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| Registros recuperados : 29 | |
| 21. |  | ALMEIDA, L. B. F.; ZANETE, R. De S. S.; GERN, J. C.; BRANDAO, H. de M.; LADEIRA, L. O.; PEREIRA, M. M. Avaliação in vitro da citotoxicidade de nanotubos de carbono multicamadas carboxilados em fibroblastos bovinos In: SIMPÓSIO NACIONAL DE NANOBIOTECNOLOGIA, 1.; WORKSHOP DE NANOBIOTECNOLOGIA DA UFMG, 2., 2016, Belo Horizonte. Avanços e aplicações: anais. Belo Horizonte: Universidade Federal de Minas Gerais, 2016. p. 83-84.| Biblioteca(s): Embrapa Gado de Leite. |
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| 22. | | ALMEIDA, L. B. de; RAAD, S. M.; FARIAS, M. F. dos S.; MONTEIRO, E. M. M.; LOURENÇO JUNIOR, J. de B.; BRASIL, E. C. Teores de macronutrientes no latossolo amarelo distrófico adubado com resíduo orgânico de siderurgica e fósforo. In: SEMINÁRIO ANUAL DE INICIAÇÃO CIENTÍFICA DA UFRA, 13., 2015, Belém, PA. Anais. Belém, PA: Universidade Federal Rural da Amazônia, 2015.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 23. | | LIMA, A. S.; TRANCOSO, F. O.; MOURA, K. M.; ALMEIDA, L. B.; SILVA, T. N. S.; SOUZA, W. M.; MARCELLINI, P. S. Caracterização centesimal de maxixe e sua aplicação na produção de picles. Alimentos e Nutrição, Araraquara, v. 17, n. 4, p. 407-412, set. 2006.| Biblioteca(s): Embrapa Hortaliças. |
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| 24. | | VIANELLO, J.; BATALHA, B. G.; ALMEIDA, C. G.; SANTA, H. S. D.; MUNK, M.; ALMEIDA, L. B. F. de; SOVRANI, V.; ORONATO, G. de C.; CAPPA, L. G.; BRANDAO, H. de M.; GERN, J. C. Produção e caracterização de exopolissacarídeos produzidos por Ganoderma lucidum e seus estudos toxicológicos. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 24., 2019, Juiz de Fora. Anais... Juiz de Fora: Embrapa Gado de Leite, 2019. 4 p. Editor Técnico: Leônidas Paixão Passos, Embrapa Gado de Leite.| Biblioteca(s): Embrapa Gado de Leite. |
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| 25. | | ZANETTE, R. S. S.; ALMEIDA, L. B. F. DE; SOUZA, N. L. G. D.; ALMEIDA, C. G. DE; OLIVEIRA, L. F. C. DE; MATOS, ELYABE M. DE; GERN, J. C.; BRANDAO, H. de M.; MUNK, M. Cotton cellulose nanofiber/chitosan nanocomposite: characterization and evaluation of cytocompatibility. Journal of Biomaterials Science, Polymer Edition, v. 30, n. 16, p. 1489-1504, 2019.| Biblioteca(s): Embrapa Gado de Leite. |
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| 26. | | DUARTE, J. M. B.; PIOVEZAN, U.; ZANETI, E. dos S.; RAMOS, H. G. da C.; TIEPOLO, L. M.; VOGLIOTTI, A.; OLIVEIRA, M. L. de; RODRIGUES, L. F.; ALMEIDA, L. B. de. Blastocerus dichotomus (Illger, 1815). In: INSTITUTO CHICO MENDES DE CONSERVAÇÃO DA BIODIVERSIDADE. Livro vermelho da fauna brasileira ameaçada de extinção: volume VII - invertebrados. Brasília, DF, 2018. p. 68-73.| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 27. | | BRANDAO, H. de M.; SILVA, S. R.; PEREIRA, M. M.; ALMEIDA, L. B. F.; ZANETTE, R. S. S.; ANDRADE, P. V.; REIGOSA, I. F.; GERN, J. C.; MOSQUEIRA, V. C. F. Avaliação da citotoxicidade da cloxacilina benzatínica nanoestruturada In: SIMPÓSIO NACIONAL DE NANOBIOTECNOLOGIA, 1.; WORKSHOP DE NANOBIOTECNOLOGIA DA UFMG, 2., 2016, Belo Horizonte. Avanços e aplicações: anais. Belo Horizonte: Universidade Federal de Minas Gerais, 2016. p. 84-85.| Biblioteca(s): Embrapa Gado de Leite. |
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| 28. | | PEREIRA, M. M.; BRANDAO, H. de M.; ZANETTE, R. de S. S.; QUINTAO, C. C. R.; SOUZA, E. D. De; ALMEIDA, L. B. F.; LADEIRA, L. O.; CAMARGO, L. S. de A. Transfecção gênica de células primárias de fibroblastos bovinos utilizando nanotubos de carbono multicamadas carboxilados. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 30., 2016, Foz do Iguaçu. Anais... Foz do Iguaçu: Sociedade Brasileira de Tecnologia de Embriões, 2016.| Biblioteca(s): Embrapa Gado de Leite. |
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| 29. | | AMARAL, D. L.; ZANETTE, R. S.; ALMEIDA, C. G.; ALMEIDA, L. B.; OLIVEIRA, L. F.; MARCOMINI, R. F.; NOGUEIRA, B. V.; SANTOS, M. O.; BRANDAO, H. de M.; MARANDUBA, C. M. C.; MUNK, M. In vitro evaluation of barium titanate nanoparticle/alginate 3D scaffold for osteogenic human stem cell differentiation. Biomedical Materials, v. 14, article 035011, 2019.| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 29 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
13/01/2020 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
AMARAL, D. L.; ZANETTE, R. S.; ALMEIDA, C. G.; ALMEIDA, L. B.; OLIVEIRA, L. F.; MARCOMINI, R. F.; NOGUEIRA, B. V.; SANTOS, M. O.; BRANDAO, H. de M.; MARANDUBA, C. M. C.; MUNK, M. |
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Afiliação: |
HUMBERTO DE MELLO BRANDAO, CNPGL. |
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Título: |
In vitro evaluation of barium titanate nanoparticle/alginate 3D scaffold for osteogenic human stem cell differentiation. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Biomedical Materials, v. 14, article 035011, 2019. |
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DOI: |
https://doi.org/10.1088/1748-605x/ab0a52 |
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Idioma: |
Inglês |
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Conteúdo: |
Nanomaterials can mimic properties of extracellular matrix molecules, promising great potential for scaffold composition in tissue engineering. In the present study, we investigated whether barium titanate nanoparticles (BT NP) combined with alginate polymer would provide a new cytocompatible three-dimensional (3D) scaffold to induce osteogenic stem cell differentiation. In vitro cytocompatibility and osteogenic differentiation potential were investigated using human mesenchymal stem cells (MSC). Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays. Overall, neither pure BT NP or BT NP/alginate 3D scaffold induced cytotoxicity. The scanning electron and atomic force microscopy revealed that BT NP/alginate 3D scaffold produced exhibited highly interconnected pores and surface nanotopography that were favorable for MSC differentiation. Von Kossa staining showed mineralization nodules and MSCs morphology changed from spindle to cuboid shape after 21 d. Finally, BMP-2 and ALP mRNA were significantly upregulated on cells grown into the BT NP/alginate 3D scaffold. Thus, the BT NP/alginate 3D scaffold showed an osteogenic differentiation induction potential, without the addition of osteogenic supplements. These results indicate that the BT NP/alginate 3D scaffold provides a cytocompatible and bioactive microenvironment for osteogenic human MSC differentiation. MenosNanomaterials can mimic properties of extracellular matrix molecules, promising great potential for scaffold composition in tissue engineering. In the present study, we investigated whether barium titanate nanoparticles (BT NP) combined with alginate polymer would provide a new cytocompatible three-dimensional (3D) scaffold to induce osteogenic stem cell differentiation. In vitro cytocompatibility and osteogenic differentiation potential were investigated using human mesenchymal stem cells (MSC). Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays. Overall, neither pure BT NP or BT NP/alginate 3D scaffold induced cytotoxicity. The scanning electron and atomic force microscopy revealed that BT NP/alginate 3D scaffold produced exhibited highly interconnected pores and surface nanotopography that were favorable for MSC differentiation. Von Kossa staining showed mineralization nodules and MSCs morphology changed from spindle to cuboid shape after 21 d. Finally, BMP-2 and ALP mRNA were significantly upregulated on cells grown into the BT NP/alginate 3D scaffold. Thus, the BT NP/alginate 3D scaffold showed an osteogenic differentiation induction potential, without the addition of osteogenic supplements. These results indicate that the BT NP/alginate 3D scaffold provides a cytocompatible and bioactive microenvironment for osteogenic... Mostrar Tudo |
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Palavras-Chave: |
3D scaffolds; Human stem cells; Nanomaterial; Nanotopography; Osteoinduction. |
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Categoria do assunto: |
-- |
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Marc: |
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024 7 $ahttps://doi.org/10.1088/1748-605x/ab0a52$2DOI
100 1 $aAMARAL, D. L.
245 $aIn vitro evaluation of barium titanate nanoparticle/alginate 3D scaffold for osteogenic human stem cell differentiation.$h[electronic resource]
260 $c2019
520 $aNanomaterials can mimic properties of extracellular matrix molecules, promising great potential for scaffold composition in tissue engineering. In the present study, we investigated whether barium titanate nanoparticles (BT NP) combined with alginate polymer would provide a new cytocompatible three-dimensional (3D) scaffold to induce osteogenic stem cell differentiation. In vitro cytocompatibility and osteogenic differentiation potential were investigated using human mesenchymal stem cells (MSC). Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays. Overall, neither pure BT NP or BT NP/alginate 3D scaffold induced cytotoxicity. The scanning electron and atomic force microscopy revealed that BT NP/alginate 3D scaffold produced exhibited highly interconnected pores and surface nanotopography that were favorable for MSC differentiation. Von Kossa staining showed mineralization nodules and MSCs morphology changed from spindle to cuboid shape after 21 d. Finally, BMP-2 and ALP mRNA were significantly upregulated on cells grown into the BT NP/alginate 3D scaffold. Thus, the BT NP/alginate 3D scaffold showed an osteogenic differentiation induction potential, without the addition of osteogenic supplements. These results indicate that the BT NP/alginate 3D scaffold provides a cytocompatible and bioactive microenvironment for osteogenic human MSC differentiation.
653 $a3D scaffolds
653 $aHuman stem cells
653 $aNanomaterial
653 $aNanotopography
653 $aOsteoinduction
700 1 $aZANETTE, R. S.
700 1 $aALMEIDA, C. G.
700 1 $aALMEIDA, L. B.
700 1 $aOLIVEIRA, L. F.
700 1 $aMARCOMINI, R. F.
700 1 $aNOGUEIRA, B. V.
700 1 $aSANTOS, M. O.
700 1 $aBRANDAO, H. de M.
700 1 $aMARANDUBA, C. M. C.
700 1 $aMUNK, M.
773 $tBiomedical Materials$gv. 14, article 035011, 2019.
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