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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
27/01/2014 |
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Data da última atualização: |
09/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
COSTA, F. M.; SCHIAVO, J. A.; BRASIL, M. S.; LEITE, J.; XAVIER, G. R.; FERNANDES JUNIOR, P. I. |
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Afiliação: |
Universidade Estadual do Mato Grosso do Sul; Universidade Estadual do Mato Grosso do Sul; Universidade Federal do Mato Grosso do Sul; Universidade Federal Rural do Rio de Janeiro; GUSTAVO RIBEIRO XAVIER, CNPAB; PAULO IVAN FERNANDES JUNIOR, CPATSA. |
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Título: |
Phenotypic and molecular finger printing of fast growing rhizobia of field-grown pigeonpea from the eastern edge of the Brazilian Pantanal. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 13, n. 1, p. 469-482 2014. |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to evaluate the diversity of rhizobial isolates obtained from root nodules of pigeonpea plants grown at the eastern edge of the Brazilian Pantanal. The bacterial isolates were isolated from root nodules from field-growing pigeonpea grown in two rural settlements of the Aquidauana municipality. The bacterial isolates were characterized phenotypically by means of cultural characterization, intrinsic antibiotic resistance (IAR), salt and high incubation temperature tolerance, and amylolytic and cellulolytic activities. The molecular characterization of the bacterial isolates was carried out using amplified ribosomal DNA restriction analysis (ARDRA) and Box-polymerase chain reaction (PCR) techniques. In addition, the symbiotic performance of selected rhizobial isolates was evaluated in a greenhouse experiment using sterile substrate. The phenotypic characterization revealed that the bacterial strains obtained from pigeonpea root nodules presented characteristics that are uncommon among rhizobial isolates, indicating the presence of new species nodulating the pigeonpea plants in the Brazilian Pantanal. The molecular fingerprinting of these bacterial isolates also showed a highly diverse collection, with both techniques revealing less than 25% similarity among bacterial isolates. The evaluation of symbiotic performance also indicated the presence of microorganisms with high potential to increase the growth and nitrogen content at the shoots of pigeonpea plants. The results obtained in this study indicate the presence of a highly diversified rhizobial community nodulating the pigeonpea at the eastern edge of the Brazilian Pantanal. MenosThe aim of this study was to evaluate the diversity of rhizobial isolates obtained from root nodules of pigeonpea plants grown at the eastern edge of the Brazilian Pantanal. The bacterial isolates were isolated from root nodules from field-growing pigeonpea grown in two rural settlements of the Aquidauana municipality. The bacterial isolates were characterized phenotypically by means of cultural characterization, intrinsic antibiotic resistance (IAR), salt and high incubation temperature tolerance, and amylolytic and cellulolytic activities. The molecular characterization of the bacterial isolates was carried out using amplified ribosomal DNA restriction analysis (ARDRA) and Box-polymerase chain reaction (PCR) techniques. In addition, the symbiotic performance of selected rhizobial isolates was evaluated in a greenhouse experiment using sterile substrate. The phenotypic characterization revealed that the bacterial strains obtained from pigeonpea root nodules presented characteristics that are uncommon among rhizobial isolates, indicating the presence of new species nodulating the pigeonpea plants in the Brazilian Pantanal. The molecular fingerprinting of these bacterial isolates also showed a highly diverse collection, with both techniques revealing less than 25% similarity among bacterial isolates. The evaluation of symbiotic performance also indicated the presence of microorganisms with high potential to increase the growth and nitrogen content at the shoots of pigeonpea p... Mostrar Tudo |
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Palavras-Chave: |
Diversidade; Ecologia microbiana; Feijão guandu; Fixação biológica de nitrogênio; Nódulos radiculares; Rizóbio. |
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Thesagro: |
Bactéria; Feijão; Fixação de Nitrogênio. |
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Thesaurus Nal: |
Beans; Genetic disorders; Plant genetics. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/95854/1/Paulo-Ivan.pdf
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Marc: |
LEADER 02669naa a2200325 a 4500
001 1977492
005 2023-01-09
008 2014 bl uuuu u00u1 u #d
100 1 $aCOSTA, F. M.
245 $aPhenotypic and molecular finger printing of fast growing rhizobia of field-grown pigeonpea from the eastern edge of the Brazilian Pantanal.$h[electronic resource]
260 $c2014
520 $aThe aim of this study was to evaluate the diversity of rhizobial isolates obtained from root nodules of pigeonpea plants grown at the eastern edge of the Brazilian Pantanal. The bacterial isolates were isolated from root nodules from field-growing pigeonpea grown in two rural settlements of the Aquidauana municipality. The bacterial isolates were characterized phenotypically by means of cultural characterization, intrinsic antibiotic resistance (IAR), salt and high incubation temperature tolerance, and amylolytic and cellulolytic activities. The molecular characterization of the bacterial isolates was carried out using amplified ribosomal DNA restriction analysis (ARDRA) and Box-polymerase chain reaction (PCR) techniques. In addition, the symbiotic performance of selected rhizobial isolates was evaluated in a greenhouse experiment using sterile substrate. The phenotypic characterization revealed that the bacterial strains obtained from pigeonpea root nodules presented characteristics that are uncommon among rhizobial isolates, indicating the presence of new species nodulating the pigeonpea plants in the Brazilian Pantanal. The molecular fingerprinting of these bacterial isolates also showed a highly diverse collection, with both techniques revealing less than 25% similarity among bacterial isolates. The evaluation of symbiotic performance also indicated the presence of microorganisms with high potential to increase the growth and nitrogen content at the shoots of pigeonpea plants. The results obtained in this study indicate the presence of a highly diversified rhizobial community nodulating the pigeonpea at the eastern edge of the Brazilian Pantanal.
650 $aBeans
650 $aGenetic disorders
650 $aPlant genetics
650 $aBactéria
650 $aFeijão
650 $aFixação de Nitrogênio
653 $aDiversidade
653 $aEcologia microbiana
653 $aFeijão guandu
653 $aFixação biológica de nitrogênio
653 $aNódulos radiculares
653 $aRizóbio
700 1 $aSCHIAVO, J. A.
700 1 $aBRASIL, M. S.
700 1 $aLEITE, J.
700 1 $aXAVIER, G. R.
700 1 $aFERNANDES JUNIOR, P. I.
773 $tGenetics and Molecular Research$gv. 13, n. 1, p. 469-482 2014.
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Embrapa Semiárido (CPATSA) |
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