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Registro Completo |
Biblioteca(s): |
Embrapa Agroenergia. |
Data corrente: |
29/09/2015 |
Data da última atualização: |
20/09/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SANTOS, D. F. K. dos; ISTVAN, P.; NORONHA, E. F.; QUIRINO, B. F.; KRÜGER, R. H. |
Afiliação: |
Débora Farage Knupp dos Santos, UnB; Paula Istvan, UnB; Eliane Ferreira Noronha, UnB; BETANIA FERRAZ QUIRINO, CNPAE; Ricardo Henrique Krüger, UnB. |
Título: |
New dioxygenase from metagenomic library from Brazilian soil: insights into antibiotic resistance and bioremediation. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Biotechnology Letters, v. 37, n. 9, p.1809-1817, 2015. |
DOI: |
10.1007/s10529-015-1861-x |
Idioma: |
Inglês |
Conteúdo: |
Objectives Putative new dioxygenases were identified in a metagenomic b-lactam-resistance screening and, given their key role on aromatic metabolism, we raise the hypothesis that these enzymes maybe concomitantly related to antibiotic resistance and aromatic degradation. Results ORFs of three putative dioxygenases were isolated from resistant metagenomic clones. One of them, CRB2(1), was subcloned into pET24a expression vector and subjected to downstream phenotypic and bioinformatics analyses that demonstrated the ??dual effect?? of our metagenomic dioxygenase, on antibiotic and aromatic resistance. Furthermore, initial characterization assays strongly suggests that CRB2(1) open-reading frame is an extradiol-dioxygenase, most probably a bicupin domain gentisate 1,2-dioxygenase. This observation is, to our knowledge, the first description of a metagenomic dioxygenase and its action on b-lactam resistance. Conclusion Unraveling the diversity of antibiotic resistance elements on the environment could not only identify new genes and mechanisms in which bacteria can resist to antibiotics, but also contribute to biotechnology processes, such as in bioremediation. |
Palavras-Chave: |
Aromatic metabolism; Aromatic metabolismo; Dioxygenase; Dioxygenase Metagenome; Metagenome; Resistência aos antibióticos. |
Thesagro: |
Solo. |
Thesaurus Nal: |
antibiotic resistance; soil. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02061naa a2200289 a 4500 001 2025343 005 2017-09-20 008 2015 bl uuuu u00u1 u #d 024 7 $a10.1007/s10529-015-1861-x$2DOI 100 1 $aSANTOS, D. F. K. dos 245 $aNew dioxygenase from metagenomic library from Brazilian soil$binsights into antibiotic resistance and bioremediation.$h[electronic resource] 260 $c2015 520 $aObjectives Putative new dioxygenases were identified in a metagenomic b-lactam-resistance screening and, given their key role on aromatic metabolism, we raise the hypothesis that these enzymes maybe concomitantly related to antibiotic resistance and aromatic degradation. Results ORFs of three putative dioxygenases were isolated from resistant metagenomic clones. One of them, CRB2(1), was subcloned into pET24a expression vector and subjected to downstream phenotypic and bioinformatics analyses that demonstrated the ??dual effect?? of our metagenomic dioxygenase, on antibiotic and aromatic resistance. Furthermore, initial characterization assays strongly suggests that CRB2(1) open-reading frame is an extradiol-dioxygenase, most probably a bicupin domain gentisate 1,2-dioxygenase. This observation is, to our knowledge, the first description of a metagenomic dioxygenase and its action on b-lactam resistance. Conclusion Unraveling the diversity of antibiotic resistance elements on the environment could not only identify new genes and mechanisms in which bacteria can resist to antibiotics, but also contribute to biotechnology processes, such as in bioremediation. 650 $aantibiotic resistance 650 $asoil 650 $aSolo 653 $aAromatic metabolism 653 $aAromatic metabolismo 653 $aDioxygenase 653 $aDioxygenase Metagenome 653 $aMetagenome 653 $aResistência aos antibióticos 700 1 $aISTVAN, P. 700 1 $aNORONHA, E. F. 700 1 $aQUIRINO, B. F. 700 1 $aKRÜGER, R. H. 773 $tBiotechnology Letters$gv. 37, n. 9, p.1809-1817, 2015.
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Embrapa Agroenergia (CNPAE) |
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Registro Completo
Biblioteca(s): |
Embrapa Instrumentação. |
Data corrente: |
05/02/2010 |
Data da última atualização: |
19/04/2010 |
Autoria: |
CORRADINI, E.; IMAM, S. H.; AGNELLI, J. A. M.; MATTOSO, L. H. C. |
Afiliação: |
LUIZ HENRIQUE CAPPARELLI MATTOSO, CNPDIA. |
Título: |
Effect of coconut, sisal and jute fibers on the properties of starch/gluten/glycerol matrix. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Journal of polymers and the environment, [S .l.], v. 17, n. 1, p. 1-9, 2009. |
Idioma: |
Inglês |
Palavras-Chave: |
Coconut. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00465naa a2200133 a 4500 001 1656981 005 2010-04-19 008 2009 bl --- 0-- u #d 100 1 $aCORRADINI, E.; IMAM, S. H.; AGNELLI, J. A. M. 245 $aEffect of coconut, sisal and jute fibers on the properties of starch/gluten/glycerol matrix. 260 $c2009 653 $aCoconut 700 1 $aMATTOSO, L. H. C. 773 $tJournal of polymers and the environment, [S .l.]$gv. 17, n. 1, p. 1-9, 2009.
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