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Registro Completo |
Biblioteca(s): |
Embrapa Agrobiologia; Embrapa Semiárido. |
Data corrente: |
22/02/2013 |
Data da última atualização: |
09/01/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FERNANDES JUNIOR, P. I.; LIMA, A. A. de; PASSOS, S. R.; GAVA, C. A. T.; OLIVEIRA, P. J. de; RUMJANEK, N. G.; XAVIER, G. R. |
Afiliação: |
PAULO IVAN FERNANDES JUNIOR, CPATSA; ANDRÉA APARECIDA DE LIMA, UFRRJ; SAMUEL RIBEIRO PASSOS, UFRRJ; CARLOS ALBERTO TUAO GAVA, CPATSA; PAULO JANSEN DE OLIVEIRA, UFRRJ; NORMA GOUVEA RUMJANEK, CNPAB; GUSTAVO RIBEIRO XAVIER, CNPAB. |
Título: |
Phenotypic diversity and amylolytic activity of fast growing rhizobia from pigeonpea [Cajanus cajan (L.) Millsp.]. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Brazilian Journal of Microbiology, São Paulo, v. 43, n. 4, p. 1604-1612, 2012. |
ISSN: |
1517-8382 |
Idioma: |
Inglês |
Conteúdo: |
This study evaluated 26 pigeonpea rhizobial isolates according to their cultural characteristics, intrinsic antibiotic resistance, salt and temperature tolerance, carbon source utilization and amylolytic activity. The cultural characterization showed that the majority of them presented the ability to acidify the YMA. Among the 27 isolates evaluated, 25 were able to grow when incubated at 42° C and 11 showed tolerance to 3% (w/v) of NaCl in YMA medium. The patterns of carbon sources utilization was very diverse among the isolates. It was observed the capacity of three strains to metabolize all the carbon sources evaluated and a total of 42% of the bacterial isolates was able to grow in the culture medium supplemented with at least, six carbon sources. The carbon sources mannitol (control) and sucrose were metabilized by all isolates evaluated. The profile of intrinsic resistance to antibiotics showed that the isolates were mostly resistant to streptomycin and ampicillin, but susceptible to kanamycin and chloranphenicol. High amylolytic activity of, at least, four isolates was also demonstrated, especially for isolated 47.3b, which showed the highest enzymatic index. These results indicate the metabolic versatility of the pigeonpea rhizobia, and indicates the isolate 47.3b to further studies regarding the amylase production and characterization |
Palavras-Chave: |
Biological nitrogen fixation; Diversidade fenotípica; FBN; Fixação biológica de nitrogênio; Rizóbio; Seleção de estirpe; Seleção de estirpes; Strain selection. |
Thesagro: |
Feijão; Feijão de Corda; Melhoramento Vegetal. |
Thesaurus Nal: |
biotechnology; Molecular genetics; Nitrogen fixation; Pigeon peas. |
Categoria do assunto: |
-- F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/78769/1/Paulo-Ivan-2012.pdf
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Marc: |
LEADER 02518naa a2200385 a 4500 001 1950566 005 2023-01-09 008 2012 bl uuuu u00u1 u #d 022 $a1517-8382 100 1 $aFERNANDES JUNIOR, P. I. 245 $aPhenotypic diversity and amylolytic activity of fast growing rhizobia from pigeonpea [Cajanus cajan (L.) Millsp.]. 260 $c2012 520 $aThis study evaluated 26 pigeonpea rhizobial isolates according to their cultural characteristics, intrinsic antibiotic resistance, salt and temperature tolerance, carbon source utilization and amylolytic activity. The cultural characterization showed that the majority of them presented the ability to acidify the YMA. Among the 27 isolates evaluated, 25 were able to grow when incubated at 42° C and 11 showed tolerance to 3% (w/v) of NaCl in YMA medium. The patterns of carbon sources utilization was very diverse among the isolates. It was observed the capacity of three strains to metabolize all the carbon sources evaluated and a total of 42% of the bacterial isolates was able to grow in the culture medium supplemented with at least, six carbon sources. The carbon sources mannitol (control) and sucrose were metabilized by all isolates evaluated. The profile of intrinsic resistance to antibiotics showed that the isolates were mostly resistant to streptomycin and ampicillin, but susceptible to kanamycin and chloranphenicol. High amylolytic activity of, at least, four isolates was also demonstrated, especially for isolated 47.3b, which showed the highest enzymatic index. These results indicate the metabolic versatility of the pigeonpea rhizobia, and indicates the isolate 47.3b to further studies regarding the amylase production and characterization 650 $abiotechnology 650 $aMolecular genetics 650 $aNitrogen fixation 650 $aPigeon peas 650 $aFeijão 650 $aFeijão de Corda 650 $aMelhoramento Vegetal 653 $aBiological nitrogen fixation 653 $aDiversidade fenotípica 653 $aFBN 653 $aFixação biológica de nitrogênio 653 $aRizóbio 653 $aSeleção de estirpe 653 $aSeleção de estirpes 653 $aStrain selection 700 1 $aLIMA, A. A. de 700 1 $aPASSOS, S. R. 700 1 $aGAVA, C. A. T. 700 1 $aOLIVEIRA, P. J. de 700 1 $aRUMJANEK, N. G. 700 1 $aXAVIER, G. R. 773 $tBrazilian Journal of Microbiology, São Paulo$gv. 43, n. 4, p. 1604-1612, 2012.
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Embrapa Semiárido (CPATSA) |
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1. | | FERNANDES JUNIOR, P. I.; LIMA, A. A. de; PASSOS, S. R.; GAVA, C. A. T.; OLIVEIRA, P. J. de; RUMJANEK, N. G.; XAVIER, G. R. Phenotypic diversity and amylolytic activity of fast growing rhizobia from pigeonpea [Cajanus cajan (L.) Millsp.]. Brazilian Journal of Microbiology, São Paulo, v. 43, n. 4, p. 1604-1612, 2012.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Agrobiologia; Embrapa Semiárido. |
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