Data/hora: 16/06/2024 03:40:09

Biblioteca(s): | Embrapa Café.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | B - 4
Data corrente: | 27/03/2023
Data da última atualização: | 12/04/2023
Autoria: | ANGELO, P. C. da S.; PEREIRA, L. F. P.; SERA, G. H.; SETA, T.
Afiliação: | PAULA CRISTINA DA SILVA ANGELO, CNPCa; L. F. P. PEREIRA, IDR - PARANÁ; G. H. SERA, IDR - PARANÁ; T. SERA, IDR - PARANÁ.
Título: | Coffee microspore cultivation to attain doubled-haploid plantlets.
Ano de publicação: | 2023
Fonte/Imprenta: | Acta Horticulturae, v. 31, n. 1359, p. 123-130, 2023.
DOI: | 10.17660/ActaHortic.2023.1359.14
Idioma: | Inglês
Notas: | Apresentado no INTERNATIONAL HORTICULTURAL CONGRESS, 31., 2022, Angers, França.
Conteúdo: | Introgression of disease resistance from related Coffea species to C. arabica has been done by inter-specific hybridization. C. arabica (tetraploid) × C. racemosa (diploid) hybrids were obtained at the IDR-Paraná, Brazil. Some of the hybrids display impaired self-pollination, despite backcrossed to C. arabica. As an attempt to regenerate stable doubled-haploid plants, anthers and isolated microspores have been cultivated in vitro. It was considered that chromosomes captured in a microspore following meiosis could acquire homo and/or homeologous stability faster in vitro than in vivo. A C. arabica progeny was taken as control. Flowers were collected at the IDR campus in Londrina, when young microspores were uninucleate, and treated with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer set to function for a few seconds, washed and centrifuged at 100× g twice, and cultivated in modified N6 liquid medium (105 cells mL-1 in 35 mm diameter plates). Anthers were excised using scalpels and cultivated in solid medium. Explants were kept for six months of continuous cultivation on that induction medium containing 6.5 mg L-1 auxins, 1.0 mg L-1 cytokinins and 0.5 mg L-1 gibberellin (GA3), at 27°C under dark. Microspores produced embryo-like structures or microcalli in very low frequencies (0.3 per plate). Anthers, on the other hand, produced embryogenic calli. Colonization by an invariant morphotype of fungus took away 70% of Arabica coffee control anthers and 5% of the asseptic anthers produced friable calli, some embryogenic but arrested when compared with calli produced by the inter-specific hybrid anthers. Colonization by an invariant morphotype of bacteria took away 25% of hybrid anthers and 8% of produced embryogenic tissue, with globular embryos simultaneously multiplying and maturing upon transfer to N6 medium. Embryo conversion and photomorphogenesis under light on auxin/cytokinin ratio = 2 plus GA3 are going on, 1.2 year after inoculation.
Thesagro: | Embriogénese; Pólen; Rubiaceae.
Thesaurus Nal: | Embryogenesis; Pollen.
Categoria do assunto: | --
Registro original: | Embrapa Café (CNPCa)
Exemplares: | CNPCa - (1692-1) - UPC - AP - DD -  -  -  - DIS