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Registros recuperados : 127 | |
101. | | BITTENCOURT, C. B.; SILVA, T. L. C. da; RODRIGUES NETO, J. C.; VIEIRA, L. R.; LEAO, A. P.; RIBEIRO, J. A. de A.; ABDELNUR, P. V.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T. Insights from a Multi-Omics Integration (MOI) study in oil palm (ELAEIS GUINEENSIS JACQ.) response to salinity and drought stresses. In: BRAZILIAN CONGRESS OF GENETICS, 67., 2022, Natal, RN. Porto Alegre: Sociedade Brasileira de Genética, 2022. p. 394 Biblioteca(s): Embrapa Agroenergia. |
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102. | | BITTENCOURT, C. B.; SILVA, T. L. C. da; RODRIGUES NETO, J. C.; VIEIRA, L. R.; LEAO, A. P.; RIBEIRO, J. A. de A.; ABDELNUR, P. V.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T. Insights from a Multi-Omics Integration (MOI) study in oil palm (ELAEIS GUINEENSIS JACQ.) response to salinity and drought stresses. In: BRAZILIAN CONGRESS OF GENETICS, 67., 2022, Natal, RN. p. 394 Biblioteca(s): Embrapa Meio-Norte. |
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103. | | SILVA, T. L. C. da; SILVA, V. N. B.; BRAGA, I. de O.; RODRIGUES NETO, J. C.; LEAO, A. P.; RIBEIRO, J. A. de A.; VALADARES, L. F.; ABDELNUR, P. V.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T. Integration of metabolomics and transcriptomics data to further characterize Gliricidia sepium (Jacq.) Kunth under high salinity stress. Plant Genome, e20182, 2021. Biblioteca(s): Embrapa Agroenergia; Embrapa Meio-Norte. |
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104. | | RODRIGUES NETO, J. C.; VARGAS, L. H. G.; RIBEIRO, J. A. de A.; SILVA, M. E. R. da; SOUZA JUNIOR, M. T.; RODRIGUES, C. M.; OLIVEIRA, A. E. de; ABDELNUR, P. V. Impressão digital metabólica de folhas de Elaeis guineensis em diferentes condições de preparo de amostra utilizando UHPLC-MS. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 3., 2016, Brasília, DF. Anais ... Brasília, DF: Embrapa, 2016. p. 237-245. Biblioteca(s): Embrapa Agroenergia. |
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105. | | FONTES, P. R.; RIBEIRO, J. A. de A.; COSTA, P. P. K. G.; DAMASO, M. C. T.; GONZALEZ, W. de A.; SANTOS, C. M. C. dos; ABDELNUR, P. V.; RODRIGUES, C. M. Development and validation of a HILIC-UPLC-ELSD method based on optimized chromatographic and detection parameters for the quantification of polyols from bioconversion processes. Analytical Methods, v. 8, p. 2048-2057, 2016. Biblioteca(s): Embrapa Agroenergia. |
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106. | | NETO, J. C. R.; RICCI-SILVA, M. E.; SANTOS, C. F. dos; COSTA, P. P. K. G.; RIBEIRO, J. A. de A.; SIQUEIRA, F. G. de; ABDELNUR, P. V. Desenvolvimento de protocolo analítico para identificação de proteínas em frutos de dendê utilizando MALDI(+)-TOF-MS In: CONGRESSO BRASILEIRO DE QUÍMICA, 55., 2015, Goiânia. Recursos renováveis: inovação e tecnologia. Rio de Janeiro: ABQ, 2015. Biblioteca(s): Embrapa Agroenergia. |
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107. | | TEIXEIRA, T. S.; RODRIGUES NETO, J. C.; MARTINS, P. A.; SILVA, E. A. da; COSTA, P. P. K. G.; SALUM, T. F. C.; SIQUEIRA, F. G. de; ABDELNUR, P. V. Desenvolvimento de protocolo para imagem química por espectrometria de massas de metabólitos-microrganismos. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 5., 2018, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2018. p. 46. Biblioteca(s): Embrapa Agroenergia. |
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108. | | BRAGA, Í. de O.; SILVA, T. L. C. da; SILVA, V. N. B.; RODRIGUES NETO, J. C.; RIBEIRO, J. A. de A.; ABDELNUR, P. V.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T. Deep untargeted metabolomics analysis to further characterize the adaptation response of Gliricidia sepium (Jacq.) Walp. to very high salinity stress. Frontiers in Plant Science, v. 13, Art. 869105, May, 2022. Biblioteca(s): Embrapa Agroenergia; Embrapa Meio-Norte. |
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109. | | CARNEIRO, A. A. J.; SOUTO, A. L.; SOUSA, G. P.; MENDES, T. D.; FAVARO, L. C. de L.; SALUM, T. F. C.; ABDELNUR, P. V.; RODRIGUES, C. M.; DAMASO, M. C. T. Evaluation of fungal strains for bioconversion of crude glycerin from palm oil and soybean into polyols. In: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 21.; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, 12., 2017, Aracajú, SE. [Anais ...]. São Paulo: Associação Brasileira de Engenharia Química, 2017. Não paginado. Biblioteca(s): Embrapa Agroenergia. |
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110. | | VARGAS, L. H. G.; RODRIGUES NETO, J. C.; RIBEIRO, J. A. de A.; RICCI-SILVA, M. E.; SOUZA JUNIOR, M. T.; RODRIGUES, C. M.; OLIVEIRA, A. E. DE; ABDELNUR, P. V. Metabolomics analysis of oil palm (Elaeis guineensis) leaf: evaluation of sample preparation steps using UHPLC?MS/MS. Metabolomics, v. 12, n. 10, artigo número 153, 2016. Biblioteca(s): Embrapa Agroenergia. |
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111. | | CAMPOS, C. G.; VERAS, H. C. T.; RIBEIRO, J. A. de A.; COSTA, P. P. K. G.; ARAÚJO, K. P.; RODRIGUES, C. M.; ALMEIDA, J. R. M. de; ABDELNUR, P. V. New protocol based on UHPLC-MS/MS for quantitation of metabolites in xylose-fermenting yeasts. Journal of the American Society for Mass Spectrometry, v. 28, n. 12, p. 2646-2657, 2017. p. 2646-2657 Biblioteca(s): Embrapa Agroenergia. |
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112. | | SILVA, V. N. B.; SILVA, T. L. C. da; FERREIRA, T. M. M.; RODRIGUES NETO, J. C.; LEAO, A. P.; RIBEIRO, J. A. de A.; ABDELNUR, P. V.; VALADARES, L. F.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T. Multi-omics analysis of young Portulaca Oleracea L. plants' responses to High NaCl doses reveals insights into pathways and genes responsive to salinity stress in this halophyte species. Phenomics, 2022. Published online: 15 June 2022. Biblioteca(s): Embrapa Agroenergia; Embrapa Meio-Norte. |
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113. | | SILVA, C. de O. G.; CUNHA, R. B.; CONCEIÇÃO, A. A.; ALMEIDA, E. G.; ZIED, D. C.; VIEIRA JUNIOR, W. G. V.; DIAS, E. S.; ISIKHUEMHEN, O. S.; ABDELNUR, P. V.; SIQUEIRA, F. G. de. Outdoor versus indoor cultivation: Effects on the metabolite profile of Agaricus subrufescens strains analyzed by untargeted metabolomics. Food Chemistry, v. 374, 131740, April 2022. 10 p. PDF: il. color. Biblioteca(s): Embrapa Agroenergia. |
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114. | | RODRIGUES, K. B.; MACÊDO, J. K. A.; TEIXEIRA, T.; BARROS, J. S.; ARAÚJO, A. C. B.; SANTOS, F. P.; QUIRINO, B. F.; BRASIL, B. dos S. A. F.; SALUM, T. F. C.; ABDELNUR, P. V.; FAVARO, L. C. de L. Recombinant expression of Thermobifida fusca E7 LPMO in Pichia pastoris and Escherichia coli and their functional characterization. Carbohydrate Research, v. 448, p. 175-181, 2017. Biblioteca(s): Embrapa Agroenergia. |
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115. | | RODRIGUES, K. B.; MACÊDO, J. K. A.; BARROS, J. S.; ARAÚJO, A. C. B.; SANTOS, F. P.; QUIRINO, B. F.; BRASIL, B. dos S. A. F.; SALUM, T. F. C.; ABDELNUR, P. V.; FAVARO, L. C. de L. Recombinant expression of Thermobifida fusca E7 LPMO in Pichia pastoris and Escherichia coli and their funstional characterization. In: SYMPOSIUM ON THE CHEMISTRY, BIOLOGY AND APPLICATION OF LYTIC POLYSACCHARIDE MONOOXYGENASES, 2016, Copenhagen, Denmark. [Proceedings ...]. Hellerup: Novo Nordisk Fonden, 2016. Não paginado. Resumo nº 20. Biblioteca(s): Embrapa Agroenergia. |
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116. | | PEREIRA, C. B.; SILVA, R. M. C. da; POLETTO, C. M.; CAMPOS, C. G.; RIBEIRO, J. A. de A.; RODRIGUES, C. M.; ABDELNUR, P. V.; ALMEIDA, J. R. M. de; DAMASO, M. C. T. Screening of microorganisms for production of aggregated-value chemicals from glycerin bioconversion. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 37., 2015, San Diego, California. [Resumos ...] Fairfax: Society for Industrial Microbiology and Biotechnology, 2015. Biblioteca(s): Embrapa Agroenergia. |
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117. | | PEREIRA. C. B.; SILVA, R. M. C.; POLETTO, C. M.; ARAÚJO, K. P.; RIBEIRO, J. A. de A.; RODRIGUES, C. M.; ABDELNUR, P. V.; ALMEIDA, J. R. M. de; DAMASO, M. C. T. Seleção de fungos filamentosos para produção de polióis a partir da bioconversão de glicerina bruta. In: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 20.; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, 11., 2015, Fortaleza, CE. [Anais ...]. São Paulo: Associação Brasileira de Engenharia Química, 2015. Biblioteca(s): Embrapa Agroenergia. |
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118. | | BAHIA, F. M.; ALMEIDA, G. C. de; ANDRADE, L. P. de; CAMPOS, C. G.; QUEIROZ, L. R.; SILVA, L. R. V. da; ABDELNUR, P. V.; CORRÊA, J. R.; BETTIGA, M.; PARACHIN, N. S. Rhamnolipids production from sucrose by engineered Saccharomyces cerevisiae. Scientific Reports, v. 8, artigo 2905, 2018. Biblioteca(s): Embrapa Agroenergia. |
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119. | | DINIZ, J. R.; CORREA, J. R.; MOREIRA, D. A.; FONTENELE, R. S.; OLIVEIRA, A. L.; ABDELNUR, P. V.; DUTRA, J. D. L.; FREIRE, R. O.; RODRIGUES, M. O.; NETO, B. A. D. Water-soluble Tb3+ and Eu3+ complexes with ionophilic (Ionically Tagged) ligands as fluorescence imaging probes. Inorganic Chemistry, v. 52, n. 17, p. 10199–10205, 2013. Biblioteca(s): Embrapa Agroenergia. |
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120. | | TEIXEIRA, T.; RODRIGUES, K.; MENDES, T. D.; SILVA, C.; SOUZA, A.; MENINO, G.; LO SCIUTO, D.; LOPES, R.; SANTANA, M.; MELLO, V.; RIBEIRO, J. A. de A.; SALUM, T. F. C.; DAMASO, M. C. T.; SIQUEIRA, F. G. de; RODRIGUES, D. de S.; ABDELNUR, P. V.; FAVARO, L. C. de L. Functional studies of Thermobifida fusca (E7) and Trichoderma reesei (Cel61A) LPMOs produced by Komagataella phaffii and their application in saccharification of sorghum and sugarcane bagasse. In: LPMO SYMPOSIUM, 2., 2018, Marseille, France. Proceedings ...]. [S.l]: LPMO, 2018. Biblioteca(s): Embrapa Agroenergia. |
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Registros recuperados : 127 | |
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Registro Completo
Biblioteca(s): |
Embrapa Agroenergia. |
Data corrente: |
23/03/2020 |
Data da última atualização: |
27/04/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
SILVA, C. de O. G.; TEIXEIRA, S. T.; RODRIGUES, K. B.; SOUZA, A. A.; MONCLARO, A. V.; MENDES, T. D.; RIBEIRO, J. A. de A.; SIQUEIRA, F. G. de; FAVARO, L. C. de L.; ABDELNUR, P. V. |
Afiliação: |
Caio de Oliveira Gorgulho Silva; Tallyta Santos Teixeira, Universidade Federal de Tocantins; Kelly Barreto Rodrigues; Amanda Araujo Souza; Antonielle Vieira Monclaro; THAIS DEMARCHI MENDES, CNPAE; JOSE ANTONIO DE AQUINO RIBEIRO, CNPAE; FELIX GONCALVES DE SIQUEIRA, CNPAE; LEIA CECILIA DE LIMA FAVARO, CNPAE; PATRICIA VERARDI ABDELNUR, CNPAE. |
Título: |
Combination of MALDI-TOF MS and UHPLC-ESI-MS for the characterization of lytic polysaccharide monooxygenase activity. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Analytical Methods, n. 2, 2020. |
Idioma: |
Inglês |
Conteúdo: |
Lytic polysaccharide monooxygenases (LPMOs) are redox enzymes of high biotechnological interest due to their capacity to degrade recalcitrant polysaccharides, such as cellulose, by an oxidative mechanism. The characterization of LPMOs is challenging since they generate a variety of catalytic products which include native oligosaccharides (non-oxidized) and oligosaccharides oxidized at the reducing end (C1 position), the non-reducing end (C4 position), or both ends, with different degrees of polymerization. Moreover, oxidized products exist in equilibrium with their hydrated forms, which further complicates their identification. The lack of commercial analytical standards for all these possible forms of oxidized oligosaccharides and the low concentration of these products make LPMO functional characterization dependent on advanced mass spectrometry techniques capable of identifying the profile of oxidized products. Here, a new approach for the characterization of cellulose-active LPMOs based on the combination of MALDI-TOF MS and hydrophilic interaction UHPLC-ESI-MS was proposed and optimized. The LPMO TrAA9A from Trichoderma reesei was used as a model enzyme to develop and test the MS methods. MALDI-TOF MS and UHPLC-ESI-MS methods were both capable of identifying putative C1, C4 and C1/C4 oxidized cello-oligosaccharides as well as their native counterparts generated after cellulose treatment with LPMO, which allows their utilization to characterize type I (C1-oxidizer), type II (C4-oxidizer) and type III (C1- and C4-oxidizer) LPMOs. Moreover, both methods were complementary since MALDI-TOF MS was capable of detecting oligosaccharides with higher degrees of polymerization (DP3?DP10), while UHPLC-ESI-MS allowed the evaluation of smaller oligosaccharides (DP1?DP5). The combined use of both methods offers a comprehensive description of LPMO catalytic products. MenosLytic polysaccharide monooxygenases (LPMOs) are redox enzymes of high biotechnological interest due to their capacity to degrade recalcitrant polysaccharides, such as cellulose, by an oxidative mechanism. The characterization of LPMOs is challenging since they generate a variety of catalytic products which include native oligosaccharides (non-oxidized) and oligosaccharides oxidized at the reducing end (C1 position), the non-reducing end (C4 position), or both ends, with different degrees of polymerization. Moreover, oxidized products exist in equilibrium with their hydrated forms, which further complicates their identification. The lack of commercial analytical standards for all these possible forms of oxidized oligosaccharides and the low concentration of these products make LPMO functional characterization dependent on advanced mass spectrometry techniques capable of identifying the profile of oxidized products. Here, a new approach for the characterization of cellulose-active LPMOs based on the combination of MALDI-TOF MS and hydrophilic interaction UHPLC-ESI-MS was proposed and optimized. The LPMO TrAA9A from Trichoderma reesei was used as a model enzyme to develop and test the MS methods. MALDI-TOF MS and UHPLC-ESI-MS methods were both capable of identifying putative C1, C4 and C1/C4 oxidized cello-oligosaccharides as well as their native counterparts generated after cellulose treatment with LPMO, which allows their utilization to characterize type I (C1-oxidizer), type... Mostrar Tudo |
Palavras-Chave: |
Caracterização de LPMOs. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02626naa a2200241 a 4500 001 2121326 005 2020-04-27 008 2020 bl uuuu u00u1 u #d 100 1 $aSILVA, C. de O. G. 245 $aCombination of MALDI-TOF MS and UHPLC-ESI-MS for the characterization of lytic polysaccharide monooxygenase activity.$h[electronic resource] 260 $c2020 520 $aLytic polysaccharide monooxygenases (LPMOs) are redox enzymes of high biotechnological interest due to their capacity to degrade recalcitrant polysaccharides, such as cellulose, by an oxidative mechanism. The characterization of LPMOs is challenging since they generate a variety of catalytic products which include native oligosaccharides (non-oxidized) and oligosaccharides oxidized at the reducing end (C1 position), the non-reducing end (C4 position), or both ends, with different degrees of polymerization. Moreover, oxidized products exist in equilibrium with their hydrated forms, which further complicates their identification. The lack of commercial analytical standards for all these possible forms of oxidized oligosaccharides and the low concentration of these products make LPMO functional characterization dependent on advanced mass spectrometry techniques capable of identifying the profile of oxidized products. Here, a new approach for the characterization of cellulose-active LPMOs based on the combination of MALDI-TOF MS and hydrophilic interaction UHPLC-ESI-MS was proposed and optimized. The LPMO TrAA9A from Trichoderma reesei was used as a model enzyme to develop and test the MS methods. MALDI-TOF MS and UHPLC-ESI-MS methods were both capable of identifying putative C1, C4 and C1/C4 oxidized cello-oligosaccharides as well as their native counterparts generated after cellulose treatment with LPMO, which allows their utilization to characterize type I (C1-oxidizer), type II (C4-oxidizer) and type III (C1- and C4-oxidizer) LPMOs. Moreover, both methods were complementary since MALDI-TOF MS was capable of detecting oligosaccharides with higher degrees of polymerization (DP3?DP10), while UHPLC-ESI-MS allowed the evaluation of smaller oligosaccharides (DP1?DP5). The combined use of both methods offers a comprehensive description of LPMO catalytic products. 653 $aCaracterização de LPMOs 700 1 $aTEIXEIRA, S. T. 700 1 $aRODRIGUES, K. B. 700 1 $aSOUZA, A. A. 700 1 $aMONCLARO, A. V. 700 1 $aMENDES, T. D. 700 1 $aRIBEIRO, J. A. de A. 700 1 $aSIQUEIRA, F. G. de 700 1 $aFAVARO, L. C. de L. 700 1 $aABDELNUR, P. V. 773 $tAnalytical Methods$gn. 2, 2020.
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