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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
14/04/2015 |
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Data da última atualização: |
30/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DORNELES, E. M. S.; SANTANA, J. A.; RIBEIRO, D.; DORELLA, F. A.; GUIMARAES, A. S.; MOAWAD, M. S.; SELIM, S. A.; GARALDI, A. L. M.; MIYOSHI, A.; RIBEIRO, M. G.; GOUVEIA, A. M. G.; AZEVEDO, V.; HEINEMANN, M. B.; LAGE, A. P. |
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Afiliação: |
ELAINE M. S. DORNELES, UFMG; JORDANA A. SANTANA, UFMG; DAYANA RIBEIRO, UFMG; FERNANDA ALVES DORELLA, UFMG; ALESSANDRO DE SA GUIMARAES, CNPGL; MOHAMED S. MOAWAD, Cairo University, Cairo, Egypt; SALAH A. SELIM, Cairo University, Cairo, Egypt; ANA LUIZA M. GARALDI, UNERJ; ANDERSON MIYOSHI, UFMG; MARCIO G. RIBEIRO, UNESP; AURORA M. G. GOUVEIA, UFMG; VASCO AZEVEDO, UFMG; MARCOS B. HEINEMANN, UFMG; ANDREY P. LAGE, UFMG. |
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Título: |
Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Plos One, v. 9, n. 6, e98758, 2014. |
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DOI: |
https://doi.org/10.1371/journal.pone.0098758 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410T ) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations. |
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Palavras-Chave: |
Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR). |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/122336/1/Cnpgl-2014-PlosOne-Evaluation.pdf
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Marc: |
LEADER 02105naa a2200301 a 4500
001 2013504
005 2024-01-30
008 2014 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1371/journal.pone.0098758$2DOI
100 1 $aDORNELES, E. M. S.
245 $aEvaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates.$h[electronic resource]
260 $c2014
520 $aThe aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410T ) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations.
653 $aEnterobacterial Repetitive Intergenic Consensus (ERIC-PCR)
700 1 $aSANTANA, J. A.
700 1 $aRIBEIRO, D.
700 1 $aDORELLA, F. A.
700 1 $aGUIMARAES, A. S.
700 1 $aMOAWAD, M. S.
700 1 $aSELIM, S. A.
700 1 $aGARALDI, A. L. M.
700 1 $aMIYOSHI, A.
700 1 $aRIBEIRO, M. G.
700 1 $aGOUVEIA, A. M. G.
700 1 $aAZEVEDO, V.
700 1 $aHEINEMANN, M. B.
700 1 $aLAGE, A. P.
773 $tPlos One$gv. 9, n. 6, e98758, 2014.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
09/04/2019 |
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Data da última atualização: |
09/04/2019 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
MANTOVANI, E. C.; FRANÇA, G. E.; OLIVEIRA, A. C. de; SVERZUT, C. B.; ANDRADE, C. de L. T. de; SANTOS, W. |
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Afiliação: |
EVANDRO CHARTUNI MANTOVANI, CNPMS; ANTONIO CARLOS DE OLIVEIRA, CNPMS; Universidade Estadual de Campinas; CAMILO DE LELIS TEIXEIRA DE ANDRADE, CNPMS; AGCO Brasil. |
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Título: |
Effects of chemical and physical soil properties spatial variability analysis on two-years no-tillage system corn productivity map in Brazil. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
In: ASAE ANNUAL INTERNATIONAL MEETING, 2001, Sacramento. Technical paper... St.Joseph: ASAE, 2001. |
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Idioma: |
Inglês |
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Conteúdo: |
The field data showed on this 1st year (1999/2000) great differences on com productivity, from 3.2 to 10.6 t/ha, on the same experimental area, of 38 ha under a central pivot irrigation system. Soils maps of chemical (plí, OM, P, K) and physical aspects were made in order to e"..plain these variability's. Comparing the soil chemical maps with com productivity mal' on this 1st year, the results indicate same pattem or similar distribution with' organic matter map. The physical properties maps are being processed now but they are showing a slight correlation of low com productivity area with soil compaction. On this second year (2000/2001), half of the experimental area was planted with soybean and half with com, trying to use similar situation no-tillage system ofthe Center-West region of Brazil. |
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Palavras-Chave: |
Variabilidade espacial. |
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Thesagro: |
Agricultura de Precisão; Fertilidade do Solo; Fósforo; Milho; Plantio Direto; Produtividade. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/195636/1/Effects-chemical.pdf
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Marc: |
LEADER 01662nam a2200253 a 4500
001 2108035
005 2019-04-09
008 2001 bl uuuu u00u1 u #d
100 1 $aMANTOVANI, E. C.
245 $aEffects of chemical and physical soil properties spatial variability analysis on two-years no-tillage system corn productivity map in Brazil.$h[electronic resource]
260 $aIn: ASAE ANNUAL INTERNATIONAL MEETING, 2001, Sacramento. Technical paper... St.Joseph: ASAE$c2001
520 $aThe field data showed on this 1st year (1999/2000) great differences on com productivity, from 3.2 to 10.6 t/ha, on the same experimental area, of 38 ha under a central pivot irrigation system. Soils maps of chemical (plí, OM, P, K) and physical aspects were made in order to e"..plain these variability's. Comparing the soil chemical maps with com productivity mal' on this 1st year, the results indicate same pattem or similar distribution with' organic matter map. The physical properties maps are being processed now but they are showing a slight correlation of low com productivity area with soil compaction. On this second year (2000/2001), half of the experimental area was planted with soybean and half with com, trying to use similar situation no-tillage system ofthe Center-West region of Brazil.
650 $aAgricultura de Precisão
650 $aFertilidade do Solo
650 $aFósforo
650 $aMilho
650 $aPlantio Direto
650 $aProdutividade
653 $aVariabilidade espacial
700 1 $aFRANÇA, G. E.
700 1 $aOLIVEIRA, A. C. de
700 1 $aSVERZUT, C. B.
700 1 $aANDRADE, C. de L. T. de
700 1 $aSANTOS, W.
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Embrapa Milho e Sorgo (CNPMS) |
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