOFF DE SOUZA; RAYMUNDO RIZALDO PINHEIRO, CNPC; FRANCISCO SELMO FERNANDES ALVES, CNPC; SÉRGIO SANTOS DE AZEVEDO; CLEBERT JOSÉ ALVES.
Título: | Seroepidemiological study of Caseous lymphadenitis in sheep from the Northeast region of Brazil using an indirect ELISA.
Ano de publicação: | 2020
Fonte/Imprenta: | Tropical Animal Health and Production, v. 52, n. 4, p. 1945-1952, Jul. 2020.
DOI: | 10.1007/s11250-020-02214-9
Idioma: | Inglês
Conteúdo: | Abstract: The objective of this study was to determine the frequency of seropositivity Corynebacterium pseudotuberculosis in sheep in five states of northeastern Brazil, using an indirect enzyme-linked immunosorbent assay (i-ELISA). Young and adult sheep of both sexes were used. Blood samples were collected from 2638 sheep from 223 herds across all states. For the i-ELISA, antigens produced from the strain of C. pseudotuberculosis BRM 029971, a bacterial isolate from the Northeast region of Brazil, were used. Sensitivity and specificity indexes were calculated for the validation of the test, using as reference 49 and 134 serum samples from sheep known to be positive and negative, respectively. The i-ELISA presented four false-negative and four false-positive results, showing a specificity of 97.01%, a sensitivity of 91.84%, and an accuracy of 95.63%. These results were calculated based on an optical density (OD) cutoff point?=?0.138. Of the 2638 sheep tested, 996 (37.76%, 95% CI?=?35.93-39.62%) were seropositive, and of the 223 evaluated herds, 210 (94.17%, 95% CI?=?90.28-96.56%) seropositive. The i-ELISA showed adequate sensitivity and specificity, proving to be a useful tool in the diagnosis of caseous lymphadenitis (CLA) in sheep. Infection by C. pseudotuberculosis, determined by serology, is disseminated in the sheep herds in the states of Northeast Brazil. Thus, there is a need to implement effective control measures that prevent the spread of infectious agents.
Palavras-Chave: | Brazilian northeast; Sorologia.
Thesagro: | Bactéria; Bacteriose; Corynebacterium Pseudotuberculosis; Doença Animal; Elisa; Linfadenite Caseosa; Ovino.
Thesaurus Nal: | Caseous lymphadenitis; Enzyme-linked immunosorbent assay; Epidemiological studies; Serology; Sheep diseases.
Categoria do assunto: | H Saúde e Patologia
Registro original: | Embrapa Caprinos e Ovinos (CNPC)

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