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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
26/09/2019 |
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Data da última atualização: |
07/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AZEVEDO, D. A. A. de; PINHEIRO, R. R.; SANTOS, V. W. S. dos; DAMASCENO, E. M.; SOUSA, A. L. M. de; ARAÚJO, J. F.; ANDRIOLI, A.; SIDER, L. H.; PEIXOTO, R. M.; TEIXEIRA, M. F. da S. |
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Afiliação: |
DALVA ALANA ARAGÃO DE AZEVEDO, Pós-graduação - Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brasil; RAYMUNDO RIZALDO PINHEIRO, CNPC; VANDERLAN WARLINGTON SOUZA DOS SANTOS, Pós-graduação - Universidade Federal Rural do Semiárido (UFERSA) - Mossoró, RN, Brasil; EDGAR MARQUES DAMASCENO, Pós-Graduação - Universidade Estadual Vale do Acaraú (UVA), Sobral, CE, Brasil; ANA LÍDIA MADEIRA DE SOUSA; JUSCILÂNIA FURTADO ARAÚJO, Rede de Biotecnologia do Nordeste (RENORBIO), UECE, Fortaleza, CE, Brasil; ALICE ANDRIOLI, CNPC; LUCIA HELENA SIDER, CNPC; RENATO MESQUITA PEIXOTO, Pós-graduação - Universidade Federal do Acre (UFAC) - Rio Branco, AC, Brasil.; MARIA FÁTIMA DA SILVA TEIXEIRA, Pós-graduação - Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brazil. |
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Título: |
Comparação de testes sorológicos e molecular para diagnóstico da Artrite Encefalite Caprina e avaliação clínica da glândula mamária de caprinos leiteiros infectados. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Acta Scientiae Veterinariae, v. 47, Pub. 1668, 2019. |
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Idioma: |
Português |
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Conteúdo: |
Background: Caprine Arthritis Encephalitis (CAE) is a disease that causes productive losses in dairy goat flocks due to the reduction in milk production, followed by lesions in joints and mammary glands. An early diagnosis is essential, considering that there is frequent occurrence of asymptomatic animals. Hence, this study aimed to perform a comparison of immunological and molecular based diagnostic tests, represented by Agar Gel Immunodiffusion (AGID), Western Blot (WB) and nested Polymerase Chain Reaction (nPCR). In addition, the mammary glands (MG) of dairy goats were clinically evaluated. Materials, Methods & Results: Blood collection and clinical examination were performed in 1191 dairy goats of 12 farms located in Northeastern and Southeastern regions of Brazil. Serological (AGID, WB) and molecular (nPCR) test results were compared and the data, along with MG alterations, were analyzed using Epi-info 7 and WinEpiscope 2.0. Seroprevalence in AGID test was 41.14% (490/1191). In WB, 51.47% (613/1191) of animals were seropositive and nPCR detected 69.44% (827/1191) positive animals. Hence, WB was more sensitive (P < 0.001) than AGID. However, nPCR detected more positive animals than AGID (P < 0.001) and WB (P < 0.001). The analysis of mammary glands revealed that 105 out of 1096 nanny goats presented alterations, of which 101 presented altered consistency, 16 presented elevated temperatures and 60 had enlarged retromammary lymph nodes. There was significant statistic difference (P < 0.05) only when comparing the results of serological tests with MG alterations. Discussion: In general, AGID technique is most frequently used when screening flocks for the disease due to the practicality and low cost this test presents. However, the results demonstrated that AGID detected the lowest number of positive animals. This low sensitivity that the test presented may be attributed to its antigen-antibody interaction mechanism, considering that agar gel precipitation requires multiple interactions. In addition, WB was more effective than AGID in detecting antibodies. On the other hand, nPCR was important for the detection of infected animals that serological tests failed to detect. The intermittence of immunological response observed in the serological tests may be explained by the variation of antibodies levels that may occur during life. Likewise, viral compartmentalization would justify the intermittent detection of proviral DNA. Hence, the results can be influenced by the viral intermittence, test sensitivity, late seroconversion and statistic values that can be calculated (sensitivity, specificity, positive predictive value, negative predictive level and kappa). Crossing the results of the diagnostic tests with the different mammary gland alterations, it was shown that there was a statistically significant difference (P <0.05) only in the comparison of the results of the serological tests with GM alterations. Everything indicates that the humoral or cellular immune system being on stimulus is more propitious to find these changes. In conclusion, WB was more sensitive than AGID and, considering that nPCR can detect a larger number of animals infected with the goat lentivirus, it must be associated with a sensible serological test, such as Western Blot. In addition, infected animals have alterations in MG, which is more frequent in cases with positive serological. [Comparison of serological and molecular tests for diagnosis of caprine arthritis encephalitis and clinical evaluation of mammary glands of infected dairy goats]. MenosBackground: Caprine Arthritis Encephalitis (CAE) is a disease that causes productive losses in dairy goat flocks due to the reduction in milk production, followed by lesions in joints and mammary glands. An early diagnosis is essential, considering that there is frequent occurrence of asymptomatic animals. Hence, this study aimed to perform a comparison of immunological and molecular based diagnostic tests, represented by Agar Gel Immunodiffusion (AGID), Western Blot (WB) and nested Polymerase Chain Reaction (nPCR). In addition, the mammary glands (MG) of dairy goats were clinically evaluated. Materials, Methods & Results: Blood collection and clinical examination were performed in 1191 dairy goats of 12 farms located in Northeastern and Southeastern regions of Brazil. Serological (AGID, WB) and molecular (nPCR) test results were compared and the data, along with MG alterations, were analyzed using Epi-info 7 and WinEpiscope 2.0. Seroprevalence in AGID test was 41.14% (490/1191). In WB, 51.47% (613/1191) of animals were seropositive and nPCR detected 69.44% (827/1191) positive animals. Hence, WB was more sensitive (P < 0.001) than AGID. However, nPCR detected more positive animals than AGID (P < 0.001) and WB (P < 0.001). The analysis of mammary glands revealed that 105 out of 1096 nanny goats presented alterations, of which 101 presented altered consistency, 16 presented elevated temperatures and 60 had enlarged retromammary lymph nodes. There was significant statistic diff... Mostrar Tudo |
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Palavras-Chave: |
CAE; Caprino leiteiro; Detecção; Exame clínico; Lentivírus caprino. |
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Thesagro: |
Cabra Leiteira; Caprino; Doença Animal; Vírus. |
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Thesaurus Nal: |
Caprine arthritis encephalitis virus; Clinical examination; Dairy goats; Detection; Goat diseases; Lentivirus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/202345/1/cnpc-2019-Comparacao.pdf
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Marc: |
LEADER 04823naa a2200409 a 4500
001 2112509
005 2019-11-07
008 2019 bl uuuu u00u1 u #d
100 1 $aAZEVEDO, D. A. A. de
245 $aComparação de testes sorológicos e molecular para diagnóstico da Artrite Encefalite Caprina e avaliação clínica da glândula mamária de caprinos leiteiros infectados.$h[electronic resource]
260 $c2019
520 $aBackground: Caprine Arthritis Encephalitis (CAE) is a disease that causes productive losses in dairy goat flocks due to the reduction in milk production, followed by lesions in joints and mammary glands. An early diagnosis is essential, considering that there is frequent occurrence of asymptomatic animals. Hence, this study aimed to perform a comparison of immunological and molecular based diagnostic tests, represented by Agar Gel Immunodiffusion (AGID), Western Blot (WB) and nested Polymerase Chain Reaction (nPCR). In addition, the mammary glands (MG) of dairy goats were clinically evaluated. Materials, Methods & Results: Blood collection and clinical examination were performed in 1191 dairy goats of 12 farms located in Northeastern and Southeastern regions of Brazil. Serological (AGID, WB) and molecular (nPCR) test results were compared and the data, along with MG alterations, were analyzed using Epi-info 7 and WinEpiscope 2.0. Seroprevalence in AGID test was 41.14% (490/1191). In WB, 51.47% (613/1191) of animals were seropositive and nPCR detected 69.44% (827/1191) positive animals. Hence, WB was more sensitive (P < 0.001) than AGID. However, nPCR detected more positive animals than AGID (P < 0.001) and WB (P < 0.001). The analysis of mammary glands revealed that 105 out of 1096 nanny goats presented alterations, of which 101 presented altered consistency, 16 presented elevated temperatures and 60 had enlarged retromammary lymph nodes. There was significant statistic difference (P < 0.05) only when comparing the results of serological tests with MG alterations. Discussion: In general, AGID technique is most frequently used when screening flocks for the disease due to the practicality and low cost this test presents. However, the results demonstrated that AGID detected the lowest number of positive animals. This low sensitivity that the test presented may be attributed to its antigen-antibody interaction mechanism, considering that agar gel precipitation requires multiple interactions. In addition, WB was more effective than AGID in detecting antibodies. On the other hand, nPCR was important for the detection of infected animals that serological tests failed to detect. The intermittence of immunological response observed in the serological tests may be explained by the variation of antibodies levels that may occur during life. Likewise, viral compartmentalization would justify the intermittent detection of proviral DNA. Hence, the results can be influenced by the viral intermittence, test sensitivity, late seroconversion and statistic values that can be calculated (sensitivity, specificity, positive predictive value, negative predictive level and kappa). Crossing the results of the diagnostic tests with the different mammary gland alterations, it was shown that there was a statistically significant difference (P <0.05) only in the comparison of the results of the serological tests with GM alterations. Everything indicates that the humoral or cellular immune system being on stimulus is more propitious to find these changes. In conclusion, WB was more sensitive than AGID and, considering that nPCR can detect a larger number of animals infected with the goat lentivirus, it must be associated with a sensible serological test, such as Western Blot. In addition, infected animals have alterations in MG, which is more frequent in cases with positive serological. [Comparison of serological and molecular tests for diagnosis of caprine arthritis encephalitis and clinical evaluation of mammary glands of infected dairy goats].
650 $aCaprine arthritis encephalitis virus
650 $aClinical examination
650 $aDairy goats
650 $aDetection
650 $aGoat diseases
650 $aLentivirus
650 $aCabra Leiteira
650 $aCaprino
650 $aDoença Animal
650 $aVírus
653 $aCAE
653 $aCaprino leiteiro
653 $aDetecção
653 $aExame clínico
653 $aLentivírus caprino
700 1 $aPINHEIRO, R. R.
700 1 $aSANTOS, V. W. S. dos
700 1 $aDAMASCENO, E. M.
700 1 $aSOUSA, A. L. M. de
700 1 $aARAÚJO, J. F.
700 1 $aANDRIOLI, A.
700 1 $aSIDER, L. H.
700 1 $aPEIXOTO, R. M.
700 1 $aTEIXEIRA, M. F. da S.
773 $tActa Scientiae Veterinariae$gv. 47, Pub. 1668, 2019.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Rondônia. |
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Data corrente: |
17/12/2013 |
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Data da última atualização: |
17/12/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
CIPRIANI, H. N.; DIAS, L. E.; COSTA, M. D.; CAMPOS, N. V.; AZEVEDO, A. A.; GOMES, R. J.; FIALHO, I. F.; AMEZQUITA, S. P. M. |
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Afiliação: |
HENRIQUE NERY CIPRIANI, CPAF-RO; Luiz Eduardo Dias, Universidade Federal de Viçosa; Maurício Dutra Costa, Universidade Federal de Viçosa; Naiara Viana Campos, Universidade Federal de Viçosa; Aristéa Alves Azevedo, Universidade Federal de Viçosa; Roberto Junio Gomes, Universidade Federal de Viçosa; Izabela Ferreira Fialho, Universidade Federal de Viçosa; Sandra Patrícia Montealegre Amezquita, Universidade Federal de Viçosa. |
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Título: |
Arsenic toxicity in Acacia mangium WILLD. and Mimosa caesalpiniaefolia BENTH. Seedlings. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Revista Brasileira de Ciência do Solo, Viçosa-MG, v. 37, n.5, p.1423-1430, 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
Acacia mangium and Mimosa caesalpiniaefolia are fast-growing woody fabaceous species that might be suitable for phytoremediation of arsenic (As)- contaminated sites. To date, few studies on their tolerance to As toxicity have been published. Therefore, this study assessed As toxicity symptoms in A. mangium and M. caesalpiniaefolia seedlings under As stress in a greenhouse. Seedlings of Acacia mangium and M. caesalpiniaefolia were grown for 120 d in an Oxisol-sand mixture with 0, 50, 100, 200, and 400 mg kg-1 As, in four replications in four randomized blocks. The plants were assessed for visible toxicity symptoms, dry matter production, shoot/root ratio, root anatomy and As uptake. Analyses of variance and regression showed that the growth of A. mangium and M. caesalpiniaefolia was severely hindered by As, with a reduction in dry matter production of more than 80 % at the highest As rate. The root/shoot ratio increased with increasing As rates. At a rate of 400 mg kg-1 As, whitish chlorosis appeared on Mimosa caesalpiniaefolia seedlings. The root anatomy of both species was altered, resulting in cell collapse, death of root buds and accumulation of phenolic compounds. Arsenic concentration was several times greater in roots than in shoots, with more than 150 and 350 mg kg-1 in M. caesalpiniaefolia and A. mangium roots, respectively. These species could be suitable for phytostabilization of As-contaminated sites, but growth-stimulating measures should be used. Acacia mangium e Mimosa caesalpiniaefolia são fabáceas lenhosas de rápido crescimento cuja tolerância à toxidez por arsênio (As) deve ser estudada para avaliação do potencial de fitorremediação. Assim, este experimento, conduzido em casa de vegetação, objetivou avaliar sintomas de toxidez por As em A. mangium e M. caesalpiniaefolia. Plantas seminais de A. mangium e M. caesalpiniaefolia foram cultivadas por 120 d em uma mistura de Latossolo Vermelho e areia, contaminadas com 0, 50, 100, 200 e 400 mg kg-1 de As. Utilizaram-se quatro repetições distribuídas em quatro blocos casualizados. Foram avaliados os sintomas visíveis de toxidez, a produção de matéria seca, a relação raiz/parte aérea, a anatomia radicular e o acúmulo de As. As análises de variância e de regressão evidenciaram que o crescimento das plantas foi prejudicado pelo As, excedendo 80 % de redução da produção de matéria seca na maior dose. A relação raiz/parte aérea aumentou com a adição de As. Plantas de M. caesalpiniaefolia manifestaram clorose esbranquiçada sob 400 mg kg-1 de As. A anatomia radicular das espécies foi alterada, observando-se células colapsadas, morte de primórdios radiculares e acúmulo de compostos fenólicos. A concentração de As nas raízes foi dezenas de vezes superior à concentração na parte área, excedendo 150 e 350 mg kg-1 na M. caesalpiniaefolia e na A. mangium, respectivamente. Essas espécies apresentaram potencial para fitoestabilização de áreas contaminadas com As, porém devem ser adotadas medidas para promover seu crescimento nesses locais. MenosAcacia mangium and Mimosa caesalpiniaefolia are fast-growing woody fabaceous species that might be suitable for phytoremediation of arsenic (As)- contaminated sites. To date, few studies on their tolerance to As toxicity have been published. Therefore, this study assessed As toxicity symptoms in A. mangium and M. caesalpiniaefolia seedlings under As stress in a greenhouse. Seedlings of Acacia mangium and M. caesalpiniaefolia were grown for 120 d in an Oxisol-sand mixture with 0, 50, 100, 200, and 400 mg kg-1 As, in four replications in four randomized blocks. The plants were assessed for visible toxicity symptoms, dry matter production, shoot/root ratio, root anatomy and As uptake. Analyses of variance and regression showed that the growth of A. mangium and M. caesalpiniaefolia was severely hindered by As, with a reduction in dry matter production of more than 80 % at the highest As rate. The root/shoot ratio increased with increasing As rates. At a rate of 400 mg kg-1 As, whitish chlorosis appeared on Mimosa caesalpiniaefolia seedlings. The root anatomy of both species was altered, resulting in cell collapse, death of root buds and accumulation of phenolic compounds. Arsenic concentration was several times greater in roots than in shoots, with more than 150 and 350 mg kg-1 in M. caesalpiniaefolia and A. mangium roots, respectively. These species could be suitable for phytostabilization of As-contaminated sites, but growth-stimulating measures should be used. Acacia mangium ... Mostrar Tudo |
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Palavras-Chave: |
Anatomia radicular; Arsenate; Contaminação do solo; Fitorremediação; Metais pesados; Root anatomy; Soil contamination. |
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Thesaurus NAL: |
heavy metals; phytoremediation. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/94273/1/31-539.pdf
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Marc: |
LEADER 04014naa a2200313 a 4500
001 1974289
005 2013-12-17
008 2013 bl uuuu u00u1 u #d
100 1 $aCIPRIANI, H. N.
245 $aArsenic toxicity in Acacia mangium WILLD. and Mimosa caesalpiniaefolia BENTH. Seedlings.$h[electronic resource]
260 $c2013
520 $aAcacia mangium and Mimosa caesalpiniaefolia are fast-growing woody fabaceous species that might be suitable for phytoremediation of arsenic (As)- contaminated sites. To date, few studies on their tolerance to As toxicity have been published. Therefore, this study assessed As toxicity symptoms in A. mangium and M. caesalpiniaefolia seedlings under As stress in a greenhouse. Seedlings of Acacia mangium and M. caesalpiniaefolia were grown for 120 d in an Oxisol-sand mixture with 0, 50, 100, 200, and 400 mg kg-1 As, in four replications in four randomized blocks. The plants were assessed for visible toxicity symptoms, dry matter production, shoot/root ratio, root anatomy and As uptake. Analyses of variance and regression showed that the growth of A. mangium and M. caesalpiniaefolia was severely hindered by As, with a reduction in dry matter production of more than 80 % at the highest As rate. The root/shoot ratio increased with increasing As rates. At a rate of 400 mg kg-1 As, whitish chlorosis appeared on Mimosa caesalpiniaefolia seedlings. The root anatomy of both species was altered, resulting in cell collapse, death of root buds and accumulation of phenolic compounds. Arsenic concentration was several times greater in roots than in shoots, with more than 150 and 350 mg kg-1 in M. caesalpiniaefolia and A. mangium roots, respectively. These species could be suitable for phytostabilization of As-contaminated sites, but growth-stimulating measures should be used. Acacia mangium e Mimosa caesalpiniaefolia são fabáceas lenhosas de rápido crescimento cuja tolerância à toxidez por arsênio (As) deve ser estudada para avaliação do potencial de fitorremediação. Assim, este experimento, conduzido em casa de vegetação, objetivou avaliar sintomas de toxidez por As em A. mangium e M. caesalpiniaefolia. Plantas seminais de A. mangium e M. caesalpiniaefolia foram cultivadas por 120 d em uma mistura de Latossolo Vermelho e areia, contaminadas com 0, 50, 100, 200 e 400 mg kg-1 de As. Utilizaram-se quatro repetições distribuídas em quatro blocos casualizados. Foram avaliados os sintomas visíveis de toxidez, a produção de matéria seca, a relação raiz/parte aérea, a anatomia radicular e o acúmulo de As. As análises de variância e de regressão evidenciaram que o crescimento das plantas foi prejudicado pelo As, excedendo 80 % de redução da produção de matéria seca na maior dose. A relação raiz/parte aérea aumentou com a adição de As. Plantas de M. caesalpiniaefolia manifestaram clorose esbranquiçada sob 400 mg kg-1 de As. A anatomia radicular das espécies foi alterada, observando-se células colapsadas, morte de primórdios radiculares e acúmulo de compostos fenólicos. A concentração de As nas raízes foi dezenas de vezes superior à concentração na parte área, excedendo 150 e 350 mg kg-1 na M. caesalpiniaefolia e na A. mangium, respectivamente. Essas espécies apresentaram potencial para fitoestabilização de áreas contaminadas com As, porém devem ser adotadas medidas para promover seu crescimento nesses locais.
650 $aheavy metals
650 $aphytoremediation
653 $aAnatomia radicular
653 $aArsenate
653 $aContaminação do solo
653 $aFitorremediação
653 $aMetais pesados
653 $aRoot anatomy
653 $aSoil contamination
700 1 $aDIAS, L. E.
700 1 $aCOSTA, M. D.
700 1 $aCAMPOS, N. V.
700 1 $aAZEVEDO, A. A.
700 1 $aGOMES, R. J.
700 1 $aFIALHO, I. F.
700 1 $aAMEZQUITA, S. P. M.
773 $tRevista Brasileira de Ciência do Solo, Viçosa-MG$gv. 37, n.5, p.1423-1430, 2013.
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