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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
06/01/2011 |
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Data da última atualização: |
03/02/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, D. M.; VERLY, R. M.; PILÓ VELOSO, D.; MARIA, M. de; CARVALHO, M. A. R. de; CISALPINO, P. S.; SOARES, B. M.; DINIZ, C. G.; FARIAS, L. M.; MOREIRA, D. F. F.; FRÉZARD, F.; BEMQUERER, M. P.; PIMENTA, A. M. C.; LIMA, M. E. de. |
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Afiliação: |
MARCELO PORTO BEMQUERER, CENARGEN. |
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Título: |
LyeTx I, a potent antimicrobial peptide from the venom of the spider Lycosa erythrognatha. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Amino Acids, v. 39, p. 135-144, 2010. |
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Idioma: |
Inglês |
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Palavras-Chave: |
Antimicrobial peptide; Lycosa erythrognatha; LyeTx I; Spider venom. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/181851/1/Santos2010-Article-LyeTxIAPotentAntimicrobialPept.pdf
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Marc: |
LEADER 00897naa a2200313 a 4500
001 1871814
005 2023-02-03
008 2010 bl uuuu u00u1 u #d
100 1 $aSANTOS, D. M.
245 $aLyeTx I, a potent antimicrobial peptide from the venom of the spider Lycosa erythrognatha.$h[electronic resource]
260 $c2010
653 $aAntimicrobial peptide
653 $aLycosa erythrognatha
653 $aLyeTx I
653 $aSpider venom
700 1 $aVERLY, R. M.
700 1 $aPILÓ VELOSO, D.
700 1 $aMARIA, M. de
700 1 $aCARVALHO, M. A. R. de
700 1 $aCISALPINO, P. S.
700 1 $aSOARES, B. M.
700 1 $aDINIZ, C. G.
700 1 $aFARIAS, L. M.
700 1 $aMOREIRA, D. F. F.
700 1 $aFRÉZARD, F.
700 1 $aBEMQUERER, M. P.
700 1 $aPIMENTA, A. M. C.
700 1 $aLIMA, M. E. de
773 $tAmino Acids$gv. 39, p. 135-144, 2010.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
18/12/2017 |
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Data da última atualização: |
18/06/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
HE, Y.; MATHIEU, J.; YANG, Y.; YU, P.; SILVA, M. L. B. da; ALVAREZ, P. J. J. |
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Afiliação: |
YA HE, Rice University Houston - Texas; JACQUES MATHIEU, Rice University Houston - Texas; YU YANG, Rice University Houston - Texas; PINGFENG YU, Rice University Houston - Texas; MARCIO LUIS BUSI DA SILVA, CNPSA; PEDRO J. J. ALVAREZ, Rice University Houston - Texas. |
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Título: |
Dioxane biodegradation by mycobacterium dioxanotrophicus pH-06 is associated with a group-6 soluble di-iron monooxygenase. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Environmental Science & Technology, v. 4, n. 11, p. 494-499, 2017. |
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DOI: |
10.1021/acs.estlett.7b00456 |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT: 1,4-Dioxane (dioxane) is a groundwater contaminant of emerging concern for which bioremediation may be a promising strategy. Several bacterial strains can metabolize dioxane or degrade it cometabolically. However, the molecular basis of dioxane biodegradation is only partially understood, and the gene coding for dioxane/ tetrahydrofuran (THF) monooxygenase in Pseudonocardia dioxanivorans CB1190 is the only well-characterized catabolic gene. Here, we identify a novel group-6 propane monooxygenase gene cluster (prmABCD) in Mycobacterium dioxanotrophicus PH-06, which is a bacterium with superior dioxane degradation kinetics compared with CB1190. Whole genome sequencing of PH-06 revealed the existence of a single soluble di-iron monooxygenase (SDIMO). RNA sequencing and reverse transcription quantitative PCR (RT-qPCR) subsequently confirmed that all four components of this gene cluster are upregulated when PH-06 is grown on dioxane compared with growth on acetate or glucose as negative controls. This first characterization of a group-6 SDIMO associated with dioxane biodegradation suggests that dioxane-degrading genes may be more diverse than previously appreciated. A primer/probe set designed to target the large hydroxylase subunit of this gene cluster exhibited high selectivity (no false positives) and high sensitivity (detection limit = 3000−4000 gene copies/mL culture), which may be useful to help assess the presence of dioxane degraders at contaminated sites and minimize false negatives. MenosABSTRACT: 1,4-Dioxane (dioxane) is a groundwater contaminant of emerging concern for which bioremediation may be a promising strategy. Several bacterial strains can metabolize dioxane or degrade it cometabolically. However, the molecular basis of dioxane biodegradation is only partially understood, and the gene coding for dioxane/ tetrahydrofuran (THF) monooxygenase in Pseudonocardia dioxanivorans CB1190 is the only well-characterized catabolic gene. Here, we identify a novel group-6 propane monooxygenase gene cluster (prmABCD) in Mycobacterium dioxanotrophicus PH-06, which is a bacterium with superior dioxane degradation kinetics compared with CB1190. Whole genome sequencing of PH-06 revealed the existence of a single soluble di-iron monooxygenase (SDIMO). RNA sequencing and reverse transcription quantitative PCR (RT-qPCR) subsequently confirmed that all four components of this gene cluster are upregulated when PH-06 is grown on dioxane compared with growth on acetate or glucose as negative controls. This first characterization of a group-6 SDIMO associated with dioxane biodegradation suggests that dioxane-degrading genes may be more diverse than previously appreciated. A primer/probe set designed to target the large hydroxylase subunit of this gene cluster exhibited high selectivity (no false positives) and high sensitivity (detection limit = 3000−4000 gene copies/mL culture), which may be useful to help assess the presence of dioxane degraders at contaminated sites ... Mostrar Tudo |
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Palavras-Chave: |
Biodegradação de dioxano; Microbactéria. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/198674/1/marcio-busi.pdf
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Marc: |
LEADER 02224naa a2200217 a 4500
001 2082755
005 2019-06-18
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1021/acs.estlett.7b00456$2DOI
100 1 $aHE, Y.
245 $aDioxane biodegradation by mycobacterium dioxanotrophicus pH-06 is associated with a group-6 soluble di-iron monooxygenase.$h[electronic resource]
260 $c2017
520 $aABSTRACT: 1,4-Dioxane (dioxane) is a groundwater contaminant of emerging concern for which bioremediation may be a promising strategy. Several bacterial strains can metabolize dioxane or degrade it cometabolically. However, the molecular basis of dioxane biodegradation is only partially understood, and the gene coding for dioxane/ tetrahydrofuran (THF) monooxygenase in Pseudonocardia dioxanivorans CB1190 is the only well-characterized catabolic gene. Here, we identify a novel group-6 propane monooxygenase gene cluster (prmABCD) in Mycobacterium dioxanotrophicus PH-06, which is a bacterium with superior dioxane degradation kinetics compared with CB1190. Whole genome sequencing of PH-06 revealed the existence of a single soluble di-iron monooxygenase (SDIMO). RNA sequencing and reverse transcription quantitative PCR (RT-qPCR) subsequently confirmed that all four components of this gene cluster are upregulated when PH-06 is grown on dioxane compared with growth on acetate or glucose as negative controls. This first characterization of a group-6 SDIMO associated with dioxane biodegradation suggests that dioxane-degrading genes may be more diverse than previously appreciated. A primer/probe set designed to target the large hydroxylase subunit of this gene cluster exhibited high selectivity (no false positives) and high sensitivity (detection limit = 3000−4000 gene copies/mL culture), which may be useful to help assess the presence of dioxane degraders at contaminated sites and minimize false negatives.
653 $aBiodegradação de dioxano
653 $aMicrobactéria
700 1 $aMATHIEU, J.
700 1 $aYANG, Y.
700 1 $aYU, P.
700 1 $aSILVA, M. L. B. da
700 1 $aALVAREZ, P. J. J.
773 $tEnvironmental Science & Technology$gv. 4, n. 11, p. 494-499, 2017.
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Embrapa Suínos e Aves (CNPSA) |
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