| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Gado de Corte. |
|
Data corrente: |
27/02/2014 |
|
Data da última atualização: |
21/02/2018 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
SILVA, G. T.; SILVA, C. B. C.; JANK, L.; SOUZA, A. P. |
|
Afiliação: |
GUILHERME TOLEDO-SILVA, Molecular Biology Center and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, São Paulo, Brazil.; CLAUDIO BENICIO CARDOSO-SILVA, Molecular Biology Center and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, São Paulo, Brazil; LIANA JANK, CNPGC; ANETE PEREIRA SOUZA, Molecular Biology Center and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, São Paulo, Brazil; Department of Plant Biology, Biology Institute, University of Campinas (UNICAMP), Campinas, São Paulo, BraziL. |
|
Título: |
De novo transcriptome assembly for the tropical grass Panicum maximum Jacq. |
|
Ano de publicação: |
2013 |
|
Fonte/Imprenta: |
PLOS ONE, v. 8, n. 7, p. 1-10, july 2013 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Guinea grass (Panicum maximum Jacq.) is a tropical African grass often used to feed beef cattle, which is an important economic activity in Brazil. Brazil is the leader in global meat exportation because of its exclusively pasture-raised bovine herds. Guinea grass also has potential uses in bioenergy production due to its elevated biomass generation through the C4 photosynthesis pathway. We generated approximately 13 Gb of data from Illumina sequencing of P. maximum leaves. Four different genotypes were sequenced, and the combined reads were assembled de novo into 38,192 unigenes and annotated; approximately 63% of the unigenes had homology to other proteins in the NCBI non-redundant protein database. Functional classification through COG (Clusters of Orthologous Groups), GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) analyses showed that the unigenes from Guinea grass leaves are involved in a wide range of biological processes and metabolic pathways, including C4 photosynthesis and lignocellulose generation, which are important for cattle grazing and bioenergy production. The most abundant transcripts were involved in carbon fixation, photosynthesis, RNA translation and heavy metal cellular homeostasis. Finally, we identified a number of potential molecular markers, including 5,035 microsatellites (SSRs) and 346,456 single nucleotide polymorphisms (SNPs). To the best of our knowledge, this is the first study to characterize the complete leaf transcriptome of P. maximum using highthroughput sequencing. The biological information provided here will aid in gene expression studies and marker-assisted selection-based breeding research in tropical grasses. MenosGuinea grass (Panicum maximum Jacq.) is a tropical African grass often used to feed beef cattle, which is an important economic activity in Brazil. Brazil is the leader in global meat exportation because of its exclusively pasture-raised bovine herds. Guinea grass also has potential uses in bioenergy production due to its elevated biomass generation through the C4 photosynthesis pathway. We generated approximately 13 Gb of data from Illumina sequencing of P. maximum leaves. Four different genotypes were sequenced, and the combined reads were assembled de novo into 38,192 unigenes and annotated; approximately 63% of the unigenes had homology to other proteins in the NCBI non-redundant protein database. Functional classification through COG (Clusters of Orthologous Groups), GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) analyses showed that the unigenes from Guinea grass leaves are involved in a wide range of biological processes and metabolic pathways, including C4 photosynthesis and lignocellulose generation, which are important for cattle grazing and bioenergy production. The most abundant transcripts were involved in carbon fixation, photosynthesis, RNA translation and heavy metal cellular homeostasis. Finally, we identified a number of potential molecular markers, including 5,035 microsatellites (SSRs) and 346,456 single nucleotide polymorphisms (SNPs). To the best of our knowledge, this is the first study to characterize the complete leaf transcrip... Mostrar Tudo |
|
Thesagro: |
Alimento animal; Carne; Graminea tropical; Panicum maximum. |
|
Thesaurus Nal: |
Beef cattle; Megathyrsus maximus. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/98451/1/deb-pone.0070781-1..10-fetchObject.action.pdf
|
|
Marc: |
LEADER 02333naa a2200229 a 4500
001 1981465
005 2018-02-21
008 2013 bl uuuu u00u1 u #d
100 1 $aSILVA, G. T.
245 $aDe novo transcriptome assembly for the tropical grass Panicum maximum Jacq.$h[electronic resource]
260 $c2013
520 $aGuinea grass (Panicum maximum Jacq.) is a tropical African grass often used to feed beef cattle, which is an important economic activity in Brazil. Brazil is the leader in global meat exportation because of its exclusively pasture-raised bovine herds. Guinea grass also has potential uses in bioenergy production due to its elevated biomass generation through the C4 photosynthesis pathway. We generated approximately 13 Gb of data from Illumina sequencing of P. maximum leaves. Four different genotypes were sequenced, and the combined reads were assembled de novo into 38,192 unigenes and annotated; approximately 63% of the unigenes had homology to other proteins in the NCBI non-redundant protein database. Functional classification through COG (Clusters of Orthologous Groups), GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) analyses showed that the unigenes from Guinea grass leaves are involved in a wide range of biological processes and metabolic pathways, including C4 photosynthesis and lignocellulose generation, which are important for cattle grazing and bioenergy production. The most abundant transcripts were involved in carbon fixation, photosynthesis, RNA translation and heavy metal cellular homeostasis. Finally, we identified a number of potential molecular markers, including 5,035 microsatellites (SSRs) and 346,456 single nucleotide polymorphisms (SNPs). To the best of our knowledge, this is the first study to characterize the complete leaf transcriptome of P. maximum using highthroughput sequencing. The biological information provided here will aid in gene expression studies and marker-assisted selection-based breeding research in tropical grasses.
650 $aBeef cattle
650 $aMegathyrsus maximus
650 $aAlimento animal
650 $aCarne
650 $aGraminea tropical
650 $aPanicum maximum
700 1 $aSILVA, C. B. C.
700 1 $aJANK, L.
700 1 $aSOUZA, A. P.
773 $tPLOS ONE$gv. 8, n. 7, p. 1-10, july 2013
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Corte (CNPGC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 16 | |
| 2. |  | MORAES, B. F. X. de; ANDRADE, V. T.; TEIXEIRA, L. G. V.; TEIXEIRA, A. L.; GONÇALVCES, F. M. A.; REZENDE, J. C. de. Análise de Componentes Principais em Caracteres Morfológicos de Coffea arabica L. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 6., 2011, Búzios. Panorama atual e perspectivas do melhoramento de plantas no Brasil. [Búzios]: SBMP, 2011. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Rondônia. |
|    |
| 9. | | RODRIGUES, G. S.; RODRIGUES, I. A.; BUSCHINELLI, C. C. de A.; NEVES, M. C. M.; FREITAS, G. L.; ANTUNES, L. R.; RODOVALHO, R. B. Gestão ambiental das atividades rurais na APA da Barra do Rio Mamanguape (PB). In: CONGRESSO BRASILEIRO DE UNIDADES DE CONSERVAÇÃO, 5., 2007, Foz do Iguaçú. Anais: Trabalhos técnicos. Foz do Iguaçú, 2007. p.51-52.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
| |
| 12. | | AMARAL, F. C. S. do; CARVALHO JUNIOR, W. de; PEREIRA, N. R.; CHAGAS, C. da S.; BHERING, S. B.; ZARONI, M. J.; MACEDO, J. R. de; SANTOS, H. G. dos; SILVA, E. F. da; GONCALVES, A. O.; MARTINS, J. S.; AGLIO, M. L. D.; DART, R. de O.; AMORIM, A. M.; LOPES, C. H. L.; FIALHO, A. A.; SOARES SOBRINHO, J. Mapa do zoneamento agroecológico para maracujá no município de São Gabriel do Oeste. Rio de Janeiro: Embrapa Solos, 2010.| Tipo: Monitoramento/Zoneamento |
| Biblioteca(s): Embrapa Solos. |
|  |
| Registros recuperados : 16 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
