Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
10/12/2021 |
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Data da última atualização: |
10/12/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
ARAÚJO, J. F.; ANDRIOLI, A.; PINHEIRO, R. R.; PEIXOTO, R. M.; SOUSA, A. L. M. de; AZEVEDO, D. A. A. de; LIMA, A. M. C.; NOBRE, J. A.; AMARAL, G. P.; BRANDÃO, I. S.; TEIXEIRA, M. F. da S. |
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Afiliação: |
JUSCILÂNIA FURTADO ARAÚJO, State University of Ceará - Fortaleza, Ceará, Brazil; ALICE ANDRIOLI PINHEIRO, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; RENATO MESQUITA PEIXOTO, Scholarship for Regional Scientific Development of the National Council for Scientific and Technological Development (DCR-CNPq/FUNCAP), Level C, Brasilia, Distrito Federal, Brazil; ANA LÍDIA MADEIRA DE SOUSA; DALVA ALANA ARAGÃO DE AZEVEDO, State University of Ceará - Fortaleza, Ceará, Brazil; ANA MILENA CESAR LIMA, Federal University of Piauí - Teresina, Piauí, Brazil; JULIANA ARAÚJO NOBRE, State University of Ceará - Fortaleza, Ceará, Brazil; GABRIEL PAULA AMARAL, State University of Acaraú Valley, Sobral, Ceará, Brazil; IANE SOUSA BRANDÃO, State University of Acaraú Valley, Sobral, Ceará, Brazil; MARIA FÁTIMA DA SILVA TEIXEIRA, State University of Ceará - Fortaleza, Ceará, Brazil. |
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Título: |
Detection and isolation of small ruminant lentivirus in the amniotic fluid of goats. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Comparative Immunology, Microbiology and Infectious Diseases, v. 78, 101693, Oct. 2021. |
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DOI: |
https://doi.org/10.1016/j.cimid.2021.101693 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract:The objective of this study was to verify the presence of small ruminant lentivirus in the amniotic fluid of goats using molecular tests and viral isolation by cocultivation in the amniotic fluid of naturally infected goats. The study analyzed eight goats: seven were small ruminant lentivirus-positive and one was negative. The amniotic fluid was collected from each of the eight animals during cesarean section at 147 days of pregnancy. Cocultivation was undertaken using secondary goat nictitating membrane cell cultures obtained by explant from a small ruminant lentivirus-negative calf followed by trypsinization and sub-cultivation of the cells for 63 days. During this period, five supernatant collections were performed for DNA extraction and subsequent nested polymerase chain reaction. DNA was extracted from the amniotic fluid after 3 h of cellular sedimentation, from which a sample of 600 ?L was taken from the sediment and another 600 ?L sample from the supernatant. After DNA extraction, nested polymerase chain reaction was performed. Of the eight goats, 62.5 % (05/08) were small ruminant lentivirus-positive, with 43.75 % (07/16) of the total samples positive when considering the two repetitions (supernatant and cell sediment). Moreover, positivity was confirmed by small ruminant lentivirus pro-viral DNA amplification in the cell supernatant throughout the cocultivation period. Small ruminant lentivirus were present in the amniotic fluid samples from the naturally infected goats indicating an intrauterine transmission route. Moreover, this biological fluid can be adopted for the diagnosis of these lentiviruse because it is an important risk factor related to intrauterine transmission. MenosAbstract:The objective of this study was to verify the presence of small ruminant lentivirus in the amniotic fluid of goats using molecular tests and viral isolation by cocultivation in the amniotic fluid of naturally infected goats. The study analyzed eight goats: seven were small ruminant lentivirus-positive and one was negative. The amniotic fluid was collected from each of the eight animals during cesarean section at 147 days of pregnancy. Cocultivation was undertaken using secondary goat nictitating membrane cell cultures obtained by explant from a small ruminant lentivirus-negative calf followed by trypsinization and sub-cultivation of the cells for 63 days. During this period, five supernatant collections were performed for DNA extraction and subsequent nested polymerase chain reaction. DNA was extracted from the amniotic fluid after 3 h of cellular sedimentation, from which a sample of 600 ?L was taken from the sediment and another 600 ?L sample from the supernatant. After DNA extraction, nested polymerase chain reaction was performed. Of the eight goats, 62.5 % (05/08) were small ruminant lentivirus-positive, with 43.75 % (07/16) of the total samples positive when considering the two repetitions (supernatant and cell sediment). Moreover, positivity was confirmed by small ruminant lentivirus pro-viral DNA amplification in the cell supernatant throughout the cocultivation period. Small ruminant lentivirus were present in the amniotic fluid samples from the naturally i... Mostrar Tudo |
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Palavras-Chave: |
Amniotic sac; Intrauterine transmission; Retrovirus; SRLVs; Vertical disease transmission. |
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Thesaurus NAL: |
Body fluids; Disease diagnosis; Goat diseases; Goats; Risk factors. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02839naa a2200373 a 4500
001 2137480
005 2021-12-10
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.cimid.2021.101693$2DOI
100 1 $aARAÚJO, J. F.
245 $aDetection and isolation of small ruminant lentivirus in the amniotic fluid of goats.$h[electronic resource]
260 $c2021
520 $aAbstract:The objective of this study was to verify the presence of small ruminant lentivirus in the amniotic fluid of goats using molecular tests and viral isolation by cocultivation in the amniotic fluid of naturally infected goats. The study analyzed eight goats: seven were small ruminant lentivirus-positive and one was negative. The amniotic fluid was collected from each of the eight animals during cesarean section at 147 days of pregnancy. Cocultivation was undertaken using secondary goat nictitating membrane cell cultures obtained by explant from a small ruminant lentivirus-negative calf followed by trypsinization and sub-cultivation of the cells for 63 days. During this period, five supernatant collections were performed for DNA extraction and subsequent nested polymerase chain reaction. DNA was extracted from the amniotic fluid after 3 h of cellular sedimentation, from which a sample of 600 ?L was taken from the sediment and another 600 ?L sample from the supernatant. After DNA extraction, nested polymerase chain reaction was performed. Of the eight goats, 62.5 % (05/08) were small ruminant lentivirus-positive, with 43.75 % (07/16) of the total samples positive when considering the two repetitions (supernatant and cell sediment). Moreover, positivity was confirmed by small ruminant lentivirus pro-viral DNA amplification in the cell supernatant throughout the cocultivation period. Small ruminant lentivirus were present in the amniotic fluid samples from the naturally infected goats indicating an intrauterine transmission route. Moreover, this biological fluid can be adopted for the diagnosis of these lentiviruse because it is an important risk factor related to intrauterine transmission.
650 $aBody fluids
650 $aDisease diagnosis
650 $aGoat diseases
650 $aGoats
650 $aRisk factors
653 $aAmniotic sac
653 $aIntrauterine transmission
653 $aRetrovirus
653 $aSRLVs
653 $aVertical disease transmission
700 1 $aANDRIOLI, A.
700 1 $aPINHEIRO, R. R.
700 1 $aPEIXOTO, R. M.
700 1 $aSOUSA, A. L. M. de
700 1 $aAZEVEDO, D. A. A. de
700 1 $aLIMA, A. M. C.
700 1 $aNOBRE, J. A.
700 1 $aAMARAL, G. P.
700 1 $aBRANDÃO, I. S.
700 1 $aTEIXEIRA, M. F. da S.
773 $tComparative Immunology, Microbiology and Infectious Diseases$gv. 78, 101693, Oct. 2021.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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