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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
02/02/2017 |
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Data da última atualização: |
30/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GOLO, P. S.; SANTOS, A. S. de O. dos; MONTEIRO, C. M. O.; PERINOTTO, W. M. de S.; QUINELATO, S.; CAMARGO, M. G.; SÁ, F. A. de; ANGELO, I. da C.; MARTINS, M. F.; PRATA, M. C. de A.; BITTENCOURT, V. R. E. P. |
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Afiliação: |
Patrícia Silva Golo, UFRRJ; Alessa Siqueira de Oliveira dos Santos; Caio Marcio Oliveira Monteiro, UFRRJ; Wendell Marcelo de Souza Perinotto, Universidade de Cuiabá; Simone Quinelato, Fundação Osvaldo Cruz; Mariana Guedes Camargo, UFRRJ; Fillipe Araujo de Sá, UFRJ; Isabele da Costa Angelo, UFRRJ; MARTA FONSECA MARTINS, CNPGL; MARCIA CRISTINA DE AZEVEDO PRATA, CNPGL; Vânia Rita Elias Pinheiro Bittencourt, UFRRJ. |
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Título: |
Lab-on-a-chip and SDS-PAGE analysis of hemolymph protein profile from Rhipicephalus microplus (Acari: Ixodidae) infected with entomopathogenic nematode and fungus. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Parasitology Research, n. 115, p. 3459-3468, 2016. |
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Idioma: |
Português |
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Conteúdo: |
Abstract In the present study, lab-on-a-chip electrophoresis (LoaC) was suggested as an alternative method to the conventional polyacrylamide gel electrophoresis under denaturing conditions (SDS-PAGE) to analyze raw cell-free tick hemolymph. Rhipicephalus microplus females were exposed to the entomopathogenic fungus Metarhizium anisopliae senso latu IBCB 116 strain and/or to the entomopathogenic nematode Heterorhabditis indica LPP1 strain. Hemolymph from not exposed or exposed ticks was collected 16 and 24 h after exposure and analyze by SDS-PAGE or LoaC. SDS-PAGE yielded 15 bands and LoaC electrophoresis 17 bands. Despite the differences in the number of bands, when the hemolymph protein profiles of exposed or unexposed ticks were compared in the same method, no suppressing or additional bandswere detected among the treatments regardless themethod (i.e., SDS-PAGE or chip electrophoresis using the Protein 230 Kit®). The potential of LoaC electrophoresis to detect protein bands from tick hemolymph was considered more efficient in comparison to the detection obtained using the traditional SDS-PAGE method, especially when it comes to protein subunits heavier than 100 KDa. LoaC electrophoresis provided a very good reproducibility, and is much faster than the conventional SDS-PAGE method, which requires several hours for one analysis. Despite both methods can be used to analyze tick hemolymph composition, LoaC was consideredmore suitable for cell-free hemolymph protein separation and detection. LoaC hemolymph band percent data reported changes in key proteins (i.e., HeLp and vitellogenin) exceptionally important for tick embryogenesis. This study reported, for the first time, tick hemolymph protein profile using LoaC. MenosAbstract In the present study, lab-on-a-chip electrophoresis (LoaC) was suggested as an alternative method to the conventional polyacrylamide gel electrophoresis under denaturing conditions (SDS-PAGE) to analyze raw cell-free tick hemolymph. Rhipicephalus microplus females were exposed to the entomopathogenic fungus Metarhizium anisopliae senso latu IBCB 116 strain and/or to the entomopathogenic nematode Heterorhabditis indica LPP1 strain. Hemolymph from not exposed or exposed ticks was collected 16 and 24 h after exposure and analyze by SDS-PAGE or LoaC. SDS-PAGE yielded 15 bands and LoaC electrophoresis 17 bands. Despite the differences in the number of bands, when the hemolymph protein profiles of exposed or unexposed ticks were compared in the same method, no suppressing or additional bandswere detected among the treatments regardless themethod (i.e., SDS-PAGE or chip electrophoresis using the Protein 230 Kit®). The potential of LoaC electrophoresis to detect protein bands from tick hemolymph was considered more efficient in comparison to the detection obtained using the traditional SDS-PAGE method, especially when it comes to protein subunits heavier than 100 KDa. LoaC electrophoresis provided a very good reproducibility, and is much faster than the conventional SDS-PAGE method, which requires several hours for one analysis. Despite both methods can be used to analyze tick hemolymph composition, LoaC was consideredmore suitable for cell-free hemolymph protein separation... Mostrar Tudo |
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Palavras-Chave: |
Cattle tick; Protein bands. |
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Thesaurus Nal: |
biological control; gel electrophoresis; Heterorhabditis; Metarhizium. |
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Categoria do assunto: |
O Insetos e Entomologia |
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Marc: |
LEADER 02735naa a2200313 a 4500
001 2062525
005 2023-01-30
008 2016 bl uuuu u00u1 u #d
100 1 $aGOLO, P. S.
245 $aLab-on-a-chip and SDS-PAGE analysis of hemolymph protein profile from Rhipicephalus microplus (Acari$bIxodidae) infected with entomopathogenic nematode and fungus.$h[electronic resource]
260 $c2016
520 $aAbstract In the present study, lab-on-a-chip electrophoresis (LoaC) was suggested as an alternative method to the conventional polyacrylamide gel electrophoresis under denaturing conditions (SDS-PAGE) to analyze raw cell-free tick hemolymph. Rhipicephalus microplus females were exposed to the entomopathogenic fungus Metarhizium anisopliae senso latu IBCB 116 strain and/or to the entomopathogenic nematode Heterorhabditis indica LPP1 strain. Hemolymph from not exposed or exposed ticks was collected 16 and 24 h after exposure and analyze by SDS-PAGE or LoaC. SDS-PAGE yielded 15 bands and LoaC electrophoresis 17 bands. Despite the differences in the number of bands, when the hemolymph protein profiles of exposed or unexposed ticks were compared in the same method, no suppressing or additional bandswere detected among the treatments regardless themethod (i.e., SDS-PAGE or chip electrophoresis using the Protein 230 Kit®). The potential of LoaC electrophoresis to detect protein bands from tick hemolymph was considered more efficient in comparison to the detection obtained using the traditional SDS-PAGE method, especially when it comes to protein subunits heavier than 100 KDa. LoaC electrophoresis provided a very good reproducibility, and is much faster than the conventional SDS-PAGE method, which requires several hours for one analysis. Despite both methods can be used to analyze tick hemolymph composition, LoaC was consideredmore suitable for cell-free hemolymph protein separation and detection. LoaC hemolymph band percent data reported changes in key proteins (i.e., HeLp and vitellogenin) exceptionally important for tick embryogenesis. This study reported, for the first time, tick hemolymph protein profile using LoaC.
650 $abiological control
650 $agel electrophoresis
650 $aHeterorhabditis
650 $aMetarhizium
653 $aCattle tick
653 $aProtein bands
700 1 $aSANTOS, A. S. de O. dos
700 1 $aMONTEIRO, C. M. O.
700 1 $aPERINOTTO, W. M. de S.
700 1 $aQUINELATO, S.
700 1 $aCAMARGO, M. G.
700 1 $aSÁ, F. A. de
700 1 $aANGELO, I. da C.
700 1 $aMARTINS, M. F.
700 1 $aPRATA, M. C. de A.
700 1 $aBITTENCOURT, V. R. E. P.
773 $tParasitology Research$gn. 115, p. 3459-3468, 2016.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 8 | |
| 1. |  | SCALCO, M. S.; ALVARENGA, L. A.; GUIMARÃES, R. J.; DOHETTI, A. W.; COLOMBO, A.; ASSIS, G. A.; ABREU, G. F. Teores foliares de fósforo e zinco, produtividade e crescimento de café irrigado. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 49, n. 2, p. 95-101, fev. 2014. Título em inglês: Leaf contents of phosphorus and zinc, productivity, and growth of irrigated coffee.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 2. | | ABREU, G. F. de; PEREIRA, C. C.; MALTA, M. R.; CLEMENTE, A. da C. S.; COELHO, L. F. S.; ROSA, S. D. V. F. da. Alterações na coloração de grãos de café em função das operações pós-colheita. Coffee Science, Lavras, v.10, n.4, p.429-436, out./dez. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Café. |
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| 3. | | ABREU, G. F. de; ROSA, S. D. V. F. da; MALTA, M. R.; CLEMENTE, A. da C. S.; VILELA, A. L.; PEREIRA, R. W. Antioxidant enzymes preserving coffee quality in refrigerate environment. Biotecnología Vegetal, v. 18, n. 3, p. 151-159, julio-septiembre, 2018 Título em espanhol: Enzimas antioxidantes preservan la calidad del café en ambiente refrigerado.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Café. |
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| 4. | | ABREU, G. F.; ROSA, S. D. V. F. da; COELHO, S. V. B.; PEREIRA, C. C.; MALTA, M. R.; FANTAZZINI, T. B.; VILELA, A. L. Influence of hulling and storage conditions on maintaining coffee quality. Anais da Academia Brasileira de Ciências, v. 95, n. 4, 2023. 12 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Café. |
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| 5. | | ABREU, G. F. de; ROSA, S. D. V. F. da; CAIXETA, F.; CLEMENTE, A. da C. S.; PEREIRA, C. C.; COELHO, S. V. B. Colorimetria em grãos de café submetidos a diferentes métodos de processamento e armazenados em ambiente resfriado. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 8., 2013, Salvador. Sustentabilidade e inclusão Social. Brasília, DF: Embrapa Café, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 6. | | MALTA, M. R.; ALCÂNTARA, E. N.; ROSA, S. D. V. F. da; ABREU, G. F.; LISKA, G. R.; FASSIO, L. de O.; LIMA, P. M. Changes in chemical composition and quality of coffee in function of different post-harvest operations. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 26., 2016, Kunming,Yunnan, China. Proceedings... ASIC - Association for Science and Information on Coffee, 2016. p. 195.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 7. | | ABREU, G. F. de; ROSA, S. D. V. F. da; CIRILLO, M. A.; MALTA, M. R.; CLEMENTE, A. C. S.; BORÉM, F. M. Simultaneous optimization of coffee quality variables during storage. Revista Brasileira de Engenharia Agrícola e Ambiental, Campo Grande, MS, v. 21, n. 1, p. 56-60, 2017| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Café. |
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| 8. | | FERREIRA, L. F.; ABREU, G. F. de; LAGO, A. M. T.; FIGUEIREDO, L. P.; BORÉM, F. M.; MARTINS, M. A.; BORGES, S. V.; DIAS, M. V. Development and application of biopolymer coatings to specialty green coffee beans: Influence on water content, color and sensory quality. LWT - Food Science and Technology, v. 96, p. 274-280, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Instrumentação. |
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| Registros recuperados : 8 | |
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