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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
25/06/2013 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ARAUJO, A. C. G. de; MORGANTE, C. V.; GUIMARAES, P. M.; SILVA JUNIOR, O. B. da; ROBERTS, P. A.; BERTIOLI, S. C. de M. L.; BERTIOLI, D.; BRASILEIRO, A. C. M. |
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Afiliação: |
ANA CLAUDIA GUERRA DE ARAUJO, Cenargen; CAROLINA VIANNA MORGANTE, CPATSA; PATRICIA MESSEMBERG GUIMARAES, Cenargen; ORZENIL BONFIM DA SILVA JUNIOR, CENARGEN; SORAYA CRISTINA DE M LEAL BERTIOLI, CENARGEN; UnB; ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN. |
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Título: |
A survey of differentially expressed genes in resistant wild arachis during nematode infection. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 4., 2013, Bento Gonçalves. Resumos... Bento Gonçalves: SBG, 2013. |
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Páginas: |
p. 58. |
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Idioma: |
Inglês |
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Conteúdo: |
The plant parasitic root-knot nematode evolved sophisticated strategies for exploiting plants and causes devastating yield penalties worldwide. The main control strategy of this parasite is the use of nematicides, which are contaminants for groundwater systems and toxic for human health. Until now, relatively little is known about crucial events related to the establishment and eproduction of these nematodes in peanut (Arachis hypogaea), and even less about the resistance mechanisms on this plant host. Wild relatives of crop species are an important source of genes for resistance to biotic stresses and, in particular, the wild peanut relative Arachis stenosperma shows high levels of resistance to Meloidogyne arenaria and other parasites. A genome-wide overview of gene expression during this plant nematode interaction at different time points of interaction [3, 6 and 9 days after inoculation (DAI)] was conducted using Illumina Hi-Seq 2000. For that, eight cDNA libraries were constructed using total RNA from inoculated and non inoculated roots of A. stenorpema plants, in two biological replicates. The total sequence data produced 38,241.633 reads with an average sequence size of 200 bp that were assembled into 44,133 contigs. Ten clusters were further identified by K-means clustering (MEV program) based on their expression patterns and in silico analysis showed a comparable number of genes that were significantly up- and down-regulated during the resistance response. Sequences were GO categorized and genes related to diverse classes of function and process in the cell were identified. Based on these analyses, defence-related genes, transcription factors, components of hormonal signaling pathways and genes involved on cell wall metabolism were further selected for qRT-PCR analysis to validate their expression profile. A number of genes showed comparable expression pattern on both qRT-PCR and in silico analysis. The transcriptome data obtained in this study is a helpful resource to identify genes regulated during early response of resistant Arachis species to M. arenaria which will help the understanding of the resistance mechanisms and the selection of candidate genes to be validated via plant transformation. A number of SSR and SNP markers will also be developed from these data providing new tools for peanut breeding program. MenosThe plant parasitic root-knot nematode evolved sophisticated strategies for exploiting plants and causes devastating yield penalties worldwide. The main control strategy of this parasite is the use of nematicides, which are contaminants for groundwater systems and toxic for human health. Until now, relatively little is known about crucial events related to the establishment and eproduction of these nematodes in peanut (Arachis hypogaea), and even less about the resistance mechanisms on this plant host. Wild relatives of crop species are an important source of genes for resistance to biotic stresses and, in particular, the wild peanut relative Arachis stenosperma shows high levels of resistance to Meloidogyne arenaria and other parasites. A genome-wide overview of gene expression during this plant nematode interaction at different time points of interaction [3, 6 and 9 days after inoculation (DAI)] was conducted using Illumina Hi-Seq 2000. For that, eight cDNA libraries were constructed using total RNA from inoculated and non inoculated roots of A. stenorpema plants, in two biological replicates. The total sequence data produced 38,241.633 reads with an average sequence size of 200 bp that were assembled into 44,133 contigs. Ten clusters were further identified by K-means clustering (MEV program) based on their expression patterns and in silico analysis showed a comparable number of genes that were significantly up- and down-regulated during the resistance response. Sequences... Mostrar Tudo |
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Palavras-Chave: |
Nematóide das galhas; Root-knot nematode. |
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Thesagro: |
Amendoim; Doença. |
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Thesaurus Nal: |
Arachis; Meloidogyne. |
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Categoria do assunto: |
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/84814/1/Carolina-2013-1.pdf
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Marc: |
LEADER 03257nam a2200277 a 4500
001 1960580
005 2024-02-05
008 2013 bl uuuu u00u1 u #d
100 1 $aARAUJO, A. C. G. de
245 $aA survey of differentially expressed genes in resistant wild arachis during nematode infection.$h[electronic resource]
260 $aIn: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 4., 2013, Bento Gonçalves. Resumos... Bento Gonçalves: SBG$c2013
300 $ap. 58.
520 $aThe plant parasitic root-knot nematode evolved sophisticated strategies for exploiting plants and causes devastating yield penalties worldwide. The main control strategy of this parasite is the use of nematicides, which are contaminants for groundwater systems and toxic for human health. Until now, relatively little is known about crucial events related to the establishment and eproduction of these nematodes in peanut (Arachis hypogaea), and even less about the resistance mechanisms on this plant host. Wild relatives of crop species are an important source of genes for resistance to biotic stresses and, in particular, the wild peanut relative Arachis stenosperma shows high levels of resistance to Meloidogyne arenaria and other parasites. A genome-wide overview of gene expression during this plant nematode interaction at different time points of interaction [3, 6 and 9 days after inoculation (DAI)] was conducted using Illumina Hi-Seq 2000. For that, eight cDNA libraries were constructed using total RNA from inoculated and non inoculated roots of A. stenorpema plants, in two biological replicates. The total sequence data produced 38,241.633 reads with an average sequence size of 200 bp that were assembled into 44,133 contigs. Ten clusters were further identified by K-means clustering (MEV program) based on their expression patterns and in silico analysis showed a comparable number of genes that were significantly up- and down-regulated during the resistance response. Sequences were GO categorized and genes related to diverse classes of function and process in the cell were identified. Based on these analyses, defence-related genes, transcription factors, components of hormonal signaling pathways and genes involved on cell wall metabolism were further selected for qRT-PCR analysis to validate their expression profile. A number of genes showed comparable expression pattern on both qRT-PCR and in silico analysis. The transcriptome data obtained in this study is a helpful resource to identify genes regulated during early response of resistant Arachis species to M. arenaria which will help the understanding of the resistance mechanisms and the selection of candidate genes to be validated via plant transformation. A number of SSR and SNP markers will also be developed from these data providing new tools for peanut breeding program.
650 $aArachis
650 $aMeloidogyne
650 $aAmendoim
650 $aDoença
653 $aNematóide das galhas
653 $aRoot-knot nematode
700 1 $aMORGANTE, C. V.
700 1 $aGUIMARAES, P. M.
700 1 $aSILVA JUNIOR, O. B. da
700 1 $aROBERTS, P. A.
700 1 $aBERTIOLI, S. C. de M. L.
700 1 $aBERTIOLI, D.
700 1 $aBRASILEIRO, A. C. M.
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Embrapa Semiárido (CPATSA) |
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| 1. |  | ARAUJO, A. C. G. de; MORGANTE, C. V.; GUIMARAES, P. M.; SILVA JUNIOR, O. B. da; ROBERTS, P. A.; BERTIOLI, S. C. de M. L.; BERTIOLI, D.; BRASILEIRO, A. C. M. A survey of differentially expressed genes in resistant wild arachis during nematode infection. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 4., 2013, Bento Gonçalves. Resumos... Bento Gonçalves: SBG, 2013. p. 58.| Tipo: Resumo em Anais de Congresso |
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