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| Registros recuperados : 54 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
18/08/2020 |
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Data da última atualização: |
10/09/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
TEIXEIRA, W. F. A.; BATISTA, R. D.; SANTOS, C. C. A. do A.; FREITAS JÚNIOR, A. C. F.; TERRASAN, C. R. F.; SANTANA, M. W. P. R. de; SIQUEIRA, F. G. de; PAULA-ELIAS, F. C. de; ALMEIDA, A. F. de. |
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Afiliação: |
Wéllen Fabrícia Aviz Teixeira, Universidade Federal do Tocantins; Ryhára Dias Batista, Universidade Federal do Tocantins; Claudia Cristina Auler do Amaral Santos, Universidade Federal do Tocantins; Aloísio Chagas Freitas Júnior, Universidade Federal do Tocantins; César Rafael Fanchini Terrasan, Universidade Estadual Paulista; Marcio Waluce Pinheiro R. de Santana, Universidade Federal da Bahia; FELIX GONCALVES DE SIQUEIRA, CNPAE; Fabrício Coutinho de Paula-Elias, Universidade Federal do Tocantins; Alex Fernando de Almeida, Universidade Federal do Tocantins. |
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Título: |
Minimal Enzymes Cocktail Development by Filamentous Fungi Consortia in Solid-State Cultivation and Valorization of Pineapple Crown Waste by Enzymatic Saccharification. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Waste Biomass Valorization, 2020. |
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ISSN: |
1877-265X |
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DOI: |
https://doi.org/10.1007/s12649-020-01199-8 |
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Idioma: |
Inglês |
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Notas: |
On-line first. Published online: 11 August 2020. |
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Conteúdo: |
Agroindustrial wastes is an interesting opportunity for enzymes production, reducing operational costs and strengthening the biorefineries concept. The conversion of lignocellulosic waste to fermentable sugars is current challenge for biorefineries industries. Enzyme cocktail used in delignification and saccharification platform are produced by a combinatory of several lignocellulolytic enzymes. The aim of this study was to develop filamentous fungi compatible-consortia isolated from pineapple wastes for cellulose-degrading enzymes production using pineapple crown waste in solid-state cultivation and its potential for saccharification of pineapple crown waste. Isolates were screened in agar-plate and in solid-state cultivation for cellulases, xylanase and pectinases productions. Six compatible consortia of Trichoderma strains with Aspergillus niger or Pleurotus ostreatus were evaluated for SSC using pineapple crown. All consortia increased the enzymes production compared to monoculture. Consortia of Trichoderma asperellum PEC-6 and P. ostreatus increased 1.60-fold the xylanase and 1.42-fold the Beta-glucosidase productions. T. asperellum PEC-17 and A. niger increased 1.16-fold endoglucanase and 1.28-fold pectinase. Saccharification of pineapple crown waste using enzyme cocktail produced by filamentous fungi consortia increased the total reducing sugar released in 12.50–13.93% compared to enzymes produced by monocultures. This study provides an alternative model to the cocktail enzyme production by mixed cultures development with lower cost of on-site enzyme manufacture by use of agroindustrial wastes eliminating an enzyme blended step. Moreover, the procedure used in this work can be potentially cost saving and environmentally friendly and should be explored on other bioenergy feedstocks and feed production. MenosAgroindustrial wastes is an interesting opportunity for enzymes production, reducing operational costs and strengthening the biorefineries concept. The conversion of lignocellulosic waste to fermentable sugars is current challenge for biorefineries industries. Enzyme cocktail used in delignification and saccharification platform are produced by a combinatory of several lignocellulolytic enzymes. The aim of this study was to develop filamentous fungi compatible-consortia isolated from pineapple wastes for cellulose-degrading enzymes production using pineapple crown waste in solid-state cultivation and its potential for saccharification of pineapple crown waste. Isolates were screened in agar-plate and in solid-state cultivation for cellulases, xylanase and pectinases productions. Six compatible consortia of Trichoderma strains with Aspergillus niger or Pleurotus ostreatus were evaluated for SSC using pineapple crown. All consortia increased the enzymes production compared to monoculture. Consortia of Trichoderma asperellum PEC-6 and P. ostreatus increased 1.60-fold the xylanase and 1.42-fold the Beta-glucosidase productions. T. asperellum PEC-17 and A. niger increased 1.16-fold endoglucanase and 1.28-fold pectinase. Saccharification of pineapple crown waste using enzyme cocktail produced by filamentous fungi consortia increased the total reducing sugar released in 12.50–13.93% compared to enzymes produced by monocultures. This study provides an alternative model to the cockta... Mostrar Tudo |
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Palavras-Chave: |
Biomass saccharification; Filamentous Fungi Isolation; Microbial consortia; Resíduos lignocelulósicos; Solid-state cultivation. |
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Thesagro: |
Abacaxi; Bioenergia; Biomassa; Enzima; Resíduo Industrial; Trichoderma. |
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Thesaurus NAL: |
Agricultural wastes; Aspergillus; Bioenergy industry; Biomass; Enzymes; Industrial wastes; Lignocellulosic wastes; Microbial culture; Pineapples; Trichoderma asperellum. |
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Categoria do assunto: |
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Marc: |
LEADER 03430naa a2200505 a 4500
001 2124402
005 2020-09-10
008 2020 bl uuuu u00u1 u #d
022 $a1877-265X
024 7 $ahttps://doi.org/10.1007/s12649-020-01199-8$2DOI
100 1 $aTEIXEIRA, W. F. A.
245 $aMinimal Enzymes Cocktail Development by Filamentous Fungi Consortia in Solid-State Cultivation and Valorization of Pineapple Crown Waste by Enzymatic Saccharification.$h[electronic resource]
260 $c2020
500 $aOn-line first. Published online: 11 August 2020.
520 $aAgroindustrial wastes is an interesting opportunity for enzymes production, reducing operational costs and strengthening the biorefineries concept. The conversion of lignocellulosic waste to fermentable sugars is current challenge for biorefineries industries. Enzyme cocktail used in delignification and saccharification platform are produced by a combinatory of several lignocellulolytic enzymes. The aim of this study was to develop filamentous fungi compatible-consortia isolated from pineapple wastes for cellulose-degrading enzymes production using pineapple crown waste in solid-state cultivation and its potential for saccharification of pineapple crown waste. Isolates were screened in agar-plate and in solid-state cultivation for cellulases, xylanase and pectinases productions. Six compatible consortia of Trichoderma strains with Aspergillus niger or Pleurotus ostreatus were evaluated for SSC using pineapple crown. All consortia increased the enzymes production compared to monoculture. Consortia of Trichoderma asperellum PEC-6 and P. ostreatus increased 1.60-fold the xylanase and 1.42-fold the Beta-glucosidase productions. T. asperellum PEC-17 and A. niger increased 1.16-fold endoglucanase and 1.28-fold pectinase. Saccharification of pineapple crown waste using enzyme cocktail produced by filamentous fungi consortia increased the total reducing sugar released in 12.50–13.93% compared to enzymes produced by monocultures. This study provides an alternative model to the cocktail enzyme production by mixed cultures development with lower cost of on-site enzyme manufacture by use of agroindustrial wastes eliminating an enzyme blended step. Moreover, the procedure used in this work can be potentially cost saving and environmentally friendly and should be explored on other bioenergy feedstocks and feed production.
650 $aAgricultural wastes
650 $aAspergillus
650 $aBioenergy industry
650 $aBiomass
650 $aEnzymes
650 $aIndustrial wastes
650 $aLignocellulosic wastes
650 $aMicrobial culture
650 $aPineapples
650 $aTrichoderma asperellum
650 $aAbacaxi
650 $aBioenergia
650 $aBiomassa
650 $aEnzima
650 $aResíduo Industrial
650 $aTrichoderma
653 $aBiomass saccharification
653 $aFilamentous Fungi Isolation
653 $aMicrobial consortia
653 $aResíduos lignocelulósicos
653 $aSolid-state cultivation
700 1 $aBATISTA, R. D.
700 1 $aSANTOS, C. C. A. do A.
700 1 $aFREITAS JÚNIOR, A. C. F.
700 1 $aTERRASAN, C. R. F.
700 1 $aSANTANA, M. W. P. R. de
700 1 $aSIQUEIRA, F. G. de
700 1 $aPAULA-ELIAS, F. C. de
700 1 $aALMEIDA, A. F. de
773 $tWaste Biomass Valorization, 2020.
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