Data/hora: 10/06/2024 11:30:34

Biblioteca(s): 	Embrapa Pesca e Aquicultura.	
Data corrente: 	27/10/2017	
Data da última atualização: 	27/10/2017	
Autoria: 	TORATI, L. S.; MIGAUD, H.; DOHERTY, M. K.; SIWY, J.; MULLEN, W.; MESQUITA, P. E. C.; ALBALAT, A.	
Afiliação: 	LUCAS SIMON TORATI, CNPASA; HERVE MIGAUD, UNIVERSITY OF STIRLING, UK; MARY K. DOHERTY, UNIVERSITY OF THE HIGHLANDS AND ISLANDS, UK; JUSTYNA SIWY, MOSAIQUES DIAGNOSTICS GmbH, Hannover-Germany; WILLIAN MULLEN, UNIVERSITY OF GLASGOW, UK; PEDRO E. C. MESQUITA, DNOCS; AMAYA ALBALAT, UNIVERSITY OF STIRLING, UK.	
Título: 	Comparative proteome and peptidome analysis of the cephalic fluid secreted by Arapaima gigas (Teleostei: Osteoglossidae) during and outside parental care.	
Ano de publicação: 	2017	
Fonte/Imprenta: 	Plos One, v. 12, n. 10, e0186692, Oct. 2017.	
ISSN: 	1932-6203	
DOI: 	10.1371/journal.pone.0186692	
Idioma: 	Inglês	
Conteúdo: 	Parental investment in Arapaima gigas includes nest building and guarding, followed by a care provision when a cephalic fluid is released from the parents' head to the offspring. This fluid has presumably important functions for the offspring but so far its composition has not been characterised. In this study the proteome and peptidome of the cephalic secretion was studied in parental and non-parental fish using capillary electrophoresis coupled to mass spectrometry (CE-MS) and GeLC-MS/MS analyses. Multiple comparisons revealed 28 peptides were significantly different between males and parental males (PC-males), 126 between females and parental females (PC-females), 51 between males and females and 9 between PC-males and PC-females. Identification revealed peptides were produced in the inner ear (pcdh15b), eyes (tetraspanin and ppp2r3a), central nervous system (otud4, ribeye a, tjp1b and syn1) among others. A total of 422 proteins were also identified and gene ontology analysis revealed 28 secreted extracellular proteins. From these, 2 hormones (prolactin and stanniocalcin) and 12 proteins associated to immunological processes (serotransferrin, &#945;-1-antitrypsin homolog, apolipoprotein A-I, and others) were identified. This study provides novel biochemical data on the lateral line fluid which will enable future hypotheses-driven experiments to better understand the physiological roles of the lateral line in chemical communication.	
Palavras-Chave: 	Peptidome.	
Thesagro: 	Peixe; Pirarucu.	
Thesaurus Nal: 	Alloparental behavior; Arapaima gigas; Proteome.	
Categoria do assunto: 	K Ciência Florestal e Produtos de Origem Vegetal	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/165650/1/CNPASA-2017-plos.pdf	
Registro original: 	Embrapa Pesca e Aquicultura (CNPASA)	
Exemplares: 	CNPASA - (553-1) - UPC - AP - DD - CNPASA_AP48 -  - 2017.048 - DIS