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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
25/11/2022 |
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Data da última atualização: |
25/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARVALHO, M. H. de; ROSA, S. D. V. F. da; COELHO, S. V. B.; GUIMARÃES, C. C.; MARTINS, R. de S.; CLEMENTE, A. da C. S.; PAIVA, L. V. |
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Afiliação: |
MAYARA HOLANDA DE CARVALHO; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; STEFÂNIA VIIAS BOAS COELHO, UNIVERSIDADE FEDERAL DE LAVRAS; CRISTIANE CARVALHO GUIMARÃES, UNIVERSIDADE ESTADUAL PAULISTA; RAYANA DE SÁ MARTINS, INSTITUTO DE BOTÂNICA; ALINE DA CONSOLAÇÃO SAMPAIO CLEMENTE, ONSEEDS TREINAMENTOS; LUCIANO VILELA PAIVA, UNIVERSIDADE FEDERAL DE LAVRAS. |
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Título: |
Drying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Acta Scientiarum: Agronomy, v. 45, e56908, 2023. |
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DOI: |
https://doi.org/10.4025/actasciagron.v45i1.56908 |
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Idioma: |
Inglês |
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Conteúdo: |
The reduced longevity of coffee seeds has been attributed to their sensitivity to desiccation. Studies related to gene expression and enzyme activity in coffee seeds under drying are important for understanding the effects of drying on their physiological quality. The aim of this study was to investigate the molecular aspects of seeds under different drying methods and associate them with physiological quality. Coffee seeds with different water contents were dried both slowly and rapidly. Enzymatic activity was analysed, as well as the expression of genes that encode the enzymes superoxide dismutase, catalase, peroxiredoxins, isocitrate lyase, and endo-ß-mannanase. There was a significant effect of drying speed and final water content on enzyme activity and on the expression of the different genes analysed. In seeds under rapid drying, there was greater expression of the genes that encode the enzymes catalase and endo-ß-mannanase. Greater expression of the 1 CYS PRX and SOD genes and greater activity of the ICL isoenzymes were found in seeds with superior physiological quality, but greater activity of the endo-β-mannanase and CAT enzymes occurred in seeds with lower physiological quality. |
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Thesaurus Nal: |
Coffea arabica var. arabica; Drying; Enzyme activity; Oxidative stress. |
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Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148771/1/Carvalho-et-al-2023-Drying-of-arabica-coffee-and-its-effect-on-the-gene-expression-and-activity-of-enzymes-linked-to-seed-physiological-quality.pdf
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Marc: |
LEADER 02045naa a2200253 a 4500
001 2148771
005 2022-11-25
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.4025/actasciagron.v45i1.56908$2DOI
100 1 $aCARVALHO, M. H. de
245 $aDrying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality.$h[electronic resource]
260 $c2023
520 $aThe reduced longevity of coffee seeds has been attributed to their sensitivity to desiccation. Studies related to gene expression and enzyme activity in coffee seeds under drying are important for understanding the effects of drying on their physiological quality. The aim of this study was to investigate the molecular aspects of seeds under different drying methods and associate them with physiological quality. Coffee seeds with different water contents were dried both slowly and rapidly. Enzymatic activity was analysed, as well as the expression of genes that encode the enzymes superoxide dismutase, catalase, peroxiredoxins, isocitrate lyase, and endo-ß-mannanase. There was a significant effect of drying speed and final water content on enzyme activity and on the expression of the different genes analysed. In seeds under rapid drying, there was greater expression of the genes that encode the enzymes catalase and endo-ß-mannanase. Greater expression of the 1 CYS PRX and SOD genes and greater activity of the ICL isoenzymes were found in seeds with superior physiological quality, but greater activity of the endo-β-mannanase and CAT enzymes occurred in seeds with lower physiological quality.
650 $aCoffea arabica var. arabica
650 $aDrying
650 $aEnzyme activity
650 $aOxidative stress
700 1 $aROSA, S. D. V. F. da
700 1 $aCOELHO, S. V. B.
700 1 $aGUIMARÃES, C. C.
700 1 $aMARTINS, R. de S.
700 1 $aCLEMENTE, A. da C. S.
700 1 $aPAIVA, L. V.
773 $tActa Scientiarum: Agronomy$gv. 45, e56908, 2023.
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Registro original: |
Embrapa Café (CNPCa) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
28/03/2009 |
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Data da última atualização: |
10/07/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVA-PINHATI, A. C.; BOSCARIOL-CAMARGO, R. L.; MALOSSO, A.; SOUZA, A. A. de; AMARAL, A. M. do; CARLOS, E. F.; FREITAS-ASTUA, J.; TAKITA, M. A.; TARGON, M. L. N. P.; MACHADO, M. A. |
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Afiliação: |
Ana Carla Silva-Pinhati, IAC; Raquel Luciana Boscariol-Camargo, IAC; Adriano Malosso, IAC; Alessandra Alves de Souza, IAC; Alexandre Morais do Amaral, CENARGEN; Eduardo Fermino Carlos, IAC; Juliana Freitas-Astua, CNPMF; Marco Aurelio Takita, IAC; Maria Luisa N.P. Targon, IAC; Marcos Antonio Machado, IAC. |
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Título: |
ESTs of roots of different citrus species and differential expression of genes associated to drought tolerance. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: THE INTERNATIONAL CONFERENCE ON THE STATUS OF PLANT & ANIMAL GENOME RESEARCH, 16., 2008, San Diego. Final abstracts guide. San Diego: Illumina, 2008. |
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Idioma: |
Inglês |
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Conteúdo: |
Biotic and abiotic stresses in citrus species are the main challenges to the breeding programs and to maintain the productivity at field conditions. Among abiotic factors affecting citrus growth water stress represents an important factor around the world, especially in no irrigated citrus orchards. Rangpur lime (Citrus limonia Osb. cv Santa Barbara, tolerant to water stress), Swingle citrumelo (C. paradisi x Poncirus trifoliata, less tolerant to water stress), and trifoliate orange (P. trifoliata cv. Rubidoux, susceptible to water stress) are important rootstocks in Brazil and in other citrus growing areas around the world. Aiming to compare the response to water stress conditions both ESTs libraries and gene expressions were evaluated in these three rootstocks. Libraries of 19,356 ESTs were produced (6325 of Rangpur lime, 7182 of Swingle citrumelo, and 5849 of P. trifoliata), and statistically evaluated to compare the frequency of more expressed sequences, which were used to follow up their expression by RT-qPCR. Roots under induced water stress were used in the biological assays. Selected genes for expression studies included the transcription factor CBF-1, the gene of the enzyme P5CS, germin-like gene, aquaporin, and dehidrin. Analysis of Rangpur lime revealed that germin-like, dehidrin and aquaporin were expressed lately, suggesting no participation in the initial response to the water stress. On the other hand, different response was obtained with p5cs and cbf genes that were quickly activated in Rangpur lime and Swingle citrumelo. These data suggested that transcription factors and proline are involved in initial responses of resistance to drought and could be responsible for the differences involving stress tolerance among the genotypes. MenosBiotic and abiotic stresses in citrus species are the main challenges to the breeding programs and to maintain the productivity at field conditions. Among abiotic factors affecting citrus growth water stress represents an important factor around the world, especially in no irrigated citrus orchards. Rangpur lime (Citrus limonia Osb. cv Santa Barbara, tolerant to water stress), Swingle citrumelo (C. paradisi x Poncirus trifoliata, less tolerant to water stress), and trifoliate orange (P. trifoliata cv. Rubidoux, susceptible to water stress) are important rootstocks in Brazil and in other citrus growing areas around the world. Aiming to compare the response to water stress conditions both ESTs libraries and gene expressions were evaluated in these three rootstocks. Libraries of 19,356 ESTs were produced (6325 of Rangpur lime, 7182 of Swingle citrumelo, and 5849 of P. trifoliata), and statistically evaluated to compare the frequency of more expressed sequences, which were used to follow up their expression by RT-qPCR. Roots under induced water stress were used in the biological assays. Selected genes for expression studies included the transcription factor CBF-1, the gene of the enzyme P5CS, germin-like gene, aquaporin, and dehidrin. Analysis of Rangpur lime revealed that germin-like, dehidrin and aquaporin were expressed lately, suggesting no participation in the initial response to the water stress. On the other hand, different response was obtained with p5cs and cbf genes th... Mostrar Tudo |
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Palavras-Chave: |
Estresse. |
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Thesagro: |
Água; Fruta Cítrica; Gene; Genótipo. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02691nam a2200277 a 4500
001 1655627
005 2023-07-10
008 2008 bl uuuu u00u1 u #d
100 1 $aSILVA-PINHATI, A. C.
245 $aESTs of roots of different citrus species and differential expression of genes associated to drought tolerance.$h[electronic resource]
260 $aIn: THE INTERNATIONAL CONFERENCE ON THE STATUS OF PLANT & ANIMAL GENOME RESEARCH, 16., 2008, San Diego. Final abstracts guide. San Diego: Illumina$c2008
520 $aBiotic and abiotic stresses in citrus species are the main challenges to the breeding programs and to maintain the productivity at field conditions. Among abiotic factors affecting citrus growth water stress represents an important factor around the world, especially in no irrigated citrus orchards. Rangpur lime (Citrus limonia Osb. cv Santa Barbara, tolerant to water stress), Swingle citrumelo (C. paradisi x Poncirus trifoliata, less tolerant to water stress), and trifoliate orange (P. trifoliata cv. Rubidoux, susceptible to water stress) are important rootstocks in Brazil and in other citrus growing areas around the world. Aiming to compare the response to water stress conditions both ESTs libraries and gene expressions were evaluated in these three rootstocks. Libraries of 19,356 ESTs were produced (6325 of Rangpur lime, 7182 of Swingle citrumelo, and 5849 of P. trifoliata), and statistically evaluated to compare the frequency of more expressed sequences, which were used to follow up their expression by RT-qPCR. Roots under induced water stress were used in the biological assays. Selected genes for expression studies included the transcription factor CBF-1, the gene of the enzyme P5CS, germin-like gene, aquaporin, and dehidrin. Analysis of Rangpur lime revealed that germin-like, dehidrin and aquaporin were expressed lately, suggesting no participation in the initial response to the water stress. On the other hand, different response was obtained with p5cs and cbf genes that were quickly activated in Rangpur lime and Swingle citrumelo. These data suggested that transcription factors and proline are involved in initial responses of resistance to drought and could be responsible for the differences involving stress tolerance among the genotypes.
650 $aÁgua
650 $aFruta Cítrica
650 $aGene
650 $aGenótipo
653 $aEstresse
700 1 $aBOSCARIOL-CAMARGO, R. L.
700 1 $aMALOSSO, A.
700 1 $aSOUZA, A. A. de
700 1 $aAMARAL, A. M. do
700 1 $aCARLOS, E. F.
700 1 $aFREITAS-ASTUA, J.
700 1 $aTAKITA, M. A.
700 1 $aTARGON, M. L. N. P.
700 1 $aMACHADO, M. A.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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