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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
22/01/2014 |
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Data da última atualização: |
09/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TEIXEIRA, E. W.; SANTOS, L. G. DOS; SATTLER, A.; MESSAGE, D.; ALVES, M. L. T. M. F.; MARTINS, M. F.; GRASSI-SELLA, M. L. |
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Afiliação: |
ERICA WEINSTEIN TEIXEIRA, APTA; LUBIANE GUIMARAES DOS SANTOS, UFV; ARONI SATTLER, UFV; DEJAIR MESSAGE, UFERSA; MARIA LUISA T. M. F. ALVES, APTA; MARTA FONSECA MARTINS, CNPGL; MARINA LOPES GRASSI-SELLA, USP. |
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Título: |
Nosema ceranae has been present in Brazil for more than three decades infecting africanized honey bees. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Journal of Invertebrate Pathology, v. 114, n. 3, p. 250-254, 2013. |
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DOI: |
https://doi.org/10.1016/j.jip.2013.09.002 |
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Idioma: |
Inglês |
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Conteúdo: |
Until the mid-1990s, the only microsporidium known to infect bees of the genus Apis was Nosema apis. A second species, Nosema ceranae, was first identified in 1996 from Asian honey bees; it is postulated that this parasite was transmitted from the Asian honey bee, Apis cerana, to the European honey bee, Apis mellifera. Currently, N. ceranae is found on all continents and has often been associated with honey bee colony collapse and other reports of high bee losses. Samples of Africanized drones collected in 1979, preserved in alcohol, were analyzed by light microscopy to count spores and were subjected to DNA extraction, after which duplex PCR was conducted. All molecular analyses (triplicate) indicated that the drones were infected with both N. ceranae and N. apis. PCR products were sequenced and matched to sequences reported in the GenBank (Acc. Nos. JQ639316.1 and JQ639301.1). The venation pattern of the wings of these males was compared to those of the current population living in the same area and with the pattern of drones collected in 1968 from Ribeirão Preto, SP, Brazil, from a location close to where African swarms first escaped in 1956. The morphometric results indicated that the population collected in 1979 was significantly different from the current living population, confirming its antiquity. Considering that the use of molecular tools for identifying Nosema species is relatively recent, it is possible that previous reports of infections (which used only light microscopy, without ultrastructural analysis) wrongly identified N. ceranae as N. apis. Although we can conclude that N. ceranae has been affecting Africanized honeybees in Brazil for at least 34 years, the impact of this pathogen remains unclear. MenosUntil the mid-1990s, the only microsporidium known to infect bees of the genus Apis was Nosema apis. A second species, Nosema ceranae, was first identified in 1996 from Asian honey bees; it is postulated that this parasite was transmitted from the Asian honey bee, Apis cerana, to the European honey bee, Apis mellifera. Currently, N. ceranae is found on all continents and has often been associated with honey bee colony collapse and other reports of high bee losses. Samples of Africanized drones collected in 1979, preserved in alcohol, were analyzed by light microscopy to count spores and were subjected to DNA extraction, after which duplex PCR was conducted. All molecular analyses (triplicate) indicated that the drones were infected with both N. ceranae and N. apis. PCR products were sequenced and matched to sequences reported in the GenBank (Acc. Nos. JQ639316.1 and JQ639301.1). The venation pattern of the wings of these males was compared to those of the current population living in the same area and with the pattern of drones collected in 1968 from Ribeirão Preto, SP, Brazil, from a location close to where African swarms first escaped in 1956. The morphometric results indicated that the population collected in 1979 was significantly different from the current living population, confirming its antiquity. Considering that the use of molecular tools for identifying Nosema species is relatively recent, it is possible that previous reports of infections (which used only light m... Mostrar Tudo |
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Palavras-Chave: |
Patologia; PCR. |
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Thesagro: |
Abelha Africana; Nosema Apis. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02528naa a2200253 a 4500
001 1977041
005 2022-08-09
008 2013 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.jip.2013.09.002$2DOI
100 1 $aTEIXEIRA, E. W.
245 $aNosema ceranae has been present in Brazil for more than three decades infecting africanized honey bees.$h[electronic resource]
260 $c2013
520 $aUntil the mid-1990s, the only microsporidium known to infect bees of the genus Apis was Nosema apis. A second species, Nosema ceranae, was first identified in 1996 from Asian honey bees; it is postulated that this parasite was transmitted from the Asian honey bee, Apis cerana, to the European honey bee, Apis mellifera. Currently, N. ceranae is found on all continents and has often been associated with honey bee colony collapse and other reports of high bee losses. Samples of Africanized drones collected in 1979, preserved in alcohol, were analyzed by light microscopy to count spores and were subjected to DNA extraction, after which duplex PCR was conducted. All molecular analyses (triplicate) indicated that the drones were infected with both N. ceranae and N. apis. PCR products were sequenced and matched to sequences reported in the GenBank (Acc. Nos. JQ639316.1 and JQ639301.1). The venation pattern of the wings of these males was compared to those of the current population living in the same area and with the pattern of drones collected in 1968 from Ribeirão Preto, SP, Brazil, from a location close to where African swarms first escaped in 1956. The morphometric results indicated that the population collected in 1979 was significantly different from the current living population, confirming its antiquity. Considering that the use of molecular tools for identifying Nosema species is relatively recent, it is possible that previous reports of infections (which used only light microscopy, without ultrastructural analysis) wrongly identified N. ceranae as N. apis. Although we can conclude that N. ceranae has been affecting Africanized honeybees in Brazil for at least 34 years, the impact of this pathogen remains unclear.
650 $aAbelha Africana
650 $aNosema Apis
653 $aPatologia
653 $aPCR
700 1 $aSANTOS, L. G. DOS
700 1 $aSATTLER, A.
700 1 $aMESSAGE, D.
700 1 $aALVES, M. L. T. M. F.
700 1 $aMARTINS, M. F.
700 1 $aGRASSI-SELLA, M. L.
773 $tJournal of Invertebrate Pathology$gv. 114, n. 3, p. 250-254, 2013.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Uva e Vinho. |
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Data corrente: |
14/03/2023 |
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Data da última atualização: |
24/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 4 |
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Autoria: |
FAJARDO, T. V. M.; NHANI JUNIOR, A.; NICKEL, O. |
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Afiliação: |
THOR VINICIUS MARTINS FAJARDO, CNPUV; ANTONIO NHANI JUNIOR, CNPTIA; OSMAR NICKEL, CNPUV. |
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Título: |
Analysis of virome by high-throughput sequencing disclosed multiple infection in a single grapevine plant. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Ciência Rural, v. 53, n. 10, e20220284, 2023. |
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Idioma: |
Inglês |
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Conteúdo: |
Among phytosanitary problems of the grapevine, viruses stand out for their capacity of reducing the quality and yield of grapes. however, detecting and identifying viral infections in grapevines can be challenging. This study performed a high throughput sequencing (hTS) of the viral pathogens present in a vine showing virus-like symptoms to elucidate the etiology. hTS analysis reported in a hybrid grapevine with mild curling down of leaf edges, the presence of four viruses and viroids, which were probably implicated in the observed symptoms. The determined complete genomes showed high genetic identities with previously characterized isolates of homologous pathogens. Key words: diagnosis, symptoms, hTS, diversity, virus. Resumo: Dentre os problemas fitossanitários da videira, os vírus se destacam pela capacidade de reduzir a qualidade e o rendimento da uva. No entanto, detectar e identificar infecções virais em videiras pode ser um desafio. O objetivo do estudo foi realizar um sequenciamento de alto rendimento (hTS) para determinar os patógenos virais presentes em uma videira com sintomas de virose e elucidar a etiologia. com o hTS foi detectada, em uma videira híbrida com leve enrolamento dos bordos foliares, a presença de quatro vírus e viroides, os quais provavelmente estavam implicados com os sintomas observados. Os genomas completos determinados mostraram altas identidades genéticas com isolados previamente caracterizados de patógenos homólogos. Palavras-chave: diagnose, sintomas, hTS, diversidade, vírus. MenosAmong phytosanitary problems of the grapevine, viruses stand out for their capacity of reducing the quality and yield of grapes. however, detecting and identifying viral infections in grapevines can be challenging. This study performed a high throughput sequencing (hTS) of the viral pathogens present in a vine showing virus-like symptoms to elucidate the etiology. hTS analysis reported in a hybrid grapevine with mild curling down of leaf edges, the presence of four viruses and viroids, which were probably implicated in the observed symptoms. The determined complete genomes showed high genetic identities with previously characterized isolates of homologous pathogens. Key words: diagnosis, symptoms, hTS, diversity, virus. Resumo: Dentre os problemas fitossanitários da videira, os vírus se destacam pela capacidade de reduzir a qualidade e o rendimento da uva. No entanto, detectar e identificar infecções virais em videiras pode ser um desafio. O objetivo do estudo foi realizar um sequenciamento de alto rendimento (hTS) para determinar os patógenos virais presentes em uma videira com sintomas de virose e elucidar a etiologia. com o hTS foi detectada, em uma videira híbrida com leve enrolamento dos bordos foliares, a presença de quatro vírus e viroides, os quais provavelmente estavam implicados com os sintomas observados. Os genomas completos determinados mostraram altas identidades genéticas com isolados previamente caracterizados de patógenos homólogos. Palavras-chave: diagnose,... Mostrar Tudo |
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Palavras-Chave: |
Diagnose; Diagnosis; Diversidade; Diversity; HTS; Sequenciamento de alto rendimento; Sintomas; Symptoms; Videira. |
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Thesagro: |
Vírus. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1152340/1/Fajardo-2023-CienciaRural.pdf
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Marc: |
LEADER 02281naa a2200265 a 4500
001 2152340
005 2023-03-24
008 2023 bl uuuu u00u1 u #d
100 1 $aFAJARDO, T. V. M.
245 $aAnalysis of virome by high-throughput sequencing disclosed multiple infection in a single grapevine plant.$h[electronic resource]
260 $c2023
520 $aAmong phytosanitary problems of the grapevine, viruses stand out for their capacity of reducing the quality and yield of grapes. however, detecting and identifying viral infections in grapevines can be challenging. This study performed a high throughput sequencing (hTS) of the viral pathogens present in a vine showing virus-like symptoms to elucidate the etiology. hTS analysis reported in a hybrid grapevine with mild curling down of leaf edges, the presence of four viruses and viroids, which were probably implicated in the observed symptoms. The determined complete genomes showed high genetic identities with previously characterized isolates of homologous pathogens. Key words: diagnosis, symptoms, hTS, diversity, virus. Resumo: Dentre os problemas fitossanitários da videira, os vírus se destacam pela capacidade de reduzir a qualidade e o rendimento da uva. No entanto, detectar e identificar infecções virais em videiras pode ser um desafio. O objetivo do estudo foi realizar um sequenciamento de alto rendimento (hTS) para determinar os patógenos virais presentes em uma videira com sintomas de virose e elucidar a etiologia. com o hTS foi detectada, em uma videira híbrida com leve enrolamento dos bordos foliares, a presença de quatro vírus e viroides, os quais provavelmente estavam implicados com os sintomas observados. Os genomas completos determinados mostraram altas identidades genéticas com isolados previamente caracterizados de patógenos homólogos. Palavras-chave: diagnose, sintomas, hTS, diversidade, vírus.
650 $aVírus
653 $aDiagnose
653 $aDiagnosis
653 $aDiversidade
653 $aDiversity
653 $aHTS
653 $aSequenciamento de alto rendimento
653 $aSintomas
653 $aSymptoms
653 $aVideira
700 1 $aNHANI JUNIOR, A.
700 1 $aNICKEL, O.
773 $tCiência Rural$gv. 53, n. 10, e20220284, 2023.
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Embrapa Uva e Vinho (CNPUV) |
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