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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Suínos e Aves. |
Data corrente: |
09/03/2023 |
Data da última atualização: |
09/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SILVA, A. N. da; IBELLI, A. M. G.; SAVOLDI, I. R.; CANTAO, M. E.; ZANELLA, E. L.; MARQUES, M. G.; SILVA, M. V. G. B.; PEIXOTO, J. de O.; LEDUR, M. C.; LOPES, J. S.; VARGAS, J. E.; ZANELLA, R. |
Afiliação: |
ARTHUR NERY DA SILVA, Universidade de Passo Fundo; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; IGOR RICARDO SAVOLDI, Universidade do Contestado/Concórdia; MAURICIO EGIDIO CANTAO, CNPSA; ERALDO LOURENSO ZANELLA, UNIVERSIDADE; MARIANA GROKE MARQUES, CNPSA; MARCOS VINICIUS GUALBERTO B SILVA, CNPGL; JANE DE OLIVEIRA PEIXOTO, CNPSA; MONICA CORREA LEDUR, CNPSA; JADER SILVA LOPES, BRF/Curitiba; JOSÉ EDUARDO VARGAS, Universidade Federal do Paraná; RICARDO ZANELLA, Universidade de Passo Fundo. |
Título: |
Whole-exome sequencing indicated new candidate genes associated with unilateral cryptorchidism in pigs. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Sexual Development, 9 Feb 2023. |
DOI: |
10.1159/000528360 |
Idioma: |
Inglês |
Conteúdo: |
Abstract:
Introduction: Cryptorchidism is a hereditary anomaly characterized by the incomplete descent of one or both testicles to the scrotum. One of the challenges of this anomaly is that the retained testicle maintains its endocrine function. As a consequence, cryptorchid animals produce hormone-tainted meat in comparison to castrated animals and are likely to be more aggressive. Cryptorchidism can lead to reduced animal welfare outcomes and cause economic losses. Identifying genetic markers for cryptorchidism is an essential step toward mitigating these negative outcomes and may facilitate genome manipulation to reduce the occurrence of cryptorchidism. Attempts to identify such markers have used genome-wide association studies. Using whole-exome sequencing, we aimed to identify single nucleotide polymorphisms (SNPs) in the coding regions of cryptorchid pigs and to characterize functional pathways concerning these SNPs.
Methods: DNA was extracted and sequenced from 5 healthy and 5 cryptorchid animals from the Landrace breed, using the Illumina HiSeq 2500 platform. Data were pre-processed using the SeqyClean tool and further mapped against the swine reference genome (Sus scrofa 11.1) using BWA software. GATK was used to identify polymorphisms (SNPs and InDels), which were annotated using the VEP tool. Network prediction and gene ontology enrichment analysis were conducted using the Cytoscape platform, and STRING software was used for visualization.
Results: A total of 63 SNPs were identified across the genes PIGB, CCPG1, COMMD9, LDLRAD3, TRIM44, MYLPF, SEPTIN, ZNF48, TIA1, FAIM2, KRT18, FBP1, FBP2, CTSL, DAPK1, DHX8, GPR179, DEPDC1B, ENSSSCG00000049573, ENSSSCG00000016384, ENSSSCG00000022657, ENSSSCG00000038825, and ENSSSCG00000001229. Using pathway enrichment analyses and network prospection, we have identified the following significant adjusted p value threshold of 0.001 involved with the biological function pathways of estrogen signaling, cytoskeleton organization, and the pentose phosphate pathway.
Conclusion: Our data suggest the involvement of new SNPs and genes in developing cryptorchidism in pigs. However, further studies are needed to validate our results in a larger cohort population. Variations in the GPR179 gene, with implications at the protein level, may be associated with the appearance of this anomaly in the swine. Finally, we are showing that the estrogen signaling pathway may be involved in the pathophysiological mechanisms of this congenital anomaly as previously reported in GWAS. MenosAbstract:
Introduction: Cryptorchidism is a hereditary anomaly characterized by the incomplete descent of one or both testicles to the scrotum. One of the challenges of this anomaly is that the retained testicle maintains its endocrine function. As a consequence, cryptorchid animals produce hormone-tainted meat in comparison to castrated animals and are likely to be more aggressive. Cryptorchidism can lead to reduced animal welfare outcomes and cause economic losses. Identifying genetic markers for cryptorchidism is an essential step toward mitigating these negative outcomes and may facilitate genome manipulation to reduce the occurrence of cryptorchidism. Attempts to identify such markers have used genome-wide association studies. Using whole-exome sequencing, we aimed to identify single nucleotide polymorphisms (SNPs) in the coding regions of cryptorchid pigs and to characterize functional pathways concerning these SNPs.
Methods: DNA was extracted and sequenced from 5 healthy and 5 cryptorchid animals from the Landrace breed, using the Illumina HiSeq 2500 platform. Data were pre-processed using the SeqyClean tool and further mapped against the swine reference genome (Sus scrofa 11.1) using BWA software. GATK was used to identify polymorphisms (SNPs and InDels), which were annotated using the VEP tool. Network prediction and gene ontology enrichment analysis were conducted using the Cytoscape platform, and STRING software was used for visualization.
Results: A total of 63 ... Mostrar Tudo |
Palavras-Chave: |
Criptorquidismo; Sequenciamento completo do exoma; Whole-Exome Sequencing. |
Thesagro: |
Gene Marcador; Genética Animal; Marcador Genético; Porco; Suíno. |
Thesaurus Nal: |
Cryptorchidism. |
Categoria do assunto: |
-- |
Marc: |
LEADER 03604naa a2200373 a 4500 001 2152220 005 2023-03-09 008 2023 bl uuuu u00u1 u #d 024 7 $a10.1159/000528360$2DOI 100 1 $aSILVA, A. N. da 245 $aWhole-exome sequencing indicated new candidate genes associated with unilateral cryptorchidism in pigs.$h[electronic resource] 260 $c2023 520 $aAbstract: Introduction: Cryptorchidism is a hereditary anomaly characterized by the incomplete descent of one or both testicles to the scrotum. One of the challenges of this anomaly is that the retained testicle maintains its endocrine function. As a consequence, cryptorchid animals produce hormone-tainted meat in comparison to castrated animals and are likely to be more aggressive. Cryptorchidism can lead to reduced animal welfare outcomes and cause economic losses. Identifying genetic markers for cryptorchidism is an essential step toward mitigating these negative outcomes and may facilitate genome manipulation to reduce the occurrence of cryptorchidism. Attempts to identify such markers have used genome-wide association studies. Using whole-exome sequencing, we aimed to identify single nucleotide polymorphisms (SNPs) in the coding regions of cryptorchid pigs and to characterize functional pathways concerning these SNPs. Methods: DNA was extracted and sequenced from 5 healthy and 5 cryptorchid animals from the Landrace breed, using the Illumina HiSeq 2500 platform. Data were pre-processed using the SeqyClean tool and further mapped against the swine reference genome (Sus scrofa 11.1) using BWA software. GATK was used to identify polymorphisms (SNPs and InDels), which were annotated using the VEP tool. Network prediction and gene ontology enrichment analysis were conducted using the Cytoscape platform, and STRING software was used for visualization. Results: A total of 63 SNPs were identified across the genes PIGB, CCPG1, COMMD9, LDLRAD3, TRIM44, MYLPF, SEPTIN, ZNF48, TIA1, FAIM2, KRT18, FBP1, FBP2, CTSL, DAPK1, DHX8, GPR179, DEPDC1B, ENSSSCG00000049573, ENSSSCG00000016384, ENSSSCG00000022657, ENSSSCG00000038825, and ENSSSCG00000001229. Using pathway enrichment analyses and network prospection, we have identified the following significant adjusted p value threshold of 0.001 involved with the biological function pathways of estrogen signaling, cytoskeleton organization, and the pentose phosphate pathway. Conclusion: Our data suggest the involvement of new SNPs and genes in developing cryptorchidism in pigs. However, further studies are needed to validate our results in a larger cohort population. Variations in the GPR179 gene, with implications at the protein level, may be associated with the appearance of this anomaly in the swine. Finally, we are showing that the estrogen signaling pathway may be involved in the pathophysiological mechanisms of this congenital anomaly as previously reported in GWAS. 650 $aCryptorchidism 650 $aGene Marcador 650 $aGenética Animal 650 $aMarcador Genético 650 $aPorco 650 $aSuíno 653 $aCriptorquidismo 653 $aSequenciamento completo do exoma 653 $aWhole-Exome Sequencing 700 1 $aIBELLI, A. M. G. 700 1 $aSAVOLDI, I. R. 700 1 $aCANTAO, M. E. 700 1 $aZANELLA, E. L. 700 1 $aMARQUES, M. G. 700 1 $aSILVA, M. V. G. B. 700 1 $aPEIXOTO, J. de O. 700 1 $aLEDUR, M. C. 700 1 $aLOPES, J. S. 700 1 $aVARGAS, J. E. 700 1 $aZANELLA, R. 773 $tSexual Development, 9 Feb 2023.
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Embrapa Suínos e Aves (CNPSA) |
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Registro Completo
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
16/04/2024 |
Data da última atualização: |
16/04/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
MENESES, A. J. G.; POMPEU, R. C. F. F.; SALLES, H. O.; COSTA, C. dos S.; ROGERIO, M. C. P.; ANDRADE, I. N. A. de; FURTADO, R. N.; MEDEIROS, A. N. de; PEREIRA, P. L.; CÂNDIDO, M. J. D. |
Afiliação: |
ABNER JOSÉ GIRÃO MENESES, INSTITUTO FEDERAL DE EDUCAÇÃO, CIÊNCIA E TECNOLOGIA DO CEARÁ; ROBERTO CLAUDIO FERNANDES F POMPEU, CNPC; HEVILA OLIVEIRA SALLES FIGUEIREDO, CNPC; CLÉSIO DOS SANTOS COSTA, UNIVERSIDADE FEDERAL DO CEARÁ; MARCOS CLAUDIO PINHEIRO ROGERIO, CNPC; IGO RENAN ALBUQUERQUE DE ANDRADE, INSTITUTO FEDERAL DE EDUCAÇÃO, CIÊNCIA E TECNOLOGIA DO CEARÁ; RAFAEL NOGUEIRA FURTADO, INSTITUTO FEDERAL DO PIAUÍ; ARIOSVALDO NUNES DE MEDEIROS, UNIVERSIDADE FEDERAL DO CEARÁ; PATRÍCIO LEANDRO PEREIRA, UNIVERSIDADE ESTADUAL DO VALE DO ACARAÚ; MAGNO JOSÉ DUARTE CÂNDIDO, UNIVERSIDADE FEDERAL DO CEARÁ. |
Título: |
Nutritional parameters and productive performance of grazing sheep using castor bean cake as supplement or fertilizer. |
Ano de publicação: |
2024 |
Fonte/Imprenta: |
Tropical Animal Health and Production, v. 53, n. 3, article 121, Apr. 2024. |
DOI: |
10.1007/s11250-024-03967-3 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The objective was to evaluate the effect of detoxified castor bean replacing soybean meal in the concentrate diet or as nitrogen organic fertilizer replacing urea on intake and nutrient digestibility, blood parameters and productive performance of sheep finished on irrigated Tamani grass pasture under continuous stocking and variable stocking rate. The treatments were two concentrate diets: standard (ground corn and soybean meal) and alternative diet (ground corn and detoxified castor bean cake), and two nitrogen fertilizers: chemical (urea) and organic (fresh castor bean cake). The randomized complete block design was used in a 2 × 2 factorial arrangement with four replications (500 m² paddocks). Four sheep (2 castrated males and 2 females) were distributed in each experimental unit, totaling 64 animals with an average initial weight of 19.42 ± 3.6 kg. No effects (P > 0.05) were observed on the variables inherent to the evaluation of the pasture. The average stocking rate (SR) among treatments was 85.50 sheep/ha, equivalent to 9.87 Animal Units (AU)/ha. The alternative diet presented lower dry matter digestibility (62.71%), with no negative effects on nutrient intake and kidney parameters. Animals fed the standard and alternative diet showed average daily gain of 103.75 and 86.76 g/day, respectively. A finishing period of up to 100 days is recommended for sheep selected for production systems in semi-arid regions managed intensively on pasture. Detoxified castor bean cake did not alter nutrient intake, liver and kidney parameters of the sheep and can be used in pasture-based sheep farming. MenosAbstract: The objective was to evaluate the effect of detoxified castor bean replacing soybean meal in the concentrate diet or as nitrogen organic fertilizer replacing urea on intake and nutrient digestibility, blood parameters and productive performance of sheep finished on irrigated Tamani grass pasture under continuous stocking and variable stocking rate. The treatments were two concentrate diets: standard (ground corn and soybean meal) and alternative diet (ground corn and detoxified castor bean cake), and two nitrogen fertilizers: chemical (urea) and organic (fresh castor bean cake). The randomized complete block design was used in a 2 × 2 factorial arrangement with four replications (500 m² paddocks). Four sheep (2 castrated males and 2 females) were distributed in each experimental unit, totaling 64 animals with an average initial weight of 19.42 ± 3.6 kg. No effects (P > 0.05) were observed on the variables inherent to the evaluation of the pasture. The average stocking rate (SR) among treatments was 85.50 sheep/ha, equivalent to 9.87 Animal Units (AU)/ha. The alternative diet presented lower dry matter digestibility (62.71%), with no negative effects on nutrient intake and kidney parameters. Animals fed the standard and alternative diet showed average daily gain of 103.75 and 86.76 g/day, respectively. A finishing period of up to 100 days is recommended for sheep selected for production systems in semi-arid regions managed intensively on pasture. Detoxified castor b... Mostrar Tudo |
Palavras-Chave: |
Aspartate aminotransferase; Farelo de soja; Santa Inês. |
Thesagro: |
Alimento Para Animal; Caatinga; Glycine Max; Nutrição Animal; Ovino; Ricinus Communis. |
Thesaurus NAL: |
Brazil; Dietary supplements; Females; Nitrogen fertilizers; Ruminant nutrition; Semiarid zones; Sheep feeding; Urea; Weight gain. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02972naa a2200457 a 4500 001 2163638 005 2024-04-16 008 2024 bl uuuu u00u1 u #d 024 7 $a10.1007/s11250-024-03967-3$2DOI 100 1 $aMENESES, A. J. G. 245 $aNutritional parameters and productive performance of grazing sheep using castor bean cake as supplement or fertilizer.$h[electronic resource] 260 $c2024 520 $aAbstract: The objective was to evaluate the effect of detoxified castor bean replacing soybean meal in the concentrate diet or as nitrogen organic fertilizer replacing urea on intake and nutrient digestibility, blood parameters and productive performance of sheep finished on irrigated Tamani grass pasture under continuous stocking and variable stocking rate. The treatments were two concentrate diets: standard (ground corn and soybean meal) and alternative diet (ground corn and detoxified castor bean cake), and two nitrogen fertilizers: chemical (urea) and organic (fresh castor bean cake). The randomized complete block design was used in a 2 × 2 factorial arrangement with four replications (500 m² paddocks). Four sheep (2 castrated males and 2 females) were distributed in each experimental unit, totaling 64 animals with an average initial weight of 19.42 ± 3.6 kg. No effects (P > 0.05) were observed on the variables inherent to the evaluation of the pasture. The average stocking rate (SR) among treatments was 85.50 sheep/ha, equivalent to 9.87 Animal Units (AU)/ha. The alternative diet presented lower dry matter digestibility (62.71%), with no negative effects on nutrient intake and kidney parameters. Animals fed the standard and alternative diet showed average daily gain of 103.75 and 86.76 g/day, respectively. A finishing period of up to 100 days is recommended for sheep selected for production systems in semi-arid regions managed intensively on pasture. Detoxified castor bean cake did not alter nutrient intake, liver and kidney parameters of the sheep and can be used in pasture-based sheep farming. 650 $aBrazil 650 $aDietary supplements 650 $aFemales 650 $aNitrogen fertilizers 650 $aRuminant nutrition 650 $aSemiarid zones 650 $aSheep feeding 650 $aUrea 650 $aWeight gain 650 $aAlimento Para Animal 650 $aCaatinga 650 $aGlycine Max 650 $aNutrição Animal 650 $aOvino 650 $aRicinus Communis 653 $aAspartate aminotransferase 653 $aFarelo de soja 653 $aSanta Inês 700 1 $aPOMPEU, R. C. F. F. 700 1 $aSALLES, H. O. 700 1 $aCOSTA, C. dos S. 700 1 $aROGERIO, M. C. P. 700 1 $aANDRADE, I. N. A. de 700 1 $aFURTADO, R. N. 700 1 $aMEDEIROS, A. N. de 700 1 $aPEREIRA, P. L. 700 1 $aCÂNDIDO, M. J. D. 773 $tTropical Animal Health and Production$gv. 53, n. 3, article 121, Apr. 2024.
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