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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
07/05/2020 |
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Data da última atualização: |
15/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUSA, A. M. P.; SALLES, H. O.; OLIVEIRA, H. D. de; SOUZA, B. B. P. de; CARDOZO FILHO, J. de L.; SIFUENTES, D. N.; PRATES, M. V.; BLOCH JUNIOR, C.; BEMQUERER, M. P.; VASCONCELOS, A. S. do E. |
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Afiliação: |
ANA MARJORY PAIVA SOUSA, Rede Nordeste de Biotecnologia-RENORBIO, Universidade Estadual do Ceará (UECe) - Fortaleza, CE, Brazil; HEVILA OLIVEIRA SALLES, CNPC; HERMOGENES DAVID DE OLIVEIRA, Universidade Federal do Ceará (UFC) - Fortaleza, CE, Brazil; BEATRIZ BLENDA PINHEIRO DE SOUZA; JOSE DE LIMA CARDOZO FILHO; DANIEL NOGOCEKE SIFUENTES, Cenargen; MAURA VIANNA PRATES, Cenargen; CARLOS BLOCH JUNIOR, Cenargen; MARCELO PORTO BEMQUERER, Cenargen; ANTONIO SILVIO DO EGITO VASCONCELOS, CNPC. |
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Título: |
Mo-HLPs: new flocculating agents identified from Moringa oleifera seeds belong to the hevein-like peptide family. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Journal of Proteomics, v. 217, article 103692, Apr. 2020. |
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DOI: |
https://doi.org/10.1016/j.jprot.2020.103692 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Cationic peptides found in Moringa oleifera seeds belong to different protein families and are described as the main flocculating agents of the species. In this study we report the identification and isolation of four new flocculant peptides, called Mo-HLPs 1?4, belonging to the family of hevein-like peptides, previously only known for their members' antimicrobial activity. Purification of the peptides followed two sequential membrane ultrafiltration steps and separation by reverse-phase liquid chromatography. Proteomic analyses showed that Mo-HLPs are extremely basic (pI >10) cysteine-rich molecules with molecular masses between 4.5 and 4.8 kDa and with a highly conserved chitin-binding domain. Searches in BLAST revealed high similarity of Mo-HLPs with hevein and other hevein-like peptides and 90% identity with morintides, which are members of the 8C-hevein-like subfamily found in M. oleifera leaves. Mo-HLPs microflocculation assays showed distinct coagulation/flocculation efficiencies, promoting turbidity reduction levels between 67 and 89% in synthetic turbid water. Activity variations were attributed to the substitution of some amino acids among the isoforms, which may have altered the final net charge of the molecules. The identification of Mo-HLPs represents the discovery of a new group of cationic peptides involved in the flocculation properties of M. oleifera seeds. |
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Palavras-Chave: |
Cationic peptides; Flocculant activity; Hevein-like. |
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Thesagro: |
Moringa Oleifera. |
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Categoria do assunto: |
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Marc: |
LEADER 02333naa a2200289 a 4500
001 2122135
005 2020-12-15
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.jprot.2020.103692$2DOI
100 1 $aSOUSA, A. M. P.
245 $aMo-HLPs$bnew flocculating agents identified from Moringa oleifera seeds belong to the hevein-like peptide family.$h[electronic resource]
260 $c2020
520 $aAbstract: Cationic peptides found in Moringa oleifera seeds belong to different protein families and are described as the main flocculating agents of the species. In this study we report the identification and isolation of four new flocculant peptides, called Mo-HLPs 1?4, belonging to the family of hevein-like peptides, previously only known for their members' antimicrobial activity. Purification of the peptides followed two sequential membrane ultrafiltration steps and separation by reverse-phase liquid chromatography. Proteomic analyses showed that Mo-HLPs are extremely basic (pI >10) cysteine-rich molecules with molecular masses between 4.5 and 4.8 kDa and with a highly conserved chitin-binding domain. Searches in BLAST revealed high similarity of Mo-HLPs with hevein and other hevein-like peptides and 90% identity with morintides, which are members of the 8C-hevein-like subfamily found in M. oleifera leaves. Mo-HLPs microflocculation assays showed distinct coagulation/flocculation efficiencies, promoting turbidity reduction levels between 67 and 89% in synthetic turbid water. Activity variations were attributed to the substitution of some amino acids among the isoforms, which may have altered the final net charge of the molecules. The identification of Mo-HLPs represents the discovery of a new group of cationic peptides involved in the flocculation properties of M. oleifera seeds.
650 $aMoringa Oleifera
653 $aCationic peptides
653 $aFlocculant activity
653 $aHevein-like
700 1 $aSALLES, H. O.
700 1 $aOLIVEIRA, H. D. de
700 1 $aSOUZA, B. B. P. de
700 1 $aCARDOZO FILHO, J. de L.
700 1 $aSIFUENTES, D. N.
700 1 $aPRATES, M. V.
700 1 $aBLOCH JUNIOR, C.
700 1 $aBEMQUERER, M. P.
700 1 $aVASCONCELOS, A. S. do E.
773 $tJournal of Proteomics$gv. 217, article 103692, Apr. 2020.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
06/04/2004 |
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Data da última atualização: |
27/07/2007 |
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Autoria: |
SOUZA, P. I. M.; MOREIRA, C. T.; FARIAS NETO, A. L.; ALMEIDA, L. A. de; KIIHL, R. A. de S.; FARIA, L. C.; ARANTES, N. E.; SPEHAR, C. R.; ABUD, S.; NUNES JÚNIOR, J. |
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Título: |
BRS Petala soybean cultivar. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. |
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Páginas: |
p. 174-175. |
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Série: |
(Embrapa Soja. Documentos, 228). |
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Idioma: |
Inglês |
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Notas: |
Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. |
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Conteúdo: |
BRS Petala soybean cultivar was developed by a modified pedigree selection from the cross Bragg X [Braxton*2 X (BR 27*4 X Cristalina)]. It was extensively evaluated under the experimental designation BR95-1985. It is recommended for planting in the states of Goias, Bahia, Minas Gerais, Mato Grosso, Tocantins and Distrito Federal. Petala has determinate growth habit and reaches maturity approximately 139 days after emergence. It has purple flower, tawny pubescence, seeds with black hila and 100-seed weight of 17 grams. Petala has good resistance to both lodging and shattering. Average plant height is 85 cm and is adapted for planting in different regions and planting dates. Petala is resistant to frogeye leaf spot (Cercospora sojina), bacterial pustule (Pseudomonas syringae pv. glycinea) and moderately resistant to SDS (Fusarium solani f.sp. Glycines) and stem canker (Diaphorte phaseolorum). It has also good resistance to root-knot nematode Meloydogine javanica and moderate resistance to root-knot nematode Meloydogine incognita. In performance trials across 27 locations at cerrado region, over 2 years Petala had higher seed yield than the checks Uirapuru, 'FT 104' 'DM 339', 'M-SOY 8800' and 'Garça Branca'. The average seed yield of Petala was 3381 kg/ha in Tocantins, 3069 kg/ha in Minas Gerais, 3447 kg/ha and Mato Grosso and has a yield potential of 4085 kg/ha. It exibits a high stability of seed yield and is best adapted when planted in November at plant populations of 250,000 to 300,000 plants/ha in Minas Gerais, 300,000 to 350,000 plants/ha in Goias and Distrito Federal and 300,000 plants/ha in Tocantins and Mato Grosso. Petala is a high yielding, root-knot nematode resistant soybean cultivar available for soybean farmers. MenosBRS Petala soybean cultivar was developed by a modified pedigree selection from the cross Bragg X [Braxton*2 X (BR 27*4 X Cristalina)]. It was extensively evaluated under the experimental designation BR95-1985. It is recommended for planting in the states of Goias, Bahia, Minas Gerais, Mato Grosso, Tocantins and Distrito Federal. Petala has determinate growth habit and reaches maturity approximately 139 days after emergence. It has purple flower, tawny pubescence, seeds with black hila and 100-seed weight of 17 grams. Petala has good resistance to both lodging and shattering. Average plant height is 85 cm and is adapted for planting in different regions and planting dates. Petala is resistant to frogeye leaf spot (Cercospora sojina), bacterial pustule (Pseudomonas syringae pv. glycinea) and moderately resistant to SDS (Fusarium solani f.sp. Glycines) and stem canker (Diaphorte phaseolorum). It has also good resistance to root-knot nematode Meloydogine javanica and moderate resistance to root-knot nematode Meloydogine incognita. In performance trials across 27 locations at cerrado region, over 2 years Petala had higher seed yield than the checks Uirapuru, 'FT 104' 'DM 339', 'M-SOY 8800' and 'Garça Branca'. The average seed yield of Petala was 3381 kg/ha in Tocantins, 3069 kg/ha in Minas Gerais, 3447 kg/ha and Mato Grosso and has a yield potential of 4085 kg/ha. It exibits a high stability of seed yield and is best adapted when planted in November at plant populations of 250,0... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 02803naa a2200265 a 4500
001 1466791
005 2007-07-27
008 2004 bl uuuu u00u1 u #d
100 1 $aSOUZA, P. I. M.
245 $aBRS Petala soybean cultivar.
260 $c2004
300 $ap. 174-175.
490 $a(Embrapa Soja. Documentos, 228).
500 $aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.
520 $aBRS Petala soybean cultivar was developed by a modified pedigree selection from the cross Bragg X [Braxton*2 X (BR 27*4 X Cristalina)]. It was extensively evaluated under the experimental designation BR95-1985. It is recommended for planting in the states of Goias, Bahia, Minas Gerais, Mato Grosso, Tocantins and Distrito Federal. Petala has determinate growth habit and reaches maturity approximately 139 days after emergence. It has purple flower, tawny pubescence, seeds with black hila and 100-seed weight of 17 grams. Petala has good resistance to both lodging and shattering. Average plant height is 85 cm and is adapted for planting in different regions and planting dates. Petala is resistant to frogeye leaf spot (Cercospora sojina), bacterial pustule (Pseudomonas syringae pv. glycinea) and moderately resistant to SDS (Fusarium solani f.sp. Glycines) and stem canker (Diaphorte phaseolorum). It has also good resistance to root-knot nematode Meloydogine javanica and moderate resistance to root-knot nematode Meloydogine incognita. In performance trials across 27 locations at cerrado region, over 2 years Petala had higher seed yield than the checks Uirapuru, 'FT 104' 'DM 339', 'M-SOY 8800' and 'Garça Branca'. The average seed yield of Petala was 3381 kg/ha in Tocantins, 3069 kg/ha in Minas Gerais, 3447 kg/ha and Mato Grosso and has a yield potential of 4085 kg/ha. It exibits a high stability of seed yield and is best adapted when planted in November at plant populations of 250,000 to 300,000 plants/ha in Minas Gerais, 300,000 to 350,000 plants/ha in Goias and Distrito Federal and 300,000 plants/ha in Tocantins and Mato Grosso. Petala is a high yielding, root-knot nematode resistant soybean cultivar available for soybean farmers.
700 1 $aMOREIRA, C. T.
700 1 $aFARIAS NETO, A. L.
700 1 $aALMEIDA, L. A. de
700 1 $aKIIHL, R. A. de S.
700 1 $aFARIA, L. C.
700 1 $aARANTES, N. E.
700 1 $aSPEHAR, C. R.
700 1 $aABUD, S.
700 1 $aNUNES JÚNIOR, J.
773 $tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.
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