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Registro Completo |
Biblioteca(s): |
Embrapa Algodão. |
Data corrente: |
26/08/1993 |
Data da última atualização: |
26/08/1993 |
Autoria: |
CAVALCANTE, J. J.; SANTOS, R. F. dos; DUARTE, C. L. G. |
Título: |
Banco de dados de amendoim em casca. |
Ano de publicação: |
1991 |
Fonte/Imprenta: |
Campina Grande: EMBRAPA-CNPA, 1991. |
Páginas: |
19p |
Idioma: |
Português |
Palavras-Chave: |
Brasil; Groundnut; Harvest; Peanut; Production; Revenue. |
Thesagro: |
Amendoim; Arachis Hypogaea; Casca; Colheita; Produção; Rendimento. |
Thesaurus Nal: |
area; peeling. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00698nam a2200301 a 4500 001 1262797 005 1993-08-26 008 1991 bl uuuu u0uu1 u #d 100 1 $aCAVALCANTE, J. J. 245 $aBanco de dados de amendoim em casca. 260 $aCampina Grande: EMBRAPA-CNPA$c1991 300 $a19p 650 $aarea 650 $apeeling 650 $aAmendoim 650 $aArachis Hypogaea 650 $aCasca 650 $aColheita 650 $aProdução 650 $aRendimento 653 $aBrasil 653 $aGroundnut 653 $aHarvest 653 $aPeanut 653 $aProduction 653 $aRevenue 700 1 $aSANTOS, R. F. dos 700 1 $aDUARTE, C. L. G.
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Embrapa Algodão (CNPA) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
11/07/2013 |
Data da última atualização: |
02/08/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
OLIVEIRA, L. R.; RODRIGUES, E. P.; MARCELINO-GUIMARÃES, F. C.; OLIVEIRA, A. L. M.; HUNGRIA, M. |
Afiliação: |
LUCIANA RUANO OLIVEIRA, UEL; ELISETE PAINS RODRIGUES, UEL; FRANCISMAR CORREA MARCELINO-GUIMARÃES, CNPSO; ANDRÉ LUIZ MARTINEZ OLIVEIRA, UEL; MARIANGELA HUNGRIA, CNPSO. |
Título: |
Fast induction of biosynthetic polysaccharide genes lpxA, lpxE, and rkpI of Rhizobium sp. strain PRF 81 by common bean seed exudates is indicative of a key role in symbiosis. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Functional & Integrative Genomics, v. 13, n. 2, p. 275-283, Jun. 2013. |
ISSN: |
1438-7948 |
DOI: |
10.1007/s10142-013-0322-7 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Rhizobial surface polysaccharides (SPS) are, together with nodulation (Nod) factors, recognized as key molecules for establishment of rhizobia?legume symbiosis. In Rhizobium tropici, an important nitrogen-fixing symbiont of common bean (Phaseolus vulgaris L.), molecular structures and symbiotic roles of the SPS are poorly understood. In this study, Rhizobium sp. strain PRF 81 genes, belonging to the R. tropici group, were investigated: lpxA and lpxE, involved in biosynthesis and modification of the lipid-A anchor of lipopolysaccharide (LPS), and rkpI, involved in synthesis of a lipid carrier required for production of capsular polysaccharides (KPS). Reverse transcription quantitative PCR (RT-qPCR) analysis revealed, for the first time, that inducers released from common bean seeds strongly stimulated expression of all three SPS genes. When PRF 81 cells were grown for 48 h in the presence of seed exudates, twofold increases (p < 0.05) in the transcription levels of lpxE, lpxA, and rkpI genes were observed. However, higher increases (p < 0.05) in transcription rates, about 50-fold for lpxE and about 30-fold for lpxA and rkpI, were observed after only 5 min of incubation with common bean seed exudates. Evolutionary analyses revealed that lpxA and lpxE of PRF81 and of the type strain of R. tropici CIAT899Tclustered with orthologous Rhizobium radiobacter and were more related to R. etli and Rhizobium leguminosarum, while rkpI was closer to the Sinorhizobium sp. group. Upregulation of lpxE, lpxA, and rkpI genes suggests that seed exudates can modulate production of SPS of Rhizobium sp. PRF81, leading to cell wall changes necessary for symbiosis establishment. MenosAbstract: Rhizobial surface polysaccharides (SPS) are, together with nodulation (Nod) factors, recognized as key molecules for establishment of rhizobia?legume symbiosis. In Rhizobium tropici, an important nitrogen-fixing symbiont of common bean (Phaseolus vulgaris L.), molecular structures and symbiotic roles of the SPS are poorly understood. In this study, Rhizobium sp. strain PRF 81 genes, belonging to the R. tropici group, were investigated: lpxA and lpxE, involved in biosynthesis and modification of the lipid-A anchor of lipopolysaccharide (LPS), and rkpI, involved in synthesis of a lipid carrier required for production of capsular polysaccharides (KPS). Reverse transcription quantitative PCR (RT-qPCR) analysis revealed, for the first time, that inducers released from common bean seeds strongly stimulated expression of all three SPS genes. When PRF 81 cells were grown for 48 h in the presence of seed exudates, twofold increases (p < 0.05) in the transcription levels of lpxE, lpxA, and rkpI genes were observed. However, higher increases (p < 0.05) in transcription rates, about 50-fold for lpxE and about 30-fold for lpxA and rkpI, were observed after only 5 min of incubation with common bean seed exudates. Evolutionary analyses revealed that lpxA and lpxE of PRF81 and of the type strain of R. tropici CIAT899Tclustered with orthologous Rhizobium radiobacter and were more related to R. etli and Rhizobium leguminosarum, while rkpI was closer to the Si... Mostrar Tudo |
Thesagro: |
Feijão. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02449naa a2200205 a 4500 001 1961873 005 2017-08-02 008 2013 bl uuuu u00u1 u #d 022 $a1438-7948 024 7 $a10.1007/s10142-013-0322-7$2DOI 100 1 $aOLIVEIRA, L. R. 245 $aFast induction of biosynthetic polysaccharide genes lpxA, lpxE, and rkpI of Rhizobium sp. strain PRF 81 by common bean seed exudates is indicative of a key role in symbiosis.$h[electronic resource] 260 $c2013 520 $aAbstract: Rhizobial surface polysaccharides (SPS) are, together with nodulation (Nod) factors, recognized as key molecules for establishment of rhizobia?legume symbiosis. In Rhizobium tropici, an important nitrogen-fixing symbiont of common bean (Phaseolus vulgaris L.), molecular structures and symbiotic roles of the SPS are poorly understood. In this study, Rhizobium sp. strain PRF 81 genes, belonging to the R. tropici group, were investigated: lpxA and lpxE, involved in biosynthesis and modification of the lipid-A anchor of lipopolysaccharide (LPS), and rkpI, involved in synthesis of a lipid carrier required for production of capsular polysaccharides (KPS). Reverse transcription quantitative PCR (RT-qPCR) analysis revealed, for the first time, that inducers released from common bean seeds strongly stimulated expression of all three SPS genes. When PRF 81 cells were grown for 48 h in the presence of seed exudates, twofold increases (p < 0.05) in the transcription levels of lpxE, lpxA, and rkpI genes were observed. However, higher increases (p < 0.05) in transcription rates, about 50-fold for lpxE and about 30-fold for lpxA and rkpI, were observed after only 5 min of incubation with common bean seed exudates. Evolutionary analyses revealed that lpxA and lpxE of PRF81 and of the type strain of R. tropici CIAT899Tclustered with orthologous Rhizobium radiobacter and were more related to R. etli and Rhizobium leguminosarum, while rkpI was closer to the Sinorhizobium sp. group. Upregulation of lpxE, lpxA, and rkpI genes suggests that seed exudates can modulate production of SPS of Rhizobium sp. PRF81, leading to cell wall changes necessary for symbiosis establishment. 650 $aFeijão 700 1 $aRODRIGUES, E. P. 700 1 $aMARCELINO-GUIMARÃES, F. C. 700 1 $aOLIVEIRA, A. L. M. 700 1 $aHUNGRIA, M. 773 $tFunctional & Integrative Genomics$gv. 13, n. 2, p. 275-283, Jun. 2013.
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