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| 2. |  | FALEIRO, F. G.; KANNAN, B.; ALTPETER, F. Regeneration of fertile, hexaploid, interspecific hybrids of elephantgrass and pearl millet following treatment of embryogenic calli with antimitotic agents. Plant Cell, Tissue and Organ Culture, v. 124, n. 1, p. 57-67, January 2016.| Biblioteca(s): Embrapa Cerrados. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
16/11/2021 |
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Data da última atualização: |
04/04/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
FALEIRO, F. G.; KIM, J. Y.; PARIKH, A.; ALTPETER, F. |
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Afiliação: |
FABIO GELAPE FALEIRO, CPAC. |
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Título: |
Generation of Transgenic Napier Grass (Cenchrus purpureus Schum.) Plants by Biolistic Gene Transfer of a Minimal Expression Cassette. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Tropical Plant Biology, Sep. 2021. |
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Idioma: |
Inglês |
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Conteúdo: |
Napier grass (Cenchrus purpureus Schum.) is one of the highest yielding tropical C4 forage grasses and a promising candidate feedstock for the production of biofuel. Genetic engineering and genome editing have tremendous potential for crop improvement and require the establishment of a transformation protocol. In this study, transgenic Napier grass was generated by introducing a minimal cassette (MC) encoding the antibiotic resistance gene nptII with biolistic gene transfer into embryogenic callus. Paromomycin was superior to geneticin as a selective agent in the selection of transgenic calli. Two high-biomass producing Napier grass accessions were successfully transformed. Southern blot analysis revealed simple transgene integration patterns with 33% of the regenerated transgenic lines having a single insert of the minimal transgene expression cassette. NPTII-ELISA confirmed transgene expression in all transgenic lines. The results of this study will enable the acceleration of Napier grass improvement by genetic transformation or genome editing. |
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Thesagro: |
Capim Napier; Organismo Transgênico. |
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Thesaurus NAL: |
Genetic engineering; Transgenic plants. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01698naa a2200205 a 4500
001 2136186
005 2023-04-04
008 2021 bl uuuu u00u1 u #d
100 1 $aFALEIRO, F. G.
245 $aGeneration of Transgenic Napier Grass (Cenchrus purpureus Schum.) Plants by Biolistic Gene Transfer of a Minimal Expression Cassette.$h[electronic resource]
260 $c2021
520 $aNapier grass (Cenchrus purpureus Schum.) is one of the highest yielding tropical C4 forage grasses and a promising candidate feedstock for the production of biofuel. Genetic engineering and genome editing have tremendous potential for crop improvement and require the establishment of a transformation protocol. In this study, transgenic Napier grass was generated by introducing a minimal cassette (MC) encoding the antibiotic resistance gene nptII with biolistic gene transfer into embryogenic callus. Paromomycin was superior to geneticin as a selective agent in the selection of transgenic calli. Two high-biomass producing Napier grass accessions were successfully transformed. Southern blot analysis revealed simple transgene integration patterns with 33% of the regenerated transgenic lines having a single insert of the minimal transgene expression cassette. NPTII-ELISA confirmed transgene expression in all transgenic lines. The results of this study will enable the acceleration of Napier grass improvement by genetic transformation or genome editing.
650 $aGenetic engineering
650 $aTransgenic plants
650 $aCapim Napier
650 $aOrganismo Transgênico
700 1 $aKIM, J. Y.
700 1 $aPARIKH, A.
700 1 $aALTPETER, F.
773 $tTropical Plant Biology, Sep. 2021.
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Embrapa Cerrados (CPAC) |
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