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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
25/06/2013 |
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Data da última atualização: |
07/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FARIA, M. T.; CARVALHO, R. F.; SEVILHANO, T. C. A.; OLIVEIRA, N. A. J.; SILVA, C. F. P.; OLIVEIRA, J. E.; SOARES, C. R. J.; GARCEZ, R.; SANTO, P. R. E.; BARTOLINI, P. |
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Afiliação: |
MARCOS TUCUNDUVA DE FARIA, CPATU; IPEN-CNEN; IPEN-CNEN; IPEN-CNEN; CPATU; IPEN-CNEN; IPEN-CNEN; USP; IPEN-CNEN; IPEN-CNEN. |
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Título: |
Isolation of the pituitary gonadotrophic a-subunit hormone of the giant amazonian fish: pirarucu (Arapaima gigas). |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Fish Physiology and Biochemistry, v. 39, n. 3, p. 683-693, Jun. 2013. |
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DOI: |
10.1007/s10695-012-9730-1 |
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Idioma: |
Inglês |
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Conteúdo: |
The cDNAs of the α-subunit of the pituitary gonadotrophic hormones (GTHα) of fish of the order Osteoglossiformes or the superorder Osteoglossomorpha have never been sequenced. For a better understanding the phylogenetic diversity and evolution of PGHα in fish and for future biotechnological synthesis of the gonadotrophic hormones (ag-FSH and ag-LH), of Arapaima gigas, one of the largest freshwater fishes of the world, its GTHα cDNA was synthesized by reverse transcriptase and the polymerase chain reaction starting from total pituitary RNA. The ag-GTHα-subunit was found to be encoded by 348 bp, corresponding to a protein of 115 amino acids, with a putative signal peptide of 24 amino acids and a mature peptide of 91 amino acids. Ten cysteine residues, responsible for forming 5 disulfide linkages, 2 putative N-linked glycosylation sites and 3 proline residues, were found to be conserved on the basis of the known sequences of vertebrate gonadotrophic hormones. Phylogenetic analysis, based on the amino acid sequences of 38 GTHα-subunits, revealed the highest identity of A. gigas with members of the Acipenseriformes, Anguilliformes, Siluriformes and Cypriniformes (87.1?89.5 %) and the lowest with Gadiformes and Cyprinodontiformes (55.0 %). The obtained phylogenetic tree agrees with previous analysis of teleostei, since A. gigas, of the order of Osteoglossiformes, appears as the sister group of Clupeocephala, while Elopomorpha forms the most basal group of all other teleosts. MenosThe cDNAs of the α-subunit of the pituitary gonadotrophic hormones (GTHα) of fish of the order Osteoglossiformes or the superorder Osteoglossomorpha have never been sequenced. For a better understanding the phylogenetic diversity and evolution of PGHα in fish and for future biotechnological synthesis of the gonadotrophic hormones (ag-FSH and ag-LH), of Arapaima gigas, one of the largest freshwater fishes of the world, its GTHα cDNA was synthesized by reverse transcriptase and the polymerase chain reaction starting from total pituitary RNA. The ag-GTHα-subunit was found to be encoded by 348 bp, corresponding to a protein of 115 amino acids, with a putative signal peptide of 24 amino acids and a mature peptide of 91 amino acids. Ten cysteine residues, responsible for forming 5 disulfide linkages, 2 putative N-linked glycosylation sites and 3 proline residues, were found to be conserved on the basis of the known sequences of vertebrate gonadotrophic hormones. Phylogenetic analysis, based on the amino acid sequences of 38 GTHα-subunits, revealed the highest identity of A. gigas with members of the Acipenseriformes, Anguilliformes, Siluriformes and Cypriniformes (87.1?89.5 %) and the lowest with Gadiformes and Cyprinodontiformes (55.0 %). The obtained phylogenetic tree agrees with previous analysis of teleostei, since A. gigas, of the order of Osteoglossiformes, appears as the sister group of Clupeocephala, while Elopomorpha forms the most basal grou... Mostrar Tudo |
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Thesagro: |
Hormônio; Reprodução. |
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Thesaurus Nal: |
Arapaima gigas. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02374naa a2200277 a 4500
001 1960558
005 2022-11-07
008 2013 bl uuuu u00u1 u #d
024 7 $a10.1007/s10695-012-9730-1$2DOI
100 1 $aFARIA, M. T.
245 $aIsolation of the pituitary gonadotrophic a-subunit hormone of the giant amazonian fish$bpirarucu (Arapaima gigas).$h[electronic resource]
260 $c2013
520 $aThe cDNAs of the α-subunit of the pituitary gonadotrophic hormones (GTHα) of fish of the order Osteoglossiformes or the superorder Osteoglossomorpha have never been sequenced. For a better understanding the phylogenetic diversity and evolution of PGHα in fish and for future biotechnological synthesis of the gonadotrophic hormones (ag-FSH and ag-LH), of Arapaima gigas, one of the largest freshwater fishes of the world, its GTHα cDNA was synthesized by reverse transcriptase and the polymerase chain reaction starting from total pituitary RNA. The ag-GTHα-subunit was found to be encoded by 348 bp, corresponding to a protein of 115 amino acids, with a putative signal peptide of 24 amino acids and a mature peptide of 91 amino acids. Ten cysteine residues, responsible for forming 5 disulfide linkages, 2 putative N-linked glycosylation sites and 3 proline residues, were found to be conserved on the basis of the known sequences of vertebrate gonadotrophic hormones. Phylogenetic analysis, based on the amino acid sequences of 38 GTHα-subunits, revealed the highest identity of A. gigas with members of the Acipenseriformes, Anguilliformes, Siluriformes and Cypriniformes (87.1?89.5 %) and the lowest with Gadiformes and Cyprinodontiformes (55.0 %). The obtained phylogenetic tree agrees with previous analysis of teleostei, since A. gigas, of the order of Osteoglossiformes, appears as the sister group of Clupeocephala, while Elopomorpha forms the most basal group of all other teleosts.
650 $aArapaima gigas
650 $aHormônio
650 $aReprodução
700 1 $aCARVALHO, R. F.
700 1 $aSEVILHANO, T. C. A.
700 1 $aOLIVEIRA, N. A. J.
700 1 $aSILVA, C. F. P.
700 1 $aOLIVEIRA, J. E.
700 1 $aSOARES, C. R. J.
700 1 $aGARCEZ, R.
700 1 $aSANTO, P. R. E.
700 1 $aBARTOLINI, P.
773 $tFish Physiology and Biochemistry$gv. 39, n. 3, p. 683-693, Jun. 2013.
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Cerrados; Embrapa Mandioca e Fruticultura; Embrapa Semiárido. |
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Data corrente: |
07/05/2019 |
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Data da última atualização: |
21/10/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
ALBUQUERQUE, H. Y. G. de; OLIVEIRA, E. J. de; BRITO, A. C.; ANDRADE, L. R. B. de; CARMO, C. D. do; MORGANTE, C. V.; VIEIRA, E. A.; CUNHA, E. F. M.; FALEIRO, F. G. |
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Afiliação: |
HILÇANA YLKA GONÇALVES DE ALBUQUERQUE, UFRB; EDER JORGE DE OLIVEIRA, CNPMF; ANA CARLA BRITO; LUCIANO ROGÉRIO BRAATZ DE ANDRADE, UFV; CÁTIA DIAS DO CARMO, UFRB; CAROLINA VIANNA MORGANTE, CPATSA; EDUARDO ALANO VIEIRA, CPAC; ELISA FERREIRA MOURA CUNHA, CPATU; FABIO GELAPE FALEIRO, CPAC. |
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Título: |
Identification of duplicates in cassava germplasm banks based on single-nucleotide polymorphisms (SNPs). |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Scientia Agricola, v. 76, n. 4, p. 328-336, jul./aug., 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
Genetic redundancy in cassava (Manihot esculenta Crantz) presents a challenge to efficient management of genetic resources. This study aimed to identify and define the ge-netic structure of duplicates in cassava germplasm from various Embrapa research units, using single-nucleotide polymorphism (SNP) markers. We evaluated 2,371 accessions with 20,712 SNPs. The identification of duplicates was performed based on multilocus genotypes (MLG), adopting a maximum genetic distance threshold of 0.05. The population structure was defined based on discriminant analysis of principal components (DAPC). A total of 1,757 unique and 614 duplicate accessions were identified. The redundancy of the collections ranged from 17% (Belém, PA/Brazil) to 39% (Petrolina, PE/Brazil), with an average of 21%. This redundancy between different research units is probably due to the historical sharing of accessions, as well as collections carried out in the same region, or even to the intense germplasm exchange between farmers with different genotype names. In terms of genetic structure, the 250 principal components explained 88 % of the genetic variation of the SNP markers and defined the hierar-chical structure of the duplicate cassava germplasm in 12 groups. Since heterotic groups have not yet been identified for cassava, crosses between accessions of the 12 DAPC groups may be promising. All MLGs were allocated within the same DAPC group, corroborating duplicate analyses yet still revealing high variability between groups that were quite distinct based on the first two discriminant functions. Our results contribute to optimizing the conservation of genetic resources, together with understanding diversity and its use in crop improvement. MenosGenetic redundancy in cassava (Manihot esculenta Crantz) presents a challenge to efficient management of genetic resources. This study aimed to identify and define the ge-netic structure of duplicates in cassava germplasm from various Embrapa research units, using single-nucleotide polymorphism (SNP) markers. We evaluated 2,371 accessions with 20,712 SNPs. The identification of duplicates was performed based on multilocus genotypes (MLG), adopting a maximum genetic distance threshold of 0.05. The population structure was defined based on discriminant analysis of principal components (DAPC). A total of 1,757 unique and 614 duplicate accessions were identified. The redundancy of the collections ranged from 17% (Belém, PA/Brazil) to 39% (Petrolina, PE/Brazil), with an average of 21%. This redundancy between different research units is probably due to the historical sharing of accessions, as well as collections carried out in the same region, or even to the intense germplasm exchange between farmers with different genotype names. In terms of genetic structure, the 250 principal components explained 88 % of the genetic variation of the SNP markers and defined the hierar-chical structure of the duplicate cassava germplasm in 12 groups. Since heterotic groups have not yet been identified for cassava, crosses between accessions of the 12 DAPC groups may be promising. All MLGs were allocated within the same DAPC group, corroborating duplicate analyses yet still revealing high variabi... Mostrar Tudo |
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Palavras-Chave: |
Cultivo. |
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Thesagro: |
Mandioca; Manihot Esculenta; Melhoramento Vegetal. |
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Thesaurus NAL: |
Breeding; Breeding and Genetic Improvement; Cassava. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/199859/1/Indetification-2019.pdf
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Marc: |
LEADER 02636naa a2200301 a 4500
001 2110860
005 2020-10-21
008 2019 bl uuuu u00u1 u #d
100 1 $aALBUQUERQUE, H. Y. G. de
245 $aIdentification of duplicates in cassava germplasm banks based on single-nucleotide polymorphisms (SNPs).$h[electronic resource]
260 $c2019
520 $aGenetic redundancy in cassava (Manihot esculenta Crantz) presents a challenge to efficient management of genetic resources. This study aimed to identify and define the ge-netic structure of duplicates in cassava germplasm from various Embrapa research units, using single-nucleotide polymorphism (SNP) markers. We evaluated 2,371 accessions with 20,712 SNPs. The identification of duplicates was performed based on multilocus genotypes (MLG), adopting a maximum genetic distance threshold of 0.05. The population structure was defined based on discriminant analysis of principal components (DAPC). A total of 1,757 unique and 614 duplicate accessions were identified. The redundancy of the collections ranged from 17% (Belém, PA/Brazil) to 39% (Petrolina, PE/Brazil), with an average of 21%. This redundancy between different research units is probably due to the historical sharing of accessions, as well as collections carried out in the same region, or even to the intense germplasm exchange between farmers with different genotype names. In terms of genetic structure, the 250 principal components explained 88 % of the genetic variation of the SNP markers and defined the hierar-chical structure of the duplicate cassava germplasm in 12 groups. Since heterotic groups have not yet been identified for cassava, crosses between accessions of the 12 DAPC groups may be promising. All MLGs were allocated within the same DAPC group, corroborating duplicate analyses yet still revealing high variability between groups that were quite distinct based on the first two discriminant functions. Our results contribute to optimizing the conservation of genetic resources, together with understanding diversity and its use in crop improvement.
650 $aBreeding
650 $aBreeding and Genetic Improvement
650 $aCassava
650 $aMandioca
650 $aManihot Esculenta
650 $aMelhoramento Vegetal
653 $aCultivo
700 1 $aOLIVEIRA, E. J. de
700 1 $aBRITO, A. C.
700 1 $aANDRADE, L. R. B. de
700 1 $aCARMO, C. D. do
700 1 $aMORGANTE, C. V.
700 1 $aVIEIRA, E. A.
700 1 $aCUNHA, E. F. M.
700 1 $aFALEIRO, F. G.
773 $tScientia Agricola$gv. 76, n. 4, p. 328-336, jul./aug., 2019.
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Embrapa Semiárido (CPATSA) |
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