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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Pecuária Sudeste. |
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Data corrente: |
24/06/2013 |
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Data da última atualização: |
12/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MELO, P. de C.; FERREIRA, L. M.; NADER FILHO, A.; ZAFALON, L. F.; VICENTE, H. I. G.; SOUZA, V. de. |
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Afiliação: |
POLIANA DE CASTRO MELO, Universidade Estadual Paulista; LUCIANO MENEZES FERREIRA, Universidade Estadual Paulista; ANTONIO NADER FILHO, Universidade Estadual Paulista; LUIZ FRANCISCO ZAFALON, CPPSE; HINIG ISA GODOY VICENTE, Secretaria de Agricultura e Abastecimento do Estado de São Paulo; VIVIANE DE SOUZA, CNPC. |
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Título: |
Comparison of methods for the detection of biofilm formation by Staphylococcus aureus isolated from bovine subclinical mastitis. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, v. 44, n. 1, p. 119-124, 2013. |
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DOI: |
https://doi.org/10.1590/S1517-83822013005000031 |
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Idioma: |
Inglês
Português |
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Conteúdo: |
Abstract: Biofilm formation is considered to be a selective advantage for Staphylococcus aureus mastitis isolates by facilitating bacterial persistence in the udder. It requires attachment to mammary epithelium, proliferation and accumulation of cells in multilayers. The objective of this study was to determine the sensitivity and specificity of three techniques for the detection of S. aureus biofilm-positive strains. Two phenotypic tests, including growth on microtitre plates and Congo red agar, were compared with a PCR technique using 94 S. aureus strains obtained from cows with subclinical mastitis from two farms in the state of São Paulo. These strains were characterised by in vitro slime production on Congo red agar, biofilm formation on microtitre plates and the presence of the icaA and icaD genes. The results revealed that 85% of the isolates tested produced slime on the Congo red agar, 98.9% of the isolates produced biofilms in vitro by adhering to sterile 96-well "U" bottom polystyrene tissue culture plates, and 95.7% of the isolates carried the icaA and icaD genes. The results of the phenotypic tests for biofilm formation were compared with those of the molecular analysis, and the sensitivity and specificity of the Congo red agar test were 88.9% and 100%, respectively, while those of the microtitre plate test were 100% and 25%, respectively. When the phenotypic methods for the detection of biofilm producers, namely growth on microtitre plates and Congo red agar, were compared, the sensitivity and specificity were 86% and 100%, respectively. Therefore, growth on Congo red agar and the microtitre plate test are methods that could be used to determine whether an isolate has the potential for biofilm production. MenosAbstract: Biofilm formation is considered to be a selective advantage for Staphylococcus aureus mastitis isolates by facilitating bacterial persistence in the udder. It requires attachment to mammary epithelium, proliferation and accumulation of cells in multilayers. The objective of this study was to determine the sensitivity and specificity of three techniques for the detection of S. aureus biofilm-positive strains. Two phenotypic tests, including growth on microtitre plates and Congo red agar, were compared with a PCR technique using 94 S. aureus strains obtained from cows with subclinical mastitis from two farms in the state of São Paulo. These strains were characterised by in vitro slime production on Congo red agar, biofilm formation on microtitre plates and the presence of the icaA and icaD genes. The results revealed that 85% of the isolates tested produced slime on the Congo red agar, 98.9% of the isolates produced biofilms in vitro by adhering to sterile 96-well "U" bottom polystyrene tissue culture plates, and 95.7% of the isolates carried the icaA and icaD genes. The results of the phenotypic tests for biofilm formation were compared with those of the molecular analysis, and the sensitivity and specificity of the Congo red agar test were 88.9% and 100%, respectively, while those of the microtitre plate test were 100% and 25%, respectively. When the phenotypic methods for the detection of biofilm producers, namely growth on microtitre plates and Congo red agar, we... Mostrar Tudo |
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Palavras-Chave: |
Biofilms; Mastite bovina; Molecular analysis; Phenotypic; Phenotypic and molecular analysis. |
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Thesagro: |
Biofilme; Bovino; Doença animal; Estirpe; Mamite; Staphylococcus aureus. |
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Thesaurus Nal: |
Bovine mastitis; Mammary gland diseases. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/84964/1/PROCI-2013.00046.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/84668/1/API-Comparison-of-methods.pdf
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Marc: |
LEADER 02819naa a2200349 a 4500
001 1960826
005 2023-05-12
008 2013 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/S1517-83822013005000031$2DOI
100 1 $aMELO, P. de C.
245 $aComparison of methods for the detection of biofilm formation by Staphylococcus aureus isolated from bovine subclinical mastitis.$h[electronic resource]
260 $c2013
520 $aAbstract: Biofilm formation is considered to be a selective advantage for Staphylococcus aureus mastitis isolates by facilitating bacterial persistence in the udder. It requires attachment to mammary epithelium, proliferation and accumulation of cells in multilayers. The objective of this study was to determine the sensitivity and specificity of three techniques for the detection of S. aureus biofilm-positive strains. Two phenotypic tests, including growth on microtitre plates and Congo red agar, were compared with a PCR technique using 94 S. aureus strains obtained from cows with subclinical mastitis from two farms in the state of São Paulo. These strains were characterised by in vitro slime production on Congo red agar, biofilm formation on microtitre plates and the presence of the icaA and icaD genes. The results revealed that 85% of the isolates tested produced slime on the Congo red agar, 98.9% of the isolates produced biofilms in vitro by adhering to sterile 96-well "U" bottom polystyrene tissue culture plates, and 95.7% of the isolates carried the icaA and icaD genes. The results of the phenotypic tests for biofilm formation were compared with those of the molecular analysis, and the sensitivity and specificity of the Congo red agar test were 88.9% and 100%, respectively, while those of the microtitre plate test were 100% and 25%, respectively. When the phenotypic methods for the detection of biofilm producers, namely growth on microtitre plates and Congo red agar, were compared, the sensitivity and specificity were 86% and 100%, respectively. Therefore, growth on Congo red agar and the microtitre plate test are methods that could be used to determine whether an isolate has the potential for biofilm production.
650 $aBovine mastitis
650 $aMammary gland diseases
650 $aBiofilme
650 $aBovino
650 $aDoença animal
650 $aEstirpe
650 $aMamite
650 $aStaphylococcus aureus
653 $aBiofilms
653 $aMastite bovina
653 $aMolecular analysis
653 $aPhenotypic
653 $aPhenotypic and molecular analysis
700 1 $aFERREIRA, L. M.
700 1 $aNADER FILHO, A.
700 1 $aZAFALON, L. F.
700 1 $aVICENTE, H. I. G.
700 1 $aSOUZA, V. de
773 $tBrazilian Journal of Microbiology$gv. 44, n. 1, p. 119-124, 2013.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| 1. |  | MELO, P. de C.; FERREIRA, L. M.; NADER FILHO, A.; ZAFALON, L. F.; VICENTE, H. I. G.; SOUZA, V. de. Comparison of methods for the detection of biofilm formation by Staphylococcus aureus isolated from bovine subclinical mastitis. Brazilian Journal of Microbiology, v. 44, n. 1, p. 119-124, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Pecuária Sudeste. |
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