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 | Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
05/02/2024 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DUARTE, M. F.; ANDRADE, I. A. de; SILVA, J. M. F.; MELO, F. L. de; MACHADO, A. M.; INOUE-NAGATA, A. K.; NAGATA, T. |
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Afiliação: |
MACÁRIA FERREIRA DUARTE, UNIVERSIDADE DE BRASÍLIA; IKARO ALVES DE ANDRADE, UNIVERSIDADE DE BRASÍLIA; JOÃO MARCOS FAGUNDES SILVA, UNIVERSIDADE DE BRASÍLIA; FERNANDO LUCAS DE MELO, UNIVERSIDADE DE BRASILIA; ANA MARIA MACHADO, COMPANHIA DE SANEAMENTO AMBIENTAL DO DF; ALICE KAZUKO INOUE NAGATA, CNPH; TATSUYA NAGATA, UNIVERSIDADE DE BRASÍLIA. |
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Título: |
Metagenomic analyses of plant virus sequences in sewage water for plant viruses monitoring. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Tropical Plant Pathology, v. 48, p. 408-416, 2023. |
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DOI: |
10.1007/s40858-023-00575-8 |
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Idioma: |
Inglês |
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Conteúdo: |
Frequent monitoring of emerging viruses of agricultural crops is one of the most important missions for plant virologists. A fast and precise identifcation of potential harmful viruses may prevent the occurrence of serious epidemics. Nowadays, highthroughput sequencing (HTS) technologies became an accessible and powerful tool for this purpose. The major discussion of this strategy resides in the process of sample collection, which is usually laborious, costly and nonrepresentative. In this study, we assessed the use of sewage water samples for monitoring the widespread, numerous, and stable plant viruses using HTS analysis and RT-qPCR. Plant viruses belonged to 12 virus families were found, from which Virgaviridae, Solemoviridae, Tymoviridae, Alphafexiviridae, Betafexiviridae, losteroviridae and Secoviridae were the most abundant ones with more than 20 species. Additionally, we detected one quarantine virus in Brazil and a new tobamovirus species. To assess the importance of the processed foods as virus release origins to sewage, we selected two viruses, the tobamovirus pepper mild mottle virus (PMMoV) and the carlavirus garlic common latent virus (GarCLV), to detect in processed food materials by RT-qPCR. PMMoV was detected in large amount in pepper-based processed foods and in sewage samples, while GarCLV was less frequent in dried and fresh garlic samples, and in the sewage samples. This suggested a high correlation of virus abundance in sewage and processed food sources. The potential use of sewage for a virus survey is discussed in this study. MenosFrequent monitoring of emerging viruses of agricultural crops is one of the most important missions for plant virologists. A fast and precise identifcation of potential harmful viruses may prevent the occurrence of serious epidemics. Nowadays, highthroughput sequencing (HTS) technologies became an accessible and powerful tool for this purpose. The major discussion of this strategy resides in the process of sample collection, which is usually laborious, costly and nonrepresentative. In this study, we assessed the use of sewage water samples for monitoring the widespread, numerous, and stable plant viruses using HTS analysis and RT-qPCR. Plant viruses belonged to 12 virus families were found, from which Virgaviridae, Solemoviridae, Tymoviridae, Alphafexiviridae, Betafexiviridae, losteroviridae and Secoviridae were the most abundant ones with more than 20 species. Additionally, we detected one quarantine virus in Brazil and a new tobamovirus species. To assess the importance of the processed foods as virus release origins to sewage, we selected two viruses, the tobamovirus pepper mild mottle virus (PMMoV) and the carlavirus garlic common latent virus (GarCLV), to detect in processed food materials by RT-qPCR. PMMoV was detected in large amount in pepper-based processed foods and in sewage samples, while GarCLV was less frequent in dried and fresh garlic samples, and in the sewage samples. This suggested a high correlation of virus abundance in sewage and processed food sources.... Mostrar Tudo |
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Palavras-Chave: |
High-throughput sequencing. |
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Thesagro: |
Vírus. |
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Thesaurus Nal: |
Wastewater. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02294naa a2200241 a 4500
001 2161643
005 2024-02-05
008 2023 bl uuuu u00u1 u #d
024 7 $a10.1007/s40858-023-00575-8$2DOI
100 1 $aDUARTE, M. F.
245 $aMetagenomic analyses of plant virus sequences in sewage water for plant viruses monitoring.$h[electronic resource]
260 $c2023
520 $aFrequent monitoring of emerging viruses of agricultural crops is one of the most important missions for plant virologists. A fast and precise identifcation of potential harmful viruses may prevent the occurrence of serious epidemics. Nowadays, highthroughput sequencing (HTS) technologies became an accessible and powerful tool for this purpose. The major discussion of this strategy resides in the process of sample collection, which is usually laborious, costly and nonrepresentative. In this study, we assessed the use of sewage water samples for monitoring the widespread, numerous, and stable plant viruses using HTS analysis and RT-qPCR. Plant viruses belonged to 12 virus families were found, from which Virgaviridae, Solemoviridae, Tymoviridae, Alphafexiviridae, Betafexiviridae, losteroviridae and Secoviridae were the most abundant ones with more than 20 species. Additionally, we detected one quarantine virus in Brazil and a new tobamovirus species. To assess the importance of the processed foods as virus release origins to sewage, we selected two viruses, the tobamovirus pepper mild mottle virus (PMMoV) and the carlavirus garlic common latent virus (GarCLV), to detect in processed food materials by RT-qPCR. PMMoV was detected in large amount in pepper-based processed foods and in sewage samples, while GarCLV was less frequent in dried and fresh garlic samples, and in the sewage samples. This suggested a high correlation of virus abundance in sewage and processed food sources. The potential use of sewage for a virus survey is discussed in this study.
650 $aWastewater
650 $aVírus
653 $aHigh-throughput sequencing
700 1 $aANDRADE, I. A. de
700 1 $aSILVA, J. M. F.
700 1 $aMELO, F. L. de
700 1 $aMACHADO, A. M.
700 1 $aINOUE-NAGATA, A. K.
700 1 $aNAGATA, T.
773 $tTropical Plant Pathology$gv. 48, p. 408-416, 2023.
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Registro original: |
Embrapa Hortaliças (CNPH) |
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Registro |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria Tropical. Para informações adicionais entre em contato com cnpat.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria Tropical. |
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Data corrente: |
26/05/2021 |
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Data da última atualização: |
30/06/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 4 |
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Autoria: |
MENEZES, F. L. DE; ANDRADE NETO, D. M.; RODRIGUES, M. DO L. L.; LIMA, H. L. S.; PAIVA, D. V. M.; SILVA, M. A. S. DA; FECHINE, L. M. U. D.; SOMBRA, A. S. B.; FREIRE, R. M.; DENARDIN, J. C.; ROSA, M. de F.; SOUZA FILHO, M. de S. M. de; MAZZETTO, S. E.; FECHINE, P. B. A. |
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Afiliação: |
FERNANDO LIMA DE MENEZES, Group of Chemistry of Advanced Materials (GQMat)–Department of Analytical Chemistry and Physic-Chemistry, Federal University of Ceará; DAVINO MACHADO ANDRADE NETO, Group of Chemistry of Advanced Materials (GQMat)–Department of Analytical Chemistry and Physic-Chemistry, Federal University of Ceará; IFCE- Camocim; MARIA DO LIVRAMENTO LINHARES RODRIGUES, Department of Organic and Inorganic Chemistry–Federal University of Ceará; HELDER LEVI SILVA LIMA, Department of Chemical Engineering–Federal University of Ceará; DENIS VALONY MARTINS PAIVA, Department of Organic and Inorganic Chemistry–Federal University of Ceará; MARCELO ANTÔNIO SANTOS DA SILVA, Laboratory of Telecommunications and Science and Materials Engineering (LOCEM)–Department of Physics, Federal University of Ceará; LILLIAN MARIA UCHÔA DUTRA FECHINE, Group of Chemistry of Advanced Materials (GQMat)–Department of Analytical Chemistry and Physic-Chemistry, Federal University of Ceará; ANTÔNIO SÉRGIO BEZERRA SOMBRA, oratory of Telecommunications and Science and Materials Engineering (LOCEM)–Department of Physics, Federal University of Ceará; RAFAEL MELO FREIRE, Institute of Applied Chemical Sciences, Universidad Autónoma de Chile, Santiago; JULIANO CASAGRANDE DENARDIN, Departamento de Física/CEDENNA, Universidad de Santiago de Chile, USACH; MORSYLEIDE DE FREITAS ROSA, CNPAT; MEN DE SA MOREIRA DE SOUZA FILHO, CNPAT; SELMA ELAINE MAZZETTO, Laboratory of Products and Process Technology (LPT), Department of Organic and Inorganic Chemistry, Federal University of Ceará; PIERRE BASÍLIO ALMEIDA FECHINE, Group of Chemistry of Advanced Materials (GQMat)?Department of Analytical Chemistry and Physic-Chemistry, Federal University of Ceará. |
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Título: |
From magneto-dielectric biocomposite films to microstrip antenna devices. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Journal of Composites Science, v. 4, n. 4, art. n. 144, 2020. |
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DOI: |
doi:10.3390/jcs4040144 |
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Idioma: |
Inglês |
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Palavras-Chave: |
Antenas microstrip; Biopolímeros; Magnetic-dielectric property; Microstrip antenas; Nanocompósitos; Propriedade magnético-dielétrica. |
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Thesaurus NAL: |
Biopolymers; Nanocomposites. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01145naa a2200373 a 4500
001 2132053
005 2021-06-30
008 2020 bl uuuu u00u1 u #d
024 7 $adoi:10.3390/jcs4040144$2DOI
100 1 $aMENEZES, F. L. DE
245 $aFrom magneto-dielectric biocomposite films to microstrip antenna devices.$h[electronic resource]
260 $c2020
650 $aBiopolymers
650 $aNanocomposites
653 $aAntenas microstrip
653 $aBiopolímeros
653 $aMagnetic-dielectric property
653 $aMicrostrip antenas
653 $aNanocompósitos
653 $aPropriedade magnético-dielétrica
700 1 $aANDRADE NETO, D. M.
700 1 $aRODRIGUES, M. DO L. L.
700 1 $aLIMA, H. L. S.
700 1 $aPAIVA, D. V. M.
700 1 $aSILVA, M. A. S. DA
700 1 $aFECHINE, L. M. U. D.
700 1 $aSOMBRA, A. S. B.
700 1 $aFREIRE, R. M.
700 1 $aDENARDIN, J. C.
700 1 $aROSA, M. de F.
700 1 $aSOUZA FILHO, M. de S. M. de
700 1 $aMAZZETTO, S. E.
700 1 $aFECHINE, P. B. A.
773 $tJournal of Composites Science$gv. 4, n. 4, art. n. 144, 2020.
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Embrapa Agroindústria Tropical (CNPAT) |
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