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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
20/06/2014 |
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Data da última atualização: |
20/06/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MENEZES, E. F. de; SILVA, E. M. e; MELO, A. M. Y.; LEAO, P. C. de S.; MELO, N. F. de. |
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Afiliação: |
EIRYANNE FONSECA DE MENEZES; ELIENE MATOS E SILVA; ADRIANA MAYUMI YANO-MELO, UNIVASF; PATRICIA COELHO DE SOUZA LEAO, CPATSA; NATONIEL FRANKLIN DE MELO, CPATSA. |
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Título: |
Immature embryo rescue and in vitro development evaluation of intraspecific hybrids from Brazilian seedless grapevine Superior × Thompson clones. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
American Journal of Plant Sciences, Irvine, v. 5, p. 1956-1960, 2014. |
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Idioma: |
Inglês |
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Conteúdo: |
The fruit production for export is an economically significant activity in the Valley of São Francisco River, especially in the irrigated lands of Petrolina-PE/Juazeiro-BA, Brazil. The development of new genetic material most suitable to the tropical climate and the demands of the consumer market have led to the selection of new seedless grapes cultivars. In this case, the use of the embryo rescue technique has produced satisfactory results for obtaining such materials, especially in the semiarid region. This study aimed to evaluate the in vitro development of intraspecific hybrids of grapevine (Vitis vinifera L.), derived from the rescue of immature embryos resultant from the crossing of Superior Seedless and Thompson Seedless Brazilian clones. To establish and develop the cultivation, the culture media was supplemented with 30 g/L sucrose, 0.1 g/L myo-inositol, 0.002 g/L glycine, 0.1 mg/L indoleacetic acid (IAA), 6.5 g/L of agar, adjusted pH to 5.7. The experiment was evaluated after 90 days. The variables measured were: number of nodes, number of leaves, plant height (cm), number of roots and length (cm) of the root system and internodes. The period of 60 days of in vitro culture of ovules resulted in the highest values of embryos (about 50%), as well as better characterized developmental stages with higher germination (47.3%). The three types of hybrid grapes evaluated in micropropagation showed very similar values of the measured parameters, even having originated from embryos of different developmental stages. MenosThe fruit production for export is an economically significant activity in the Valley of São Francisco River, especially in the irrigated lands of Petrolina-PE/Juazeiro-BA, Brazil. The development of new genetic material most suitable to the tropical climate and the demands of the consumer market have led to the selection of new seedless grapes cultivars. In this case, the use of the embryo rescue technique has produced satisfactory results for obtaining such materials, especially in the semiarid region. This study aimed to evaluate the in vitro development of intraspecific hybrids of grapevine (Vitis vinifera L.), derived from the rescue of immature embryos resultant from the crossing of Superior Seedless and Thompson Seedless Brazilian clones. To establish and develop the cultivation, the culture media was supplemented with 30 g/L sucrose, 0.1 g/L myo-inositol, 0.002 g/L glycine, 0.1 mg/L indoleacetic acid (IAA), 6.5 g/L of agar, adjusted pH to 5.7. The experiment was evaluated after 90 days. The variables measured were: number of nodes, number of leaves, plant height (cm), number of roots and length (cm) of the root system and internodes. The period of 60 days of in vitro culture of ovules resulted in the highest values of embryos (about 50%), as well as better characterized developmental stages with higher germination (47.3%). The three types of hybrid grapes evaluated in micropropagation showed very similar values of the measured parameters, even having originated from ... Mostrar Tudo |
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Palavras-Chave: |
Cultivo in vitro; Melhoramento genético; Uva sem semente. |
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Thesagro: |
Genótipo; Uva; Vitis Vinifera. |
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Thesaurus Nal: |
Grapes. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/103919/1/Natoniel-2014.pdf
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Marc: |
LEADER 02339naa a2200253 a 4500
001 1988687
005 2014-06-20
008 2014 bl uuuu u00u1 u #d
100 1 $aMENEZES, E. F. de
245 $aImmature embryo rescue and in vitro development evaluation of intraspecific hybrids from Brazilian seedless grapevine Superior × Thompson clones.$h[electronic resource]
260 $c2014
520 $aThe fruit production for export is an economically significant activity in the Valley of São Francisco River, especially in the irrigated lands of Petrolina-PE/Juazeiro-BA, Brazil. The development of new genetic material most suitable to the tropical climate and the demands of the consumer market have led to the selection of new seedless grapes cultivars. In this case, the use of the embryo rescue technique has produced satisfactory results for obtaining such materials, especially in the semiarid region. This study aimed to evaluate the in vitro development of intraspecific hybrids of grapevine (Vitis vinifera L.), derived from the rescue of immature embryos resultant from the crossing of Superior Seedless and Thompson Seedless Brazilian clones. To establish and develop the cultivation, the culture media was supplemented with 30 g/L sucrose, 0.1 g/L myo-inositol, 0.002 g/L glycine, 0.1 mg/L indoleacetic acid (IAA), 6.5 g/L of agar, adjusted pH to 5.7. The experiment was evaluated after 90 days. The variables measured were: number of nodes, number of leaves, plant height (cm), number of roots and length (cm) of the root system and internodes. The period of 60 days of in vitro culture of ovules resulted in the highest values of embryos (about 50%), as well as better characterized developmental stages with higher germination (47.3%). The three types of hybrid grapes evaluated in micropropagation showed very similar values of the measured parameters, even having originated from embryos of different developmental stages.
650 $aGrapes
650 $aGenótipo
650 $aUva
650 $aVitis Vinifera
653 $aCultivo in vitro
653 $aMelhoramento genético
653 $aUva sem semente
700 1 $aSILVA, E. M. e
700 1 $aMELO, A. M. Y.
700 1 $aLEAO, P. C. de S.
700 1 $aMELO, N. F. de
773 $tAmerican Journal of Plant Sciences, Irvine$gv. 5, p. 1956-1960, 2014.
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Embrapa Semiárido (CPATSA) |
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