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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
05/03/2013 |
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Data da última atualização: |
03/03/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
GUIMARAES, P. M.; MORGANTE, C. V.; ROBERTS, P. A.; SILVA JUNIOR, O. B. da; ARRAIS, L; BERTIOLI, S. C. de M. L.; ARAUJO, A. C. G. de; MORETZSOHN, M. de C.; BERTIOLI, D.; BRASILEIRO, A. C. M. |
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Afiliação: |
PATRICIA MESSEMBERG GUIMARAES, CENARGEN; CAROLINA VIANNA MORGANTE, CPATSA; University of California Riverside; ORZENIL BONFIM DA SILVA JUNIOR, CENARGEN; ADAO LUIZ CLASEN LOPES, CPACT; SORAYA CRISTINA DE M LEAL BERTIOLI, CENARGEN; ANA CLAUDIA GUERRA DE ARAUJO, CENARGEN; MARCIO DE CARVALHO MORETZSOHN, CENARGEN; University of Brasilia; ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN. |
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Título: |
Gene expression revealed during the interaction of resistant Arachis stenosperma and Meloidogyne arenaria. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON LEGUME GENETICS AND GENOMICS, 6., 2012, Hyderabad, India. Programe & Abstract book...Hyderabad: ICRISAT, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
The plant-parasitic root-knot nematodes have evolved sophisticated strategies for exploiting plants with high impacts in agriculture worldwide. The wild
species Arachis stenosperma shows high levels of resistance to M. arenaria and other parasites. A genome-wide overview of differential gene expression
during this plant?nematode interaction at different time points (3, 6 and 9 DAI)and healthy roots was conducted using Illumina Hi-Seq 2000. Eight cDNA
libraries produced a total of 38´241´633 reads with an average sequence size of 200 bp and were assembled into 44,133 contigs. These unique sequences
were grouped into eight clusters corresponding to their expression profile estimated by RPKM values. We found that a comparable number of genes
were up- and down-regulated during the resistance response and genes belonging to the same family were often differentially regulated. Defencerelated
genes (calmodulin, patatin, DC1, RGAs and PR), transcription factors and components of hormonal signaling pathways (ARP, CKX) were further
characterized using qRT-PCR for validation of their expression profile. This study aimed to characterize genes regulated during early response of resistant
Arachis to M. arenaria, which in association with gene-expression analysis at the cellular level, in planta gene function validation and biochemical data, will
provide a set of candidate genes for plant transformation aiming nematode resistance in peanut. |
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Palavras-Chave: |
Arachis stenosperma. |
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Thesagro: |
Melhoramento genético vegetal; Meloidogyne Arenaria; Nematóide. |
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Categoria do assunto: |
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/77953/1/MORGANTE0001.pdf
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Marc: |
LEADER 02383nam a2200265 a 4500
001 1952198
005 2023-03-03
008 2012 bl uuuu u01u1 u #d
100 1 $aGUIMARAES, P. M.
245 $aGene expression revealed during the interaction of resistant Arachis stenosperma and Meloidogyne arenaria.$h[electronic resource]
260 $aIn: INTERNATIONAL CONFERENCE ON LEGUME GENETICS AND GENOMICS, 6., 2012, Hyderabad, India. Programe & Abstract book...Hyderabad: ICRISAT$c2012
520 $aThe plant-parasitic root-knot nematodes have evolved sophisticated strategies for exploiting plants with high impacts in agriculture worldwide. The wild species Arachis stenosperma shows high levels of resistance to M. arenaria and other parasites. A genome-wide overview of differential gene expression during this plant?nematode interaction at different time points (3, 6 and 9 DAI)and healthy roots was conducted using Illumina Hi-Seq 2000. Eight cDNA libraries produced a total of 38´241´633 reads with an average sequence size of 200 bp and were assembled into 44,133 contigs. These unique sequences were grouped into eight clusters corresponding to their expression profile estimated by RPKM values. We found that a comparable number of genes were up- and down-regulated during the resistance response and genes belonging to the same family were often differentially regulated. Defencerelated genes (calmodulin, patatin, DC1, RGAs and PR), transcription factors and components of hormonal signaling pathways (ARP, CKX) were further characterized using qRT-PCR for validation of their expression profile. This study aimed to characterize genes regulated during early response of resistant Arachis to M. arenaria, which in association with gene-expression analysis at the cellular level, in planta gene function validation and biochemical data, will provide a set of candidate genes for plant transformation aiming nematode resistance in peanut.
650 $aMelhoramento genético vegetal
650 $aMeloidogyne Arenaria
650 $aNematóide
653 $aArachis stenosperma
700 1 $aMORGANTE, C. V.
700 1 $aROBERTS, P. A.
700 1 $aSILVA JUNIOR, O. B. da
700 1 $aARRAIS, L
700 1 $aBERTIOLI, S. C. de M. L.
700 1 $aARAUJO, A. C. G. de
700 1 $aMORETZSOHN, M. de C.
700 1 $aBERTIOLI, D.
700 1 $aBRASILEIRO, A. C. M.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| Registros recuperados : 1 | |
| 1. |  | GUIMARAES, P. M.; MORGANTE, C. V.; ROBERTS, P. A.; SILVA JUNIOR, O. B. da; ARRAIS, L; BERTIOLI, S. C. de M. L.; ARAUJO, A. C. G. de; MORETZSOHN, M. de C.; BERTIOLI, D.; BRASILEIRO, A. C. M. Gene expression revealed during the interaction of resistant Arachis stenosperma and Meloidogyne arenaria. In: INTERNATIONAL CONFERENCE ON LEGUME GENETICS AND GENOMICS, 6., 2012, Hyderabad, India. Programe & Abstract book...Hyderabad: ICRISAT, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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