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Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
16/10/2015 |
Data da última atualização: |
16/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
VIANA, J. F. C.; CARRIJO, J.; FREITAS, C. G.; PAUL, A.; ALCARAZ, J.; LACORTE, C. C.; MIGLIOLO, L.; ANDRADE, C. A.; FALCAO, R.; SANTOS, N. C.; GONÇALVES, S.; OTERO-GONZÁLES, A. J.; KHADEMHOSSEINI, A.; DIAS, S. C.; FRANCO, O. L. |
Afiliação: |
Juliane F. C. Viana, UnB; Jéssica Carrijo, UCB; Camila G. Freitas, UCB; Arghya Paul, Massachusetts Institute of Technology, Cambridge, USA; Jarib Alcaraz, Universidade Federal de Pernambuco; CRISTIANO CASTRO LACORTE, CENARGEN; Ludovico Migliolo, UCB; César A. Andrade, Universidade Federal de Pernambuco; ROSANA FALCAO, CENARGEN; Nuno C. Santos, Universidade de Lisboa; Sónia Gonçalves, Universidade de Lisboa; Anselmo J. Otero-González, Universidad de La Habana; Ali Khademhosseini, Massachusetts Institute of Technology, Cambridge, USA; Simoni C. Dias, UCB; Octávio L. Franco, UnB. |
Título: |
Antifungal nanofibers made by controlled release of sea animal derived peptide. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Nanoscale, v. 7, p. 6238-6246, 2015. |
Idioma: |
Português |
Conteúdo: |
Candida albicans is a common human-pathogenic fungal species with the ability to cause several diseases including surface infections. Despite the clear difficulties of Candida control, antimicrobial peptides (AMPs) have emerged as an alternative strategy for fungal control. In this report, different concentrations of antifungal Cm-p1 (Cencritchis muricatus peptide 1) were electrospun into nanofibers for drug delivery. The nanofibers were characterized by mass spectrometry confirming the presence of the peptide on the scaffold. Atomic force microscopy and scanning electronic microscopy were used to measure the diameters, showing that Cm-p1 affects fiber morphology as well as the diameter and scaffold thickness. The Cm-p1 release behavior from the nanofibers demonstrated peptide release from 30 min to three days, leading to effective yeast control in the first 24 hours. Moreover, the biocompatibility of the fibers were evaluated through a MTS assay as well as ROS production by using a HUVEC model, showing that the fibers do not affect cell viability and only nanofibers containing 10% Cm-p1?PVA improved ROS generation. In addition, the secretion of pro-inflammatory cytokines IL-6 and TNF-? by the HUVECs was also slightly modified by the 10% Cm-p1?PVA nanofibers. In conclusion, the electrospinning technique applied here allowed for the manufacture of biodegradable biomimetic nanofibrous extracellular membranes with the ability to control fungal infection. |
Palavras-Chave: |
Controle de fungos; Fungos; Nanofibras. |
Thesagro: |
Cândida Albicans. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/156226/1/c5nr00767d.pdf
|
Marc: |
LEADER 02381naa a2200337 a 4500 001 2026638 005 2023-03-16 008 2015 bl uuuu u00u1 u #d 100 1 $aVIANA, J. F. C. 245 $aAntifungal nanofibers made by controlled release of sea animal derived peptide.$h[electronic resource] 260 $c2015 520 $aCandida albicans is a common human-pathogenic fungal species with the ability to cause several diseases including surface infections. Despite the clear difficulties of Candida control, antimicrobial peptides (AMPs) have emerged as an alternative strategy for fungal control. In this report, different concentrations of antifungal Cm-p1 (Cencritchis muricatus peptide 1) were electrospun into nanofibers for drug delivery. The nanofibers were characterized by mass spectrometry confirming the presence of the peptide on the scaffold. Atomic force microscopy and scanning electronic microscopy were used to measure the diameters, showing that Cm-p1 affects fiber morphology as well as the diameter and scaffold thickness. The Cm-p1 release behavior from the nanofibers demonstrated peptide release from 30 min to three days, leading to effective yeast control in the first 24 hours. Moreover, the biocompatibility of the fibers were evaluated through a MTS assay as well as ROS production by using a HUVEC model, showing that the fibers do not affect cell viability and only nanofibers containing 10% Cm-p1?PVA improved ROS generation. In addition, the secretion of pro-inflammatory cytokines IL-6 and TNF-? by the HUVECs was also slightly modified by the 10% Cm-p1?PVA nanofibers. In conclusion, the electrospinning technique applied here allowed for the manufacture of biodegradable biomimetic nanofibrous extracellular membranes with the ability to control fungal infection. 650 $aCândida Albicans 653 $aControle de fungos 653 $aFungos 653 $aNanofibras 700 1 $aCARRIJO, J. 700 1 $aFREITAS, C. G. 700 1 $aPAUL, A. 700 1 $aALCARAZ, J. 700 1 $aLACORTE, C. C. 700 1 $aMIGLIOLO, L. 700 1 $aANDRADE, C. A. 700 1 $aFALCAO, R. 700 1 $aSANTOS, N. C. 700 1 $aGONÇALVES, S. 700 1 $aOTERO-GONZÁLES, A. J. 700 1 $aKHADEMHOSSEINI, A. 700 1 $aDIAS, S. C. 700 1 $aFRANCO, O. L. 773 $tNanoscale$gv. 7, p. 6238-6246, 2015.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Meio-Norte; Embrapa Milho e Sorgo. |
Data corrente: |
23/07/2008 |
Data da última atualização: |
11/11/2016 |
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
Autoria: |
GOMIDE, R. L.; ALBUQUERQUE, P. E. P. de; VIANA, J. H. M.; ANDRADE, C. de L. T. de; GUIMARAES, C. M.; BASTOS, E. A.; DURAES, F. O. M.; MORGADO, L. B. |
Afiliação: |
REINALDO LUCIO GOMIDE, CNPMS; PAULO EMILIO PEREIRA DE ALBUQUERQUE, CNPMS; JOAO HERBERT MOREIRA VIANA, CNPMS; CAMILO DE LELIS TEIXEIRA DE ANDRADE, CNPMS; CLEBER MORAIS GUIMARAES, CNPAF; FREDERICO OZANAN MACHADO DURAES, SPM; LUIZ B. MORGADO, CPATSA. |
Título: |
Contrasting environment targets characteristics: development of the drought phenotyping network. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
In: WORKSHOP SUPPORTING EMERGENCE OR REFERENCE DROUGHT TOLERANCE PHENOTYPING CENTERS - DROUGHT PHENOTYPING NETWORK, 2008, Sete Lagoas. Proceedings... Sete Lagoas: Embrapa Milho e Sorgo, 2008. |
Páginas: |
p. 4-8. |
Idioma: |
Inglês Português |
Conteúdo: |
Introduction. Specific sites selecting criteria. Soil characteristics for establishment of a specific site. Main steps and recommendations for a specific site selection and placement. Climatic characterization. |
Thesagro: |
Clima; Resistência a Seca; Solo. |
Categoria do assunto: |
-- A Sistemas de Cultivo |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/149959/1/Contrasting-environment.pdf
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Marc: |
LEADER 01064naa a2200253 a 4500 001 2055083 005 2016-11-11 008 2008 bl uuuu u00u1 u #d 100 1 $aGOMIDE, R. L. 245 $aContrasting environment targets characteristics$bdevelopment of the drought phenotyping network. 260 $c2008 300 $ap. 4-8. 520 $aIntroduction. Specific sites selecting criteria. Soil characteristics for establishment of a specific site. Main steps and recommendations for a specific site selection and placement. Climatic characterization. 650 $aClima 650 $aResistência a Seca 650 $aSolo 700 1 $aALBUQUERQUE, P. E. P. de 700 1 $aVIANA, J. H. M. 700 1 $aANDRADE, C. de L. T. de 700 1 $aGUIMARAES, C. M. 700 1 $aBASTOS, E. A. 700 1 $aDURAES, F. O. M. 700 1 $aMORGADO, L. B. 773 $tIn: WORKSHOP SUPPORTING EMERGENCE OR REFERENCE DROUGHT TOLERANCE PHENOTYPING CENTERS - DROUGHT PHENOTYPING NETWORK, 2008, Sete Lagoas. Proceedings... Sete Lagoas: Embrapa Milho e Sorgo, 2008.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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