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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
02/12/2005 |
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Data da última atualização: |
21/08/2009 |
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Autoria: |
BELINTANI, P.; GASPAR, J. O.; ALMEIDA, A. M. R. |
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Título: |
Sequencing of the triple gene block of Cole latent virus. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Virus Reviews & Research, Rio de Janeiro, v. 10, p. 142, Nov. 2005. Supplement, ref. P-197. |
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Idioma: |
Inglês |
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Notas: |
Edição dos Resumos da XVI National Meeting of Virology, nov. 2005. |
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Conteúdo: |
Cole latent virus (ColLV) was first detected in Brassica oleracea var. acephala in São Paulo State, Brazil. The virus is transmitted mechanically and also inefficiently in a non-psersistent manner by Myzus persicae. Some molecular properties os CoLV has been determined (Belintani et al. J. Phytpathol. 150:330-333, 2002). and the coat protein gene sequenced (Belintani et al. GeneBank AY340584). As a consequence, recently the virus was designate as a definitive species in the genus (Carlavirus by the International Committee on Taxanomy of Viruses (Mayo. Arch. Virol. 150:189-198, 2005). Here, we describe the sequencing of the Triple Gene Block (TGB) of Cole latent virus. The RNA was extracted from purified virus particles and used to generate genomic library, making use of the Universal RiboClone cDNA Synthesis System (Promega). Several overlapping clones were generated and their nucleotide sequences were determined in both strands and analyzed by the BLAST program. Sequences of carlaviruses for comparisons were obtained from the GeneBank Database. The sequence of the TGB showed three overlapping ORFs predicting proteins of 25K, 12K and 7 K, an organization conserved among carlaviruses and potexviruses. The 25K and 12K proteins share amino acids identity of 50-66% with Hop latent virus AB032469), Potato virus M(AF023877), Aconitum latent virus (AB56118) and Potato virus S (D00461). The 7 K protein showed amino acids identity of 24-40% with the same viruses. However, the Cole latent virus coat protein gene has a higher identity with Potato virus M (Belintani et al. Fitopatol. Bras. 28:288, 2003). and probably they are closely related viruses yet distinct. Our results support the classification of CoLV as a distinct species of the Carlavirus genus. MenosCole latent virus (ColLV) was first detected in Brassica oleracea var. acephala in São Paulo State, Brazil. The virus is transmitted mechanically and also inefficiently in a non-psersistent manner by Myzus persicae. Some molecular properties os CoLV has been determined (Belintani et al. J. Phytpathol. 150:330-333, 2002). and the coat protein gene sequenced (Belintani et al. GeneBank AY340584). As a consequence, recently the virus was designate as a definitive species in the genus (Carlavirus by the International Committee on Taxanomy of Viruses (Mayo. Arch. Virol. 150:189-198, 2005). Here, we describe the sequencing of the Triple Gene Block (TGB) of Cole latent virus. The RNA was extracted from purified virus particles and used to generate genomic library, making use of the Universal RiboClone cDNA Synthesis System (Promega). Several overlapping clones were generated and their nucleotide sequences were determined in both strands and analyzed by the BLAST program. Sequences of carlaviruses for comparisons were obtained from the GeneBank Database. The sequence of the TGB showed three overlapping ORFs predicting proteins of 25K, 12K and 7 K, an organization conserved among carlaviruses and potexviruses. The 25K and 12K proteins share amino acids identity of 50-66% with Hop latent virus AB032469), Potato virus M(AF023877), Aconitum latent virus (AB56118) and Potato virus S (D00461). The 7 K protein showed amino acids identity of 24-40% with the same viruses. However, the Cole la... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 02295naa a2200157 a 4500
001 1468610
005 2009-08-21
008 2005 bl --- 0-- u #d
100 1 $aBELINTANI, P.
245 $aSequencing of the triple gene block of Cole latent virus.
260 $c2005
500 $aEdição dos Resumos da XVI National Meeting of Virology, nov. 2005.
520 $aCole latent virus (ColLV) was first detected in Brassica oleracea var. acephala in São Paulo State, Brazil. The virus is transmitted mechanically and also inefficiently in a non-psersistent manner by Myzus persicae. Some molecular properties os CoLV has been determined (Belintani et al. J. Phytpathol. 150:330-333, 2002). and the coat protein gene sequenced (Belintani et al. GeneBank AY340584). As a consequence, recently the virus was designate as a definitive species in the genus (Carlavirus by the International Committee on Taxanomy of Viruses (Mayo. Arch. Virol. 150:189-198, 2005). Here, we describe the sequencing of the Triple Gene Block (TGB) of Cole latent virus. The RNA was extracted from purified virus particles and used to generate genomic library, making use of the Universal RiboClone cDNA Synthesis System (Promega). Several overlapping clones were generated and their nucleotide sequences were determined in both strands and analyzed by the BLAST program. Sequences of carlaviruses for comparisons were obtained from the GeneBank Database. The sequence of the TGB showed three overlapping ORFs predicting proteins of 25K, 12K and 7 K, an organization conserved among carlaviruses and potexviruses. The 25K and 12K proteins share amino acids identity of 50-66% with Hop latent virus AB032469), Potato virus M(AF023877), Aconitum latent virus (AB56118) and Potato virus S (D00461). The 7 K protein showed amino acids identity of 24-40% with the same viruses. However, the Cole latent virus coat protein gene has a higher identity with Potato virus M (Belintani et al. Fitopatol. Bras. 28:288, 2003). and probably they are closely related viruses yet distinct. Our results support the classification of CoLV as a distinct species of the Carlavirus genus.
700 1 $aGASPAR, J. O.
700 1 $aALMEIDA, A. M. R.
773 $tVirus Reviews & Research, Rio de Janeiro$gv. 10, p. 142, Nov. 2005. Supplement, ref. P-197.
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