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Registros recuperados : 33 | |
2. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | ALEMÁN GAINZA, Y.; CHAPAVAL, L.; ESTELLA, B. M.; VACCARI, N. A.; CHAGAS, A. C. de S. Detection of bacterial contaminants by the larval development test. In: ENCONTRO CIENTÍFICO DE PRODUÇÃO ANIMAL SUSTENTÁVEL, 7., 2017, Campinas, SP . Anais... Nova Odessa, SP: Instituto de Zootecnia, 2017. Boletim de Indústria Animal, v. 74. Biblioteca(s): Embrapa Pecuária Sudeste. |
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3. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | ALEMÁN GAINZA, Y.; FANTATTO, R. R.; NICIURA, S. C. M.; CHAGAS, A. C. de S. Determination of parasite resistance status to anthelmintics through the larval development test. In: ENCONTRO CIENTÍFICO DE PRODUÇÃO ANIMAL SUSTENTÁVEL, 7., 2017, Campinas, SP . Anais... Nova Odessa, SP: Instituto de Zootecnia, 2017. Boletim de Indústria Animal, v. 74. Biblioteca(s): Embrapa Pecuária Sudeste. |
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7. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | NUCCI, A. DA SILVA; GIRALDELO, L. A.; SANTOS, I. B. DOS; ALEMÁN GAINZA, Y.; NICIURA, S. C. M. Padronização de co-cultivo in vitro de Haemonchus contortus com células abomasais ovinas. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 12., 2020, São Carlos, SP. Anais... São Carlos, SP: Embrapa Instrumentação; Embrapa Pecuária Sudeste, 2020. p.13. (Embrapa Instrumentação. Documentos, 71). Biblioteca(s): Embrapa Pecuária Sudeste. |
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8. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | ALEMÁN GAINZA, Y.; LOPES, L. G; SILVA, M. H da; GIRALDELO, L. A; CHAGAS, A. C. de S. Otimização do teste de desenvolvimento larvar para o monitoramento da resistência anti-helmíntica. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 20., 2018, Londrina, PR. Anais...Londrina, PR: CBPV, 2018. p. 400. Biblioteca(s): Embrapa Pecuária Sudeste. |
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9. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | ALEMÁN GAINZA, Y.; SANTOS, I. B. dos; FIGUEIREDO, A.; GIRALDELO, L. A.; CHAGAS, A. C. de S. Diagnóstico laboratorial da resistência de nematoides gastrintestinais de propriedades ovinas aos anti-helmínticos. In: CONGRESSO BRASILEIRO DE BUIATRIA, 13., 2019, Passo Fundo, RS. Anais... Passo Fundo, RS: UPF, 2019. 272 Biblioteca(s): Embrapa Pecuária Sudeste. |
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10. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | LIMA, C. S.; OLIVEIRA, M.; KETAVONG, S.; FERRAZ JUNIOR, R. S.; ALEMÁN GAINZA, Y.; CHAGAS, A. C. de S.; REGASINI, L. O.; HOSTE, H. Anthelminthic evaluation of extracts and natural compounds from Pterogyne nitens(Fabaceae) against nematodes of veterinary interest. In: JOURNÉES DU CONSORTIUM ANTI-PARASITAIRE ET ANTI-FONGIQUE, 9., 2019, Rouen, proceedings... Rouen: CaPF, 2019. Biblioteca(s): Embrapa Pecuária Sudeste. |
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11. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | ALEMÁN GAINZA, Y.; SANTOS, I. B. DOS; FIGUEIREDO, A.; SANTOS, L. A. L. DOS; ESTEVES, S. N.; BARIONI JUNIOR, W.; MINHO, A. P.; CHAGAS, A. C. de S. Anthelmintic resistance of Haemonchus contortus from sheep flocks in Brazil: concordance of in vivo and in vitro (RESISTA-Test©) methods. Revista Brasileira de Parasitologia Veterinária, v. 30, n.2, maio, 2021. 10 p. Biblioteca(s): Embrapa Pecuária Sudeste. |
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13. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | FANTATTO, Rafaela Regina; CHAGAS, A. C. de S.; ALEMÁN GAINZA, Y.; MINHO, A. P.; POLITI, F. A. S.; GREATTI, V. R.; PIETRO, R. C. L. R. Avaliação do óleo essencial de Schinus lentiscifolius frente a eclodibilidade de ovos do parasita gastrointestinal Haemonchus contortus. In: JORNADA PAULISTA DE PLANTAS MEDICINAIS "PROFESSOR DR. FRANCISCO JOSÉ DE ABREU MATOS", 13., 2017, Araraquara. Resumos... Araraquara: Unesp, 2017. p.38. Biblioteca(s): Embrapa Pecuária Sudeste. |
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14. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SILVA, M. H.; LOPES, L. G.; GIRALDELO, L. A.; ALEMÁN GAINZA, Y.; CANUTO, K. M.; CHAGAS, A. C. de S.; MIGUEL, M. C. V. Eficácia anti-helmíntica de óleos essenciais sobre o nematoide gastrintestinal Haemonchus contortus. In: JORNADA PAULISTA DE PLANTAS MEDICINAIS "PROFESSOR DR. FRANCISCO JOSÉ DE ABREU MATOS", 13., 2017, Araraquara. Resumos... Araraquara: Unesp, 2017. p.48. Biblioteca(s): Embrapa Pecuária Sudeste. |
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15. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | FIGUEIREDO, A.; COLA, D. F.; PROENÇA, P. L. F.; J. H. B. Toscano; ALEMÁN GAINZA, Y.; FRACETO, L. F. F.; CHAGAS, A. C. de S. Nanocarrier formulations with acaricide potential on Rhipicephalus (Boophilus) microplus. In: WORKSHOP ON ENVIROMENTAL NANOTECHNOLOGY, 3., 2018, Sorocaba, SP. Proceedings...Sorocaba, SP: University of Sorocaba, 2018. p. 30 Biblioteca(s): Embrapa Pecuária Sudeste. |
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16. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | ALEMAN GAINZA, Y.; LOPES, L. G.; SILVA, M. H.; GIRALDELO, L. A.; BIZZO, H. R.; GAMA, P. E.; CHAVES, F. C. M.; CHAGAS, A. C. S. Estudo comparativo da atividade anti-helmíntica de óleos essenciais de Piper sobre o nematoide parasita de ovinos, Haemonchus contortus. In: SIMPÓSIO BRASILEIRO DE ÓLEOS ESSENCIAIS, 9., 2017, Caxias do Sul, RS. Inovação e Sustentabilidade. [Caxias do Sul: UCS, 2017]. 20 a 22 nov. SBOE. e-Pôster. Ref. 68. Biblioteca(s): Embrapa Pecuária Sudeste. |
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17. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | ALEMÁN GAINZA, Y.; DOMINGUES, L. F.; PEREZ, O. P.; CHAVES, F. C. M.; BIZZO, H. R.; ESTEVES, S. N.; LOPEZ, E. R.; CHAGAS, A. C. de S. Evaluación in vitro de la actividad antihelmíntica del aceite esencial de Piper aduncum contra aislados resistente y sensíble de Haemonchus contortus. In: XXIV CONGRESO PANAMERICANO DE CIENCIAS VETERINARIAS, anais., 2014. Biblioteca(s): Embrapa Amazônia Ocidental. |
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18. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | ALEMÁN GAINZA, Y.; DOMINGUES, L. F.; PEREZ, O. P.; CHAVES, F. C. M.; BIZZO, H. R.; ESTEVES, S. N.; LOPEZ, E. R.; CHAGAS, A. C. de S. Evaluación in vitro de la actividad antihelmíntica del aceite esencial de Piper aduncum contra aislados resistente y sensíble de Haemonchus contortus. In: XXIV CONGRESO PANAMERICANO DE CIENCIAS VETERINARIAS, anais., 2014. Biblioteca(s): Embrapa Pecuária Sudeste. |
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20. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | ALEMÁN GAINZA, Y.; SANTOS, I. B. dos; FIGUEIREDO, A.; GIRALDELO, L. A.; Oliveira, I. F.; ESTEVES, S. N.; CHAGAS, A. C. de S. Comparação de métodos de análise do teste de redução da contagem de ovos nas fezes (TRCOF) para a detecção da resistência anti-helmíntica em rebanhos ovinos. In: CONGRESSO BRASILEIRO DE BUIATRIA, 13., 2019, Passo Fundo, RS. Anais... Passo Fundo, RS: UPF, 2019. 269 Biblioteca(s): Embrapa Pecuária Sudeste. |
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Registros recuperados : 33 | |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
02/12/2015 |
Data da última atualização: |
15/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
CHAGAS, A. C. de S.; DOMINGUES, L. F.; ALEMÁN GAINZA, Y.; BARIONI JUNIOR, W.; ESTEVES, S. N.; NICIURA, S. C. M. |
Afiliação: |
ANA CAROLINA DE SOUZA CHAGAS, CPPSE; LUCIANA FERREIRA DOMINGUES; Yousmel Alemán Gainza, CENTRO NACIONAL DE SANIDAD AGROPECUARIA; WALDOMIRO BARIONI JUNIOR, CPPSE; SERGIO NOVITA ESTEVES, CPPSE; SIMONE CRISTINA MEO NICIURA, CPPSE. |
Título: |
Target selected treatment with levamisole to control the development of anthelmintic resistance in a sheep flock. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Parasitology Research, v. 115, n. 3, p. 1131-1139, mar. 2016. |
DOI: |
10.1007/s00436-015-4844-x |
Idioma: |
Inglês |
Conteúdo: |
Levamisole phosphate, chosen based on its 100 % efficacy demonstrated by a previous fecal egg count reduction test (FECRT), was used as the exclusive anthelmintic treatment in the Embrapa Southeast Livestock sheep flock from 2009 to 2014 in a target selected treatment scheme. In the present study, the effectiveness of this nematode control scheme was evaluated after 5 years by FECRT, larval development test (LDT), and a molecular test to assess the development of levamisole resistance in Haemonchus contortus. Animals were submitted to treatments with albendazole, levamisole, closantel, ivermectin, moxidectin, and monepantel. Eggs per gram of feces (EPG) counts and fecal cultures were performed, and anthelmintic efficacy was calculated by the RESO 4.0 program. The helminths of the flock (GIN Embrapa2014) were compared to susceptible (McMaster) and resistant (Embrapa2010) H. contortus isolates in the LDT to estimate the LC50 and LC90 of levamisole and in a molecular test to evaluate the 63-bp indel in the acr8 gene associated with levamisole resistance. In the FECRT, parasites were susceptible to monepantel (99.6 %) and closantel (98.3 %), but resistant to moxidectin (93.8 %), levamisole (70.4 %), ivermectin (48.1 %), and albendazole (0 %). In the coproculture on D14, and the control group presented 80 % H. contortus and 20 % Trichostrongylus sp., while in the monepantel group L1 were observed as well as Oesophagostomum sp. L3. LDT and resistance factors provided good separation between susceptible and resistant parasites. The genotypic frequencies of the 63-bp insertion in the acr8 gene in H. contortus were 11.9, 6.7, and 0 % in GIN Embrapa2014, Embrapa2010, and McMaster isolates, respectively. After 5 years of exclusive use, the nematodes developed resistance to levamisole, detected by FECRT and by increase in LC50 and LC90 for levamisole in the LDT. The 63-bp indel was not confirmed as a molecular marker of levamisole resistance in our isolates. The target selected treatment scheme was effective to control helminths in the sheep flock for 5 years, when levamisole?s inefficacy was perceived because of no change in the clinical situation of treated animals. Through this scheme, it was possible to promote reversion towards susceptibility or increase of efficacy for other chemical classes. Thus, this is a valid recommendation to control worms and to delay the development of resistance, preserving other anthelmintic classes for future use. MenosLevamisole phosphate, chosen based on its 100 % efficacy demonstrated by a previous fecal egg count reduction test (FECRT), was used as the exclusive anthelmintic treatment in the Embrapa Southeast Livestock sheep flock from 2009 to 2014 in a target selected treatment scheme. In the present study, the effectiveness of this nematode control scheme was evaluated after 5 years by FECRT, larval development test (LDT), and a molecular test to assess the development of levamisole resistance in Haemonchus contortus. Animals were submitted to treatments with albendazole, levamisole, closantel, ivermectin, moxidectin, and monepantel. Eggs per gram of feces (EPG) counts and fecal cultures were performed, and anthelmintic efficacy was calculated by the RESO 4.0 program. The helminths of the flock (GIN Embrapa2014) were compared to susceptible (McMaster) and resistant (Embrapa2010) H. contortus isolates in the LDT to estimate the LC50 and LC90 of levamisole and in a molecular test to evaluate the 63-bp indel in the acr8 gene associated with levamisole resistance. In the FECRT, parasites were susceptible to monepantel (99.6 %) and closantel (98.3 %), but resistant to moxidectin (93.8 %), levamisole (70.4 %), ivermectin (48.1 %), and albendazole (0 %). In the coproculture on D14, and the control group presented 80 % H. contortus and 20 % Trichostrongylus sp., while in the monepantel group L1 were observed as well as Oesophagostomum sp. L3. LDT and resistance factors provided good separati... Mostrar Tudo |
Palavras-Chave: |
Acr8 gene; Genetic marker; Haemonchuscontortus isolates FECRT; Levamisole resistance; Reversion. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 03293naa a2200253 a 4500 001 2030316 005 2023-03-15 008 2016 bl uuuu u00u1 u #d 024 7 $a10.1007/s00436-015-4844-x$2DOI 100 1 $aCHAGAS, A. C. de S. 245 $aTarget selected treatment with levamisole to control the development of anthelmintic resistance in a sheep flock.$h[electronic resource] 260 $c2016 520 $aLevamisole phosphate, chosen based on its 100 % efficacy demonstrated by a previous fecal egg count reduction test (FECRT), was used as the exclusive anthelmintic treatment in the Embrapa Southeast Livestock sheep flock from 2009 to 2014 in a target selected treatment scheme. In the present study, the effectiveness of this nematode control scheme was evaluated after 5 years by FECRT, larval development test (LDT), and a molecular test to assess the development of levamisole resistance in Haemonchus contortus. Animals were submitted to treatments with albendazole, levamisole, closantel, ivermectin, moxidectin, and monepantel. Eggs per gram of feces (EPG) counts and fecal cultures were performed, and anthelmintic efficacy was calculated by the RESO 4.0 program. The helminths of the flock (GIN Embrapa2014) were compared to susceptible (McMaster) and resistant (Embrapa2010) H. contortus isolates in the LDT to estimate the LC50 and LC90 of levamisole and in a molecular test to evaluate the 63-bp indel in the acr8 gene associated with levamisole resistance. In the FECRT, parasites were susceptible to monepantel (99.6 %) and closantel (98.3 %), but resistant to moxidectin (93.8 %), levamisole (70.4 %), ivermectin (48.1 %), and albendazole (0 %). In the coproculture on D14, and the control group presented 80 % H. contortus and 20 % Trichostrongylus sp., while in the monepantel group L1 were observed as well as Oesophagostomum sp. L3. LDT and resistance factors provided good separation between susceptible and resistant parasites. The genotypic frequencies of the 63-bp insertion in the acr8 gene in H. contortus were 11.9, 6.7, and 0 % in GIN Embrapa2014, Embrapa2010, and McMaster isolates, respectively. After 5 years of exclusive use, the nematodes developed resistance to levamisole, detected by FECRT and by increase in LC50 and LC90 for levamisole in the LDT. The 63-bp indel was not confirmed as a molecular marker of levamisole resistance in our isolates. The target selected treatment scheme was effective to control helminths in the sheep flock for 5 years, when levamisole?s inefficacy was perceived because of no change in the clinical situation of treated animals. Through this scheme, it was possible to promote reversion towards susceptibility or increase of efficacy for other chemical classes. Thus, this is a valid recommendation to control worms and to delay the development of resistance, preserving other anthelmintic classes for future use. 653 $aAcr8 gene 653 $aGenetic marker 653 $aHaemonchuscontortus isolates FECRT 653 $aLevamisole resistance 653 $aReversion 700 1 $aDOMINGUES, L. F. 700 1 $aALEMÁN GAINZA, Y. 700 1 $aBARIONI JUNIOR, W. 700 1 $aESTEVES, S. N. 700 1 $aNICIURA, S. C. M. 773 $tParasitology Research$gv. 115, n. 3, p. 1131-1139, mar. 2016.
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